FastQCFastQC Report
Mon 29 Sep 2014
Complete_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122850999
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA3031400.24675420018358987No Hit
TGACCATCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA2016700.16415820924663382No Hit
CGATGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1794780.14609405007768803No Hit
ACAGTGTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1468250.1195146976379085No Hit
TCGCTTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCA1349320.10983386468025383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT6357000.0183.698551
GATCAGT2647650.0177.66011
ACAGTGT6487900.0176.293931
CGATGTT6509600.0175.882421
GACCATG3012850.0170.88782
CTCAGTC1837750.0167.88512
CAGTGTG3259500.0166.010732
TCAGATG2765550.0163.677262
ACTTGAT6036200.0163.217711
AGTGTGC1551150.0163.099383
TCGCTTT3598700.0162.06681
GTGGCCT2902550.0161.62621
CTCAGTG2193350.0161.548312
GCTCAGT2817600.0160.739181
TCTCAGT2409000.0160.663451
GCCAATT4559350.0159.440061
TCAGTCG751150.0158.759313
ATCAGTC1347850.0152.885092
TGACCAT7205050.0151.067861
TGGCCTG2031050.0150.108382
Produced by FastQC (version 0.11.2)