##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Complete_Undetermined_L002_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 128280483 Sequences flagged as poor quality 0 Sequence length 7 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.44543965429254 33.0 33.0 33.0 22.0 33.0 2 30.46800804452849 33.0 33.0 33.0 22.0 33.0 3 30.454056031267047 33.0 33.0 33.0 22.0 33.0 4 33.95107187115907 37.0 37.0 37.0 27.0 37.0 5 33.96926674340632 37.0 37.0 37.0 27.0 37.0 6 33.877343469310134 37.0 37.0 37.0 22.0 37.0 7 27.43976152631106 33.0 27.0 33.0 15.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.4705300882734349 2201 2 0.4346045324975911 2201 3 0.4734179267143652 2201 4 0.528963595535032 2201 5 0.5263817883553301 2201 6 0.5373689434262303 2201 7 2.2358805429559254 1101 1 0.455020293603031 1101 2 0.42723715305809407 1101 3 0.4473213492423973 1101 4 0.5194445920679982 1101 5 0.5215081982995216 1101 6 0.5296028481432202 1101 7 2.2106147962979534 2202 1 0.37577859243640077 2202 2 0.3630572759310873 2202 3 0.3861723574115068 2202 4 0.46683648805057487 2202 5 0.46196166220584445 2202 6 0.4644679224499342 2202 7 1.6101618749342919 1102 1 0.3893904467824534 1102 2 0.381623277175958 1102 3 0.3701327669773953 1102 4 0.4246928475517535 1102 5 0.43642260918724674 1102 6 0.446317885126156 1102 7 1.9781926879293152 2203 1 0.5403359976133224 2203 2 0.516595807402414 2203 3 0.5399849023106427 2203 4 0.6107209926652644 2203 5 0.6154315103753873 2203 6 0.6440268204934014 2203 7 2.2154976850141033 1103 1 0.3393772664156849 1103 2 0.31141663847878576 1103 3 0.3458434306714899 1103 4 0.41013289402837216 1103 5 0.3865165715559584 1103 6 0.38718508448187094 1103 7 1.7666737860756783 2204 1 0.31724406984600506 2204 2 0.2847107510148916 2204 3 0.332475188028333 2204 4 0.37005845960908346 2204 5 0.36306622082432227 2204 6 0.40355448876174194 2204 7 1.8657704858612618 2205 1 0.22159045093619767 2205 2 0.20968715500069735 2205 3 0.21157144519549576 2205 4 0.2567418086380471 2205 5 0.23401046464917385 2205 6 0.212562944365132 2205 7 1.5753308090273883 1216 1 0.23048941947102008 1216 2 0.23780276840012604 1216 3 0.27046038838066977 1216 4 0.29854928208831666 1216 5 0.28879041531381944 1216 6 0.2777290157745469 1216 7 1.3852082572578865 2206 1 0.3936632523423711 2206 2 0.37258919091415876 2206 3 0.3745467475994211 2206 4 0.4584952027467253 2206 5 0.4557531333949072 2206 6 0.4660358242935203 2206 7 1.3063944047193878 2207 1 0.2650528721901928 2207 2 0.24519204274439588 2207 3 0.2782135261526193 2207 4 0.32602425364429877 2207 5 0.3209408779249472 2207 6 0.316552574938207 2207 7 0.9448219119826398 1116 1 -0.00883066578581193 1116 2 -0.03287825579096193 1116 3 -0.014391866063029113 1116 4 -0.027077291464962627 1116 5 -0.0703657963839035 1116 6 -0.027693088457752424 1116 7 0.030977260729681433 1113 1 -0.009802932662733355 1113 2 -0.03656842248614822 1113 3 -0.027756834121596796 1113 4 -0.0019898037299768134 1113 5 -0.0277409613674493 1113 6 -0.002997891881079795 1113 7 0.18965498001592707 1112 1 -0.24301665896158298 1112 2 -0.268397260119805 1112 3 -0.23558183914191844 1112 4 -0.30427555800087447 1112 5 -0.2645036715688889 1112 6 -0.3341656030880671 1112 7 -0.45837115623542957 1115 1 -0.23767982153028555 1115 2 -0.2154515231749876 1115 3 -0.19895714829676336 1115 4 -0.3266322733101177 1115 5 -0.2699587867299371 1115 6 -0.2696931509260594 1115 7 -0.2460326533517012 1114 1 -0.9072128720729502 1114 2 -0.9018088210619268 1114 3 -0.9200800366915267 1114 4 -1.1520156704509787 1114 5 -1.1054874690903347 1114 6 -1.1103421607103527 1114 7 -1.3493109498543632 1109 1 0.2963549694543346 1109 2 0.30365725587452275 1109 3 0.29701011892802853 1109 4 0.3660643583889893 1109 5 0.37596443950848624 1109 6 0.3854185268786736 1109 7 1.8446789949344229 1108 1 0.3671174406769566 1108 2 0.3946129612471623 1108 3 0.4013219107505819 1108 4 0.4505879372777244 1108 5 0.4623942097752689 1108 6 0.4573911874440313 1108 7 2.1071301530472084 1111 1 0.4200999048877172 1111 2 0.39989684210616616 1111 3 0.43062177094148524 1111 4 0.47380300683069265 1111 5 0.4716408676199535 1111 6 0.5015900104126487 1111 7 2.0154968079920508 1110 1 0.5352101949254049 1110 2 0.525474947375919 1110 3 0.5343761664227564 1110 4 0.6089397692224949 1110 5 0.5846239398337403 1110 6 0.6171558878146897 1110 7 2.0925137716587123 1105 1 -0.018766219974068576 1105 2 -0.016905483193440318 1105 3 -0.002813971534475712 1105 4 -0.01652953068209939 1105 5 -0.021691860354899006 1105 6 -0.005006756247531996 1105 7 1.3527104315434961 1104 1 0.08172127210688274 1104 2 0.05213969922723649 1104 3 0.10129604249313928 1104 4 0.0688963143857606 1104 5 0.08780362763349814 1104 6 0.09138959037267824 1104 7 1.4300294075694175 1107 1 0.26097914162678393 1107 2 0.23930972235149994 1107 3 0.2598322392022645 1107 4 0.26522571572022 1107 5 0.2748490674160706 1107 6 0.2985763130597263 1107 7 1.7050659830099733 1106 1 0.3752273886011075 1106 2 0.37036669798165534 1106 3 0.39517592382101085 1106 4 0.46537047808939747 1106 5 0.4346278272315871 1106 6 0.4582184078586735 1106 7 1.5978272370884206 2103 1 0.21072785316241038 2103 2 0.20285515915867336 2103 3 0.2317327647182168 2103 4 0.23317117986132985 2103 5 0.23322906823874234 2103 6 0.2256799765840185 2103 7 0.3213097970046128 2102 1 -0.13415654421178402 2102 2 -0.12180045428425146 2102 3 -0.11211497800256964 2102 4 -0.13343616753762433 2102 5 -0.16805535436316177 2102 6 -0.1425414159808014 2102 7 0.8623967550974037 2101 1 0.03220351941530453 2101 2 0.04554046994407912 2101 3 0.05866341318917634 2101 4 0.06229680544015537 2101 5 0.04170765699218748 2101 6 0.04869212930827871 2101 7 0.9080276601241302 2107 1 0.04627013412306624 2107 2 0.03555466397364526 2107 3 0.06116415127803165 2107 4 0.054497268726478865 2107 5 0.03147603774549168 2107 6 0.03892374567444534 2107 7 0.4508288414141006 2106 1 0.12502275180925437 2106 2 0.06851919627282399 2106 3 0.10286951244436437 2106 4 0.08394326815032116 2106 5 0.06857524927579561 2106 6 0.10697373985682646 2106 7 0.2219267308421351 2105 1 -0.49775138048993384 2105 2 -0.46835352865926083 2105 3 -0.46499889840479014 2105 4 -0.6108698030497166 2105 5 -0.621983031587753 2105 6 -0.5843327587172382 2105 7 -0.5331116849718249 2104 1 -0.2478120705549145 2104 2 -0.2819648861866888 2104 3 -0.24247858305173153 2104 4 -0.3213080489238962 2104 5 -0.3568017717519467 2104 6 -0.34537838163310397 2104 7 -0.43393607013157975 2111 1 -1.1813305859319776 2111 2 -1.1768981885255165 2111 3 -1.2012864975136353 2111 4 -1.4978737626157184 2111 5 -1.4665268477077689 2111 6 -1.4757588032643696 2111 7 -1.387974215966242 2110 1 0.47468743134938407 2110 2 0.4564867494846112 2110 3 0.4944731202429935 2110 4 0.5916116912452267 2110 5 0.5709985598294907 2110 6 0.6062392609429281 2110 7 0.5988350581307813 2109 1 0.6852720765246758 2109 2 0.6660079547759281 2109 3 0.7068002963368549 2109 4 0.8092784295403561 2109 5 0.8114879691659382 2109 6 0.8086215930469933 2109 7 1.3811064901937549 2108 1 0.6467587497430145 2108 2 0.6355951461782219 2108 3 0.637834564145269 2108 4 0.7413561010606315 2108 5 0.7203386519689374 2108 6 0.7691273229500126 2108 7 0.6629744001874869 2216 1 0.40742707098665676 2216 2 0.3765391629726693 2216 3 0.3771883958362139 2216 4 0.46842292076195235 2216 5 0.3614986260393138 2216 6 0.42970110665022077 2216 7 0.8889599324046102 2211 1 0.4030730009043033 2211 2 0.40577472095380784 2211 3 0.44206704071861225 2211 4 0.5072719402560395 2211 5 0.5170361091505811 2211 6 0.5146303806369943 2211 7 1.1511103962038796 2210 1 0.5866165991375816 2210 2 0.5531851335165854 2210 3 0.5857598256835317 2210 4 0.7011069459898991 2210 5 0.6879485278893114 2210 6 0.7413500061229215 2210 7 0.9799690236255572 2209 1 0.5535255672547557 2209 2 0.5420995151779628 2209 3 0.5486176061831003 2209 4 0.6450751906065477 2209 5 0.6253399233432688 2209 6 0.6524270445961164 2209 7 0.9377119994731515 2208 1 0.0959641379477354 2208 2 0.076557539848217 2208 3 0.10770141204222838 2208 4 0.11482885679144061 2208 5 0.08003437822108594 2208 6 0.07479805740088352 2208 7 0.20575243501541252 2215 1 0.01771208305045846 2215 2 8.58945105711939E-4 2215 3 0.02814723405770536 2215 4 0.028670194386506864 2215 5 0.007347763773204008 2215 6 0.051896248767583586 2215 7 0.39585514760929286 2214 1 -0.3601464881704963 2214 2 -0.3551822962340516 2214 3 -0.3694194357236462 2214 4 -0.40585827021664755 2214 5 -0.4803320341317132 2214 6 -0.46449486166734033 2214 7 -1.0760251764760689 2213 1 -0.20219414763500154 2213 2 -0.21628515475039478 2213 3 -0.2252532853006599 2213 4 -0.22018292519819482 2213 5 -0.23712062562591996 2213 6 -0.2716596459521341 2213 7 -0.5493149518004081 2212 1 -0.09793377087933663 2212 2 -0.08644620121734548 2212 3 -0.050661822662782185 2212 4 -0.050395154446825075 2212 5 -0.06909261720170434 2212 6 -0.10796938759781227 2212 7 -0.5549089307213713 2116 1 -0.010260898584459 2116 2 -0.038896873672957355 2116 3 -0.07125206411420137 2116 4 -0.07677992312184045 2116 5 -0.09617796839279436 2116 6 -0.11489560717210878 2116 7 -0.7956298486283053 2112 1 -0.12103466022132636 2112 2 -0.12533798580701117 2112 3 -0.1472085567975192 2112 4 -0.1292072170572851 2112 5 -0.12625383319947048 2112 6 -0.16731369069090363 2112 7 -2.1510316557227327 2113 1 -0.23681779540060788 2113 2 -0.23610554278780072 2113 3 -0.2164158517404111 2113 4 -0.27300515022872673 2113 5 -0.3698996543968036 2113 6 -0.2719295824024144 2113 7 -1.5566151601129192 2114 1 -3.781649983471759 2114 2 -3.480187092673262 2114 3 -3.54716406052912 2114 4 -4.1027148502386055 2114 5 -3.91524057721789 2114 6 -4.273798765021464 2114 7 -4.0825406333949985 2115 1 0.5662181766787562 2115 2 0.5280625429959827 2115 3 0.5537951210418335 2115 4 0.6572087828189623 2115 5 0.6295807157393298 2115 6 0.6521778359839985 2115 7 0.656012150237963 1202 1 0.2262800409889003 1202 2 0.21872039753169759 1202 3 0.2008541906084318 1202 4 0.25552789735036185 1202 5 0.25303705359105777 1202 6 0.1695548991524447 1202 7 0.7889187520455998 1203 1 0.30466862046010235 1203 2 0.2989209236857242 1203 3 0.3245188165549244 1203 4 0.37423384179528085 1203 5 0.34747624252142373 1203 6 0.30719711203656885 1203 7 -0.22351621598307503 1201 1 0.4594124771241326 1201 2 0.4507133399710703 1201 3 0.4708371624647363 1201 4 0.5641095913481635 1201 5 0.5529268617891674 1201 6 0.5741616640940066 1201 7 0.8989817984121586 1206 1 0.39013307336892566 1206 2 0.3876858884586767 1206 3 0.39412041756546046 1206 4 0.4630560964969064 1206 5 0.4312020062431188 1206 6 0.45138208451870554 1206 7 0.5529297607781309 1207 1 0.15346391547354798 1207 2 0.17017742606139308 1207 3 0.17520375221477735 1207 4 0.18300799054691907 1207 5 0.1522568349038238 1207 6 0.16539262201943217 1207 7 0.09409075684939339 1204 1 0.28374335292798136 1204 2 0.26926340375756297 1204 3 0.24168214568365798 1204 4 0.335090152998184 1204 5 0.3030787534534838 1204 6 0.3250445035595959 1204 7 0.8708054248138168 1205 1 0.4367053086159487 1205 2 0.4142569073448783 1205 3 0.4092311790052712 1205 4 0.4957160151521123 1205 5 0.4801445521163501 1205 6 0.5006117835840556 1205 7 0.5254262532928884 1210 1 -0.46159397193493845 1210 2 -0.3606826159695693 1210 3 -1.2521277386185794 1210 4 -0.820644085230164 1210 5 -0.4742420876957425 1210 6 -0.3796839758142241 1210 7 -0.4141874039986959 1211 1 0.29404158575533046 1211 2 0.29864286996577505 1211 3 0.3254570396817691 1211 4 0.3756743723815319 1211 5 0.36783826505838135 1211 6 0.36701190589533894 1211 7 0.06853899540083219 1208 1 0.1637398299840349 1208 2 0.17323258291960286 1208 3 0.1975289128923663 1208 4 0.21830154463904705 1208 5 0.2669157204428956 1208 6 0.19988934923836155 1208 7 -0.6210943321399327 1209 1 0.09823798405120243 1209 2 0.09479956732220529 1209 3 0.10582139797675438 1209 4 0.0941653891685803 1209 5 0.10585089964450844 1209 6 0.09080137621648987 1209 7 -0.3071396312473489 1214 1 -0.01901963820504804 1214 2 -0.035818601448827536 1214 3 -0.016659354489576117 1214 4 -0.019932223741797372 1214 5 -0.04519871586082047 1214 6 -0.07847273905043295 1214 7 -1.1868693091191176 1215 1 -3.9773885920882535 1215 2 -3.6859958752190565 1215 3 -3.6303679706239365 1215 4 -4.33188537789114 1215 5 -4.147328989143109 1215 6 -4.313366462931111 1215 7 -25.52565920880523 1212 1 -0.2600522309879132 1212 2 -0.2737170564302289 1212 3 -0.27090392921774153 1212 4 -0.35712410406291895 1212 5 -0.355366410168628 1212 6 -0.34238836340192336 1212 7 -1.9693603951288807 1213 1 -0.9826364732714197 1213 2 -1.0243428064661373 1213 3 -1.0139489511688673 1213 4 -1.2474332728534492 1213 5 -1.260644794301058 1213 6 -1.28354693232383 1213 7 -2.4604712450117034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 278920.0 3 23288.0 4 1397.0 5 20265.0 6 64018.0 7 258032.0 8 543000.0 9 633750.0 10 421242.0 11 526537.0 12 716347.0 13 733823.0 14 395886.0 15 1911702.0 16 1461674.0 17 660818.0 18 1133235.0 19 943357.0 20 849055.0 21 1471780.0 22 1013188.0 23 864945.0 24 866097.0 25 1062554.0 26 1082023.0 27 1436493.0 28 1380578.0 29 1739802.0 30 3288338.0 31 3374673.0 32 1.0729548E7 33 3.2853721E7 34 5.0276426E7 35 5263971.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.612373475892568 25.46986608032492 28.538394042346084 20.37936640143643 2 27.45507586555008 25.730292062752998 27.13302411224519 19.681607959451732 3 27.78639189199974 26.056886285841795 27.133614824338682 19.023106997819784 4 27.70593228926363 26.335815522777562 26.437225550577796 19.521026637381013 5 27.366488565729153 26.88055578931971 25.887579575206644 19.865376069744492 6 27.416181473718364 28.01739609565452 25.82405893459739 18.742363496029725 7 1.0748088344740665 96.71945201727868 0.948328645743775 1.2574105025034703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3844094.0 1 3844094.0 2 3844094.0 3 3844094.0 4 3844094.0 5 3844094.0 6 3844094.0 7 1.0702426E7 8 1.7560758E7 9 1.7560758E7 10 1.7560758E7 11 1.7560758E7 12 1.7560758E7 13 1.7560758E7 14 1.7560758E7 15 1.7560758E7 16 1.7560758E7 17 1.7560758E7 18 1.7560758E7 19 1.7560758E7 20 1.7560758E7 21 2.55127645E7 22 3.3464771E7 23 3.3464771E7 24 3.3464771E7 25 3.3464771E7 26 3.3464771E7 27 3.3464771E7 28 3.3464771E7 29 3.3464771E7 30 3.3464771E7 31 3.3464771E7 32 3.3464771E7 33 3.3464771E7 34 3.3464771E7 35 3.3464771E7 36 3.410848E7 37 3.4752189E7 38 3.4752189E7 39 3.4752189E7 40 3.4752189E7 41 3.4752189E7 42 3.4752189E7 43 3.4752189E7 44 3.4752189E7 45 3.4752189E7 46 3.4752189E7 47 3.4752189E7 48 3.4752189E7 49 3.4752189E7 50 2.92626945E7 51 2.37732E7 52 2.37732E7 53 2.37732E7 54 2.37732E7 55 2.37732E7 56 2.37732E7 57 2.37732E7 58 2.37732E7 59 2.37732E7 60 2.37732E7 61 2.37732E7 62 2.37732E7 63 2.37732E7 64 1.7570054E7 65 1.1366908E7 66 1.1366908E7 67 1.1366908E7 68 1.1366908E7 69 1.1366908E7 70 1.1366908E7 71 1.1366908E7 72 1.1366908E7 73 1.1366908E7 74 1.1366908E7 75 1.1366908E7 76 1.1366908E7 77 1.1366908E7 78 1.1366908E7 79 7374174.0 80 3381440.0 81 3381440.0 82 3381440.0 83 3381440.0 84 3381440.0 85 3381440.0 86 3381440.0 87 3381440.0 88 3381440.0 89 3381440.0 90 3381440.0 91 3381440.0 92 3381440.0 93 1759281.5 94 137123.0 95 137123.0 96 137123.0 97 137123.0 98 137123.0 99 137123.0 100 137123.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24844231370722233 2 0.23637734510245026 3 0.27103109675694004 4 0.2677188236031197 5 0.23580594095518023 6 0.2611402702623126 7 1.8319926344524289 >>END_MODULE >>Sequence Length Distribution pass #Length Count 7 1.28280483E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 0.014898908083877603 #Duplication Level Percentage of deduplicated Percentage of total 1 15.219126803652241 0.002267483713644927 2 7.6175807150181125 0.00226987269789147 3 2.541867884552003 0.0011361316791990209 4 0.6361360181357611 3.791092825219441E-4 5 1.0188888542661363 7.590165693699262E-4 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 3.2006591424978477 0.01850594874050625 >50 8.591419118501044 0.09485163856405496 >100 30.54290943406751 1.1459654667459909 >500 6.11182288481611 0.6161264813841625 >1k 2.6072242481571535 0.7240180223791437 >5k 0.34171457750447154 0.3738843099196563 >10k+ 21.57065031883179 97.01983651832383 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGA 951866 0.742019345218711 No Hit NNNNNNN 277139 0.2160414378857616 No Hit TGGGGGA 272258 0.21223649430755573 No Hit GGGGAGA 218483 0.17031663343518905 No Hit GGGAGGA 210632 0.1641964506790951 No Hit GGGGGAA 183907 0.1433631957871565 No Hit AGGGGGA 182819 0.14251505429707495 No Hit CGGGGGA 182371 0.1421658195658649 No Hit GGGGGTA 173409 0.13517956585804247 No Hit GGGGTGA 170520 0.1329274695668241 No Hit GTGGGGA 166852 0.13006811020504186 No Hit GGAGGGA 159613 0.12442500703711881 No Hit GCGGGGA 157163 0.12251512960081386 No Hit GGGCGGA 151301 0.11794545550627526 No Hit GGGTGGA 145241 0.11322143213321079 No Hit GGGGCGA 144051 0.11229377737843409 No Hit GAGGGGA 137269 0.10700692481801773 No Hit GGTGGGA 135069 0.10529193283439695 No Hit >>END_MODULE >>Adapter Content warn >>END_MODULE >>Kmer Content pass >>END_MODULE