##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Complete_Undetermined_L001_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 122850999 Sequences flagged as poor quality 0 Sequence length 7 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.648033338336955 33.0 33.0 33.0 22.0 33.0 2 30.642080330172977 33.0 33.0 33.0 22.0 33.0 3 30.66209540550826 33.0 33.0 33.0 22.0 33.0 4 34.17997136514942 37.0 37.0 37.0 27.0 37.0 5 34.19657521873306 37.0 37.0 37.0 27.0 37.0 6 34.12090667655051 37.0 37.0 37.0 27.0 37.0 7 28.312144836526727 33.0 27.0 33.0 15.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.6804813538962762 2201 2 0.6996863164310447 2201 3 0.6652714670390587 2201 4 0.8075589644914913 2201 5 0.8112308346578345 2201 6 0.8057126868085405 2201 7 1.1327148826889726 1101 1 0.04044221070835263 1101 2 0.06960272724325378 1101 3 0.04266433719279661 1101 4 0.059809562092375756 1101 5 0.04510626993121747 1101 6 0.025618840951899813 1101 7 1.443868137316798 2202 1 0.3072393387491772 2202 2 0.31829927859343243 2202 3 0.30634736038204124 2202 4 0.36605241668861765 2202 5 0.3517134719128805 2202 6 0.34903931048982173 2202 7 1.575159326977147 1102 1 0.3881039044087409 1102 2 0.41167792851390317 1102 3 0.4075566221707234 1102 4 0.4568966216227821 1102 5 0.4494600958769155 1102 6 0.4281838512771614 1102 7 1.5683160993328755 2203 1 0.37210222337510146 2203 2 0.3922439844410164 2203 3 0.39272795739211475 2203 4 0.4428844544111996 2203 5 0.4351734914446439 2203 6 0.4617028823419318 2203 7 1.5441067325606035 1103 1 0.576286862351747 1103 2 0.5908475033870992 1103 3 0.5761296625430781 1103 4 0.651690546943307 1103 5 0.6516740988546701 1103 6 0.6682367138602174 1103 7 1.447188920979464 2204 1 0.23333378880511546 2204 2 0.2919958900005746 2204 3 0.2854592132103164 2204 4 0.29930485716602107 2204 5 0.32966660461480046 2204 6 0.28573153211513613 2204 7 1.1072122468511978 2205 1 0.2881852216877121 2205 2 0.333199846065348 2205 3 0.3208087124605896 2205 4 0.3787987163873652 2205 5 0.311565414966708 2205 6 0.3408005833486456 2205 7 0.9645013899847434 1216 1 0.016635615707649265 1216 2 0.028949969165733336 1216 3 0.006619428693870333 1216 4 0.03218254187707714 1216 5 0.00909201571083429 1216 6 0.01834501509223685 1216 7 0.525409332487186 2206 1 0.4444479850987051 2206 2 0.4397606025914058 2206 3 0.43430400902135347 2206 4 0.5020478000003337 2206 5 0.49596664964914794 2206 6 0.5083602032856689 2206 7 0.7544777378635068 2207 1 0.3096904697327467 2207 2 0.2601904279687979 2207 3 0.2759616243609315 2207 4 0.32026584368207267 2207 5 0.3426879224864763 2207 6 0.35754519288551734 2207 7 0.3880269427637941 1116 1 -0.9767200786948251 1116 2 -1.006282424025084 1116 3 -0.9920407329525212 1116 4 -1.1953868965424874 1116 5 -1.1994014413333574 1116 6 -1.2340555977251526 1116 7 -1.1415432525545945 1113 1 -0.5198418075320888 1113 2 -0.5262388289638196 1113 3 -0.5632434558663135 1113 4 -0.6350631394049202 1113 5 -0.6426787942213537 1113 6 -0.626330413838275 1113 7 -0.930250442616245 1112 1 -0.3004257967004911 1112 2 -0.2983699548464749 1112 3 -0.3041868517136166 1112 4 -0.37496119195338196 1112 5 -0.3445756390775827 1112 6 -0.3344280787343834 1112 7 -1.1788285285046172 1115 1 -0.5264600941684456 1115 2 -0.5442010952958789 1115 3 -0.5736306825252662 1115 4 -0.6691272218311397 1115 5 -0.7162036813109225 1115 6 -0.6841653193327559 1115 7 -1.774356821784881 1114 1 -0.3077911203729933 1114 2 -0.26921795301550944 1114 3 -0.34154467833892355 1114 4 -0.4418855334010132 1114 5 -0.425883103567287 1114 6 -0.4249549361675662 1114 7 -1.79511263765108 1109 1 0.505609527440221 1109 2 0.5332109233065303 1109 3 0.510095838233795 1109 4 0.6297510605350638 1109 5 0.6228804450130738 1109 6 0.6582482014468809 1109 7 1.7237299359791898 1108 1 0.5587127174788371 1108 2 0.5655419395012622 1108 3 0.55507791046373 1108 4 0.6625071115014691 1108 5 0.6701363341706852 1108 6 0.6760721115538715 1108 7 1.4967916999754394 1111 1 0.4957685067065718 1111 2 0.5169510544694873 1111 3 0.503895807750343 1111 4 0.6287185177590615 1111 5 0.6011803153840773 1111 6 0.6138232908720411 1111 7 1.6139587415103662 1110 1 0.3090541601690866 1110 2 0.3209136751174171 1110 3 0.32202079516223847 1110 4 0.38881148353056005 1110 5 0.39307537450672925 1110 6 0.39719939196263 1110 7 1.353478111575555 1105 1 0.3003573669616735 1105 2 0.31571403610839965 1105 3 0.27468716097542867 1105 4 0.35022816904413645 1105 5 0.3575973135891104 1105 6 0.3581953202060646 1105 7 1.355482835686601 1104 1 -0.2400901414451262 1104 2 -0.22432423230261733 1104 3 -0.2179294385546875 1104 4 -0.26310796198761466 1104 5 -0.22661283788311692 1104 6 -0.2623190803985054 1104 7 0.6100114589018908 1107 1 0.20805060344335757 1107 2 0.22561895165207702 1107 3 0.2095845642901537 1107 4 0.25285671689160694 1107 5 0.22520117204766166 1107 6 0.23053842737654406 1107 7 0.6602314293491887 1106 1 0.13915097885429617 1106 2 0.16101834430886086 1106 3 0.1357294855413329 1106 4 0.1513744032609523 1106 5 0.1733401189336874 1106 6 0.1772737947318248 1106 7 1.1157883491966452 2103 1 0.29365842422195243 2103 2 0.325709120556791 2103 3 0.3029127056252996 2103 4 0.3989716827210046 2103 5 0.33052670361333725 2103 6 0.38830558086264233 2103 7 1.0824568704660535 2102 1 0.23204520516753746 2102 2 0.26455163755425914 2102 3 0.20695343722146475 2102 4 0.27094739758252473 2102 5 0.2510797179594988 2102 6 0.267584544342661 2102 7 0.9117430885395414 2101 1 -0.2595433091332211 2101 2 -0.22309157901161925 2101 3 -0.2546580042587223 2101 4 -0.2692620261022185 2101 5 -0.30514841549666727 2101 6 -0.3165100902013265 2101 7 0.5266734881797959 2107 1 -1.1669453020926888 2107 2 -1.1024275563688875 2107 3 -1.1073198780286724 2107 4 -1.3727631304762227 2107 5 -1.348201481743729 2107 6 -1.4228379356820469 2107 7 -0.675409387530781 2106 1 -0.5755205428460286 2106 2 -0.5492474733097268 2106 3 -0.5685240572359689 2106 4 -0.6549031695316998 2106 5 -0.7036694701978305 2106 6 -0.7199934421542764 2106 7 -0.04145921507077688 2105 1 -0.23832874403731452 2105 2 -0.22594836711318678 2105 3 -0.26575038408567053 2105 4 -0.29380039890303067 2105 5 -0.31404654618344097 2105 6 -0.31378959317515154 2105 7 -0.02985783249221896 2104 1 -0.97188866272338 2104 2 -0.9231219635850145 2104 3 -0.9542508812485373 2104 4 -1.1356313134892915 2104 5 -1.1589800289065764 2104 6 -1.198293343873246 2104 7 -0.9889741241771794 2111 1 -0.5611937471130695 2111 2 -0.534318189708916 2111 3 -0.5750154909629721 2111 4 -0.7134368966881937 2111 5 -0.7138925015005597 2111 6 -0.6755574821042174 2111 7 -0.8465242931868247 2110 1 0.5743072333060155 2110 2 0.20787196536545238 2110 3 0.4822946689553973 2110 4 0.7256500169956013 2110 5 0.7405429012576121 2110 6 0.7569244238465274 2110 7 0.05713266093752267 2109 1 0.4336491078973843 2109 2 0.4423596257325926 2109 3 0.4399136334203604 2109 4 0.49579436392426146 2109 5 0.46871458477536976 2109 6 0.4667076552217253 2109 7 -0.8892560216846377 2108 1 0.4102367082627367 2108 2 0.4240016954081902 2108 3 0.3947301885279373 2108 4 0.5024704034115004 2108 5 0.46457641829233864 2108 6 0.4745723337706025 2108 7 0.5262672829542119 2216 1 0.3600526314418886 2216 2 0.3828371488077096 2216 3 0.36906228938055463 2216 4 0.43982269860738654 2216 5 0.426243586542725 2216 6 0.42533804641172424 2216 7 0.5376990431997903 2211 1 0.41531565155704087 2211 2 0.4417440428130135 2211 3 0.4050659667817911 2211 4 0.5095855753979066 2211 5 0.5135748760857481 2211 6 0.5153342958306268 2211 7 0.34984017800059775 2210 1 -0.0021045262817871446 2210 2 -0.00936592716591278 2210 3 0.033284303405267224 2210 4 0.06851367643058381 2210 5 0.02676191408995976 2210 6 0.04300888261127511 2210 7 0.09122160156840309 2209 1 0.2583785748790426 2209 2 0.29299200370954637 2209 3 0.2835663425020343 2209 4 0.34548698300687164 2209 5 0.31659502544764706 2209 6 0.3143587549364071 2209 7 0.3026392658294341 2208 1 0.3239594973563307 2208 2 0.33523427790759897 2208 3 0.2975725517799539 2208 4 0.3522052744906716 2208 5 0.3509570282524521 2208 6 0.35838165513869313 2208 7 0.4616148774802795 2215 1 0.1436123945910026 2215 2 0.16178626464147072 2215 3 0.11254989336360666 2215 4 0.22403864601837142 2215 5 0.22898509081042562 2215 6 0.24413373807556837 2215 7 -0.48517807504075705 2214 1 0.3978109754147958 2214 2 0.39475579831674423 2214 3 0.38726513153867614 2214 4 0.48633781525134623 2214 5 0.4628551385306139 2214 6 0.4691294375073767 2214 7 0.2865266546882417 2213 1 0.16000240626355833 2213 2 0.1698939248687914 2213 3 0.14309488437858775 2213 4 0.16207399353236696 2213 5 0.1781231823927456 2213 6 0.18914258117899863 2213 7 -0.15970864593270306 2212 1 -0.3031059326469858 2212 2 -0.31413166370294476 2212 3 -0.29163830403063074 2212 4 -0.36891076699679104 2212 5 -0.3850863278092689 2212 6 -0.4830443810329186 2212 7 -0.6202778333900589 2116 1 -0.22232783617467788 2116 2 -0.21328946681678929 2116 3 -0.2104517251044271 2116 4 -0.24841197369119072 2116 5 -0.25906719775765197 2116 6 -0.30184148278273426 2116 7 -0.5800401503223114 2112 1 -0.41771881565731306 2112 2 -0.4347304967675498 2112 3 -0.39776854223416436 2112 4 -0.5099620205352906 2112 5 -0.4913495699092252 2112 6 -0.4886064192158628 2112 7 -1.6674256274943673 2113 1 -0.5231258808007304 2113 2 -0.4887119179878283 2113 3 -0.5448278625880931 2113 4 -0.6242271317147754 2113 5 -0.6264851414151096 2113 6 -0.6637750472440374 2113 7 -1.5921652279718828 2114 1 -0.793487538394519 2114 2 -0.8075078893299654 2114 3 -0.7667903354890377 2114 4 -0.9437719402062541 2114 5 -0.9138193844655689 2114 6 -0.9065122430250199 2114 7 -2.8509502653619805 2115 1 0.4441816439073243 2115 2 -0.4092129077441804 2115 3 0.22515458459754 2115 4 0.5792493867409405 2115 5 0.6345293937973295 2115 6 0.6772562005245248 2115 7 -0.10068249349485114 1202 1 0.3333863305308604 1202 2 0.350563545360572 1202 3 0.3384158446890524 1202 4 0.4534567414851409 1202 5 0.4502779017381968 1202 6 0.41043267144548423 1202 7 0.05622864074700118 1203 1 0.3527176553549687 1203 2 0.3874454706913717 1203 3 0.3652318623358539 1203 4 0.429562154604497 1203 5 0.45097357912477065 1203 6 0.447411468366262 1203 7 0.6833018988431725 1201 1 0.28877176766071955 1201 2 0.34578099121894823 1201 3 0.34455409226104905 1201 4 0.3494960195707577 1201 5 0.40840170671783227 1201 6 0.41069915458192696 1201 7 0.1312984715681651 1206 1 0.5500053723514675 1206 2 0.574640252637483 1206 3 0.53532748803066 1206 4 0.647701082678509 1206 5 0.6601406469896176 1206 6 0.6579273724928143 1206 7 0.6200551663872851 1207 1 0.34142177986029054 1207 2 0.3372878336935763 1207 3 0.3162162516759359 1207 4 0.4050007850604871 1207 5 0.3711409432379327 1207 6 0.392453723443829 1207 7 0.357584901062431 1204 1 0.2736595329053486 1204 2 0.30337983439559224 1204 3 0.28009667126781324 1204 4 0.33084800384850155 1204 5 0.33541474308494656 1204 6 0.33141486523917507 1204 7 -0.013517484228771082 1205 1 0.04480208724221413 1205 2 0.05893557741742583 1205 3 0.06655338532278421 1205 4 0.09578630454352322 1205 5 0.07629698815722463 1205 6 0.05781298763071874 1205 7 0.004338539145411602 1210 1 0.3521633688953685 1210 2 0.3708412606248821 1210 3 0.3660295798362654 1210 4 0.44544940570601455 1210 5 0.429186312639537 1210 6 0.4360838678948866 1210 7 0.07250606455399833 1211 1 -0.23514878373973502 1211 2 -0.20720061671491408 1211 3 -0.19944462108880856 1211 4 -0.2203907925769073 1211 5 -0.2421306057092636 1211 6 -0.27733675017460513 1211 7 -0.7102625112900682 1208 1 -0.21222386208610544 1208 2 -0.1386730699054759 1208 3 -0.11166454288813554 1208 4 -0.7296313044264124 1208 5 -0.35136887180840404 1208 6 -0.1921352023523326 1208 7 -0.6657715540699485 1209 1 -0.2776962250970918 1209 2 -0.23694906715284247 1209 3 -0.22974104566957365 1209 4 -0.2595840286426636 1209 5 -0.27763123519711996 1209 6 -0.28414391563075014 1209 7 -1.0087817877211762 1214 1 -0.751435041781523 1214 2 -0.7185468132442487 1214 3 -0.7279920512267104 1214 4 -0.8935464288657542 1214 5 -0.8864270450669665 1214 6 -0.8890835352179707 1214 7 -1.5568395579510899 1215 1 -0.17023483105762693 1215 2 -0.16455980999423758 1215 3 -0.17943381445858364 1215 4 -0.20540076745728442 1215 5 -0.22955033364648614 1215 6 -0.21499284479646974 1215 7 -1.397182579032954 1212 1 -1.4503174807948973 1212 2 -1.3957462737834625 1212 3 -1.4055380500895929 1212 4 -1.7367183367591608 1212 5 -1.7445515715445126 1212 6 -1.7421279034173338 1212 7 -2.740608674314551 1213 1 -1.1541150832697369 1213 2 -1.0826201327308524 1213 3 -1.137402283141821 1213 4 -1.3403038273109686 1213 5 -1.3458851015369078 1213 6 -1.4382065536841964 1213 7 -2.9986179812606437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 165446.0 3 20786.0 4 1025.0 5 8552.0 6 43974.0 7 253327.0 8 477986.0 9 534690.0 10 354835.0 11 441238.0 12 626921.0 13 651573.0 14 320047.0 15 1874134.0 16 1316639.0 17 571003.0 18 1005624.0 19 818394.0 20 725235.0 21 1293837.0 22 879718.0 23 745735.0 24 742721.0 25 909819.0 26 914431.0 27 1229782.0 28 1127286.0 29 1379974.0 30 2178778.0 31 2991052.0 32 9283703.0 33 3.0655541E7 34 5.2413379E7 35 5893814.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.58802436663072 25.402335744740046 28.52011234085785 20.48952754777138 2 27.747008542827068 25.739874707377393 26.923804375212512 19.58931237458303 3 27.927737692786863 25.973044376830263 27.015543183799934 19.08367474658294 4 27.5271871502045 26.522914378521445 26.444063234689025 19.505835236585025 5 27.324549582224396 27.220009678385683 25.623312480902378 19.832128258487547 6 27.726333011463755 27.934477804228212 25.513292997321464 18.825896186986572 7 1.1076432035497832 96.65694674934119 0.9663651900862583 1.269044857022773 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3617842.0 1 3617842.0 2 3617842.0 3 3617842.0 4 3617842.0 5 3617842.0 6 3617842.0 7 1.01977215E7 8 1.6777601E7 9 1.6777601E7 10 1.6777601E7 11 1.6777601E7 12 1.6777601E7 13 1.6777601E7 14 1.6777601E7 15 1.6777601E7 16 1.6777601E7 17 1.6777601E7 18 1.6777601E7 19 1.6777601E7 20 1.6777601E7 21 2.43276715E7 22 3.1877742E7 23 3.1877742E7 24 3.1877742E7 25 3.1877742E7 26 3.1877742E7 27 3.1877742E7 28 3.1877742E7 29 3.1877742E7 30 3.1877742E7 31 3.1877742E7 32 3.1877742E7 33 3.1877742E7 34 3.1877742E7 35 3.1877742E7 36 3.2520026E7 37 3.316231E7 38 3.316231E7 39 3.316231E7 40 3.316231E7 41 3.316231E7 42 3.316231E7 43 3.316231E7 44 3.316231E7 45 3.316231E7 46 3.316231E7 47 3.316231E7 48 3.316231E7 49 3.316231E7 50 2.8036184E7 51 2.2910058E7 52 2.2910058E7 53 2.2910058E7 54 2.2910058E7 55 2.2910058E7 56 2.2910058E7 57 2.2910058E7 58 2.2910058E7 59 2.2910058E7 60 2.2910058E7 61 2.2910058E7 62 2.2910058E7 63 2.2910058E7 64 1.69970885E7 65 1.1084119E7 66 1.1084119E7 67 1.1084119E7 68 1.1084119E7 69 1.1084119E7 70 1.1084119E7 71 1.1084119E7 72 1.1084119E7 73 1.1084119E7 74 1.1084119E7 75 1.1084119E7 76 1.1084119E7 77 1.1084119E7 78 1.1084119E7 79 7187576.5 80 3291034.0 81 3291034.0 82 3291034.0 83 3291034.0 84 3291034.0 85 3291034.0 86 3291034.0 87 3291034.0 88 3291034.0 89 3291034.0 90 3291034.0 91 3291034.0 92 3291034.0 93 1710663.5 94 130293.0 95 130293.0 96 130293.0 97 130293.0 98 130293.0 99 130293.0 100 130293.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1816265246650538 2 0.17101936631382217 3 0.182490986499833 4 0.16560223494804466 5 0.16142563073500119 6 0.16775280761046152 7 0.15812895424643636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 7 1.22850999E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 0.03532059228857593 #Duplication Level Percentage of deduplicated Percentage of total 1 35.12046464040282 0.012404756125490156 2 16.970901264977424 0.011988445686998904 3 4.44900389066427 0.004714243575373221 4 3.6435686926829884 0.005147720170787018 5 1.4588182002317793 0.0025763161436770403 6 0.20280691755874747 4.2979562690372106E-4 7 0.8700013664527204 0.002151027448848634 8 0.4571861462131447 0.00129184683763038 9 0.8135499573008381 0.0025861559713390137 >10 4.000169259151198 0.04201445143535519 >50 4.900467271466267 0.12813818963856927 >100 13.810658310113125 1.1722160117675997 >500 2.65406354229209 0.6353714348105974 >1k 1.1305731486668371 0.71468683020344 >5k 0.1733471834750155 0.4293009755754065 >10k+ 9.344420208350801 96.83498179898201 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGA 934152 0.7603943049742722 No Hit TGGGGGA 283564 0.23081944982799854 No Hit GGGGAGA 225733 0.18374535155387706 No Hit GGGAGGA 224190 0.1824893585114436 No Hit CGGGGGA 180751 0.14713026468755047 No Hit AGGGGGA 179965 0.14649046525051052 No Hit GGGGGAA 178886 0.14561216551442127 No Hit GGGGTGA 171049 0.13923289301049965 No Hit GGGGGTA 168108 0.1368389360838653 No Hit NNNNNNN 164193 0.133652148811586 No Hit GTGGGGA 163475 0.13306770097978607 No Hit GGAGGGA 158523 0.1290368017275952 No Hit GCGGGGA 150643 0.12262252747330123 No Hit GGGCGGA 148898 0.12120210760353688 No Hit GGGGCGA 147934 0.12041741719983896 No Hit GGGTGGA 141479 0.11516308467300294 No Hit GAGGGGA 135445 0.11025144370213871 No Hit GGTGGGA 133095 0.10833855734457642 No Hit >>END_MODULE >>Adapter Content warn >>END_MODULE >>Kmer Content pass >>END_MODULE