FastQCFastQC Report
Fri 19 Sep 2014
SV55_CAGATC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV55_CAGATC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18638603
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2490461.3361838330909241TruSeq Adapter, Index 7 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA1358290.7287509691579352No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC1057420.5673279268837905No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC238930.1281909379152504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA274200.044.2746351
ATCGGAA276400.043.8545232
ACACGTC277400.043.25539413
CGGAAGA281250.043.0742764
TCGGAAG281250.043.0662773
CGTCTGA278850.042.9901316
ACGTCTG279450.042.9300315
TAAGCCG150950.042.8081516
TCTCGTA274750.042.71561441
CTCGTAT275100.042.71034642
AGCCGAT152050.042.4836618
TCGTATG277900.042.39336843
GGCGGTC142400.042.3289938
CGTATGC279250.042.24493444
AATTCGG142750.042.24096723
CCGATGA152800.042.2309620
TATTCGT115250.042.1621971
CACGTCT285100.042.1187214
GCGGTCT143250.042.10923439
CGATGAC153650.042.02662321