FastQCFastQC Report
Wed 22 Oct 2014
SV66_TGACCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV66_TGACCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22813030
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC5607742.458130287822354TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA631650.042.9442632
GATCGGA632900.042.9011151
ACACGTC629500.042.266713
CTCGTAT618350.042.24216542
TCGTATG620550.042.20834443
TCTCGTA620650.042.0708541
CGTATGC622900.042.0484644
CGTCTGA633800.041.96219616
AATCTCG623100.041.91050739
TCGGAAG648650.041.7752153
ACGTCTG636650.041.7672815
ATCTCGT625350.041.7525240
GTATGCC628050.041.68933545
CACGTCT640550.041.53405414
CACACGT650550.041.05123512
CGGAAGA659450.041.005744
GACCAAT637150.040.95364435
ACCAATC640900.040.63345336
GCACACG665650.040.1706211
CCAATCT654500.039.82358637
Produced by FastQC (version 0.11.2)