FastQCFastQC Report
Fri 19 Sep 2014
SV65_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV65_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20767563
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1127580.5429524879736732TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG135150.039.55782743
CTCGTAT135750.039.28334442
TCTCGTA136350.039.1104841
CGTATGC137400.038.8782344
ATCTCGT141500.037.7505440
AATCTCG142400.037.58959639
GTATGCC148000.036.1849345
GTAATCT155250.034.4928137
ATCGGAA161900.034.2306752
GATCGGA164300.033.8393061
ACACGTC162150.033.59289613
CGTCTGA163600.033.32266616
ACGTCTG166150.032.83832615
TGTAATC167750.032.0556836
TAATCTC168400.031.87950338
CACGTCT171650.031.85166214
TCGGAAG176550.031.492193
CGGAAGA182300.030.3496744
CACACGT181300.030.28040312
CTTGTAA181250.029.69291334
Produced by FastQC (version 0.11.2)