FastQCFastQC Report
Wed 22 Oct 2014
SV68_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV68_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31027558
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC758320.2444020892652912TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG100700.036.37450443
CTCGTAT101050.036.20386542
CGTATGC104700.034.9851244
TCTCGTA105050.034.8467541
AATCTCG107850.033.96291739
ATCTCGT112550.032.52466240
ACACGTC118900.032.22583813
CGTCTGA120700.031.52156416
ATCGGAA128400.030.6364782
ACGTCTG126450.029.99923315
GTATGCC123400.029.82934845
GATCGGA133300.029.565481
GTAATCT129700.028.1892937
CACGTCT137500.027.7520214
CACACGT144300.026.75607312
TCGGAAG150400.026.3940143
CGGAAGA152150.025.898164
TGTAATC145200.025.53649536
TAATCTC151900.024.3953238
TTGTAAT156550.023.6132235
Produced by FastQC (version 0.11.2)