FastQCFastQC Report
Fri 19 Sep 2014
SV61_CGATGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV61_CGATGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20618658
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC710830.3447508562390433TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT83900.038.37786542
TCGTATG84700.038.14840343
CGTATGC86400.037.45051644
TCTCGTA85950.037.43624541
TATCTCG85950.037.35589639
CGATGTA86750.037.1142534
ACCGATG91050.035.41088532
CCGATGT91650.035.2036133
ATCTCGT92350.034.98804540
CACCGAT92300.034.98007631
ATCGGAA100150.033.745422
ACACGTC98600.033.63513613
GATCGGA101100.033.3276331
GTATGCC97850.033.11428545
CGTCTGA103150.032.1512416
TCACCGA101200.032.01492730
GATGTAT103300.031.42935635
ACGTCTG105850.031.33113315
ATGTATC105300.030.66147436
CACGTCT109450.030.25970514
Produced by FastQC (version 0.11.2)