##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Complete_Undetermined_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99743465 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.136675660906707 33.0 27.0 33.0 15.0 33.0 2 29.39465672262338 33.0 27.0 33.0 15.0 33.0 3 30.057550848068093 33.0 27.0 33.0 27.0 33.0 4 35.122395617597604 37.0 33.0 37.0 33.0 37.0 5 35.8172892329337 37.0 37.0 37.0 33.0 37.0 6 36.283907201338955 37.0 37.0 37.0 33.0 37.0 7 21.474637451185398 37.0 2.0 37.0 2.0 37.0 8 27.14587478989225 37.0 15.0 37.0 15.0 37.0 9 34.0712610896363 37.0 33.0 37.0 27.0 37.0 10-11 36.35260637376093 37.0 37.0 37.0 35.0 37.0 12-13 36.55948000703604 37.0 37.0 37.0 37.0 37.0 14-15 38.730667547994244 40.0 37.0 40.0 37.0 40.0 16-17 38.72121049233652 40.0 37.0 40.0 37.0 40.0 18-19 38.62910010194653 40.0 37.0 40.0 37.0 40.0 20-21 38.627535718756114 40.0 37.0 40.0 37.0 40.0 22-23 38.41643896670323 40.0 37.0 40.0 37.0 40.0 24-25 38.53515161118575 40.0 37.0 40.0 37.0 40.0 26-27 38.523507850865215 40.0 37.0 40.0 37.0 40.0 28-29 38.50877792344591 40.0 37.0 40.0 37.0 40.0 30-31 38.41634430385991 40.0 37.0 40.0 37.0 40.0 32-33 38.36268606168835 40.0 37.0 40.0 37.0 40.0 34-35 38.334352405944585 40.0 37.0 40.0 37.0 40.0 36-37 38.3162404624704 40.0 37.0 40.0 37.0 40.0 38-39 38.245129262353174 40.0 37.0 40.0 37.0 40.0 40-41 38.092276651908975 40.0 37.0 40.0 35.0 40.0 42-43 38.012119956931514 40.0 37.0 40.0 33.0 40.0 44-45 37.91092022419714 40.0 37.0 40.0 33.0 40.0 46-47 37.77822623266597 40.0 37.0 40.0 33.0 40.0 48-49 37.63451124843116 40.0 37.0 40.0 33.0 40.0 50-51 37.46155635359169 40.0 37.0 40.0 33.0 40.0 52-53 37.307123469191694 40.0 37.0 40.0 33.0 40.0 54-55 37.116097039540385 38.5 37.0 40.0 33.0 40.0 56-57 36.8711293566952 37.0 37.0 40.0 33.0 40.0 58-59 36.64305884099775 37.0 37.0 40.0 33.0 40.0 60-61 36.36048467435937 37.0 37.0 40.0 33.0 40.0 62-63 36.114064685841825 37.0 37.0 40.0 33.0 40.0 64-65 35.80880520342862 37.0 35.0 38.5 33.0 40.0 66-67 35.4528040759362 37.0 33.0 37.0 33.0 40.0 68-69 35.134936088294104 37.0 33.0 37.0 33.0 40.0 70-71 34.85285375838908 37.0 33.0 37.0 27.0 40.0 72-73 34.589807858590035 37.0 33.0 37.0 27.0 37.0 74-75 34.498019389039676 37.0 33.0 37.0 30.0 37.0 76-77 34.47091035989175 37.0 33.0 37.0 33.0 37.0 78-79 34.261603705064786 37.0 33.0 37.0 27.0 37.0 80-81 34.12260316001655 37.0 33.0 37.0 27.0 37.0 82-83 33.95786375578591 37.0 33.0 37.0 27.0 37.0 84-85 33.51090326067978 37.0 33.0 37.0 27.0 37.0 86-87 33.70563619380979 37.0 33.0 37.0 27.0 37.0 88-89 33.6116036474169 37.0 33.0 37.0 27.0 37.0 90-91 33.47564437930846 37.0 33.0 37.0 27.0 37.0 92-93 33.39972783680615 37.0 33.0 37.0 27.0 37.0 94-95 33.219851922128434 33.0 33.0 37.0 27.0 37.0 96-97 33.12350633698158 33.0 33.0 37.0 27.0 37.0 98-99 33.071598991472776 33.0 33.0 37.0 27.0 37.0 100-101 32.567952913005385 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 -0.6328982103908594 2201 2 -0.18783624273440225 2201 3 0.8672145035390351 2201 4 0.8053710996182559 2201 5 0.632257561290352 2201 6 0.5152135541574125 2201 7 -17.828331682153937 2201 8 -11.119555454590724 2201 9 -2.6737245658909927 2201 10-11 -0.20036172348983428 2201 12-13 0.12041537165170979 2201 14-15 0.22599909601392199 2201 16-17 0.2710090090952164 2201 18-19 0.300229067102606 2201 20-21 0.2946868438823529 2201 22-23 0.278052645110634 2201 24-25 0.30123127243674475 2201 26-27 0.28623500003723734 2201 28-29 0.31747762660343426 2201 30-31 0.31736235403020174 2201 32-33 0.27214343438679833 2201 34-35 0.3419855644694536 2201 36-37 0.36553061647659746 2201 38-39 0.33653542562912264 2201 40-41 0.35030096007596256 2201 42-43 0.3398614695444735 2201 44-45 0.3507627718272346 2201 46-47 0.3572997609569981 2201 48-49 0.3433096331953891 2201 50-51 0.3296644184076172 2201 52-53 0.2937016172498872 2201 54-55 0.31869258205851025 2201 56-57 0.35218173215616844 2201 58-59 0.3282251353820058 2201 60-61 0.32481263163091256 2201 62-63 0.26946032081841054 2201 64-65 0.23120746286420513 2201 66-67 0.18311847972091044 2201 68-69 0.2463310189213317 2201 70-71 0.2960144960145996 2201 72-73 0.2327070482895479 2201 74-75 0.18005828902201415 2201 76-77 0.17105395245609856 2201 78-79 0.17862398391014267 2201 80-81 0.19815652989207422 2201 82-83 0.19609674496782503 2201 84-85 0.24166127654179093 2201 86-87 0.21928979219279654 2201 88-89 0.22179647553480208 2201 90-91 0.20578843650498868 2201 92-93 0.2253816441716623 2201 94-95 0.27401147448858154 2201 96-97 0.2804302020824565 2201 98-99 0.27370258148197024 2201 100-101 0.3203857863415607 1101 1 0.7583838203202227 1101 2 0.8285008942003209 1101 3 1.5779209141018669 1101 4 1.1996903059433563 1101 5 0.7727937769454911 1101 6 0.582415064451375 1101 7 -17.828331682153937 1101 8 -11.119555454590724 1101 9 -2.4066080522392177 1101 10-11 -0.08361617588036552 1101 12-13 0.13544531246217417 1101 14-15 0.23233662226596863 1101 16-17 0.2604636136145757 1101 18-19 0.26592161758398447 1101 20-21 0.2830914056824483 1101 22-23 0.31765249885893354 1101 24-25 0.2950187075010575 1101 26-27 0.348122535683153 1101 28-29 0.323892151253645 1101 30-31 0.3606773022064971 1101 32-33 0.32808151154128495 1101 34-35 0.34745861510850773 1101 36-37 0.3418000601442799 1101 38-39 0.33830693372421905 1101 40-41 0.3433793290552316 1101 42-43 0.3549193294897961 1101 44-45 0.3460260474862551 1101 46-47 0.36955036919122364 1101 48-49 0.407342671428772 1101 50-51 0.39209456473633253 1101 52-53 0.33312951987192463 1101 54-55 0.35016596662320865 1101 56-57 0.3863500495011465 1101 58-59 0.28121051152138676 1101 60-61 0.3280987407521181 1101 62-63 0.3499615451787008 1101 64-65 0.267016058762799 1101 66-67 0.25793784736917047 1101 68-69 0.29985170708296494 1101 70-71 0.3331957602601321 1101 72-73 0.27699520684767265 1101 74-75 0.30613750777727944 1101 76-77 0.22277596719759174 1101 78-79 0.21607553108107425 1101 80-81 0.23763465694275254 1101 82-83 0.2775645993781666 1101 84-85 0.27085930754085297 1101 86-87 0.2773182863404102 1101 88-89 0.2557030540055365 1101 90-91 0.31699297957946015 1101 92-93 0.3391838361006947 1101 94-95 0.33186522891603687 1101 96-97 0.38452675867365116 1101 98-99 0.3641288447255846 1101 100-101 0.39869256582146306 2202 1 -1.3621490601586927 2202 2 -0.9952260177241925 2202 3 0.6956150080554622 2202 4 0.6109350258310897 2202 5 0.450142313521539 2202 6 0.3541792874573346 2202 7 16.948196305181888 2202 8 10.568521173572229 2202 9 2.7032269407245337 2202 10-11 0.3237783115053219 2202 12-13 0.030491528317433847 2202 14-15 0.05580347569324573 2202 16-17 0.036742933217340124 2202 18-19 0.043364141670558354 2202 20-21 0.05256384560669147 2202 22-23 0.04433611952223515 2202 24-25 0.04045580987538955 2202 26-27 0.06650869619963373 2202 28-29 0.07513328516402851 2202 30-31 0.1106880775953698 2202 32-33 0.053390254235829104 2202 34-35 0.046531564008027715 2202 36-37 0.041986378449813344 2202 38-39 0.05498450940591937 2202 40-41 0.0928413596869504 2202 42-43 0.10475522340876608 2202 44-45 0.10360329151406233 2202 46-47 0.0920485651980485 2202 48-49 0.09769825637911111 2202 50-51 0.12151452222551029 2202 52-53 0.12933469734626613 2202 54-55 0.12346716552323045 2202 56-57 0.17076729495722276 2202 58-59 0.2011619126576889 2202 60-61 0.19257676358495956 2202 62-63 0.23863766086812888 2202 64-65 0.2564312595909044 2202 66-67 0.27406099773093473 2202 68-69 0.3166750015390889 2202 70-71 0.3657688745285981 2202 72-73 0.3791747640670309 2202 74-75 0.25344201191821014 2202 76-77 0.2824271870941857 2202 78-79 0.2847311881224215 2202 80-81 0.325388445816543 2202 82-83 0.35645684216601126 2202 84-85 0.3341940410543387 2202 86-87 0.2803548755386558 2202 88-89 0.24990879841154623 2202 90-91 0.2907631977894738 2202 92-93 0.29858047676275135 2202 94-95 0.34999067586606003 2202 96-97 0.35247016093472894 2202 98-99 0.2878587223862894 2202 100-101 0.34811523328850313 1102 1 -1.300080670196376 1102 2 -1.007076421917919 1102 3 0.7142793373120426 1102 4 0.7699464850781013 1102 5 0.4839304745058328 1102 6 0.37342439472084266 1102 7 16.72291460158241 1102 8 10.458179318648511 1102 9 2.5987554271643916 1102 10-11 0.23669642213464925 1102 12-13 0.026153083971387048 1102 14-15 0.06526372774099798 1102 16-17 -8.591588862287836E-4 1102 18-19 0.03135832253059334 1102 20-21 0.03689758093961615 1102 22-23 0.03469042819414625 1102 24-25 0.020464904960505237 1102 26-27 0.011672680392244672 1102 28-29 0.03756421935233334 1102 30-31 0.051231792261702935 1102 32-33 0.04662068119491636 1102 34-35 0.06143511832455317 1102 36-37 0.05709053811963827 1102 38-39 0.05380064003791318 1102 40-41 0.07084835541648005 1102 42-43 0.023256913832263137 1102 44-45 0.07143196263315588 1102 46-47 0.07782888968342405 1102 48-49 0.09834856227217159 1102 50-51 0.11096044765147184 1102 52-53 0.09194569179054923 1102 54-55 0.1153097716573015 1102 56-57 0.15627706382075957 1102 58-59 0.17747425573120523 1102 60-61 0.1991822455318939 1102 62-63 0.2249579330353697 1102 64-65 0.2203312533438151 1102 66-67 0.25997210357410694 1102 68-69 0.23536237886268196 1102 70-71 0.2667216565571593 1102 72-73 0.30609915134023424 1102 74-75 0.28093704169276634 1102 76-77 0.29376859603763705 1102 78-79 0.28348800690550036 1102 80-81 0.27486761389654646 1102 82-83 0.22972641525984017 1102 84-85 0.24932522593929463 1102 86-87 0.2294012762384412 1102 88-89 0.20396187834948165 1102 90-91 0.24879881117283276 1102 92-93 0.31380428009200756 1102 94-95 0.3173644540748839 1102 96-97 0.31310850927870604 1102 98-99 0.3155938481179774 1102 100-101 0.3114383014006705 2203 1 3.6055791746032355 2203 2 1.1549862045191297 2203 3 1.5564598847253812 2203 4 1.0613489766917397 2203 5 0.6317492740937283 2203 6 0.42592768836782824 2203 7 16.71219042399288 2203 8 10.463989690451719 2203 9 2.7599041902607553 2203 10-11 0.29799206728859673 2203 12-13 0.031455357964581765 2203 14-15 0.06079389394231782 2203 16-17 -0.012855406425870797 2203 18-19 0.01931131256964136 2203 20-21 0.019758955412711998 2203 22-23 0.02136256349434973 2203 24-25 0.01576253110427217 2203 26-27 0.05623508327381188 2203 28-29 0.029943693594823628 2203 30-31 0.05561121669337865 2203 32-33 0.03263828720553619 2203 34-35 0.06392821742853982 2203 36-37 0.04730867354015089 2203 38-39 0.03272197968228596 2203 40-41 0.06094378690301028 2203 42-43 -0.008673837789395122 2203 44-45 0.03920300622232986 2203 46-47 0.07382003233671952 2203 48-49 0.11815824243623751 2203 50-51 0.09049717049749972 2203 52-53 0.10893682862181464 2203 54-55 0.1381552861962021 2203 56-57 0.19715031591898935 2203 58-59 0.17159544687135053 2203 60-61 0.17761606192996027 2203 62-63 0.19587046034236266 2203 64-65 0.21450773247259747 2203 66-67 0.260349175795092 2203 68-69 0.2300692005611964 2203 70-71 0.25382079114363876 2203 72-73 0.3032084693688972 2203 74-75 0.291320598816867 2203 76-77 0.2947170081876891 2203 78-79 0.30107269208946263 2203 80-81 0.3421929257893126 2203 82-83 0.34766812462897434 2203 84-85 0.32922356857638846 2203 86-87 0.3288204901329266 2203 88-89 0.3487133165372853 2203 90-91 0.3640427489786191 2203 92-93 0.35686123179112883 2203 94-95 0.3443335122382294 2203 96-97 0.30317685729310995 2203 98-99 0.31171681077150026 2203 100-101 0.3724608179808513 1103 1 -0.915962894988585 1103 2 -0.3030598211114892 1103 3 0.7894269757362018 1103 4 0.693177464490887 1103 5 0.5326561799168132 1103 6 0.4751924192575956 1103 7 -17.828331682153937 1103 8 -11.119555454590724 1103 9 -2.497918027320253 1103 10-11 -0.1316248633844168 1103 12-13 0.10329972901664775 1103 14-15 0.1646005942217954 1103 16-17 0.18873597927347419 1103 18-19 0.22593566379681107 1103 20-21 0.2096069369773801 1103 22-23 0.223473953035203 1103 24-25 0.22660935623111556 1103 26-27 0.24746008783834128 1103 28-29 0.22458981316987092 1103 30-31 0.2767472724323099 1103 32-33 0.25955435107970004 1103 34-35 0.24857355525545444 1103 36-37 0.23177762308640837 1103 38-39 0.2560873006181126 1103 40-41 0.2048771996674148 1103 42-43 0.21100891156483925 1103 44-45 0.2713748658564441 1103 46-47 0.23598907296726424 1103 48-49 0.2817626521367629 1103 50-51 0.2641624901818531 1103 52-53 0.2586962746580781 1103 54-55 0.24198238360409619 1103 56-57 0.2498417966164581 1103 58-59 0.19479942474833223 1103 60-61 0.20087969871833877 1103 62-63 0.20927297456343297 1103 64-65 0.16673469059313106 1103 66-67 0.14568157218153743 1103 68-69 0.15486868524886432 1103 70-71 0.18085171145450119 1103 72-73 0.11083488653655849 1103 74-75 0.11267913526966566 1103 76-77 0.10042375273852855 1103 78-79 0.11526352983220534 1103 80-81 0.14766309226312302 1103 82-83 0.19477796834032546 1103 84-85 0.17512596653411805 1103 86-87 0.18315049783542747 1103 88-89 0.17783683108791593 1103 90-91 0.224794122017002 1103 92-93 0.23624799411289388 1103 94-95 0.26517137377344824 1103 96-97 0.26684194639483394 1103 98-99 0.2872083483633858 1103 100-101 0.3141946956541517 2204 1 -0.9629548218090349 2204 2 -0.6898326844051361 2204 3 0.786519830076017 2204 4 0.721488691624657 2204 5 0.5195413741172885 2204 6 0.34504831024604954 2204 7 16.905589279289657 2204 8 10.547279615276793 2204 9 2.7678940453923673 2204 10-11 0.29600154403247814 2204 12-13 6.816780334588657E-4 2204 14-15 0.021709216902522144 2204 16-17 -0.027985705585052756 2204 18-19 -0.04374350806176608 2204 20-21 -0.05483388618620211 2204 22-23 -0.1035820603834594 2204 24-25 -0.0443898761676067 2204 26-27 -0.03618462822139179 2204 28-29 -0.010834623416840827 2204 30-31 -0.007884739155407772 2204 32-33 -0.07301906641421141 2204 34-35 -0.030255189348984857 2204 36-37 -0.056336140284649616 2204 38-39 -0.07424265870356095 2204 40-41 -0.11607423543218687 2204 42-43 -0.08529003056534634 2204 44-45 -0.04101556508432225 2204 46-47 -0.011391109816820233 2204 48-49 0.018824102476962423 2204 50-51 0.01284445496118991 2204 52-53 0.01450643459862988 2204 54-55 0.04357140201590681 2204 56-57 0.0813067235485363 2204 58-59 0.029710230300125318 2204 60-61 0.053522974419976777 2204 62-63 0.06694880813745385 2204 64-65 0.09893564975821079 2204 66-67 0.12095599248253563 2204 68-69 0.1282076516248054 2204 70-71 0.1704473946394387 2204 72-73 0.2236573500168788 2204 74-75 0.16266423438988653 2204 76-77 0.19028829144170345 2204 78-79 0.17738494618772194 2204 80-81 0.15533803856599349 2204 82-83 0.2151407993810679 2204 84-85 0.22394544188711762 2204 86-87 0.20306379317655399 2204 88-89 0.1982733124989693 2204 90-91 0.22979257466984393 2204 92-93 0.19857040588415487 2204 94-95 0.22903723830344802 2204 96-97 0.26818905154571837 2204 98-99 0.22634431975608038 2204 100-101 0.2148116319104787 2205 1 -0.5609563903535317 2205 2 -0.12690606332930088 2205 3 1.0097996727797778 2205 4 0.8216825836680641 2205 5 0.5648010860563986 2205 6 0.46117650575207847 2205 7 -17.828331682153937 2205 8 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-1.8548245527672123 1215 90-91 -1.9024440062483308 1215 92-93 -1.8681706440202213 1215 94-95 -1.92413051542216 1215 96-97 -2.0445193442272895 1215 98-99 -1.9589947713705982 1215 100-101 -1.9646898812736424 1212 1 -0.0235791512537169 1212 2 -0.09074385551523889 1212 3 1.263816661649308 1212 4 0.7832756793845803 1212 5 0.5869219837928412 1212 6 0.34680846643899343 1212 7 -17.828331682153937 1212 8 -11.119555454590724 1212 9 -2.8243934255831 1212 10-11 -0.26520831240102183 1212 12-13 -0.028005359139463337 1212 14-15 -0.10690615801826908 1212 16-17 -0.05027119360401855 1212 18-19 -0.06794333316304346 1212 20-21 -0.052554900396017956 1212 22-23 -0.06387734246092691 1212 24-25 -0.047854868284566976 1212 26-27 -0.02466350494535874 1212 28-29 -0.05175666549025948 1212 30-31 -0.025231401163864575 1212 32-33 -0.05256095577426123 1212 34-35 -0.0418174609744284 1212 36-37 -0.06467354714331464 1212 38-39 -0.08717268479051654 1212 40-41 -0.07510299993342784 1212 42-43 -0.056585779011165016 1212 44-45 -0.07559321282227671 1212 46-47 -0.11297890819420076 1212 48-49 -0.047268815907848705 1212 50-51 -0.10509917401328295 1212 52-53 -0.1236708265648403 1212 54-55 -0.11592916350007698 1212 56-57 -0.11732210557980238 1212 58-59 -0.16601217666634938 1212 60-61 -0.20363360700941513 1212 62-63 -0.226684800097658 1212 64-65 -0.32838661787170054 1212 66-67 -0.33107715151624717 1212 68-69 -0.3531704197286345 1212 70-71 -0.31688704677019786 1212 72-73 -0.38002288570402243 1212 74-75 -0.3407982894976911 1212 76-77 -0.3529558552119738 1212 78-79 -0.39977842273947317 1212 80-81 -0.397406010263083 1212 82-83 -0.3745980640060722 1212 84-85 -0.3719209241919046 1212 86-87 -0.395862224991383 1212 88-89 -0.3979676398265042 1212 90-91 -0.40680893891492076 1212 92-93 -0.4187224349498422 1212 94-95 -0.42494998955627494 1212 96-97 -0.47439332355919817 1212 98-99 -0.4606900523284523 1212 100-101 -0.5040728920002806 1213 1 -4.584186697876731 1213 2 -2.4842159006179223 1213 3 -4.2347131153338715 1213 4 -3.390904225480849 1213 5 -1.812582559683662 1213 6 -0.6474585066278635 1213 7 16.455225998057408 1213 8 10.228229382076364 1213 9 2.5828175191685077 1213 10-11 0.08290007516693265 1213 12-13 -0.2466620616056474 1213 14-15 -0.4474852858263745 1213 16-17 -0.5069008802165911 1213 18-19 -0.5394333173922234 1213 20-21 -0.5411070732951515 1213 22-23 -0.5796814596249789 1213 24-25 -0.5807768128855528 1213 26-27 -0.5996723224873293 1213 28-29 -0.6095307415984337 1213 30-31 -0.6276737321044124 1213 32-33 -0.6290151846267733 1213 34-35 -0.7400547280454433 1213 36-37 -0.7281041482609751 1213 38-39 -0.7272033624430421 1213 40-41 -0.7479655363488646 1213 42-43 -0.7459163417717818 1213 44-45 -0.7062746118261387 1213 46-47 -0.6810191037546005 1213 48-49 -0.6452871734305461 1213 50-51 -0.717345951157867 1213 52-53 -0.8233925761598684 1213 54-55 -0.7274804931137453 1213 56-57 -0.6714611394497183 1213 58-59 -0.7091333956451038 1213 60-61 -0.7507471492189879 1213 62-63 -0.7484776532450326 1213 64-65 -0.7468442647901128 1213 66-67 -0.8210417957549652 1213 68-69 -0.823663931253293 1213 70-71 -0.807430775670575 1213 72-73 -0.8558878260261906 1213 74-75 -0.890899953123494 1213 76-77 -0.8858909698334543 1213 78-79 -0.8664348727411948 1213 80-81 -0.9430917693344014 1213 82-83 -0.9993870866425851 1213 84-85 -0.9992029197197425 1213 86-87 -1.0615196813967955 1213 88-89 -1.096558707222968 1213 90-91 -1.1565765980358478 1213 92-93 -1.2621737350322917 1213 94-95 -1.374583633125674 1213 96-97 -1.4784825707184588 1213 98-99 -1.5085414460013844 1213 100-101 -1.5917478771368536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 41.0 9 233.0 10 305.0 11 233.0 12 234.0 13 580.0 14 2186.0 15 6176.0 16 14662.0 17 29582.0 18 53317.0 19 88253.0 20 136213.0 21 193349.0 22 260280.0 23 349399.0 24 436582.0 25 514750.0 26 622092.0 27 775940.0 28 995063.0 29 1289957.0 30 1665638.0 31 2212290.0 32 3040495.0 33 4289755.0 34 6467771.0 35 1.0862554E7 36 2.0772909E7 37 3.3933109E7 38 1.0720402E7 39 9112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.38905479186833 20.73520482484334 28.243363133692366 27.63237724959596 2 22.630581632189305 5.469947936276529 25.24482957866536 46.65464085286881 3 14.240357544736678 33.73394651897687 24.831186282263953 27.194509654022497 4 20.554825321137578 27.11007683561023 22.228769574026728 30.106328269225457 5 38.222717926433766 24.425022934975658 17.371903695570747 19.980355443019832 6 25.180574988045585 37.608380659324396 28.143081855036815 9.0679624975932 7 0.4777397459612363 0.4627610747013006 98.46724266033966 0.5922565189978025 8 41.266151120777685 12.95564777100936 9.610101273301463 36.16809983491149 9 25.883715790302652 10.65561137263479 27.9115789691084 35.549093867954156 10-11 27.684976153575576 13.8792290803212 17.285731952464253 41.15006281363897 12-13 27.11605116184805 23.595565884942935 24.20438922991095 25.083993723298065 14-15 21.11590719251632 23.81857999418809 34.54867143426389 20.5168413790317 16-17 20.100488189386407 27.64390985702993 31.60457141938214 20.651030534201524 18-19 23.39154732834534 28.033935852729243 26.708159513722983 21.866357305202435 20-21 21.427063592804295 25.752161105049296 29.067288443124355 23.753486859022054 22-23 20.21166691350013 30.062151803903465 28.09897007283612 21.62721120976028 24-25 21.135300970389046 28.86746939272603 29.46782434910315 20.529405287781778 26-27 20.847880114985397 28.52250864324991 30.051812227356407 20.577799014408285 28-29 21.68733528221246 30.35683616078419 27.812145928632585 20.14368262837076 30-31 21.445938189399385 28.27213005759531 29.028842852481468 21.25308890052384 32-33 21.64244398594379 28.19530764235766 29.31463125914677 20.847617112551784 34-35 21.585772333783638 28.265916743921387 29.384115578640834 20.764195343654134 36-37 22.78102664028489 25.78888516860812 30.172511009852098 21.257577181254884 38-39 20.926009933361254 28.98644610497486 26.903928454725467 23.18361550693842 40-41 20.385209300232162 30.01474647533131 27.38493706848545 22.21510715595108 42-43 21.67871289145113 27.32311124340075 26.891575204048063 24.106600661100057 44-45 21.768037534626693 28.430454025666396 27.073926381187725 22.727582058519186 46-47 20.189814654789622 29.79638999934303 28.167429784216147 21.846365561651197 48-49 20.6420447656693 27.636351796295855 27.177206044744718 24.54439739329013 50-51 20.573225720364675 28.796431187401645 26.677289044613005 23.953054047620675 52-53 19.904906738501452 28.39781850768368 28.70759298327511 22.989681770539757 54-55 20.960050237015963 25.900724284602113 29.34541911717813 23.793806361203796 56-57 21.42939278569331 25.807454831737157 30.533933711552624 22.229218671016906 58-59 22.20197883182097 26.972840697328195 26.710035821620785 24.11514464923005 60-61 24.04414082309246 25.50619069311157 28.896827466065407 21.552841017730568 62-63 25.68607026034237 24.822959579356905 27.66548715747944 21.82548300282129 64-65 21.804334051632708 26.28363982665349 28.24988115804869 23.66214496366511 66-67 21.845481136345203 29.481854909637704 26.948034022169125 21.724629931847968 68-69 22.972883362950896 29.08143960300255 25.83663922914011 22.10903780490644 70-71 21.621613655125103 28.357588444090087 25.902530314763222 24.11826758602159 72-73 24.11105501118666 24.624642361310624 27.053631789565657 24.210670837937062 74-75 20.879783191045583 26.679959532454934 25.047294535783532 27.392962740715955 76-77 23.443621441956285 27.289568763430278 24.897422723895446 24.36938707071799 78-79 23.33764368597589 28.919560467854193 25.738881662634917 22.003914183535 80-81 25.695352430836397 26.377411363557556 25.585215577750485 22.34202062785556 82-83 25.976716844764137 27.218553133786465 26.571756601031804 20.232973420417597 84-85 23.726693665360244 24.03138718688992 29.688455226699727 22.55346392105011 86-87 22.00624529721256 27.550626258908846 26.581367364346615 23.861761079531988 88-89 22.003308530442037 28.31787023267196 26.236479874480032 23.44234136240597 90-91 22.17072376513258 26.38854324937058 28.24963624530309 23.191096740193753 92-93 23.25704003461456 27.278521800389605 26.288859477081555 23.17557868791428 94-95 21.87561535385021 29.78955870206223 25.754397471100926 22.580428472986636 96-97 23.30400049743644 29.600772265664588 25.78158106828446 21.31364616861451 98-99 23.055172045362553 28.19116942429986 25.86193445569695 22.891724074640635 100-101 22.584428051389562 28.272230401549724 28.24360403636712 20.89973751069359 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6.0 1 40.0 2 1212.5 3 42205.0 4 59797.5 5 27769.0 6 14274.5 7 9352.5 8 7216.0 9 5739.5 10 5063.0 11 4746.0 12 4718.0 13 5003.5 14 5273.0 15 5928.0 16 7517.5 17 12306.5 18 36215.5 19 89025.0 20 87175.0 21 60629.5 22 69909.5 23 82145.5 24 108572.5 25 131464.5 26 156160.0 27 208691.5 28 267962.0 29 345523.5 30 500735.5 31 613964.0 32 689460.0 33 810748.0 34 979731.5 35 1248322.5 36 1500578.5 37 1755205.0 38 2165851.0 39 2712534.0 40 3281009.0 41 3562965.5 42 3979341.5 43 4944789.0 44 5789358.5 45 6879491.0 46 7609059.5 47 8135666.0 48 7366367.5 49 5259182.0 50 4239756.5 51 3787881.0 52 3367078.5 53 3026322.5 54 2613541.5 55 2056226.0 56 1702438.0 57 1706726.5 58 1491497.0 59 1014074.5 60 715745.5 61 530203.0 62 425530.0 63 304166.5 64 231982.0 65 183333.5 66 151006.0 67 124954.5 68 101291.0 69 80916.0 70 63030.0 71 47728.0 72 36088.5 73 27228.5 74 19995.5 75 14498.0 76 10260.0 77 6741.5 78 4480.5 79 3044.0 80 2058.5 81 1430.5 82 937.0 83 612.0 84 413.5 85 281.0 86 192.5 87 148.5 88 115.0 89 92.0 90 98.0 91 113.5 92 108.0 93 98.5 94 89.5 95 78.5 96 106.0 97 145.0 98 197.0 99 744.5 100 1135.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.028632452261408804 2 0.003585197285857274 3 5.353734202035191E-4 4 0.0 5 0.0038879740141371665 6 0.0 7 43.76263848463656 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.00514389589333E-6 18-19 8.135871357587187E-4 20-21 2.2307225841813297E-4 22-23 1.0125976674261317E-4 24-25 2.2307225841813297E-4 26-27 0.0017625214844902372 28-29 0.00546501968825727 30-31 0.004711586869375352 32-33 0.005343708482555724 34-35 0.00199612074836181 36-37 0.0018938584096712503 38-39 0.004730635736386339 40-41 0.006767861934613963 42-43 0.004413823000835193 44-45 0.004853952085983779 46-47 0.0051306619436170585 48-49 0.005105597644918391 50-51 0.0018357092366903436 52-53 0.003493963238594127 54-55 0.0018768146865561568 56-57 6.516717661653323E-6 58-59 7.218518025215988E-5 60-61 1.5088707816597306E-4 62-63 0.0 64-65 7.002965056407455E-4 66-67 1.4838064829610643E-4 68-69 0.00197205902161109 70-71 0.0014311714556938643 72-73 0.002795170590875302 74-75 0.006272090106354335 76-77 0.006846062546553802 78-79 0.004925134694287992 80-81 0.003926573034133113 82-83 0.002909965078915195 84-85 0.0011108497183249049 86-87 9.374047713301317E-5 88-89 0.0022091672873004765 90-91 0.0028357747547671417 92-93 4.551676643677859E-4 94-95 0.0015685238125625574 96-97 6.917746440831988E-5 98-99 7.173402287558388E-4 100-101 0.0015008502060761574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9.9743465E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.33380078596672 #Duplication Level Percentage of deduplicated Percentage of total 1 53.40937424374882 9.257874532442838 2 17.314454546654794 6.0025061165877975 3 7.991656832151313 4.155773624349622 4 3.893551596840196 2.6996019091804224 5 2.664132367725244 2.3089769864797605 6 2.038041793164998 2.1196206261717876 7 1.8348105432828326 2.226296830607919 8 1.6653140823921109 2.309297804019985 9 1.0931798676833537 1.7054065844687438 >10 6.992006704427449 22.493115114114467 >50 0.6017200443198039 7.1620188388781205 >100 0.4199040857906312 14.499601921846923 >500 0.04744481067115639 5.6523695780259855 >1k 0.03128277013549809 10.231965860233073 >5k 0.0021842241864068117 2.6006114027850646 >10k+ 9.414868255293568E-4 4.574962269807755 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCAGANCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 334605 0.3354655866426938 No Hit ACTTGANCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 224714 0.22529195271088687 No Hit ACAGTGNCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 207117 0.20764969414286943 No Hit CGATGTNCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 170496 0.17093450683711459 No Hit CTCAGANGCCCACAGTAACGGACAGCTTTACCTGTTTCTATGGAGACAAC 139705 0.14006431398788882 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.203086337535998E-5 0.0 0.0 2 1.203086337535998E-5 0.0 0.0 3 1.203086337535998E-5 0.0 0.0 4 2.716969978935462E-4 0.0 0.0 5 2.7670985763327954E-4 0.0 0.0 6 2.7670985763327954E-4 2.00514389589333E-6 0.0 7 3.458873220415994E-4 2.00514389589333E-6 0.0 8 0.0017675343442299703 2.00514389589333E-6 0.0 9 0.0019379715753809034 2.00514389589333E-6 0.0 10-11 0.002174578555096316 2.00514389589333E-6 0.0 12-13 0.014490673649647123 2.00514389589333E-6 0.0 14-15 0.1703986321309371 2.00514389589333E-6 0.0 16-17 0.17049788675378383 2.00514389589333E-6 0.0 18-19 0.17058861951507298 2.00514389589333E-6 2.5064298698666626E-6 20-21 0.17066230855324707 2.00514389589333E-6 5.012859739733325E-6 22-23 0.17071895386830604 2.506429869866662E-6 7.0180036356266554E-6 24-25 0.17078512361687054 1.1028291427413314E-5 1.553986519317331E-5 26-27 0.17088588209763916 1.1028291427413314E-5 1.804629506303997E-5 28-29 0.17095305441815162 1.1028291427413314E-5 1.804629506303997E-5 30-31 0.17101220616308047 1.1028291427413314E-5 1.804629506303997E-5 32-33 0.17109291320489017 1.1028291427413314E-5 1.804629506303997E-5 34-35 0.17153905772172645 1.1028291427413314E-5 1.85475810370133E-5 36-37 0.17246743934552505 1.1028291427413314E-5 1.9048867010986634E-5 38-39 0.17457384300816098 1.1028291427413314E-5 1.9048867010986634E-5 40-41 0.17815202229038263 1.203086337535998E-5 1.9048867010986634E-5 42-43 0.18524973039587106 1.203086337535998E-5 1.9048867010986634E-5 44-45 0.1971251951192993 1.203086337535998E-5 2.00514389589333E-5 46-47 0.21177728285256583 1.203086337535998E-5 2.00514389589333E-5 48-49 0.2356435080734362 1.203086337535998E-5 2.00514389589333E-5 50-51 0.27234315551399785 1.203086337535998E-5 2.00514389589333E-5 52-53 0.3200039220614604 1.203086337535998E-5 2.00514389589333E-5 54-55 0.381948832437293 1.203086337535998E-5 2.00514389589333E-5 56-57 0.4496028887707079 1.203086337535998E-5 2.5064298698666623E-5 58-59 0.5302202003910732 1.203086337535998E-5 2.5064298698666623E-5 60-61 0.6249792906232002 1.203086337535998E-5 2.5064298698666623E-5 62-63 0.7445675764322004 1.203086337535998E-5 2.7069442594559955E-5 64-65 0.8937212077001736 1.3033435323306645E-5 2.7069442594559955E-5 66-67 1.0695803479456023 1.3033435323306645E-5 2.7069442594559955E-5 68-69 1.261269096677161 1.3033435323306645E-5 2.7069442594559955E-5 70-71 1.4760024629182473 1.3033435323306645E-5 3.057844441237328E-5 72-73 1.7368365937557915 1.3033435323306645E-5 3.408744623018661E-5 74-75 2.0366617502209294 1.3033435323306645E-5 3.408744623018661E-5 76-77 2.3799188247570906 1.3033435323306645E-5 3.408744623018661E-5 78-79 2.7552125846039135 1.3033435323306645E-5 3.408744623018661E-5 80-81 3.1383103644935533 1.3033435323306645E-5 3.408744623018661E-5 82-83 3.525722712761182 1.3033435323306645E-5 3.408744623018661E-5 84-85 3.960906611776521 1.3033435323306645E-5 3.408744623018661E-5 86-87 4.418545114710021 1.3033435323306645E-5 3.408744623018661E-5 88-89 4.915396211671611 1.3033435323306645E-5 3.408744623018661E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCAGAT 885495 0.0 158.47581 1 ACAGTGT 632340 0.0 150.77698 1 CGATGTT 654755 0.0 150.37587 1 GACCATG 306900 0.0 148.02547 2 GATCAGT 192825 0.0 146.92802 1 CTCAGTC 202080 0.0 146.20369 2 CTCAGTG 260815 0.0 146.18567 2 TCAGATG 325410 0.0 145.86063 2 GCTCAGT 313945 0.0 142.93173 1 GTGGCCT 329460 0.0 142.60825 1 TCTCAGT 273840 0.0 142.08195 1 CAGTGTG 294970 0.0 140.49644 2 ACTTGAT 587050 0.0 139.95427 1 GCCAATT 425765 0.0 138.92265 1 TCAGTCG 71150 0.0 137.77019 3 GATCTCC 114195 0.0 137.74712 5 TGGCCTC 191260 0.0 137.13043 2 AGTGTGC 135375 0.0 136.35834 3 TGGCCTG 232085 0.0 136.1709 2 TGACCAT 679290 0.0 134.01047 1 >>END_MODULE