FastQCFastQC Report
Wed 22 Oct 2014
SV11_CGATGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV11_CGATGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23479319
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC924890.3939168763795918TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT106650.039.72463242
TCTCGTA107150.039.51826541
CGTATGC107750.039.4864744
ACCGATG107250.039.43945732
CGATGTA106850.039.41864834
CACCGAT107350.039.40271831
TATCTCG108350.039.2259539
TCGTATG108550.039.112343
CCGATGT109450.038.5439233
CGTCTGA113150.038.3970716
GATCGGA116600.037.7573621
ACACGTC116550.037.54721513
ATCTCGT113050.037.5354440
GTCACCG113250.037.52875529
ATCGGAA118300.037.342232
TCACCGA113500.037.3073330
ACGTCTG120300.036.32068315
TCGGAAG124950.035.35433
CACGTCT125400.034.93323514
CGGAAGA128150.034.5241624
Produced by FastQC (version 0.11.2)