FastQCFastQC Report
Wed 1 Oct 2014
Complete_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99743465
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGANCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA3346050.3354655866426938No Hit
ACTTGANCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA2247140.22529195271088687No Hit
ACAGTGNCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA2071170.20764969414286943No Hit
CGATGTNCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1704960.17093450683711459No Hit
CTCAGANGCCCACAGTAACGGACAGCTTTACCTGTTTCTATGGAGACAAC1397050.14006431398788882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT8854950.0158.475811
ACAGTGT6323400.0150.776981
CGATGTT6547550.0150.375871
GACCATG3069000.0148.025472
GATCAGT1928250.0146.928021
CTCAGTC2020800.0146.203692
CTCAGTG2608150.0146.185672
TCAGATG3254100.0145.860632
GCTCAGT3139450.0142.931731
GTGGCCT3294600.0142.608251
TCTCAGT2738400.0142.081951
CAGTGTG2949700.0140.496442
ACTTGAT5870500.0139.954271
GCCAATT4257650.0138.922651
TCAGTCG711500.0137.770193
GATCTCC1141950.0137.747125
TGGCCTC1912600.0137.130432
AGTGTGC1353750.0136.358343
TGGCCTG2320850.0136.17092
TGACCAT6792900.0134.010471
Produced by FastQC (version 0.11.2)