FastQCFastQC Report
Fri 19 Sep 2014
SV63_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV63_ACAGTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21143830
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC597450.28256470090801905TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG77700.036.7486643
CTCGTAT79250.035.97313342
CGTATGC81800.034.9344944
TCTCGTA81650.034.9157541
ATCTCGT87100.032.8084340
GTATGCC91550.031.28773145
GATCTCG93350.030.4419539
ATCGGAA101300.029.1197742
GATCGGA104550.028.2461761
ACACGTC105300.027.75574913
CGTCTGA107100.027.26825716
ACGTCTG110050.026.45552415
AGTGATC110950.025.7338336
CACGTCT116200.025.17151614
GTGATCT115300.024.99788537
TCGGAAG122350.024.330423
CACACGT121900.024.16062212
CGGAAGA125050.023.7864234
TGATCTC125700.022.80434438
GTCACAC133250.021.62977629
Produced by FastQC (version 0.11.2)