FastQCFastQC Report
Fri 19 Sep 2014
SV54_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV54_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30232179
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC5247781.735825922438472TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA590400.043.0980071
ATCGGAA592000.042.9496272
CGCCAAT577150.042.73818233
CTCGTAT575750.042.6600642
TCTCGTA574650.042.6515241
TATCTCG576350.042.56725339
CGTCTGA590400.042.50684716
ACACGTC591100.042.5021913
TCGTATG579200.042.444843
CGTATGC581000.042.3838544
GTCACGC583350.042.28773529
ACGTCTG594150.042.257515
ACGCCAA584900.042.21421432
ATCTCGT582350.042.09529540
TCGGAAG604400.042.0684623
TCACGCC591350.041.7194630
CGGAAGA609700.041.676864
AGTCACG591750.041.65703628
CACGTCT604650.041.5497314
CACGCCA597200.041.3485331