##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Complete_Undetermined_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 128280483 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.930943516949498 27.0 15.0 33.0 15.0 33.0 2 27.352321872688925 27.0 27.0 33.0 15.0 33.0 3 27.833420349687955 33.0 27.0 33.0 15.0 33.0 4 32.730686436532984 37.0 33.0 37.0 15.0 37.0 5 33.3143306141122 37.0 33.0 37.0 27.0 37.0 6 33.72814825619264 37.0 33.0 37.0 27.0 37.0 7 18.93343436351109 2.0 2.0 37.0 2.0 37.0 8 24.752962451817396 15.0 15.0 37.0 15.0 37.0 9 31.201201167912657 33.0 27.0 37.0 15.0 37.0 10-11 33.49717861601753 37.0 33.0 37.0 27.0 37.0 12-13 33.67397198293992 37.0 33.0 37.0 27.0 37.0 14-15 35.43915043958792 40.0 33.0 40.0 27.0 40.0 16-17 35.4263839768985 40.0 33.0 40.0 27.0 40.0 18-19 35.301409388987096 40.0 33.0 40.0 27.0 40.0 20-21 35.28277199034244 40.0 33.0 40.0 27.0 40.0 22-23 35.090540152549934 40.0 33.0 40.0 27.0 40.0 24-25 35.1862693485493 40.0 33.0 40.0 27.0 40.0 26-27 35.18712346912507 40.0 33.0 40.0 22.0 40.0 28-29 35.16491537921634 40.0 33.0 40.0 22.0 40.0 30-31 35.06714239608842 40.0 33.0 40.0 22.0 40.0 32-33 35.006293100720555 40.0 33.0 40.0 22.0 40.0 34-35 34.971571068998855 40.0 33.0 40.0 22.0 40.0 36-37 34.94837317146678 40.0 33.0 40.0 22.0 40.0 38-39 34.85840512854945 40.0 33.0 40.0 22.0 40.0 40-41 34.71790317081984 40.0 33.0 40.0 22.0 40.0 42-43 34.62669739480167 40.0 33.0 40.0 22.0 40.0 44-45 34.525289700538465 37.0 33.0 40.0 22.0 40.0 46-47 34.41381845280392 37.0 33.0 40.0 22.0 40.0 48-49 34.27613032139893 37.0 33.0 40.0 22.0 40.0 50-51 34.11892980243924 37.0 33.0 40.0 22.0 40.0 52-53 33.97668091879573 37.0 33.0 40.0 22.0 40.0 54-55 33.80735277166052 37.0 33.0 40.0 22.0 40.0 56-57 33.593769907305386 37.0 33.0 40.0 22.0 40.0 58-59 33.385966456799196 37.0 33.0 40.0 22.0 40.0 60-61 33.13268985742749 37.0 33.0 40.0 22.0 40.0 62-63 32.90872556583685 37.0 33.0 37.0 15.0 40.0 64-65 32.642108741514484 37.0 33.0 37.0 15.0 40.0 66-67 32.31655393751519 37.0 33.0 37.0 15.0 40.0 68-69 32.024731002922714 37.0 33.0 37.0 15.0 40.0 70-71 31.76494292978301 37.0 30.0 37.0 15.0 38.5 72-73 31.51732245192747 35.0 27.0 37.0 15.0 37.0 74-75 31.43763715794553 35.0 27.0 37.0 15.0 37.0 76-77 31.38911909148331 33.0 27.0 37.0 15.0 37.0 78-79 31.185113264657726 33.0 27.0 37.0 15.0 37.0 80-81 31.038278819857577 33.0 27.0 37.0 15.0 37.0 82-83 30.873182848087655 33.0 27.0 37.0 15.0 37.0 84-85 30.48325125966356 33.0 27.0 37.0 15.0 37.0 86-87 30.620509243795098 33.0 27.0 37.0 15.0 37.0 88-89 30.52180108723164 33.0 27.0 37.0 15.0 37.0 90-91 30.382244261584205 33.0 27.0 37.0 15.0 37.0 92-93 30.303610764390402 33.0 27.0 37.0 15.0 37.0 94-95 30.13359386478144 33.0 27.0 37.0 15.0 37.0 96-97 30.03800124060961 33.0 27.0 37.0 15.0 37.0 98-99 29.984897234912967 33.0 27.0 37.0 15.0 37.0 100-101 29.53351713292193 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 -1.487934724183404 2201 2 -0.43104791664174513 2201 3 0.876306487462724 2201 4 0.8347519599007143 2201 5 0.815261361545609 2201 6 0.7306048446065887 2201 7 -17.435493910512967 2201 8 -10.342088125511491 2201 9 -2.4218482349752044 2201 10-11 -0.05581898434363808 2201 12-13 0.35142434572237846 2201 14-15 0.3943737117609487 2201 16-17 0.4279385578597328 2201 18-19 0.4549749958373823 2201 20-21 0.4563074898010342 2201 22-23 0.41489502628978414 2201 24-25 0.44478667370474056 2201 26-27 0.41925591324184097 2201 28-29 0.4722404950298582 2201 30-31 0.44004618584509814 2201 32-33 0.4042290350736195 2201 34-35 0.4893528126513047 2201 36-37 0.5147001640506375 2201 38-39 0.46631263249705057 2201 40-41 0.4904695077047734 2201 42-43 0.49059439341316136 2201 44-45 0.48416281597290833 2201 46-47 0.5120426743896829 2201 48-49 0.4875658990702618 2201 50-51 0.49361494369225056 2201 52-53 0.4513711192646781 2201 54-55 0.4850573002476395 2201 56-57 0.48913224291358404 2201 58-59 0.4773124910555424 2201 60-61 0.4864991381865025 2201 62-63 0.41368119699640715 2201 64-65 0.3790917081491685 2201 66-67 0.3253731355385625 2201 68-69 0.38220492847677434 2201 70-71 0.4282280122573674 2201 72-73 0.3688728793379745 2201 74-75 0.294745749532499 2201 76-77 0.2739839760236471 2201 78-79 0.2866549385298818 2201 80-81 0.2982425288655044 2201 82-83 0.2890650712493965 2201 84-85 0.3247336862151755 2201 86-87 0.2792474785953303 2201 88-89 0.27969352510724477 2201 90-91 0.27587067097124063 2201 92-93 0.27752364828461396 2201 94-95 0.3101300893564449 2201 96-97 0.3082884196819009 2201 98-99 0.3180801550117742 2201 100-101 0.363815209466253 1101 1 0.05506050007781482 1101 2 0.31448697462641206 1101 3 1.5562789193926427 1101 4 1.3111368476126657 1101 5 1.0344695541606441 1101 6 0.8911371397138126 1101 7 -17.435493910512967 1101 8 -10.340186359104427 1101 9 -2.180130752195584 1101 10-11 0.0765636300938084 1101 12-13 0.40440551526015867 1101 14-15 0.45707977382262754 1101 16-17 0.4883040751367247 1101 18-19 0.4916157311658793 1101 20-21 0.4981770137794328 1101 22-23 0.542130000470614 1101 24-25 0.5100709815956606 1101 26-27 0.5539135105844082 1101 28-29 0.5366655919339536 1101 30-31 0.5751102113543141 1101 32-33 0.5563631977533632 1101 34-35 0.5545989195148877 1101 36-37 0.5452015120565932 1101 38-39 0.5447512127494392 1101 40-41 0.5531416662663133 1101 42-43 0.5810578620868867 1101 44-45 0.5571775357306095 1101 46-47 0.5717260040867203 1101 48-49 0.6251198370284996 1101 50-51 0.6057835095150494 1101 52-53 0.5506881940522703 1101 54-55 0.5711293710651404 1101 56-57 0.617340118336017 1101 58-59 0.4996021154113208 1101 60-61 0.5441116860964499 1101 62-63 0.5532955045423904 1101 64-65 0.46951433936486353 1101 66-67 0.46692298472417093 1101 68-69 0.49781293050345 1101 70-71 0.5348414176930554 1101 72-73 0.46981361238343666 1101 74-75 0.46948038949179605 1101 76-77 0.4101075608549003 1101 78-79 0.3908311645103204 1101 80-81 0.3946771761284218 1101 82-83 0.4366021063181371 1101 84-85 0.4381606814895278 1101 86-87 0.4218958805111299 1101 88-89 0.4057818277557921 1101 90-91 0.4456663908779248 1101 92-93 0.45030716434531826 1101 94-95 0.4439871197768177 1101 96-97 0.49289094724557714 1101 98-99 0.4675470705520901 1101 100-101 0.5052980872679598 2202 1 -0.23988437273987273 2202 2 0.003585987768872201 2202 3 1.5966568558064544 2202 4 1.4046598888919704 2202 5 1.3037183221914574 2202 6 1.213672234636043 2202 7 16.646545927852706 2202 8 10.091348515435783 2202 9 3.4254237027504004 2202 10-11 1.2763271198167274 2202 12-13 0.9369747173288516 2202 14-15 1.0973644503919644 2202 16-17 1.0969644932313827 2202 18-19 1.1098647514350262 2202 20-21 1.1075858813432546 2202 22-23 1.0871496936606988 2202 24-25 1.0969166256022618 2202 26-27 1.1105867858829157 2202 28-29 1.1256583226207013 2202 30-31 1.165915523778196 2202 32-33 1.1135849651999692 2202 34-35 1.0858432143698735 2202 36-37 1.089798940376376 2202 38-39 1.1060433321281025 2202 40-41 1.125107592885783 2202 42-43 1.1567224896162145 2202 44-45 1.1505598487445639 2202 46-47 1.114100676029942 2202 48-49 1.1334183578785897 2202 50-51 1.1367553120755929 2202 52-53 1.1391039133162764 2202 54-55 1.1234559984412513 2202 56-57 1.1488868191566155 2202 58-59 1.1657765312136519 2202 60-61 1.1452835048501342 2202 62-63 1.1747522036031626 2202 64-65 1.1817467510326196 2202 66-67 1.193309209830268 2202 68-69 1.2336157979970608 2202 70-71 1.2611003077354503 2202 72-73 1.2540429994174254 2202 74-75 1.1408726814475294 2202 76-77 1.1736508000222123 2202 78-79 1.1821977001128126 2202 80-81 1.2320634122521277 2202 82-83 1.2559255263598637 2202 84-85 1.219558390412228 2202 86-87 1.1998697030485417 2202 88-89 1.164955639853705 2202 90-91 1.1981721612141207 2202 92-93 1.215019278813358 2202 94-95 1.268993591976443 2202 96-97 1.261977230283442 2202 98-99 1.1939218170902883 2202 100-101 1.22599982242307 1102 1 -0.16389065476259645 1102 2 0.015027999160587058 1102 3 1.6312247399950977 1102 4 1.5981411472538838 1102 5 1.363560052589044 1102 6 1.2475213599339057 1102 7 16.373166365178967 1102 8 9.9619252451063 1102 9 3.297085990548915 1102 10-11 1.1960189073230225 1102 12-13 0.9221926879138209 1102 14-15 1.1237665114018753 1102 16-17 1.0560792901012732 1102 18-19 1.1108221058221943 1102 20-21 1.1170111966574083 1102 22-23 1.1199175241679242 1102 24-25 1.1091398739818885 1102 26-27 1.099755219554929 1102 28-29 1.0937150642041544 1102 30-31 1.111391118095142 1102 32-33 1.1195699449963143 1102 34-35 1.1196315040902647 1102 36-37 1.1407937773000612 1102 38-39 1.1432087428649638 1102 40-41 1.144413105714463 1102 42-43 1.1068583941490715 1102 44-45 1.1574309144163735 1102 46-47 1.1492753684365553 1102 48-49 1.1623650309095765 1102 50-51 1.1830042829503782 1102 52-53 1.1280654052452093 1102 54-55 1.1601735659685204 1102 56-57 1.172745348747739 1102 58-59 1.1900744682241182 1102 60-61 1.1973935873151476 1102 62-63 1.2133327531784985 1102 64-65 1.195833456245495 1102 66-67 1.20305167690384 1102 68-69 1.1649885645460145 1102 70-71 1.192914174396737 1102 72-73 1.233473365694696 1102 74-75 1.192280711562784 1102 76-77 1.215579289679706 1102 78-79 1.2082258556954137 1102 80-81 1.211737311061821 1102 82-83 1.186669689437064 1102 84-85 1.1937011035191354 1102 86-87 1.195365075500888 1102 88-89 1.1665989414174724 1102 90-91 1.2285205295282111 1102 92-93 1.2790123934365027 1102 94-95 1.272512393288121 1102 96-97 1.2823370825841849 1102 98-99 1.2723466322484036 1102 100-101 1.233770658485156 2203 1 4.739385677806094 2203 2 2.238608401157567 2203 3 2.627797357709273 2203 4 2.1185631993529697 2203 5 1.8542165315756236 2203 6 1.5951793030582735 2203 7 16.50371369646009 2203 8 10.214913669445231 2203 9 3.7270189167852052 2203 10-11 1.5180144453031446 2203 12-13 1.212314936828264 2203 14-15 1.4337296972744156 2203 16-17 1.347299151710594 2203 18-19 1.392542202160648 2203 20-21 1.4056790830559365 2203 22-23 1.3858849318955109 2203 24-25 1.3853583538632677 2203 26-27 1.428373433022422 2203 28-29 1.3895426515120235 2203 30-31 1.4074041403351742 2203 32-33 1.3872570822248562 2203 34-35 1.4212534891137665 2203 36-37 1.4056631683428265 2203 38-39 1.4163881459664864 2203 40-41 1.422983913521648 2203 42-43 1.3501366829939485 2203 44-45 1.4093501035538978 2203 46-47 1.4196668465325715 2203 48-49 1.4592797194572782 2203 50-51 1.4290922499564545 2203 52-53 1.4427084772067644 2203 54-55 1.4586990085275673 2203 56-57 1.4981794405819286 2203 58-59 1.4342644280723817 2203 60-61 1.4147731408372124 2203 62-63 1.4206242646694136 2203 64-65 1.4145721846943218 2203 66-67 1.443476053774198 2203 68-69 1.4010689161585717 2203 70-71 1.397416670200716 2203 72-73 1.4480546526857445 2203 74-75 1.4353698970432411 2203 76-77 1.4569227945632512 2203 78-79 1.460552108721867 2203 80-81 1.5185328789307952 2203 82-83 1.528326892861994 2203 84-85 1.465083464428421 2203 86-87 1.5248691230195277 2203 88-89 1.5476343656091522 2203 90-91 1.5699078297119335 2203 92-93 1.5589227637957208 2203 94-95 1.5439882025377791 2203 96-97 1.493988106357488 2203 98-99 1.503717570210302 2203 100-101 1.5247319824144157 1103 1 -1.9251495528588194 1103 2 -0.6780945445518611 1103 3 0.707580392137757 1103 4 0.5694482504362881 1103 5 0.5924565809703708 1103 6 0.5703259512495151 1103 7 -17.435493910512967 1103 8 -10.364511947353813 1103 9 -2.39456708683921 1103 10-11 -0.18204992267616404 1103 12-13 0.14296583622468972 1103 14-15 0.11337749706210332 1103 16-17 0.14228130753052426 1103 18-19 0.18376891654320104 1103 20-21 0.16981050973817702 1103 22-23 0.1716921814955299 1103 24-25 0.17267030207200662 1103 26-27 0.1860493102816605 1103 28-29 0.1557122205356265 1103 30-31 0.1981012274585794 1103 32-33 0.19790811722350554 1103 34-35 0.18658359186237305 1103 36-37 0.16660452787296975 1103 38-39 0.1849195401779511 1103 40-41 0.1540331825870993 1103 42-43 0.15696333179129596 1103 44-45 0.20610574120382807 1103 46-47 0.1857936809088514 1103 48-49 0.2528099473890322 1103 50-51 0.21874903921624878 1103 52-53 0.19591754660540062 1103 54-55 0.20712884304190027 1103 56-57 0.20687571922106684 1103 58-59 0.16038711878660905 1103 60-61 0.1822092792291201 1103 62-63 0.1966432615857201 1103 64-65 0.13903158689668516 1103 66-67 0.1343717163601994 1103 68-69 0.13338553233561612 1103 70-71 0.16310007775824786 1103 72-73 0.10591998385319812 1103 74-75 0.096710362584286 1103 76-77 0.058568668832869264 1103 78-79 0.06957112300331048 1103 80-81 0.09255844385570455 1103 82-83 0.1091991592870798 1103 84-85 0.1062829272893957 1103 86-87 0.10499051617633626 1103 88-89 0.07694338058587036 1103 90-91 0.1107926198154523 1103 92-93 0.10682355469360516 1103 94-95 0.14116584084920092 1103 96-97 0.1486822604835467 1103 98-99 0.1561404055043205 1103 100-101 0.17746667097885194 2204 1 0.10958915940653569 2204 2 0.27038828833752504 2204 3 1.6492637268157964 2204 4 1.5239345448455381 2204 5 1.37564901258024 2204 6 1.207670184810027 2204 7 16.61562389842426 2204 8 10.026253457114493 2204 9 3.427015569718165 2204 10-11 1.1992857894319613 2204 12-13 0.8422266836352037 2204 14-15 0.9981104092243029 2204 16-17 0.9480547522675309 2204 18-19 0.947619338383781 2204 20-21 0.9229448158805127 2204 22-23 0.8421607826233881 2204 24-25 0.9271447403067015 2204 26-27 0.9502980879474308 2204 28-29 0.9673760448294999 2204 30-31 0.9763804220559038 2204 32-33 0.9213545852297713 2204 34-35 0.9593926676376014 2204 36-37 0.9168882332991188 2204 38-39 0.9190378136861526 2204 40-41 0.8620865274667722 2204 42-43 0.8900584630410435 2204 44-45 0.9277630275108208 2204 46-47 0.9528761421303713 2204 48-49 0.9745675502622433 2204 50-51 0.9487905438798805 2204 52-53 0.9563490782167818 2204 54-55 0.9752322082135834 2204 56-57 0.9983136333950071 2204 58-59 0.9420902577798458 2204 60-61 0.9359748583567935 2204 62-63 0.9516909109006235 2204 64-65 0.9524653423685834 2204 66-67 0.9706171099091918 2204 68-69 0.982799943428482 2204 70-71 1.0089321678133594 2204 72-73 1.0503104301491994 2204 74-75 0.9721140702910311 2204 76-77 0.999240696636857 2204 78-79 1.0201896732809708 2204 80-81 0.9946743045930582 2204 82-83 1.0513509976980906 2204 84-85 1.0275512081514897 2204 86-87 1.043321807848411 2204 88-89 1.0469439463272217 2204 90-91 1.0720762771082626 2204 92-93 1.058117643953068 2204 94-95 1.0863346692499007 2204 96-97 1.1086619853825113 2204 98-99 1.0759301144360656 2204 100-101 1.0475951608211247 2205 1 -1.7135832789311536 2205 2 -0.6587174249149115 2205 3 0.7570323821816309 2205 4 0.5816576587888562 2205 5 0.4220647412934042 2205 6 0.37261244708589913 2205 7 -17.435493910512967 2205 8 -10.385246698849778 2205 9 -2.5811989196650593 2205 10-11 -0.4033751862280539 2205 12-13 -0.07339671554992577 2205 14-15 -0.15246814570313916 2205 16-17 -0.15018964888449915 2205 18-19 -0.12236081730500814 2205 20-21 -0.07003401392997688 2205 22-23 -0.08680102893310249 2205 24-25 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-1.3226633787638207 1213 7 15.524568879995066 1213 8 8.251957384150309 1213 9 1.6603375326172412 1213 10-11 -0.60001000681401 1213 12-13 -1.1040003210097353 1213 14-15 -1.341888100697048 1213 16-17 -1.3939641302796986 1213 18-19 -1.424819356725358 1213 20-21 -1.4419498178519206 1213 22-23 -1.4590689872266793 1213 24-25 -1.4869197501348594 1213 26-27 -1.5043596433695683 1213 28-29 -1.496779908860212 1213 30-31 -1.5104837873032224 1213 32-33 -1.5079776999369514 1213 34-35 -1.6458482036724291 1213 36-37 -1.6363622077482347 1213 38-39 -1.634144873985079 1213 40-41 -1.6346423861384025 1213 42-43 -1.651786231459866 1213 44-45 -1.6014310728976398 1213 46-47 -1.5792070197212098 1213 48-49 -1.5546583353917711 1213 50-51 -1.6293304806201405 1213 52-53 -1.7167803242889335 1213 54-55 -1.6324114610845726 1213 56-57 -1.5928745319609376 1213 58-59 -1.596507522855724 1213 60-61 -1.6616098504612218 1213 62-63 -1.6350074930663467 1213 64-65 -1.6335496893982224 1213 66-67 -1.7021256854713087 1213 68-69 -1.6785200920713805 1213 70-71 -1.6316833165787372 1213 72-73 -1.692690162943574 1213 74-75 -1.7173525871848234 1213 76-77 -1.6902768231306453 1213 78-79 -1.6829381328187587 1213 80-81 -1.7437051356535065 1213 82-83 -1.793993882843587 1213 84-85 -1.7673187059733237 1213 86-87 -1.7979948161471597 1213 88-89 -1.8267698896247602 1213 90-91 -1.8757256771944313 1213 92-93 -1.974833881749511 1213 94-95 -2.050617137530839 1213 96-97 -2.1276742034290947 1213 98-99 -2.179926197506216 1213 100-101 -2.2124381496605636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66003.0 3 186916.0 4 36271.0 5 15291.0 6 9698.0 7 7517.0 8 9822.0 9 35921.0 10 246399.0 11 925227.0 12 1769846.0 13 2093823.0 14 1872247.0 15 1538363.0 16 1308819.0 17 1178559.0 18 1106855.0 19 1075558.0 20 1077782.0 21 1096040.0 22 1131415.0 23 1198680.0 24 1271773.0 25 1351118.0 26 1493713.0 27 1753685.0 28 2257369.0 29 3092410.0 30 3669755.0 31 3978310.0 32 3380468.0 33 4452894.0 34 6698275.0 35 1.1202917E7 36 2.1005738E7 37 3.395534E7 38 1.0720554E7 39 9112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.325890742467994 19.59791829775112 27.3185089737394 27.757681986041483 2 22.154462008037914 6.1641196064576205 24.980549998467954 46.700868387036515 3 13.609747111674379 32.47060398195995 25.6400560827483 28.27959282361737 4 19.650486362966475 27.081457175184003 22.30455178162181 30.96350468022771 5 38.44616314047642 25.03775574802868 16.962827596559073 19.553253514935832 6 25.13818822449983 37.57306309806313 28.482213720745147 8.806534956691891 7 0.47430199037347515 0.4485661872927328 98.4481946753719 0.6289371469619056 8 41.18001662686579 12.89627114900207 9.558384816163626 36.365327407968515 9 25.949915958082276 10.559822812173467 27.681202036126614 35.80905919361765 10-11 27.75756203929233 13.768608044864264 17.135228233174043 41.338601682669356 12-13 27.202502574053273 23.39544786370407 24.110662410545928 25.291387151696725 14-15 21.21473748389221 23.70710792199981 34.49277230307802 20.585382291029962 16-17 20.180078551435713 27.512136784239015 31.559660031187704 20.748124633137575 18-19 23.37552610077892 27.905884319875806 26.688870877851567 22.029718701493703 20-21 21.436739391692132 25.684513228232447 29.046611634046144 23.83213574602928 22-23 20.242714447162687 29.962326179893683 28.03419613811005 21.760763234833583 24-25 21.128885671489694 28.741414551816508 29.422026808765672 20.707672967928122 26-27 20.819672968580395 28.413875270328237 29.99840977011104 20.76804199098033 28-29 21.70781366893486 30.151683018367926 27.764163706089818 20.3763396066074 30-31 21.48572868185424 28.088840104278162 28.943338811491493 21.482092402376107 32-33 21.64164507268169 28.07236560826112 29.24534139479414 21.04064792426305 34-35 21.55838348884234 28.123300092858756 29.335010513814524 20.983305904484382 36-37 22.6851731555974 25.715402313945113 30.115933139152624 21.483491391304867 38-39 20.929202612693707 28.788511616868647 26.923100990019588 23.35918478041806 40-41 20.391545283539948 29.800904108059157 27.36123918920153 22.446311419199365 42-43 21.6279159909789 27.15418566867821 26.887526371996035 24.330371968346853 44-45 21.72108285999963 28.249277407799923 27.04998187620144 22.97965785599901 46-47 20.14901025634914 29.616737801177432 28.13712178826473 22.097130154208696 48-49 20.61893819958366 27.438220812110064 27.214469192937468 24.72837179536881 50-51 20.54592770418501 28.567938143652615 26.69768773977328 24.18844641238909 52-53 19.87963401719261 28.22754469116952 28.667875345906456 23.224945945731406 54-55 20.883465929313797 25.798051802956866 29.3302515142509 23.98823075347844 56-57 21.355366199515423 25.619020887801515 30.48306235584895 22.542550556834122 58-59 22.16534054445317 26.734189422466258 26.694999091844153 24.40547094123642 60-61 23.927833267255995 25.357450782912828 28.858082860081907 21.85663308974927 62-63 25.567590280188867 24.67189547876098 27.66098405879517 22.099530182254988 64-65 21.72701751431523 26.09860244651829 28.200959015087335 23.973421024079148 66-67 21.810780757903157 29.172631127595857 26.96064885561478 22.055939258886205 68-69 22.93112332893594 28.77386345124962 25.857790718926683 22.43722250088776 70-71 21.581222846353672 28.094089970737812 25.910641932238153 24.41404525067036 72-73 24.036756012673557 24.4436354303072 27.062129495047994 24.457479061971245 74-75 20.841932065777716 26.478093061214025 25.055912598749718 27.624062274258538 76-77 23.366623101686525 27.086451063172806 24.92497074631941 24.62195508882126 78-79 23.277224830316985 28.694695232106888 25.75476021035467 22.273319727221462 80-81 25.61203450769919 26.207607136214122 25.60452921104801 22.575829145038682 82-83 25.854878264644732 27.044736630772586 26.57767119449544 20.522713910087244 84-85 23.663763196601927 23.88049386045704 29.56480794349423 22.890934999446795 86-87 21.969866414431596 27.29726989638323 26.56780343159871 24.165060257586465 88-89 21.96511606899126 28.047312785260885 26.263806297505266 23.72376484824259 90-91 22.12493407051975 26.153564685098008 28.24208384004214 23.4794174043401 92-93 23.206661866975615 27.02447197700056 26.299564022636048 23.469302133387778 94-95 21.839711582987718 29.467585774169464 25.823329558713336 22.869373084129478 96-97 23.241014152191127 29.30477828978878 25.822530321407267 21.631677236612823 98-99 23.025948095027513 27.905471445229814 25.870554119212002 23.198026340530674 100-101 22.566402373270193 27.91754024462596 28.228644134112823 21.28741324799102 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 242.0 1 4163.5 2 35920.5 3 145225.5 4 159815.0 5 70123.0 6 36692.0 7 22729.0 8 17314.0 9 14088.0 10 12258.5 11 11066.0 12 10569.0 13 10566.0 14 10641.5 15 11408.5 16 13361.5 17 19221.5 18 47064.0 19 107876.5 20 108501.5 21 79642.0 22 90099.5 23 105573.0 24 137395.0 25 166486.5 26 198741.5 27 263268.5 28 337872.5 29 434753.0 30 620540.0 31 764025.0 32 867069.0 33 1023435.5 34 1238651.0 35 1570796.5 36 1891883.5 37 2221180.5 38 2732100.0 39 3406078.0 40 4110667.0 41 4501821.0 42 5041442.0 43 6195888.5 44 7235248.0 45 8545754.5 46 9459879.0 47 1.010691E7 48 9259057.5 49 6863939.5 50 5616313.0 51 5017715.5 52 4477298.5 53 4026205.5 54 3494652.0 55 2804694.0 56 2340690.5 57 2280880.0 58 1989397.0 59 1404074.5 60 1016155.0 61 767165.5 62 616084.5 63 452220.5 64 346948.0 65 273297.5 66 222039.5 67 180949.5 68 145188.0 69 115383.5 70 89852.5 71 68231.0 72 51647.5 73 39067.0 74 28821.5 75 20973.0 76 14967.5 77 10112.0 78 6868.0 79 4758.5 80 3288.5 81 2321.0 82 1601.5 83 1140.5 84 836.0 85 629.0 86 501.5 87 399.5 88 346.5 89 324.5 90 344.0 91 359.0 92 357.0 93 363.5 94 374.5 95 428.5 96 535.0 97 587.0 98 646.0 99 1326.0 100 1584.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.07211229474401028 2 0.016255785379292655 3 0.011148227435345718 4 0.03354056594875777 5 0.05575906663837553 6 0.07236018903982455 7 50.11512156529688 8 0.16795773991589977 9 0.19560263115005577 10-11 0.2359181949759263 12-13 0.23381148323241033 14-15 0.24331526721800695 16-17 0.2650305736687942 18-19 0.28508506629180685 20-21 0.27788794652418014 22-23 0.26332142824875393 24-25 0.2650566883194539 26-27 0.2747405464633307 28-29 0.28965435061544004 30-31 0.2816270188193788 32-33 0.2809534946949023 34-35 0.2733860925671756 36-37 0.26186251574996017 38-39 0.2744412024080078 40-41 0.2763580957206093 42-43 0.27767084412989 44-45 0.27547643393266613 46-47 0.28395161249899564 48-49 0.2826041744791372 50-51 0.26724914966215085 52-53 0.2732173217651511 54-55 0.27147465604724924 56-57 0.2569611466149531 58-59 0.26066474975776327 60-61 0.2558046963387252 62-63 0.24274269375802088 64-65 0.25387026333538204 66-67 0.25199780390599247 68-69 0.2676650352181789 70-71 0.27179076025150295 72-73 0.2769213146788666 74-75 0.2792985274307082 76-77 0.27950120830149977 78-79 0.27378287934884066 80-81 0.2734862636898553 82-83 0.27418629223589686 84-85 0.2471529515522638 86-87 0.246120448423943 88-89 0.2637030139651096 90-91 0.2663690469578291 92-93 0.25012222630935993 94-95 0.256102871081332 96-97 0.24346104153661471 98-99 0.23208206972529094 100-101 0.24157026287467284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.28280483E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.53975404036156 #Duplication Level Percentage of deduplicated Percentage of total 1 73.55920366148892 19.5224317258078 2 9.892170461947122 5.250715419708129 3 4.601519028782667 3.6636954970780615 4 2.270861620176023 2.4107243543667445 5 1.448005976725037 1.9214861235628 6 1.1542480340644006 1.8380075355384038 7 0.8544732359476978 1.5874256661286612 8 0.7982858120002684 1.6949047283517946 9 0.6046695568974475 1.4443003184177403 >10 4.19078060537518 20.409275963102324 >50 0.35060171096936443 6.447906356553303 >100 0.22743979162359917 12.059963514348086 >500 0.026805756324237134 4.905816405564621 >1k 0.01882187225989561 9.481137468470378 >5k 0.0014302941127893288 2.5848390143349196 >10k+ 6.825813051539338E-4 4.777369908666222 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCAGANCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 402529 0.313788185534038 No Hit ACTTGANCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 265643 0.2070798252295324 No Hit ACAGTGNCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 244050 0.19024717891029455 No Hit CGATGTNCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 208009 0.16215171250953272 No Hit CTCAGANGCCCACAGTAACGGACAGCTTTACCTGTTTCTATGGAGACAAC 167883 0.13087181781191143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0134043539577256E-5 0.0 0.0 2 1.0134043539577256E-5 0.0 0.0 3 1.0134043539577256E-5 0.0 0.0 4 2.284057505458566E-4 0.0 0.0 5 2.3230345959954018E-4 0.0 0.0 6 2.3230345959954018E-4 2.338625432210136E-6 7.795418107367119E-7 7 2.9076909540479357E-4 2.338625432210136E-6 7.795418107367119E-7 8 0.0017555281577790753 7.79541810736712E-6 7.795418107367119E-7 9 0.0019317046070055723 7.79541810736712E-6 7.795418107367119E-7 10-11 0.002188563633643319 8.185189012735477E-6 1.169312716105068E-6 12-13 0.0154201165581829 9.7442726342089E-6 1.94885452684178E-6 14-15 0.18410438944168928 1.2472668971787391E-5 2.338625432210136E-6 16-17 0.18420534010617967 1.2472668971787391E-5 2.728396337578492E-6 18-19 0.1842988851234681 1.3252210782524104E-5 7.015876296630408E-6 20-21 0.1843799574717847 1.3252210782524104E-5 9.7442726342089E-6 22-23 0.18444465944207583 1.4031752593260816E-5 1.169312716105068E-5 24-25 0.1845155977468529 2.1827170700627935E-5 1.9098774363049444E-5 26-27 0.18461732795315403 2.1827170700627935E-5 2.1827170700627935E-5 28-29 0.18468865602883644 2.1827170700627935E-5 2.1827170700627935E-5 30-31 0.18475218868641147 2.1827170700627935E-5 2.338625432210136E-5 32-33 0.1848367689728764 2.1827170700627935E-5 2.416579613283807E-5 34-35 0.18530332474660233 2.1827170700627935E-5 2.4555567038206426E-5 36-37 0.18626605888286218 2.1827170700627935E-5 2.611465065967985E-5 38-39 0.18838368421172846 2.2606712511364647E-5 2.8843046997258344E-5 40-41 0.19197542310469784 2.416579613283807E-5 2.8843046997258344E-5 42-43 0.1991140772365193 2.416579613283807E-5 2.9622588807995056E-5 44-45 0.21085787461526784 2.416579613283807E-5 3.0402130618731768E-5 46-47 0.22537060450575322 2.416579613283807E-5 3.0402130618731768E-5 48-49 0.24888431391391003 2.5335108848943138E-5 3.0402130618731768E-5 50-51 0.2847097169099371 2.650442156504821E-5 3.079190152410013E-5 52-53 0.3309938426097133 2.650442156504821E-5 3.118167242946848E-5 54-55 0.3906330786110308 2.650442156504821E-5 3.118167242946848E-5 56-57 0.4554827720753125 2.728396337578492E-5 3.6638465104625465E-5 58-59 0.532118747947028 2.8063505186521632E-5 3.6638465104625465E-5 60-61 0.621780087934343 2.8843046997258344E-5 3.6638465104625465E-5 62-63 0.7344733804907797 2.8843046997258344E-5 4.0536174158309023E-5 64-65 0.8742951178317593 2.9622588807995056E-5 4.092594506367738E-5 66-67 1.0389612424518233 2.9622588807995056E-5 4.1315715969045735E-5 68-69 1.2176735411886468 2.9622588807995056E-5 4.1315715969045735E-5 70-71 1.4174630914041695 2.9622588807995056E-5 4.443388321199258E-5 72-73 1.6595462927903069 2.9622588807995056E-5 4.755205045493943E-5 74-75 1.937674338192194 2.9622588807995056E-5 4.755205045493943E-5 76-77 2.2562531979241145 2.9622588807995056E-5 4.755205045493943E-5 78-79 2.603152032098289 2.9622588807995056E-5 4.833159226567614E-5 80-81 2.9575173177356993 2.9622588807995056E-5 4.911113407641285E-5 82-83 3.3149918058852332 2.9622588807995056E-5 4.911113407641285E-5 84-85 3.7157351520106143 2.9622588807995056E-5 4.9890675887149564E-5 86-87 4.137062689419404 2.9622588807995056E-5 4.9890675887149564E-5 88-89 4.5940842770291095 3.0012359713363412E-5 4.9890675887149564E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCAGAT 986540 0.0 176.40622 1 ACAGTGT 689890 0.0 167.15411 1 CGATGTT 724745 0.0 166.23663 1 GATCAGT 225340 0.0 163.46883 1 GACCATG 338865 0.0 162.34987 2 TCAGATG 369320 0.0 161.74602 2 CTCAGTC 229725 0.0 161.70102 2 CTCAGTG 297440 0.0 161.23071 2 GCTCAGT 359160 0.0 157.61417 1 TCTCAGT 308940 0.0 155.84581 1 GTGGCCT 358755 0.0 155.70772 1 CAGTGTG 327660 0.0 155.29083 2 ACTTGAT 650755 0.0 153.48334 1 GCCAATT 487030 0.0 151.52187 1 TCAGTCG 82195 0.0 150.61035 3 AGTGTGC 150935 0.0 150.0858 3 GATCTCC 131165 0.0 147.97237 5 TGGCCTG 255010 0.0 147.67719 2 TGGCCTC 212840 0.0 146.66753 2 TGACCAT 754435 0.0 145.57669 1 >>END_MODULE