FastQCFastQC Report
Wed 22 Oct 2014
SV67_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV67_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27959871
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1195670.42763788144802245TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT147500.038.10384442
TCGTATG149200.037.8054743
TCTCGTA150200.037.44884541
CGTATGC153100.036.82819444
AATCTCG154750.036.2750739
ATCTCGT158750.035.48860540
ACACGTC166750.035.1488413
ATCGGAA173800.034.5210152
CGTCTGA171350.034.0476816
GATCGGA178650.033.6028061
GTATGCC169300.033.41049245
ACGTCTG177700.032.90697515
GACCAAT171950.032.63342335
CACGTCT186100.031.47001314
ACCAATC178700.031.35040536
TCGGAAG195400.030.8543723
CACACGT192500.030.77437812
CGGAAGA203750.029.634094
CCAATCT194000.029.10987537
CAATCTC199100.028.31901638
Produced by FastQC (version 0.11.2)