FastQCFastQC Report
Fri 19 Sep 2014
SV62_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV62_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22986449
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC759650.33047731730986374TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT95150.037.0556742
TCGTATG96050.036.7553943
TCTCGTA96650.036.5038541
CGTATGC98300.035.98393244
AATCTCG101050.034.8239439
ATCTCGT102400.034.54196540
GATCGGA116400.032.1016431
ATCGGAA117950.031.685772
GTATGCC113050.031.36867745
ACACGTC121300.030.06727413
CGTCTGA122300.029.65572216
GACCAAT123900.028.52822935
ACGTCTG128150.028.3546215
TCGGAAG134950.027.7775973
ACCAATC127400.027.67384536
CACGTCT132450.027.50215514
CGGAAGA141600.026.5041034
CCAATCT139600.025.44914837
CACACGT147400.025.0943612
CAATCTC140900.025.07062738
Produced by FastQC (version 0.11.2)