FastQCFastQC Report
Wed 22 Oct 2014
SV13_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV13_ACAGTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28804005
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1804680.6265378720771643TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT204750.041.11996542
TCTCGTA205450.040.93605841
TCGTATG206550.040.8817343
GATCTCG206200.040.7763239
CGTATGC207650.040.57876244
ATCTCGT211050.039.9352240
CGTCTGA216100.039.6680516
GATCGGA222350.039.1943441
ACACGTC219000.039.15303413
ATCGGAA223500.039.0371972
ACGTCTG221750.038.71821615
CACGTCT230350.037.3117614
TCGGAAG234750.037.088873
CGGAAGA236500.036.7954034
GTATGCC232200.036.42411445
GCACACG243850.035.41213211
CACACGT244100.035.36670712
AGTGATC245850.034.1174936
GTGATCT256000.032.71216637
TGATCTC269350.031.19106538
Produced by FastQC (version 0.11.2)