FastQCFastQC Report
Mon 29 Sep 2014
Complete_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122850999
Sequences flagged as poor quality0
Sequence length7
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGA9341520.7603943049742722No Hit
TGGGGGA2835640.23081944982799854No Hit
GGGGAGA2257330.18374535155387706No Hit
GGGAGGA2241900.1824893585114436No Hit
CGGGGGA1807510.14713026468755047No Hit
AGGGGGA1799650.14649046525051052No Hit
GGGGGAA1788860.14561216551442127No Hit
GGGGTGA1710490.13923289301049965No Hit
GGGGGTA1681080.1368389360838653No Hit
NNNNNNN1641930.133652148811586No Hit
GTGGGGA1634750.13306770097978607No Hit
GGAGGGA1585230.1290368017275952No Hit
GCGGGGA1506430.12262252747330123No Hit
GGGCGGA1488980.12120210760353688No Hit
GGGGCGA1479340.12041741719983896No Hit
GGGTGGA1414790.11516308467300294No Hit
GAGGGGA1354450.11025144370213871No Hit
GGTGGGA1330950.10833855734457642No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)