FastQCFastQC Report
Fri 19 Sep 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758919
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA42700.040.264661
ATCGGAA42700.040.2491262
ACACGTC38300.039.2498713
TCGGAAG44150.038.7244953
CGGAAGA43850.038.529624
CACACGT40400.038.0404512
CACGTCT39000.038.0283814
CGTCTGA38600.037.58687616
ACGTCTG39000.037.56882515
GCACACG41650.037.16772511
CGCAATA4250.036.07222733
AGCACAC47150.033.33659410
AGAGCAC48900.033.2221538
CTGAACT46750.029.67810419
GAAGAGC56450.029.4923256
GAGCACA55450.029.1542249
TGAACTC48150.028.41261320
GAACTCC46750.028.16204321
AAGAGCA59600.027.670777
TCTGAAC52100.027.1838418
Produced by FastQC (version 0.11.2)