FastQCFastQC Report
Fri 19 Sep 2014
SV51_CGATGT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV51_CGATGT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31897461
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA23300.017.57535641
CGATGTA22950.017.35269734
CTCGTAT23350.017.34502642
TCGTATG23650.017.02986543
TATCTCG24100.016.43127839
CGTATGC26100.016.03481144
CACCGAT27450.015.16372131
ACCGATG27800.014.73000932
CCGATGT28950.013.75628333
ATCTCGT32550.012.58082340
GATCGGA37900.012.5300591
ACACGTC37700.012.4135613
ATCGGAA39000.012.2314612
CGTCTGA37300.011.88315516
TCACCGA35650.011.54957330
GTCACCG35350.011.39299729
ACGTCTG42850.010.50155415
CGGAAGA51750.09.6091334
TCGGAAG51050.09.5646633
CACGTCT49250.09.45667614
Produced by FastQC (version 0.11.2)