FastQCFastQC Report
Fri 19 Sep 2014
SV60_CAGATC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV60_CAGATC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15893026
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC5687133.5783808571130504TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA620800.044.070641
ATCGGAA623450.043.8972782
ACACGTC617850.043.7643413
CGTCTGA617200.043.6901316
ACGTCTG618950.043.59931615
TCGGAAG627400.043.581323
CGGAAGA627650.043.473384
CACGTCT623800.043.32164814
CTCGTAT615050.043.23398242
TCTCGTA614200.043.20589441
GCACACG628750.043.10225711
TCGTATG619100.043.01657543
CGTATGC619100.043.0098544
CACACGT630650.042.9545612
CATCTCG617100.042.93978539
ATCTCGT619100.042.860340
GTATGCC624950.042.5748445
GATCATC631200.041.96539736
GAACTCC646600.041.54471221
CCAGATC647250.041.24805533
Produced by FastQC (version 0.11.2)