FastQCFastQC Report
Wed 22 Oct 2014
SV25b_CAGATC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV25b_CAGATC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25193991
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC4150441.6473928247414233TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA451750.042.8282441
CTCGTAT452400.042.7869442
TCGTATG455550.042.60476343
CGTATGC455850.042.511844
CGTCTGA464450.042.3329216
ACACGTC465750.042.32586313
ATCGGAA472900.042.265722
GATCGGA473500.042.244061
ACGTCTG467350.042.11837415
CATCTCG463050.041.8089639
ATCTCGT463250.041.7666240
TCGGAAG480950.041.4958153
CACGTCT474700.041.48045714
CGGAAGA482100.041.3314864
GTATGCC470600.041.20326245
GCACACG482300.040.99941611
CACACGT486200.040.62889512
GATCATC488750.039.6329836
GAACTCC510250.038.34169821
AGTCACC507200.038.25691628
Produced by FastQC (version 0.11.2)