FastQCFastQC Report
Fri 19 Sep 2014
SV53_ACAGTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV53_ACAGTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24045100
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA30650.026.50086241
CTCGTAT30450.026.45324742
TCGTATG31750.025.29925743
CGTATGC32450.024.96157544
GATCTCG36200.022.7482839
ATCTCGT36150.022.593440
CGTCTGA46200.018.60354216
ACACGTC47100.018.3913713
ATCGGAA48200.018.3001962
GATCGGA49100.017.8772981
ACGTCTG51100.016.8636715
GTATGCC52800.015.72462345
CACGTCT58200.014.88373814
TCGGAAG60100.014.7515453
CGGAAGA63150.014.1814914
CACACGT66550.013.59102612
GCACACG71050.012.6035611
AGTGATC71050.012.00190836
GTGATCT80000.010.51857237
GAACTCC96300.09.32225221
Produced by FastQC (version 0.11.2)