FastQCFastQC Report
Wed 1 Oct 2014
Complete_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95190794
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA2964190.31139460818028264No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1968120.20675528770145568No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1743270.18313430603383768No Hit
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1439840.15125832441317802No Hit
TCGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1315780.13822555151709312No Hit
TGACCATGCCCACAGTAACGGACAGCTTTACCTGTTTCTATGGAGACAAC1028430.10803880887893424No Hit
GTGGCCTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1017080.106846466686684No Hit
GCCAATTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1014670.10659329094365995No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT5771850.0164.114851
GATCAGT2233000.0158.394881
ACAGTGT6019750.0158.243881
CGATGTT5933150.0157.697191
GACCATG2689250.0154.23532
CTCAGTC1608000.0151.39422
CAGTGTG3011250.0149.202792
TCGCTTT3200300.0148.588791
TCAGATG2466600.0147.56012
AGTGTGC1430650.0147.424653
ACTTGAT5490400.0147.352651
GTGGCCT2695200.0147.089081
CTCAGTG1928650.0145.971952
GCCAATT4009350.0145.962421
TCTCAGT2155000.0145.241711
GCTCAGT2457900.0145.238621
TCAGTCG642250.0143.480453
TGACCAT6446400.0137.836521
TGGCCTG1867850.0137.583832
ATCAGTC1129250.0137.370382
Produced by FastQC (version 0.11.2)