FastQCFastQC Report
Wed 22 Oct 2014
SV14_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV14_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16134327
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC302805518.767780025779818TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3213350.044.872161
ATCGGAA3217250.044.816142
TCGGAAG3218250.044.745443
CGGAAGA3215450.044.717924
ACACGTC3201750.044.53717813
GCACACG3211400.044.48938411
ACGTCTG3202250.044.45645515
CACACGT3211450.044.45112
CGTCTGA3202050.044.44025816
CACGTCT3208300.044.41750314
GAGCACA3246000.044.133699
GAAGAGC3255250.044.123566
AGAGCAC3251300.044.1072738
AGCACAC3251050.044.0035410
GTATGCC3194350.043.98414645
GTCACGC3208050.043.92357329
AGTCACG3207800.043.92068528
CGCCAAT3200850.043.91215533
ACGCCAA3208750.043.87766632
TCTGAAC3236900.043.8658718
Produced by FastQC (version 0.11.2)