FastQCFastQC Report
Mon 29 Sep 2014
Complete_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128280483
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGANCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA4025290.313788185534038No Hit
ACTTGANCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA2656430.2070798252295324No Hit
ACAGTGNCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA2440500.19024717891029455No Hit
CGATGTNCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA2080090.16215171250953272No Hit
CTCAGANGCCCACAGTAACGGACAGCTTTACCTGTTTCTATGGAGACAAC1678830.13087181781191143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT9865400.0176.406221
ACAGTGT6898900.0167.154111
CGATGTT7247450.0166.236631
GATCAGT2253400.0163.468831
GACCATG3388650.0162.349872
TCAGATG3693200.0161.746022
CTCAGTC2297250.0161.701022
CTCAGTG2974400.0161.230712
GCTCAGT3591600.0157.614171
TCTCAGT3089400.0155.845811
GTGGCCT3587550.0155.707721
CAGTGTG3276600.0155.290832
ACTTGAT6507550.0153.483341
GCCAATT4870300.0151.521871
TCAGTCG821950.0150.610353
AGTGTGC1509350.0150.08583
GATCTCC1311650.0147.972375
TGGCCTG2550100.0147.677192
TGGCCTC2128400.0146.667532
TGACCAT7544350.0145.576691
Produced by FastQC (version 0.11.2)