FastQCFastQC Report
Fri 19 Sep 2014
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4810677
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA28950.038.8346062
GATCGGA29150.038.652871
ACACGTC25850.037.78508413
CGTCTGA25550.037.35588516
ACGTCTG26050.037.0671415
TCGGAAG30400.036.614683
CGGAAGA30700.036.1837044
TTCGGTA6650.035.8558325
GAATTCG6650.035.7324822
CACGTCT27550.035.45351814
GCACACG28900.034.95820611
CACACGT28650.034.95118312
ATTCGGT6850.034.75114424
AATTCGG6850.034.72394623
TATTCGT5700.034.5141831
AGCCGAT4700.031.3997518
CCGATGA4800.030.79875420
TAAGCCG4900.030.56333716
CGTTACG300.00498540830.19858735
GATGACG4900.030.19442622
Produced by FastQC (version 0.11.2)