FastQCFastQC Report
Fri 19 Sep 2014
SV52_TGACCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV52_TGACCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32064893
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2339020.7294644644533821TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT263700.041.21203642
TCGTATG266700.040.7906443
TCTCGTA267000.040.67739541
CGTATGC267750.040.64780444
AATCTCG269350.040.4134439
ATCTCGT273500.039.7353440
ACACGTC283900.039.24558613
CGTCTGA283800.039.14842216
ATCGGAA291550.038.9741742
GATCGGA292900.038.8514751
ACGTCTG287500.038.70720715
GTATGCC288450.037.77009645
CACGTCT298900.037.2911414
CACACGT303000.036.88307612
TCGGAAG309050.036.7962653
CGGAAGA317900.035.722244
GCACACG315400.035.54715311
ACCAATC307250.035.41359336
GACCAAT311050.035.08222235
CCAATCT324500.033.6905837
Produced by FastQC (version 0.11.2)