FastQCFastQC Report
Wed 22 Oct 2014
SV6_CGATGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV6_CGATGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24284623
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC414120.1705276627106791TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT54800.036.45974742
TCTCGTA54850.036.303341
CGATGTA55300.035.8870834
CACCGAT55400.035.6580831
TATCTCG56000.035.3182439
TCGTATG57600.034.6874743
CCGATGT57750.034.4406133
ACCGATG57500.034.43390332
CGTATGC59200.033.74920344
ATCTCGT60000.033.30120540
GTCACCG62650.031.78290629
CGTCTGA65250.031.30864116
ACACGTC66150.031.1547713
TCACCGA64000.031.11254930
ACGTCTG70200.029.45352615
GATCGGA72800.029.12191
ATCGGAA74100.028.7279832
CACGTCT80750.025.68900514
TCGGAAG83250.025.5967853
CGGAAGA84600.025.0287384
Produced by FastQC (version 0.11.2)