FastQCFastQC Report
Fri 19 Sep 2014
SV64_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV64_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20181654
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC9357094.636433663960347TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1012950.044.002292
GATCGGA1013950.043.951221
ACACGTC1008100.043.62638513
CGTCTGA1010300.043.3955416
ACGTCTG1011900.043.3580515
TCGGAAG1027350.043.3524553
TATCTCG998200.043.31148539
CTCGTAT999050.043.24942
CGCCAAT999900.043.2303533
TCGTATG1001750.043.21800243
CACGTCT1017850.043.16206714
TCTCGTA1001450.043.1453641
CGGAAGA1030250.043.1200524
CGTATGC1004300.043.1000544
ATCTCGT1003050.043.09437640
CACACGT1022400.043.07121312
GTATGCC1004400.043.0667545
ACGCCAA1009050.042.92976432
GCCAATA1008800.042.83111234
AGTCACG1013150.042.7804628