##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Complete_Undetermined_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 122850999 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.311247155588863 27.0 15.0 33.0 15.0 33.0 2 27.490838434289003 33.0 27.0 33.0 15.0 33.0 3 27.79545296168084 33.0 27.0 33.0 15.0 33.0 4 32.728631917759174 37.0 33.0 37.0 22.0 37.0 5 33.1312180131315 37.0 33.0 37.0 22.0 37.0 6 33.778295771123524 37.0 33.0 37.0 27.0 37.0 7 17.87766785681572 2.0 2.0 37.0 2.0 37.0 8 24.213320674746814 15.0 15.0 37.0 15.0 37.0 9 31.24818630087005 33.0 33.0 37.0 15.0 37.0 10-11 33.68162630488662 37.0 33.0 37.0 27.0 37.0 12-13 33.9003099193357 37.0 33.0 37.0 27.0 37.0 14-15 35.70336960385646 40.0 33.0 40.0 27.0 40.0 16-17 35.689369339194386 40.0 33.0 40.0 27.0 40.0 18-19 35.5773558096992 40.0 33.0 40.0 27.0 40.0 20-21 35.55952325629847 40.0 33.0 40.0 27.0 40.0 22-23 35.38243063045829 40.0 33.0 40.0 27.0 40.0 24-25 35.47981990769159 40.0 33.0 40.0 27.0 40.0 26-27 35.49953391099408 40.0 33.0 40.0 27.0 40.0 28-29 35.45708328346601 40.0 33.0 40.0 27.0 40.0 30-31 35.39756444715602 40.0 33.0 40.0 27.0 40.0 32-33 35.321944337628054 40.0 33.0 40.0 27.0 40.0 34-35 35.282206537042484 40.0 33.0 40.0 27.0 40.0 36-37 35.250154050436336 40.0 33.0 40.0 27.0 40.0 38-39 35.16596337161247 40.0 33.0 40.0 22.0 40.0 40-41 35.030292745116384 40.0 33.0 40.0 22.0 40.0 42-43 34.940257661234 40.0 33.0 40.0 22.0 40.0 44-45 34.833760871574185 40.0 33.0 40.0 22.0 40.0 46-47 34.72839540360596 37.0 33.0 40.0 22.0 40.0 48-49 34.624753576484956 37.0 33.0 40.0 22.0 40.0 50-51 34.423497337616276 37.0 33.0 40.0 22.0 40.0 52-53 34.285174461625665 37.0 33.0 40.0 22.0 40.0 54-55 34.12078482568953 37.0 33.0 40.0 22.0 40.0 56-57 33.92827353809308 37.0 33.0 40.0 22.0 40.0 58-59 33.693061515112305 37.0 33.0 40.0 22.0 40.0 60-61 33.432652391373715 37.0 33.0 40.0 22.0 40.0 62-63 33.197670765379776 37.0 33.0 38.5 22.0 40.0 64-65 32.923430862780364 37.0 33.0 37.0 22.0 40.0 66-67 32.59756839665586 37.0 33.0 37.0 22.0 40.0 68-69 32.291359958741566 37.0 33.0 37.0 22.0 40.0 70-71 32.067416370785885 37.0 33.0 37.0 18.5 38.5 72-73 31.803233586240516 37.0 33.0 37.0 15.0 37.0 74-75 31.694637615441778 35.0 33.0 37.0 15.0 37.0 76-77 31.633060790169072 35.0 33.0 37.0 15.0 37.0 78-79 31.428139648257968 33.0 27.0 37.0 15.0 37.0 80-81 31.27902377090153 33.0 27.0 37.0 15.0 37.0 82-83 31.109206197826687 33.0 27.0 37.0 15.0 37.0 84-85 30.757118942923697 33.0 27.0 37.0 15.0 37.0 86-87 30.880638231521424 33.0 27.0 37.0 15.0 37.0 88-89 30.756757570200953 33.0 27.0 37.0 15.0 37.0 90-91 30.624612608970317 33.0 27.0 37.0 15.0 37.0 92-93 30.550012792325766 33.0 27.0 37.0 15.0 37.0 94-95 30.37048913619335 33.0 27.0 37.0 15.0 37.0 96-97 30.247215173235993 33.0 27.0 37.0 15.0 37.0 98-99 30.21990226143786 33.0 27.0 37.0 15.0 37.0 100-101 29.795013762973145 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 4.10905361652544 2201 2 2.1564883008739137 2201 3 2.5566376837550955 2201 4 2.092233864117574 2201 5 2.1457366768345523 2201 6 1.82146916305107 2201 7 17.797426161037873 2201 8 11.161460693082951 2201 9 3.962050117550895 2201 10-11 1.742251771833402 2201 12-13 1.4223074799396471 2201 14-15 1.808079339270698 2201 16-17 1.749642223735286 2201 18-19 1.7105537023191744 2201 20-21 1.7265565459498617 2201 22-23 1.720110313489208 2201 24-25 1.7041780426630666 2201 26-27 1.717920418023148 2201 28-29 1.728002121216484 2201 30-31 1.722113942202185 2201 32-33 1.759165357017416 2201 34-35 1.7694801380715646 2201 36-37 1.7739655771389664 2201 38-39 1.7530901060156836 2201 40-41 1.7515812361593888 2201 42-43 1.7959678179370613 2201 44-45 1.7655722661470818 2201 46-47 1.7807727400472686 2201 48-49 1.799401423631295 2201 50-51 1.8210150437829569 2201 52-53 1.7968606809806005 2201 54-55 1.7540747201181048 2201 56-57 1.7755538636046353 2201 58-59 1.786466203333255 2201 60-61 1.7905229284184117 2201 62-63 1.7973763508019402 2201 64-65 1.8396719514633162 2201 66-67 1.8489391330443965 2201 68-69 1.7878124252545078 2201 70-71 1.7824235252487952 2201 72-73 1.8092854069644062 2201 74-75 1.810924667524759 2201 76-77 1.8686471280559829 2201 78-79 1.8800871562184618 2201 80-81 1.933317621212268 2201 82-83 1.9088132391417432 2201 84-85 1.9250563997829033 2201 86-87 1.9045341146941794 2201 88-89 1.9104072038474627 2201 90-91 1.909851971120716 2201 92-93 1.9186538631107908 2201 94-95 1.9021113424686789 2201 96-97 1.8550041722143895 2201 98-99 1.9322000933742096 2201 100-101 1.8544704243492625 1101 1 0.2733258134497696 1101 2 0.12045312277176023 1101 3 -0.1645123037405014 1101 4 0.22044229073145516 1101 5 0.9477953379360642 1101 6 0.5845010335668519 1101 7 17.06248202978892 1101 8 10.101680032227208 1101 9 2.936264281695685 1101 10-11 0.7202131915537819 1101 12-13 0.3675325148416704 1101 14-15 0.5468029123953855 1101 16-17 0.48052928387112814 1101 18-19 0.4713249729436413 1101 20-21 0.48050581296891437 1101 22-23 0.4750672245729177 1101 24-25 0.48906931012397337 1101 26-27 0.490550613869317 1101 28-29 0.4894961722833173 1101 30-31 0.5007007447766014 1101 32-33 0.4461230944904031 1101 34-35 0.4792086477249953 1101 36-37 0.4829192875912014 1101 38-39 0.47456240421161766 1101 40-41 0.44489770519309246 1101 42-43 0.4789463804446825 1101 44-45 0.5090486149245876 1101 46-47 0.5092749320791228 1101 48-49 0.49510787062293105 1101 50-51 0.5390154555861315 1101 52-53 0.464529956202675 1101 54-55 0.4911945006386489 1101 56-57 0.5137477262560566 1101 58-59 0.5295003608633451 1101 60-61 0.5409064535492192 1101 62-63 0.5398123765828942 1101 64-65 0.5883481880229695 1101 66-67 0.5731689805979343 1101 68-69 0.5355782303518097 1101 70-71 0.5551379170948962 1101 72-73 0.6010449819715333 1101 74-75 0.5650299960745784 1101 76-77 0.5519867800239666 1101 78-79 0.5894607233467859 1101 80-81 0.6046809021247022 1101 82-83 0.5988993676930363 1101 84-85 0.6059929618892603 1101 86-87 0.6041444727042098 1101 88-89 0.6168855164626983 1101 90-91 0.6256895496870527 1101 92-93 0.6084385267669887 1101 94-95 0.6123980470008661 1101 96-97 0.6482412309689103 1101 98-99 0.6225262948325323 1101 100-101 0.5737824332738732 2202 1 0.6413140076558008 2202 2 0.4864822923368948 2202 3 -0.18582087393387425 2202 4 0.20016888464970606 2202 5 0.7315187988993586 2202 6 0.9090127741264382 2202 7 17.31711208995014 2202 8 10.554859215178709 2202 9 3.494669788025284 2202 10-11 1.1822226607000275 2202 12-13 0.850237073433739 2202 14-15 1.0865813168408849 2202 16-17 1.030247362490833 2202 18-19 1.047258465414906 2202 20-21 1.0461880540714432 2202 22-23 1.0313769642728516 2202 24-25 1.0280941417346767 2202 26-27 1.0542017366219838 2202 28-29 1.0407010359164701 2202 30-31 1.0167588844839344 2202 32-33 1.017429902807514 2202 34-35 1.0141393589790013 2202 36-37 1.0640268506452344 2202 38-39 1.0314873882539857 2202 40-41 1.0291622322944107 2202 42-43 1.0146139324284391 2202 44-45 0.9955257634389483 2202 46-47 1.00335032562095 2202 48-49 1.0361620391556627 2202 50-51 1.0484890719917885 2202 52-53 1.0570864384721403 2202 54-55 1.047700581352622 2202 56-57 1.0431216634563256 2202 58-59 1.0516133380716113 2202 60-61 1.0555330205307811 2202 62-63 1.0483373347267317 2202 64-65 1.0699687444486798 2202 66-67 1.0625829943784737 2202 68-69 1.0666586577837407 2202 70-71 1.076845035153326 2202 72-73 1.0830464434205496 2202 74-75 1.0966541301951693 2202 76-77 1.1239474682252535 2202 78-79 1.1192515244335972 2202 80-81 1.1604074279976118 2202 82-83 1.1730393164359398 2202 84-85 1.178876458254443 2202 86-87 1.1513887676836845 2202 88-89 1.1811776822451137 2202 90-91 1.192687466850714 2202 92-93 1.2058089985759395 2202 94-95 1.2224397834862302 2202 96-97 1.2578322217156632 2202 98-99 1.2196422472871227 2202 100-101 1.1812036291495893 1102 1 -0.5174755034351257 1102 2 -0.12913341585598204 1102 3 1.5816822371430845 1102 4 1.348598050191633 1102 5 1.273656707298322 1102 6 0.901730737073656 1102 7 -16.374127169190356 1102 8 -9.763415353631407 1102 9 -1.8953142446103364 1102 10-11 0.1117247195483344 1102 12-13 0.33195859578303555 1102 14-15 0.37074778494987726 1102 16-17 0.3847319313313946 1102 18-19 0.3803632088156803 1102 20-21 0.3847104458973263 1102 22-23 0.430833767474617 1102 24-25 0.4112374919140649 1102 26-27 0.440056783623227 1102 28-29 0.4413750218866781 1102 30-31 0.41468149436589385 1102 32-33 0.4337497009962803 1102 34-35 0.4361726668259678 1102 36-37 0.4367022509788896 1102 38-39 0.4517239085132516 1102 40-41 0.45551528431814603 1102 42-43 0.4613070041413678 1102 44-45 0.44220360983108975 1102 46-47 0.44466233181408654 1102 48-49 0.4334861828616994 1102 50-51 0.4399024758503458 1102 52-53 0.4610236016072662 1102 54-55 0.4631174034261676 1102 56-57 0.41561148835128137 1102 58-59 0.42053587180934215 1102 60-61 0.4051698236441865 1102 62-63 0.3768268844283327 1102 64-65 0.3775891487100722 1102 66-67 0.3625473066657179 1102 68-69 0.36971835973880474 1102 70-71 0.32505917978252796 1102 72-73 0.3329574369485755 1102 74-75 0.33867335631267537 1102 76-77 0.30608833700949845 1102 78-79 0.31890040921561535 1102 80-81 0.3137623828924987 1102 82-83 0.3373824773441356 1102 84-85 0.3539622199083965 1102 86-87 0.30130474699101484 1102 88-89 0.3172893652075288 1102 90-91 0.33633463132745334 1102 92-93 0.3409239822674337 1102 94-95 0.3664724089394156 1102 96-97 0.39785867334828495 1102 98-99 0.3898061391851151 1102 100-101 0.38000223826775326 2203 1 -1.6973252322880654 2203 2 -0.6540409728519521 2203 3 0.9620305954933421 2203 4 0.9251893842134322 2203 5 0.9568257976863777 2203 6 0.8151073373160997 2203 7 -16.374127169190356 2203 8 -9.754938074297879 2203 9 -2.0290058796433854 2203 10-11 0.11400424258376773 2203 12-13 0.3510010773785055 2203 14-15 0.4040003606033835 2203 16-17 0.4154168048987614 2203 18-19 0.40891656530996556 2203 20-21 0.4451571412854278 2203 22-23 0.46929618521922123 2203 24-25 0.45504846767476437 2203 26-27 0.43750172955527233 2203 28-29 0.4675728563508059 2203 30-31 0.4529237847764591 2203 32-33 0.46246702981008525 2203 34-35 0.5021095051564757 2203 36-37 0.4704365394832095 2203 38-39 0.5009188932559994 2203 40-41 0.49871281916937704 2203 42-43 0.49752272393575936 2203 44-45 0.5175290051427055 2203 46-47 0.5084377856961453 2203 48-49 0.4931353208453402 2203 50-51 0.5063769657514925 2203 52-53 0.5085291624977231 2203 54-55 0.5333430310042502 2203 56-57 0.48935635016723467 2203 58-59 0.44690814838055815 2203 60-61 0.4654454192085069 2203 62-63 0.43380010569514127 2203 64-65 0.42365839858157983 2203 66-67 0.3843820043913695 2203 68-69 0.3986420393660737 2203 70-71 0.35962592547672045 2203 72-73 0.36260501114667676 2203 74-75 0.3623643747100047 2203 76-77 0.35189942780280603 2203 78-79 0.3490634195315714 2203 80-81 0.38521414958643163 2203 82-83 0.3911833163945566 2203 84-85 0.39413432519528513 2203 86-87 0.37538610017529805 2203 88-89 0.36089798931422123 2203 90-91 0.37891112792178205 2203 92-93 0.3681722170547026 2203 94-95 0.38511086532493266 2203 96-97 0.4857705732830091 2203 98-99 0.39880489371003947 2203 100-101 0.39703762236129236 1103 1 3.9980783478966906 1103 2 1.8545285216655962 1103 3 2.418855992650734 1103 4 1.920502073075184 1103 5 1.9380232932325754 1103 6 1.5414224289828837 1103 7 -16.374127169190356 1103 8 -9.714818489159935 1103 9 -1.866785024692792 1103 10-11 0.6019649416688324 1103 12-13 0.9536392393848274 1103 14-15 1.2047774793679835 1103 16-17 1.2434667546849951 1103 18-19 1.2423208893756765 1103 20-21 1.2452654112583588 1103 22-23 1.2567056753404913 1103 24-25 1.266982059167205 1103 26-27 1.2587168301079288 1103 28-29 1.2566032648589172 1103 30-31 1.2754774243644817 1103 32-33 1.3008939250296976 1103 34-35 1.3077841611709786 1103 36-37 1.3106341631028684 1103 38-39 1.2567518288545259 1103 40-41 1.2996307175521906 1103 42-43 1.2785444226422413 1103 44-45 1.298249963752859 1103 46-47 1.290579907146146 1103 48-49 1.3070994807096739 1103 50-51 1.2998974614047611 1103 52-53 1.2897661781436156 1103 54-55 1.2871309782755418 1103 56-57 1.245882033225449 1103 58-59 1.2405263596967728 1103 60-61 1.2314268131727175 1103 62-63 1.178618282604269 1103 64-65 1.1330071849505856 1103 66-67 1.1611571394993447 1103 68-69 1.1939197912538262 1103 70-71 1.1403508202136408 1103 72-73 1.1321475387572164 1103 74-75 1.2080519546695072 1103 76-77 1.2118873417094136 1103 78-79 1.265270470735718 1103 80-81 1.302476135424854 1103 82-83 1.3232075656972953 1103 84-85 1.3170691935480718 1103 86-87 1.3416491996292024 1103 88-89 1.3386820388632472 1103 90-91 1.3789158764828997 1103 92-93 1.399968617944019 1103 94-95 1.409135293838741 1103 96-97 1.4197885877265612 1103 98-99 1.4056433425831223 1103 100-101 1.3591844047834165 2204 1 0.5380466301147031 2204 2 0.2807468722462225 2204 3 1.987952237038055 2204 4 1.8105473285958666 2204 5 1.5549355463686965 2204 6 1.1578939732476172 2204 7 17.513397188697194 2204 8 10.606409396680235 2204 9 3.5127982480444544 2204 10-11 1.1713676919342078 2204 12-13 0.8045771159291206 2204 14-15 0.990101857200294 2204 16-17 0.9468077193093407 2204 18-19 0.9186110600560511 2204 20-21 0.9615184442794202 2204 22-23 0.9405667354241274 2204 24-25 0.9495513639353774 2204 26-27 0.9403026735680342 2204 28-29 0.9266355066800926 2204 30-31 0.9538991260820424 2204 32-33 0.9597624709989461 2204 34-35 0.9379024854524189 2204 36-37 0.9288403537376695 2204 38-39 0.9352901129204128 2204 40-41 0.9760666479269204 2204 42-43 0.9439503764566126 2204 44-45 0.9403585589963726 2204 46-47 0.9196938218658204 2204 48-49 0.905224164122906 2204 50-51 0.9552956930149676 2204 52-53 0.9377544200555832 2204 54-55 0.9290336119885438 2204 56-57 0.9069390485697753 2204 58-59 0.8867825882685452 2204 60-61 0.9110513194730601 2204 62-63 0.8882173695448259 2204 64-65 0.9426564140398881 2204 66-67 0.9689992474460851 2204 68-69 0.9607315394566811 2204 70-71 0.9414951676225201 2204 72-73 0.9742053746204391 2204 74-75 0.9602187675545579 2204 76-77 1.046535806907201 2204 78-79 1.0617109673867198 2204 80-81 1.0653053967561235 2204 82-83 1.0927879180940465 2204 84-85 1.040479498518316 2204 86-87 1.0426160267807916 2204 88-89 1.0480860649684018 2204 90-91 1.1114911747684282 2204 92-93 1.0960812497122774 2204 94-95 1.1064342196858163 2204 96-97 1.1583442072042054 2204 98-99 1.0837032968774984 2204 100-101 1.1033027239722877 2205 1 -1.488979294330072 2205 2 -0.5711580935245522 2205 3 1.0302838783323622 2205 4 0.8987369449970259 2205 5 0.9730445898595619 2205 6 0.673052236853998 2205 7 -16.374127169190356 2205 8 -9.764481958318287 2205 9 -2.5171578579270246 2205 10-11 -0.18981600097290396 2205 12-13 0.1779725247100714 2205 14-15 0.0869164065231871 2205 16-17 0.1500291641279503 2205 18-19 0.17342477589144778 2205 20-21 0.15918856247161983 2205 22-23 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1213 26-27 -3.769585955764043 1213 28-29 -3.893517833640871 1213 30-31 -3.847673366543763 1213 32-33 -3.8396395844063775 1213 34-35 -3.8422228849140865 1213 36-37 -3.8347495502761504 1213 38-39 -3.8810021652803 1213 40-41 -3.8848597426617744 1213 42-43 -3.9023665180021396 1213 44-45 -3.868847601959736 1213 46-47 -3.815696454954111 1213 48-49 -3.863803240788659 1213 50-51 -3.889377704334116 1213 52-53 -3.8953968017361085 1213 54-55 -3.819477656468159 1213 56-57 -3.8028798924157385 1213 58-59 -3.8000496053979873 1213 60-61 -3.828395399252649 1213 62-63 -3.7784058093262303 1213 64-65 -3.8230218041376176 1213 66-67 -3.858039056956663 1213 68-69 -3.852569188606573 1213 70-71 -3.865333568889614 1213 72-73 -3.868994914027862 1213 74-75 -3.9154496191597765 1213 76-77 -4.133546763336859 1213 78-79 -4.180593844897555 1213 80-81 -4.2391360552374735 1213 82-83 -4.308779926303146 1213 84-85 -4.273023051941852 1213 86-87 -4.374539862861965 1213 88-89 -4.418010710957603 1213 90-91 -4.452031798876202 1213 92-93 -4.515504251119573 1213 94-95 -4.5136659970406185 1213 96-97 -4.527325432336841 1213 98-99 -4.681805591493099 1213 100-101 -4.498214305955781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38920.0 3 108971.0 4 27192.0 5 13848.0 6 9603.0 7 8885.0 8 11840.0 9 37901.0 10 225821.0 11 822885.0 12 1555649.0 13 1817907.0 14 1619171.0 15 1335120.0 16 1140204.0 17 1026665.0 18 965465.0 19 945649.0 20 952095.0 21 973802.0 22 1006372.0 23 1079435.0 24 1160383.0 25 1246485.0 26 1386060.0 27 1658173.0 28 2226539.0 29 3211457.0 30 3878155.0 31 4132728.0 32 3095293.0 33 4049618.0 34 6280611.0 35 1.0840936E7 36 2.0182702E7 37 3.2982474E7 38 1.077927E7 39 16715.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.8361331882705 20.7248317260821 31.616983623744066 22.82205146190333 2 22.60024266112235 9.076184268547365 18.70192317127405 49.62164989905624 3 16.034542263664832 33.22190269367291 27.39108486448945 23.352470178172805 4 18.576686995074503 22.700113863978597 22.23379732081528 36.489401820131626 5 33.58914931556946 24.89729378303754 22.327343574426127 19.18621332696687 6 25.67433106770567 33.63158216608888 33.56966976938115 7.124416996824302 7 0.5257080472291266 0.5732961802414898 98.22012434692563 0.6808714256037552 8 41.820799189681544 12.80530925231631 9.243761669113368 36.13012988888878 9 26.175257138248835 10.541637715477314 27.05737991755388 36.225725228719966 10-11 27.996242272635634 13.794372965013002 17.13315653033071 41.076228232020654 12-13 27.18427958027037 23.371244065496242 23.833838323073326 25.61063803116006 14-15 21.296575722488488 23.797606107468958 34.20722806178975 20.698590108252805 16-17 20.19977572282694 27.562372615551222 31.357875225100447 20.879976436521392 18-19 23.253410538901512 27.856378242331786 26.55443767848532 22.33577354028138 20-21 21.633422099077023 25.71958105853527 28.810540937841157 23.836455904546554 22-23 20.51106358627525 29.811742506656287 27.746294276705335 21.930899630363125 24-25 21.329105103493124 28.69584218016008 29.105490130444135 20.869562585902663 26-27 21.042767666160476 28.192788796042993 29.697330895852815 21.067112641943716 28-29 21.72644962053087 29.915482626800838 27.64084077160701 20.717226981061277 30-31 21.509389171491122 27.880988892530684 28.66863294328042 21.940988992697772 32-33 21.693223855412214 27.73626090416595 29.068838739138357 21.50167650128348 34-35 21.824982355106357 27.783846835817684 29.18069021639923 21.210480592676728 36-37 22.821616179375404 25.688798643761906 29.73696061810408 21.75262455875861 38-39 21.06373947080895 28.65276922740638 26.688554122138125 23.59493717964654 40-41 20.618079528935922 29.6696513303485 26.964352839009592 22.747916301705988 42-43 21.95963184474147 27.0002501615648 26.52208228844663 24.5180357052471 44-45 21.96337073139771 28.163135659124062 26.741187941988994 23.13230566748923 46-47 20.21088630887098 29.71719539305615 27.722994989013568 22.348923309059302 48-49 20.713950488924997 27.521818424036976 27.05884604549803 24.705385041539994 50-51 20.598124251129583 28.555699990120136 26.48538080814627 24.360794950604014 52-53 19.84675513485256 28.08555117367514 28.335861005688994 23.731832685783306 54-55 20.831951524077745 25.71827178328362 29.20643442964202 24.243342262996613 56-57 21.323727176961746 25.525746791335273 30.075105400080517 23.075420631622464 58-59 22.31440749419145 26.521948450931443 26.28520941007212 24.878434644804987 60-61 23.79179894946455 25.507284918357897 28.480163046947744 22.22075308522981 62-63 25.55039742003621 24.564571475454418 27.520795885692568 22.364235218816805 64-65 21.99540904689171 26.03505020105896 27.768938519378526 24.200602232670803 66-67 22.160790170665184 29.064778585196237 26.63315104753688 22.141280196601702 68-69 23.056081978416955 28.695306947059674 25.742632497641843 22.505978576881528 70-71 21.85268595845649 28.052270910517414 25.731315469311877 24.363727661714215 72-73 24.25591053970624 24.403657034716204 26.868627827164175 24.47180459841338 74-75 21.14225031822654 26.33648500796233 24.858835471173375 27.66242920263775 76-77 23.52971044430425 27.055580410544618 24.640154972810542 24.774554172340586 78-79 23.357521644576824 28.790398746517614 25.288544475686038 22.563535133219528 80-81 25.513659263429417 26.354971077201476 25.260360228055447 22.871009431313663 82-83 25.6536945562647 27.470734992569035 26.21198938281707 20.663581068349192 84-85 23.770356257126384 24.241718312551043 28.977045793445168 23.010879636877412 86-87 22.16793478323132 27.38171242293344 26.179721816067442 24.270630977767794 88-89 22.050244170998326 28.26826251943006 25.879588532942062 23.801904776629556 90-91 22.17916238558479 26.318640909890377 27.918600095971918 23.583596608552913 92-93 23.295798837618804 27.134069957647462 25.974221383414154 23.595909821319587 94-95 21.94107835458547 29.69198941914632 25.442349510373123 22.924582715895085 96-97 23.15265716244917 29.466060564704343 25.44599578849468 21.93528648435181 98-99 23.0895289103974 27.955172506696822 25.504906028585843 23.450392554319933 100-101 22.836792183376957 27.941028147106177 27.782560993577494 21.43961867593937 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 526.0 1 4397.0 2 28331.0 3 82662.0 4 83184.0 5 39283.0 6 23717.5 7 16276.5 8 12938.5 9 10710.0 10 9444.0 11 8904.0 12 8561.5 13 8435.0 14 8609.5 15 9376.0 16 11233.0 17 16940.5 18 45499.0 19 108404.5 20 108770.0 21 77506.5 22 87289.0 23 101723.5 24 134474.0 25 166138.5 26 195795.0 27 257759.5 28 326728.5 29 413632.5 30 588562.5 31 719197.5 32 807985.5 33 944341.5 34 1143820.5 35 1415998.5 36 1668353.0 37 2003807.0 38 2529833.0 39 3140373.0 40 3676568.0 41 4073340.5 42 4707675.5 43 5779659.0 44 6921203.0 45 8160355.5 46 9114603.5 47 9679384.5 48 8715356.5 49 6672982.5 50 5569731.5 51 4969238.5 52 4432589.5 53 3992302.5 54 3435893.5 55 2807653.5 56 2410401.5 57 2378521.5 58 2111279.0 59 1529195.5 60 1076424.5 61 747518.5 62 582107.0 63 421725.5 64 321273.5 65 254271.0 66 205914.5 67 167408.0 68 134972.0 69 106357.5 70 81449.0 71 62208.5 72 47880.5 73 35960.5 74 26345.5 75 19338.5 76 13942.5 77 9794.5 78 6948.0 79 4773.0 80 3273.0 81 2356.0 82 1724.0 83 1293.5 84 957.0 85 708.0 86 541.5 87 420.5 88 362.0 89 298.0 90 240.5 91 239.5 92 228.0 93 217.5 94 272.5 95 347.0 96 370.0 97 360.0 98 476.0 99 1198.5 100 1104.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.058623047908629544 2 0.039452670629076446 3 0.005834710387662375 4 0.03290978529201866 5 0.05021855784827602 6 0.06704951581224015 7 53.093005780115796 8 0.11856720839526914 9 0.15508461595823084 10-11 0.17644911458961762 12-13 0.18804568288451606 14-15 0.19558123414201944 16-17 0.20025193283125028 18-19 0.21038453256696757 20-21 0.20902068529373538 22-23 0.20393851253907996 24-25 0.2079653418202973 26-27 0.2158146878398604 28-29 0.22289928631349593 30-31 0.20824983279134748 32-33 0.21878413866215285 34-35 0.2136055075954246 36-37 0.20478588049577034 38-39 0.1988091281211315 40-41 0.1959756143293552 42-43 0.18904404676432462 44-45 0.19551896358612433 46-47 0.2007277124380568 48-49 0.2001159958007342 50-51 0.19372817635776818 52-53 0.1748341501073182 54-55 0.17818495720983107 56-57 0.18336236728526725 58-59 0.171669340678296 60-61 0.16095677007884973 62-63 0.15217784268893084 64-65 0.17042026658651754 66-67 0.1718862701311855 68-69 0.17875271816063945 70-71 0.18857762809075732 72-73 0.18211044421380734 74-75 0.1891535289835128 76-77 0.18155041620784865 78-79 0.18570056560956416 80-81 0.17574867258507193 82-83 0.1795195006920538 84-85 0.16940765780830158 86-87 0.17200796226329426 88-89 0.17919024004029466 90-91 0.1920741401541228 92-93 0.17205313894110053 94-95 0.17082075173031355 96-97 0.1560988527248362 98-99 0.16197873979030483 100-101 0.16744593179905684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.22850999E8 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.477103312008964 #Duplication Level Percentage of deduplicated Percentage of total 1 72.8416977932734 18.55795460101362 2 12.850766690895998 6.548006212449618 3 4.1042840203117565 3.136958040219303 4 2.10684045685148 2.1470476792450124 5 1.4607972816154453 1.8608441630809276 6 0.8349954472170246 1.2763959164283156 7 0.5886436106105868 1.0497853857035928 8 0.4214310531850141 0.8589473984706679 9 0.431852888309622 0.9902124585948336 >10 3.6115518067282646 19.51861788144775 >50 0.42264237744403416 7.426034951078085 >100 0.2698297677112608 13.623893973253795 >500 0.032590158717947654 5.707192275198195 >1k 0.019727587967526758 9.952246272421277 >5k 0.0016465248638334435 2.8296074890487515 >10k+ 7.025342967894305E-4 4.516255302346375 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTTGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 303140 0.24675420018358987 No Hit TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 201670 0.16415820924663382 No Hit CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 179478 0.14609405007768803 No Hit ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 146825 0.1195146976379085 No Hit TCGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA 134932 0.10983386468025383 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.953936141780989E-6 0.0 0.0 2 8.953936141780989E-6 0.0 0.0 3 8.953936141780989E-6 0.0 0.0 4 1.2535510598493384E-4 0.0 8.139941947073626E-7 5 1.30239071153178E-4 0.0 8.139941947073626E-7 6 1.5140292021556943E-4 1.6279883894147252E-6 8.139941947073626E-7 7 1.6849679830442405E-4 1.6279883894147252E-6 8.139941947073626E-7 8 0.002131036801743875 2.4419825841220875E-6 8.139941947073626E-7 9 0.0022327860760822955 2.4419825841220875E-6 2.4419825841220875E-6 10-11 0.0027484513984294095 3.2559767788294503E-6 2.4419825841220875E-6 12-13 0.018532612827999874 3.2559767788294503E-6 2.4419825841220875E-6 14-15 0.30947774384805776 3.2559767788294503E-6 4.883965168244175E-6 16-17 0.30965763656508805 4.069970973536813E-6 6.918950655012581E-6 18-19 0.3098053765114275 7.325947752366263E-6 8.139941947073625E-6 20-21 0.3099510814722801 7.325947752366263E-6 8.139941947073625E-6 22-23 0.3100528307466185 7.732944849719945E-6 8.546939044427306E-6 24-25 0.310123648241558 8.953936141780987E-6 8.953936141780989E-6 26-27 0.3101997566987632 1.0581924531195713E-5 4.9653645877149114E-5 28-29 0.31026487623433974 1.0581924531195713E-5 8.791137302839515E-5 30-31 0.31032551880184545 1.0581924531195713E-5 8.872536722310251E-5 32-33 0.3105501811995847 1.3023907115317801E-5 8.91323643204562E-5 34-35 0.3112864389486975 1.3023907115317801E-5 9.11673498072246E-5 36-37 0.3129677439578656 1.3837901310025163E-5 9.320233529399302E-5 38-39 0.3156518898149131 1.4244898407378844E-5 9.401632948870038E-5 40-41 0.3207287716073029 1.546588969943989E-5 9.442332658605406E-5 42-43 0.3301275555764915 1.587288679679357E-5 9.483032368340774E-5 44-45 0.3453020353542261 1.668688099150093E-5 9.523732078076142E-5 46-47 0.3640088429398934 1.7093878088854612E-5 9.645831207282246E-5 48-49 0.39084094057712954 1.872186647826934E-5 9.686530917017614E-5 50-51 0.43188619084815094 1.912886357562302E-5 9.930729175429823E-5 52-53 0.4811352816105305 2.0349854867684062E-5 1.0093528014371295E-4 54-55 0.5460203868590437 2.4419825841220876E-5 1.0093528014371295E-4 56-57 0.6178073488844809 2.5233820035928238E-5 1.0174927433842031E-4 58-59 0.7015942133282937 2.6047814230635603E-5 1.0174927433842031E-4 60-61 0.8012653604876262 2.7675802620050326E-5 1.0581924531195713E-4 62-63 0.9233014051436408 2.9303791009465052E-5 1.0785423079872554E-4 64-65 1.0705415590474767 2.9303791009465052E-5 1.0948221918814026E-4 66-67 1.2467725231929128 2.9303791009465052E-5 1.107032104802013E-4 68-69 1.4370741909880604 2.9303791009465052E-5 1.107032104802013E-4 70-71 1.649546618664452 2.9710788106818733E-5 1.107032104802013E-4 72-73 1.9015746872355512 3.0117785204172414E-5 1.1314519306432339E-4 74-75 2.1987297799670316 3.0117785204172414E-5 1.1640116984315285E-4 76-77 2.539877188951471 3.0117785204172414E-5 1.1640116984315285E-4 78-79 2.924289610375899 3.133877649623346E-5 1.1640116984315285E-4 80-81 3.3070117728550175 3.174577359358714E-5 1.1640116984315285E-4 82-83 3.7028827905583412 3.174577359358714E-5 1.1843615532992125E-4 84-85 4.13794681474263 3.25597677882945E-5 1.2128513501139703E-4 86-87 4.606501002079764 3.25597677882945E-5 1.2372711759551911E-4 88-89 5.105290189785107 3.25597677882945E-5 1.2372711759551911E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCAGAT 635700 0.0 183.69855 1 GATCAGT 264765 0.0 177.6601 1 ACAGTGT 648790 0.0 176.29393 1 CGATGTT 650960 0.0 175.88242 1 GACCATG 301285 0.0 170.8878 2 CTCAGTC 183775 0.0 167.8851 2 CAGTGTG 325950 0.0 166.01073 2 TCAGATG 276555 0.0 163.67726 2 ACTTGAT 603620 0.0 163.21771 1 AGTGTGC 155115 0.0 163.09938 3 TCGCTTT 359870 0.0 162.0668 1 GTGGCCT 290255 0.0 161.6262 1 CTCAGTG 219335 0.0 161.54831 2 GCTCAGT 281760 0.0 160.73918 1 TCTCAGT 240900 0.0 160.66345 1 GCCAATT 455935 0.0 159.44006 1 TCAGTCG 75115 0.0 158.75931 3 ATCAGTC 134785 0.0 152.88509 2 TGACCAT 720505 0.0 151.06786 1 TGGCCTG 203105 0.0 150.10838 2 >>END_MODULE