##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Complete_Undetermined_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95190794 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.56193835298821 33.0 27.0 33.0 15.0 33.0 2 29.547388185458356 33.0 27.0 33.0 15.0 33.0 3 30.0497998892624 33.0 27.0 33.0 22.0 33.0 4 35.12130782310735 37.0 33.0 37.0 33.0 37.0 5 35.72437562607157 37.0 37.0 37.0 33.0 37.0 6 36.227654588110695 37.0 37.0 37.0 33.0 37.0 7 20.431331773532637 37.0 2.0 37.0 2.0 37.0 8 26.517206453808967 37.0 15.0 37.0 15.0 37.0 9 33.93436987194371 33.0 33.0 37.0 27.0 37.0 10-11 36.371879364720925 37.0 37.0 37.0 35.0 37.0 12-13 36.60426838649964 37.0 37.0 37.0 37.0 37.0 14-15 38.83189215755465 40.0 37.0 40.0 37.0 40.0 16-17 38.82014097917914 40.0 37.0 40.0 37.0 40.0 18-19 38.73393429200728 40.0 37.0 40.0 37.0 40.0 20-21 38.73276750900933 40.0 37.0 40.0 37.0 40.0 22-23 38.54284724213982 40.0 37.0 40.0 37.0 40.0 24-25 38.65954177774796 40.0 37.0 40.0 37.0 40.0 26-27 38.66856214583103 40.0 37.0 40.0 37.0 40.0 28-29 38.63470561554513 40.0 37.0 40.0 37.0 40.0 30-31 38.582366221254546 40.0 37.0 40.0 37.0 40.0 32-33 38.51164085783337 40.0 37.0 40.0 37.0 40.0 34-35 38.479657927845416 40.0 37.0 40.0 37.0 40.0 36-37 38.450662187984264 40.0 37.0 40.0 37.0 40.0 38-39 38.379161329403345 40.0 37.0 40.0 37.0 40.0 40-41 38.23308278634592 40.0 37.0 40.0 37.0 40.0 42-43 38.14878282767554 40.0 37.0 40.0 37.0 40.0 44-45 38.0409598274808 40.0 37.0 40.0 35.0 40.0 46-47 37.91390731544901 40.0 37.0 40.0 33.0 40.0 48-49 37.811724976261885 40.0 37.0 40.0 33.0 40.0 50-51 37.5867171567032 40.0 37.0 40.0 33.0 40.0 52-53 37.43154009199671 40.0 37.0 40.0 33.0 40.0 54-55 37.24425679756385 38.5 37.0 40.0 33.0 40.0 56-57 37.026890615073555 37.0 37.0 40.0 33.0 40.0 58-59 36.765824292840755 37.0 37.0 40.0 33.0 40.0 60-61 36.47646253481193 37.0 37.0 40.0 33.0 40.0 62-63 36.21893220052351 37.0 37.0 40.0 33.0 40.0 64-65 35.91090003409363 37.0 35.0 38.5 33.0 40.0 66-67 35.557599493287135 37.0 33.0 37.0 33.0 40.0 68-69 35.22535701824275 37.0 33.0 37.0 33.0 40.0 70-71 34.989770801785724 37.0 33.0 37.0 33.0 40.0 72-73 34.70639067261063 37.0 33.0 37.0 33.0 37.0 74-75 34.592646422300035 37.0 33.0 37.0 30.0 37.0 76-77 34.551508499866074 37.0 33.0 37.0 33.0 37.0 78-79 34.34999769515527 37.0 33.0 37.0 30.0 37.0 80-81 34.20328025102931 37.0 33.0 37.0 27.0 37.0 82-83 34.03603486068201 37.0 33.0 37.0 27.0 37.0 84-85 33.638232117278065 37.0 33.0 37.0 27.0 37.0 86-87 33.81655863696231 37.0 33.0 37.0 27.0 37.0 88-89 33.69453527722439 37.0 33.0 37.0 27.0 37.0 90-91 33.57304224187898 37.0 33.0 37.0 27.0 37.0 92-93 33.50241206623405 37.0 33.0 37.0 27.0 37.0 94-95 33.302349211416384 35.0 33.0 37.0 27.0 37.0 96-97 33.170897051242164 33.0 33.0 37.0 27.0 37.0 98-99 33.15818411494708 33.0 33.0 37.0 27.0 37.0 100-101 32.68521314676711 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 2.8233965404306467 2201 2 0.8839761036287044 2201 3 1.3388139406159212 2201 4 0.875995152385066 2201 5 0.6911188856573887 2201 6 0.48222178253523396 2201 7 17.88921385380125 2201 8 11.223703517550089 2201 9 2.81369940764786 2201 10-11 0.35353778994562646 2201 12-13 0.06959233446902857 2201 14-15 0.20137550039269314 2201 16-17 0.1588487560364058 2201 18-19 0.10895478217388188 2201 20-21 0.12741510514536714 2201 22-23 0.11161907842502217 2201 24-25 0.10209156763286842 2201 26-27 0.12045907856001037 2201 28-29 0.11987877964326543 2201 30-31 0.12187294351400624 2201 32-33 0.15224167290029555 2201 34-35 0.15602003549836496 2201 36-37 0.153027569013247 2201 38-39 0.13991795536940543 2201 40-41 0.15112024007197533 2201 42-43 0.18413797760596395 2201 44-45 0.1717363078244034 2201 46-47 0.19296300443800618 2201 48-49 0.2181122150734751 2201 50-51 0.24830637728824456 2201 52-53 0.2278584119228313 2201 54-55 0.20400483053282414 2201 56-57 0.2518091392016615 2201 58-59 0.27061260528832776 2201 60-61 0.2942866296385773 2201 62-63 0.31398438652080785 2201 64-65 0.40350184103117925 2201 66-67 0.41887737563068583 2201 68-69 0.38895700304463077 2201 70-71 0.39172475590827815 2201 72-73 0.44403259076955237 2201 74-75 0.42389721780739364 2201 76-77 0.4630365277707398 2201 78-79 0.47600576964954655 2201 80-81 0.5188235680477007 2201 82-83 0.5109296533668584 2201 84-85 0.5400947255031738 2201 86-87 0.4884836040993292 2201 88-89 0.4938890680968768 2201 90-91 0.4808524817353046 2201 92-93 0.4975585333948658 2201 94-95 0.47586569393965306 2201 96-97 0.4329613176561864 2201 98-99 0.5163142061831323 2201 100-101 0.4558256568877468 1101 1 -0.4394993246387102 1101 2 -0.4115988099741692 1101 3 -0.7826594020256046 1101 4 -0.16214297995293947 1101 5 0.5807177693430887 1101 6 0.29280690177596114 1101 7 17.734440357276185 1101 8 11.06855032559567 1101 9 2.743729737538608 1101 10-11 0.27324584676517105 1101 12-13 0.01686662314303078 1101 14-15 0.07126522934726864 1101 16-17 0.026822448286303313 1101 18-19 0.013396960712114492 1101 20-21 0.01934237328780597 1101 22-23 0.017700255807888254 1101 24-25 0.026376170975723312 1101 26-27 0.040201836936653024 1101 28-29 0.033790640614029144 1101 30-31 0.03596846938203413 1101 32-33 5.222620852975979E-4 1101 34-35 0.03558890966202455 1101 36-37 0.009369111212421899 1101 38-39 0.022327241329072933 1101 40-41 0.01865252800112671 1101 42-43 0.03128627401886064 1101 44-45 0.06059239175422704 1101 46-47 0.06103563276710133 1101 48-49 0.046126008344742786 1101 50-51 0.10412541372610917 1101 52-53 0.01251284083573978 1101 54-55 0.05847432177758094 1101 56-57 0.09319927777345782 1101 58-59 0.1094805068502609 1101 60-61 0.12375509924017081 1101 62-63 0.12476945096953074 1101 64-65 0.18664619173720354 1101 66-67 0.17145804740972892 1101 68-69 0.15820742509077235 1101 70-71 0.17557430006793595 1101 72-73 0.24563110658471032 1101 74-75 0.1939754747872513 1101 76-77 0.186241464906459 1101 78-79 0.22633912288478086 1101 80-81 0.23832778693837753 1101 82-83 0.23017077268669084 1101 84-85 0.23408824406997297 1101 86-87 0.2104990827901574 1101 88-89 0.20655521468226823 1101 90-91 0.21129517357080374 1101 92-93 0.20578551933817835 1101 94-95 0.20094595996427245 1101 96-97 0.25100485859688604 1101 98-99 0.22961729400095976 1101 100-101 0.144430105807281 2202 1 -0.42766928238887303 2202 2 -0.48057390203605266 2202 3 -1.0945488937809067 2202 4 -0.6057093133438585 2202 5 -0.1573361350656839 2202 6 0.1334078355811883 2202 7 17.682975055472447 2202 8 11.118044330954742 2202 9 2.8650549152704983 2202 10-11 0.30198655081242265 2202 12-13 0.030978826923018232 2202 14-15 0.10097353539995169 2202 16-17 0.05886191779087824 2202 18-19 0.06651765555308486 2202 20-21 0.0751295028071155 2202 22-23 0.05916005775169708 2202 24-25 0.05529508639146741 2202 26-27 0.06896904525853387 2202 28-29 0.05969055725192618 2202 30-31 0.04311801268048754 2202 32-33 0.04440943438628864 2202 34-35 0.04637306632555038 2202 36-37 0.07170898976734463 2202 38-39 0.0671749509957138 2202 40-41 0.05673688893966755 2202 42-43 0.04704216098826208 2202 44-45 0.03294445291419379 2202 46-47 0.039280489511696715 2202 48-49 0.08512426316569588 2202 50-51 0.0899444772575606 2202 52-53 0.113779125406829 2202 54-55 0.12665174190524908 2202 56-57 0.1258392977557108 2202 58-59 0.16892119897489977 2202 60-61 0.18815263684360417 2202 62-63 0.18888475351536016 2202 64-65 0.22863340587668546 2202 66-67 0.2309226061874341 2202 68-69 0.22896814623810968 2202 70-71 0.24726855605187836 2202 72-73 0.2940792531445595 2202 74-75 0.2920105304998657 2202 76-77 0.30920277931431883 2202 78-79 0.33262011996023233 2202 80-81 0.3588982452581675 2202 82-83 0.36962366000582136 2202 84-85 0.3756666710080552 2202 86-87 0.3151139285095823 2202 88-89 0.3163941595893789 2202 90-91 0.34115671509421475 2202 92-93 0.3584498060843373 2202 94-95 0.37914014795486395 2202 96-97 0.4091880068584075 2202 98-99 0.38079721531688193 2202 100-101 0.3704861468267424 1102 1 0.26719149024297906 1102 2 0.3566722162879685 1102 3 1.650234697365228 1102 4 1.2369040849946629 1102 5 0.9504091425506118 1102 6 0.5917485508718556 1102 7 -16.72552629378864 1102 8 -10.453426330149707 1102 9 -2.211203142617709 1102 10-11 -0.05019675856519967 1102 12-13 0.09517522501538167 1102 14-15 0.1811674427074692 1102 16-17 0.203962869791809 1102 18-19 0.20771170378995407 1102 20-21 0.20707571549441184 1102 22-23 0.2378156021266875 1102 24-25 0.23358542826563422 1102 26-27 0.2555503904137666 1102 28-29 0.2559282203205768 1102 30-31 0.24029117806544065 1102 32-33 0.2518741719402371 1102 34-35 0.26386360256248764 1102 36-37 0.26558645666107594 1102 38-39 0.26718770011968473 1102 40-41 0.26730606547970126 1102 42-43 0.2745043087256889 1102 44-45 0.2466994643105238 1102 46-47 0.24779607945385607 1102 48-49 0.23508978968941108 1102 50-51 0.2556432164920466 1102 52-53 0.2496564202696092 1102 54-55 0.26693001048994347 1102 56-57 0.2144096752117477 1102 58-59 0.21736010292375596 1102 60-61 0.19953586927881872 1102 62-63 0.15411283515413743 1102 64-65 0.17575248444219227 1102 66-67 0.1481087258472087 1102 68-69 0.1723397224807499 1102 70-71 0.12935985871855848 1102 72-73 0.14008480373441046 1102 74-75 0.1435563484442497 1102 76-77 0.1285695045336439 1102 78-79 0.1680340079369529 1102 80-81 0.16904888056618717 1102 82-83 0.21743260001436227 1102 84-85 0.2197796730708106 1102 86-87 0.18345709490815665 1102 88-89 0.1881328726262268 1102 90-91 0.20585588439826097 1102 92-93 0.23278111855181294 1102 94-95 0.2727239092535356 1102 96-97 0.30049803377777806 1102 98-99 0.2993723727003257 1102 100-101 0.2972904190388306 2203 1 -0.9750454953792023 2203 2 -0.5163299316102865 2203 3 0.9754742074423604 2203 4 0.8215673028485426 2203 5 0.6430811378782977 2203 6 0.5071007232579987 2203 7 -16.72552629378864 2203 8 -10.453426330149707 2203 9 -2.314383724264843 2203 10-11 -0.07079556689826205 2203 12-13 0.08894070857223113 2203 14-15 0.16046936563319747 2203 16-17 0.19646432022199178 2203 18-19 0.18335412583893884 2203 20-21 0.21319351422533117 2203 22-23 0.2223276261429845 2203 24-25 0.22946821253883343 2203 26-27 0.22060081717262392 2203 28-29 0.23455178269792043 2203 30-31 0.24628412002757472 2203 32-33 0.24687284393890963 2203 34-35 0.2812724759297609 2203 36-37 0.24314196377788022 2203 38-39 0.2740333098404548 2203 40-41 0.26609426185049045 2203 42-43 0.2735419385076696 2203 44-45 0.2780618671090451 2203 46-47 0.27119217901776693 2203 48-49 0.2618920351694527 2203 50-51 0.2710631743167795 2203 52-53 0.2613572028229143 2203 54-55 0.290140842294349 2203 56-57 0.25528187495589094 2203 58-59 0.20490360404382812 2203 60-61 0.21488098190850735 2203 62-63 0.17290728043642645 2203 64-65 0.18984213264617011 2203 66-67 0.16995921074324372 2203 68-69 0.19775948557148126 2203 70-71 0.15975547941852142 2203 72-73 0.1657332055800822 2203 74-75 0.15426459200404707 2203 76-77 0.16057336375868658 2203 78-79 0.1699351174921162 2203 80-81 0.2228436452805127 2203 82-83 0.2493352359900456 2203 84-85 0.25185345768890244 2203 86-87 0.24929747423055204 2203 88-89 0.20895646808645552 2203 90-91 0.2606586117677949 2203 92-93 0.25777007167789634 2203 94-95 0.27173766782644293 2203 96-97 0.361720245434455 2203 98-99 0.28389018658146625 2203 100-101 0.28084583411762765 1103 1 3.0876996340521714 1103 2 1.202610617186945 1103 3 1.7046097059783278 1103 4 1.1752709265587669 1103 5 0.8772327665334032 1103 6 0.5617051188555209 1103 7 -16.72552629378864 1103 8 -10.453426330149707 1103 9 -2.5494893600017754 1103 10-11 -0.2039827086785948 1103 12-13 0.08421021745080282 1103 14-15 0.15130829500377274 1103 16-17 0.21164532302973527 1103 18-19 0.22253825398995986 1103 20-21 0.22856241093968777 1103 22-23 0.24027370975360185 1103 24-25 0.23222228089581165 1103 26-27 0.21800971790967338 1103 28-29 0.21580990328858007 1103 30-31 0.24900229469908197 1103 32-33 0.26751821544920773 1103 34-35 0.2713906743354144 1103 36-37 0.2688645987204552 1103 38-39 0.23067769381614056 1103 40-41 0.2768835044918063 1103 42-43 0.23926931697464937 1103 44-45 0.24079270056773794 1103 46-47 0.2475724654503182 1103 48-49 0.262656331621983 1103 50-51 0.2777781633730001 1103 52-53 0.2474339677968871 1103 54-55 0.2606497187801722 1103 56-57 0.2470573362662165 1103 58-59 0.2520206084611729 1103 60-61 0.25115816629910626 1103 62-63 0.2088535262261999 1103 64-65 0.20346120800682144 1103 66-67 0.23009266282574714 1103 68-69 0.27490033531973523 1103 70-71 0.23379282232927068 1103 72-73 0.2241256517667054 1103 74-75 0.2932428466397141 1103 76-77 0.32803225524848756 1103 78-79 0.38204323981396726 1103 80-81 0.4107912322203049 1103 82-83 0.45519422041776636 1103 84-85 0.4603127093703705 1103 86-87 0.47178882895079965 1103 88-89 0.4502429611578833 1103 90-91 0.4860133264692621 1103 92-93 0.5299343816278252 1103 94-95 0.549794689211943 1103 96-97 0.5645643152393518 1103 98-99 0.52228659116291 1103 100-101 0.4937266783712744 2204 1 -0.5175793868197438 2204 2 -0.6748612815594441 2204 3 1.236672709729124 2204 4 1.0650797562526932 2204 5 0.6672580874874114 2204 6 0.34802084871299144 2204 7 17.82156219886962 2204 8 11.140461463473148 2204 9 2.8583630463149063 2204 10-11 0.29505102654234605 2204 12-13 -0.002878933588689847 2204 14-15 0.0053921293039636 2204 16-17 -0.023754079372643844 2204 18-19 -0.05187608736625293 2204 20-21 -0.01171742421750821 2204 22-23 -0.04496154686157183 2204 24-25 -0.024683159920364517 2204 26-27 -0.047892971500807846 2204 28-29 -0.06827384503800005 2204 30-31 -0.011712194567579104 2204 32-33 -0.008043325648820598 2204 34-35 -0.03501024874844205 2204 36-37 -0.04007396596547608 2204 38-39 -0.0401644364019873 2204 40-41 -0.014897805399485264 2204 42-43 -0.035400775242500515 2204 44-45 -0.03548313555030802 2204 46-47 -0.04620001273472241 2204 48-49 -0.042894709219048366 2204 50-51 0.016334641958891893 2204 52-53 0.0029082190630944638 2204 54-55 0.014805321599126842 2204 56-57 0.02609754391614416 2204 58-59 0.011858687420918557 2204 60-61 0.03162889746641895 2204 62-63 0.027270351702149753 2204 64-65 0.12719479192558225 2204 66-67 0.13516138085754648 2204 68-69 0.16286423729086863 2204 70-71 0.13817479229211926 2204 72-73 0.18264590295459726 2204 74-75 0.14156465053225276 2204 76-77 0.2208795371652883 2204 78-79 0.24834661337002473 2204 80-81 0.2520505568855498 2204 82-83 0.29081148845254745 2204 84-85 0.2397868816515043 2204 86-87 0.21765588231775013 2204 88-89 0.1789574278434003 2204 90-91 0.2598556515104633 2204 92-93 0.24381075546751418 2204 94-95 0.25924470462733495 2204 96-97 0.3252970089224618 2204 98-99 0.2477971702341577 2204 100-101 0.27666324136099263 2205 1 -0.6423665714315874 2205 2 -0.4288091571866346 2205 3 1.0681342900382447 2205 4 0.8623226040726308 2205 5 0.7685064267344046 2205 6 0.5130551773419256 2205 7 -16.72552629378864 2205 8 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-0.10288473784399343 1215 96-97 -0.0835814659331362 1215 98-99 -0.10208093564828147 1215 100-101 -0.10938577668581217 1212 1 -2.5581234231661725 1212 2 -0.8752388146967469 1212 3 -2.8212388905163586 1212 4 -5.8477407093991545 1212 5 -6.400851945277175 1212 6 -5.866127455382223 1212 7 -16.72552629378864 1212 8 -10.453426330149707 1212 9 -4.1400864648944875 1212 10-11 -0.6767761729417359 1212 12-13 -0.17319436964755397 1212 14-15 -0.28714353422521555 1212 16-17 -0.23677709080274667 1212 18-19 -0.19995904667302256 1212 20-21 -0.21716510978458103 1212 22-23 -0.20107719926261325 1212 24-25 -0.2212493327352263 1212 26-27 -0.26622471388370883 1212 28-29 -0.2955349648831742 1212 30-31 -0.2903250002297071 1212 32-33 -0.3141197464166581 1212 34-35 -0.4136434022760298 1212 36-37 -0.3758106476380121 1212 38-39 -0.30806071591452877 1212 40-41 -0.3752794360211311 1212 42-43 -0.295411961915903 1212 44-45 -0.35834922656380286 1212 46-47 -0.3617240067213672 1212 48-49 -0.3699691817967832 1212 50-51 -0.37641836000989315 1212 52-53 -0.479908011668293 1212 54-55 -0.4657359203845317 1212 56-57 -0.45455430089882753 1212 58-59 -0.5641038017294662 1212 60-61 -0.6950574443773476 1212 62-63 -0.5987724565210044 1212 64-65 -0.6470994511072448 1212 66-67 -0.6563381721906438 1212 68-69 -0.7102297115393199 1212 70-71 -0.7604416330823796 1212 72-73 -0.8696892200269559 1212 74-75 -0.9500738108441169 1212 76-77 -1.1071963784358232 1212 78-79 -1.254176406167339 1212 80-81 -1.2495948926095295 1212 82-83 -1.2587626932425877 1212 84-85 -1.280934107734204 1212 86-87 -1.3186368956593384 1212 88-89 -1.3230427064709147 1212 90-91 -1.3104195070064577 1212 92-93 -1.3260943163460652 1212 94-95 -1.2986649100274832 1212 96-97 -1.3185070723463141 1212 98-99 -1.339187914912678 1212 100-101 -1.1866206251612965 1213 1 -5.952661038726127 1213 2 -3.5046282994642404 1213 3 -5.210020231995294 1213 4 -3.1572528988769797 1213 5 -7.764006515029681 1213 6 -4.192684202694984 1213 7 -16.72552629378864 1213 8 -10.453426330149707 1213 9 -3.6232264558695313 1213 10-11 -0.5777195702597098 1213 12-13 -0.11373667702834922 1213 14-15 -0.23393292006529265 1213 16-17 -0.23294081857299886 1213 18-19 -0.2648387862220005 1213 20-21 -0.30129099058571995 1213 22-23 -0.25725223715586765 1213 24-25 -0.23552512328679853 1213 26-27 -0.25771655092538026 1213 28-29 -0.3671094294602213 1213 30-31 -0.3171317851464579 1213 32-33 -0.31248595431464565 1213 34-35 -0.34200422991091983 1213 36-37 -0.31010061200100125 1213 38-39 -0.35620034531275024 1213 40-41 -0.39579834215000886 1213 42-43 -0.4108577572113532 1213 44-45 -0.4026113350484266 1213 46-47 -0.4025699518542538 1213 48-49 -0.46103486439596963 1213 50-51 -0.5107433394723344 1213 52-53 -0.5911232858302995 1213 54-55 -0.5538427764595966 1213 56-57 -0.5831067063971105 1213 58-59 -0.6554884674010779 1213 60-61 -0.7509650889791502 1213 62-63 -0.7360753826899114 1213 64-65 -0.825001611311805 1213 66-67 -0.952251934672276 1213 68-69 -0.9983430904602599 1213 70-71 -1.0433213032086144 1213 72-73 -1.0982938950706398 1213 74-75 -1.1807561207704111 1213 76-77 -1.4033338557287962 1213 78-79 -1.4790586478279266 1213 80-81 -1.5268749237340629 1213 82-83 -1.611615274033312 1213 84-85 -1.610009613652494 1213 86-87 -1.6607806991003926 1213 88-89 -1.6748092985081229 1213 90-91 -1.7335408695436314 1213 92-93 -1.8110068436812732 1213 94-95 -1.8070902718935642 1213 96-97 -1.8578130328679094 1213 98-99 -2.005093115255164 1213 100-101 -1.8757355429531941 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 28.0 9 205.0 10 427.0 11 353.0 12 272.0 13 557.0 14 1887.0 15 5576.0 16 13414.0 17 27007.0 18 48001.0 19 79734.0 20 123638.0 21 173812.0 22 230771.0 23 315592.0 24 400231.0 25 468714.0 26 555573.0 27 684630.0 28 876167.0 29 1135581.0 30 1467812.0 31 1952052.0 32 2703887.0 33 3873584.0 34 6008017.0 35 1.041594E7 36 1.9881081E7 37 3.2950676E7 38 1.0778858E7 39 16715.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.62205882275682 22.082927446889773 32.3335023249925 22.961511405360906 2 23.028026207032937 7.949167804543464 19.25505220029506 49.76775378812854 3 16.032402125124236 34.621285888627526 26.418530303442555 22.92778168280568 4 19.537280044118553 23.03529477861063 22.44699524199788 34.980429935272944 5 33.90662731410916 24.195714850275973 22.603113750784395 19.294544084830477 6 25.430681337558752 33.96544703617878 33.07132950529757 7.532542120964894 7 0.5255680844784265 0.584753519914749 98.2579266461353 0.6317517494715332 8 41.86801299293711 12.81306887722777 9.310118791529359 36.008799338305764 9 26.13689302770182 10.614872064204024 27.304040556695007 35.94419435139915 10-11 27.913461883719553 13.875152674953 17.285111100134326 40.92627434119312 12-13 27.09398069353388 23.516961941433472 23.932986776849262 25.456070588183387 14-15 21.192185349352165 23.86510926676376 34.27178787898334 20.67091750490074 16-17 20.133143862630245 27.64241729089895 31.414835661524158 20.809603184946646 18-19 23.248852223502105 27.943445592643197 26.60140413598424 22.206298047870458 20-21 21.61866616020261 25.732615446654417 28.856685963755467 23.792032429387504 22-23 20.472153353682888 29.858571988065336 27.832644412155783 21.836630246095996 24-25 21.323539963494635 28.76236044938581 29.17875757419705 20.7353420129225 26-27 21.076659237715166 28.244423904348455 29.768806155568733 20.91011070236765 28-29 21.718336361696146 30.060441537767108 27.712058980821748 20.509163119714994 30-31 21.462471252730968 28.01528508454029 28.7679767658176 21.754266896911137 32-33 21.700828415234813 27.819323192776903 29.146608734637518 21.333239657350767 34-35 21.845306604576344 27.875143554452364 29.235627431181054 21.043922409790238 36-37 22.916918344889314 25.693805522378256 29.814507466110037 21.574768666622393 38-39 21.066291261344663 28.793978327077564 26.68628911754108 23.453441294036697 40-41 20.60972629854761 29.82602165046163 27.022753196797417 22.541498854193346 42-43 21.995279816659583 27.10501605859071 26.556270767108003 24.343433357641704 44-45 22.022995334625158 28.293395460242415 26.757227187746462 22.926382017385965 46-47 20.25947316160454 29.82925185208814 27.772178623170134 22.139096363137185 48-49 20.73107802776238 27.669199209640606 27.040807981299082 24.558914781297933 50-51 20.619094530354612 28.724719531129374 26.487719745288775 24.168466193227246 52-53 19.869446187785446 28.192976576535468 28.38215665117 23.555420584509093 54-55 20.905941282515197 25.764297123102054 29.225705376509413 24.10405621787334 56-57 21.401556436224283 25.655356441296203 30.129514940278785 22.81357218220073 58-59 22.343591860364146 26.698370117597715 26.329790357668408 24.62824766436973 60-61 23.90681130362249 25.591090247655675 28.526709736237727 21.975388712484108 62-63 25.65110497975256 24.663071410035723 27.539373187705525 22.14645042250619 64-65 22.076347004732412 26.1646898333467 27.84558767311049 23.9133754888104 66-67 22.187075674565758 29.310375854202874 26.63632052486084 21.86622794637053 68-69 23.112051122987413 28.932350833906312 25.73325092547455 22.222347117631728 70-71 21.897122184673652 28.260033258471395 25.736846796672598 24.105997760182355 72-73 24.315323124243765 24.539418928163727 26.864432919411335 24.280825028181173 74-75 21.18492509638468 26.476908575566764 24.855322978410083 27.48284334963847 76-77 23.59758089420696 27.203386008122727 24.625622539852028 24.573410557818285 78-79 23.42207086515325 28.93055567957567 25.301868933555976 22.345504521715107 80-81 25.59146213235704 26.44648494055003 25.272165499533493 22.689887427559434 82-83 25.775454644447127 27.584261004083626 26.22408367221548 20.416200679253762 84-85 23.845954792407237 24.326202047504456 29.103631200749714 22.724211959338593 86-87 22.213912303326307 27.55885668943995 26.20610035041834 24.021130656815405 88-89 22.090310014642803 28.474863336049072 25.86459043507926 23.570236214228867 90-91 22.23013482141767 26.495930871874805 27.93755809787309 23.336376208834434 92-93 23.341267241353545 27.336337415865746 25.967488126884874 23.35490721589583 94-95 21.983090087472114 29.94706189760325 25.389499324903202 22.680348690021432 96-97 23.213831476182456 29.693377176788754 25.427933713842116 21.66485763318667 98-99 23.120419606963253 28.1694924196136 25.511219078601233 23.19886889482191 100-101 22.85488908237402 28.223711211013647 27.803977269772677 21.117422436839654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 141.0 1 281.0 2 4280.0 3 22381.0 4 28507.5 5 16206.0 6 9682.5 7 6668.0 8 5390.5 9 4325.5 10 3793.0 11 3666.0 12 3627.5 13 3676.0 14 3963.5 15 4684.0 16 6041.5 17 10440.0 18 34512.0 19 88394.0 20 85880.0 21 57539.5 22 66650.0 23 77858.0 24 104890.0 25 129693.5 26 151620.0 27 201917.0 28 256092.5 29 324352.0 30 469821.0 31 571653.5 32 635350.0 33 740079.5 34 897010.5 35 1113469.0 36 1304968.5 37 1567343.5 38 1992734.0 39 2483472.5 40 2901228.5 41 3191016.0 42 3693298.0 43 4576030.5 44 5505044.0 45 6518665.0 46 7283851.5 47 7739472.5 48 6872640.5 49 5106226.0 50 4223368.5 51 3762329.5 52 3342510.5 53 3010901.5 54 2571626.0 55 2069793.5 56 1773933.0 57 1797634.0 58 1600887.0 59 1127057.0 60 773288.5 61 519042.0 62 403010.0 63 284723.0 64 216543.0 65 173306.0 66 142861.5 67 117705.5 68 95812.0 69 75917.5 70 58100.0 71 44243.5 72 34174.5 73 25611.0 74 18554.5 75 13566.5 76 9651.0 77 6655.5 78 4645.0 79 3143.0 80 2126.0 81 1510.5 82 1078.0 83 806.5 84 579.0 85 421.0 86 302.5 87 195.5 88 144.5 89 106.5 90 66.5 91 64.5 92 58.0 93 55.0 94 80.5 95 127.5 96 132.0 97 95.0 98 144.5 99 706.5 100 773.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.019375823254505053 2 0.004981574163568801 3 1.3026469765553168E-4 4 0.0 5 0.004659063984695831 6 0.001947667334301256 7 46.65160162441759 8 0.0 9 0.0 10-11 0.0 12-13 9.706821018847684E-4 14-15 0.0 16-17 0.0 18-19 4.632800940813667E-4 20-21 1.0190061026279495E-4 22-23 1.8384130717514553E-4 24-25 0.0015715805459086728 26-27 8.661551872337571E-4 28-29 0.004831349552562824 30-31 0.004352836893029803 32-33 0.0047604393340809825 34-35 4.5592644179436086E-4 36-37 6.602529231975941E-4 38-39 1.7911395927635608E-4 40-41 2.2481165563132083E-4 42-43 0.0 44-45 5.305134864197057E-5 46-47 3.492984836327765E-4 48-49 1.8384130717514553E-4 50-51 1.6808348084584735E-5 52-53 8.404174042292367E-6 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.6243000557385835E-5 70-71 9.454695797578912E-6 72-73 3.5192478802099285E-5 74-75 2.4004422108297573E-4 76-77 5.25260877643273E-7 78-79 1.512751327612626E-4 80-81 0.0 82-83 7.878913164649094E-6 84-85 3.834404406795893E-5 86-87 0.0 88-89 0.0 90-91 1.3866887169782405E-4 92-93 5.25260877643273E-7 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.785886983987128E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9.5190794E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.968764331565342 #Duplication Level Percentage of deduplicated Percentage of total 1 64.14807420111494 12.809577760458307 2 16.37590697362509 6.540132541439135 3 5.37069836299803 3.217386297198942 4 2.925605936838206 2.336829419190023 5 2.0112689051591164 2.0081277387263925 6 1.1664363189082083 1.3975375176033962 7 0.8402114036803099 1.1744588456150074 8 0.6175856543929548 0.986593790970278 9 0.5620631726336307 1.010133633039561 >10 4.957202112202261 21.06622032004698 >50 0.5824929277762145 7.952515469776536 >100 0.36892261639315316 14.60202804565321 >500 0.04310284282897245 5.919845973404799 >1k 0.027194872136095803 10.65995719043505 >5k 0.0021496864379279886 2.8741362002852533 >10k+ 0.0010840128752461573 5.444519256157189 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 296419 0.31139460818028264 No Hit TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 196812 0.20675528770145568 No Hit CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 174327 0.18313430603383768 No Hit ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 143984 0.15125832441317802 No Hit TCGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 131578 0.13822555151709312 No Hit TGACCATGCCCACAGTAACGGACAGCTTTACCTGTTTCTATGGAGACAAC 102843 0.10803880887893424 No Hit GTGGCCTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 101708 0.106846466686684 No Hit GCCAATTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 101467 0.10659329094365995 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.050521755286546E-5 0.0 0.0 2 1.050521755286546E-5 0.0 0.0 3 1.050521755286546E-5 0.0 0.0 4 1.4076991520839714E-4 0.0 0.0 5 1.4707304574011643E-4 0.0 0.0 6 1.7228556786699352E-4 1.050521755286546E-6 0.0 7 1.94346524728011E-4 1.050521755286546E-6 0.0 8 0.0021661758594008576 1.050521755286546E-6 0.0 9 0.002264924904397793 1.050521755286546E-6 2.101043510573092E-6 10-11 0.0027586701293824695 1.050521755286546E-6 2.101043510573092E-6 12-13 0.01753583440012067 1.050521755286546E-6 2.101043510573092E-6 14-15 0.2882673717376494 1.050521755286546E-6 3.1515652658596378E-6 16-17 0.2884370310011281 1.050521755286546E-6 4.202087021146184E-6 18-19 0.2885651946552731 3.1515652658596378E-6 4.202087021146184E-6 20-21 0.2887148940054014 3.1515652658596378E-6 4.202087021146184E-6 22-23 0.2888152188330313 3.1515652658596378E-6 4.202087021146184E-6 24-25 0.2888850785297578 3.6768261435029107E-6 4.202087021146184E-6 26-27 0.2889612413570161 4.202087021146184E-6 4.884926162082438E-5 28-29 0.28902584844496626 4.202087021146184E-6 9.034487095464295E-5 30-31 0.2890852029241399 4.202087021146184E-6 9.034487095464295E-5 32-33 0.2893079135362607 6.3031305317192756E-6 9.034487095464295E-5 34-35 0.29006271341743406 6.3031305317192756E-6 9.244591446521603E-5 36-37 0.29170993152972335 6.3031305317192756E-6 9.454695797578913E-5 38-39 0.2943677515705983 6.3031305317192756E-6 9.50722188534324E-5 40-41 0.2994149833438725 6.3031305317192756E-6 9.559747973107568E-5 42-43 0.3088523455324892 6.3031305317192756E-6 9.612274060871895E-5 44-45 0.32410382037573926 6.3031305317192756E-6 9.664800148636222E-5 46-47 0.34301846457967355 6.3031305317192756E-6 9.769852324164877E-5 48-49 0.37023538221563734 6.3031305317192756E-6 9.769852324164877E-5 50-51 0.4120508754239407 6.3031305317192756E-6 1.0032482762986514E-4 52-53 0.4625174152870287 6.3031305317192756E-6 1.0085008850750841E-4 54-55 0.5296347249714085 9.454695797578912E-6 1.0085008850750841E-4 56-57 0.6043304985984255 9.454695797578912E-6 1.0085008850750841E-4 58-59 0.6922019160802462 9.454695797578912E-6 1.0085008850750841E-4 60-61 0.7969000657773693 1.1555739308152004E-5 1.0400165377336804E-4 62-63 0.9257990851510283 1.2606261063438551E-5 1.0610269728394114E-4 64-65 1.0818393845942706 1.2606261063438551E-5 1.0610269728394114E-4 66-67 1.2692529909982682 1.2606261063438551E-5 1.0610269728394114E-4 68-69 1.4722384813808782 1.2606261063438551E-5 1.0610269728394114E-4 70-71 1.6993103345686977 1.2606261063438551E-5 1.0610269728394114E-4 72-73 1.9689818954551424 1.2606261063438551E-5 1.0820374079451423E-4 74-75 2.2878882594466017 1.2606261063438551E-5 1.0925426254980077E-4 76-77 2.6537251070728543 1.2606261063438551E-5 1.0925426254980077E-4 78-79 3.067372250303953 1.2606261063438551E-5 1.0925426254980077E-4 80-81 3.4792187992464902 1.2606261063438551E-5 1.0925426254980077E-4 82-83 3.9062811052925976 1.2606261063438551E-5 1.108300451827306E-4 84-85 4.375870633036216 1.3656782818725096E-5 1.1240582781566041E-4 86-87 4.882301433476855 1.3656782818725096E-5 1.1240582781566041E-4 88-89 5.421666616206605 1.3656782818725096E-5 1.1240582781566041E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCAGAT 577185 0.0 164.11485 1 GATCAGT 223300 0.0 158.39488 1 ACAGTGT 601975 0.0 158.24388 1 CGATGTT 593315 0.0 157.69719 1 GACCATG 268925 0.0 154.2353 2 CTCAGTC 160800 0.0 151.3942 2 CAGTGTG 301125 0.0 149.20279 2 TCGCTTT 320030 0.0 148.58879 1 TCAGATG 246660 0.0 147.5601 2 AGTGTGC 143065 0.0 147.42465 3 ACTTGAT 549040 0.0 147.35265 1 GTGGCCT 269520 0.0 147.08908 1 CTCAGTG 192865 0.0 145.97195 2 GCCAATT 400935 0.0 145.96242 1 TCTCAGT 215500 0.0 145.24171 1 GCTCAGT 245790 0.0 145.23862 1 TCAGTCG 64225 0.0 143.48045 3 TGACCAT 644640 0.0 137.83652 1 TGGCCTG 186785 0.0 137.58383 2 ATCAGTC 112925 0.0 137.37038 2 >>END_MODULE