##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	lane2_Undetermined_L002_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4530637
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.065521912260902	33.0	33.0	33.0	22.0	33.0
2	29.96261497003622	33.0	33.0	33.0	15.0	33.0
3	29.970221847391436	33.0	33.0	33.0	22.0	33.0
4	33.444175730697474	37.0	37.0	37.0	22.0	37.0
5	33.459209378283894	37.0	37.0	37.0	22.0	37.0
6	33.499627977257944	37.0	37.0	37.0	22.0	37.0
7	33.399173891000316	37.0	37.0	37.0	15.0	37.0
8	33.59518186956934	37.0	37.0	37.0	22.0	37.0
9	33.46395352353323	37.0	37.0	37.0	22.0	37.0
10-11	33.54772838786246	37.0	37.0	37.0	22.0	37.0
12-13	33.50308687277308	37.0	37.0	37.0	22.0	37.0
14-15	35.150820734479495	40.0	37.0	40.0	22.0	40.0
16-17	35.06673531779306	40.0	37.0	40.0	22.0	40.0
18-19	35.01992766138625	40.0	37.0	40.0	22.0	40.0
20-21	34.935280292815335	40.0	37.0	40.0	22.0	40.0
22-23	35.046309051023066	40.0	37.0	40.0	22.0	40.0
24-25	34.759869417920704	40.0	37.0	40.0	15.0	40.0
26-27	34.63834787470283	40.0	33.0	40.0	18.5	40.0
28-29	34.37906281169734	37.0	33.0	40.0	15.0	40.0
30-31	34.365494079530094	40.0	33.0	40.0	15.0	40.0
32-33	34.11582124103079	37.0	33.0	40.0	15.0	40.0
34-35	33.914822683874256	37.0	33.0	40.0	15.0	40.0
36-37	33.87139611935363	37.0	33.0	40.0	15.0	40.0
38-39	33.832446960548815	37.0	33.0	40.0	15.0	40.0
40-41	33.65936721480887	37.0	33.0	40.0	15.0	40.0
42-43	33.38719566365613	37.0	33.0	40.0	15.0	40.0
44-45	33.139208791169985	37.0	33.0	40.0	15.0	40.0
46-47	32.83090137214701	37.0	33.0	40.0	15.0	40.0
48-49	32.50101475796892	37.0	33.0	40.0	15.0	40.0
50-51	32.05068216676816	37.0	30.0	40.0	15.0	40.0
52-53	31.94233956505454	37.0	27.0	40.0	15.0	40.0
54-55	31.626223420680137	37.0	27.0	38.5	15.0	40.0
56-57	31.227665226766128	37.0	27.0	37.0	15.0	40.0
58-59	30.95059094339273	37.0	27.0	37.0	10.5	40.0
60-61	31.137316893849587	37.0	27.0	37.0	15.0	40.0
62-63	30.927351385688148	37.0	27.0	37.0	15.0	40.0
64-65	30.876233849677206	37.0	27.0	37.0	15.0	40.0
66-67	30.862610047991044	37.0	27.0	37.0	15.0	37.0
68-69	30.52102342341706	37.0	27.0	37.0	15.0	37.0
70-71	30.32559759256811	37.0	27.0	37.0	15.0	37.0
72-73	30.05870289321347	33.0	27.0	37.0	15.0	37.0
74-75	29.894628172594714	33.0	27.0	37.0	15.0	37.0
76-77	29.845237877146197	33.0	27.0	37.0	15.0	37.0
78-79	29.575910628019855	33.0	27.0	37.0	10.5	37.0
80-81	29.483651305544896	33.0	27.0	37.0	10.5	37.0
82-83	29.49189197898662	33.0	27.0	37.0	15.0	37.0
84-85	29.340195208753208	33.0	27.0	37.0	6.0	37.0
86-87	29.18593478135635	33.0	27.0	37.0	6.0	37.0
88-89	29.016156447757787	33.0	27.0	37.0	6.0	37.0
90-91	28.872123169435113	33.0	24.5	37.0	6.0	37.0
92-93	28.795511094797487	33.0	22.0	37.0	6.0	37.0
94-95	28.7306762823859	33.0	22.0	37.0	6.0	37.0
96-97	28.61996359452324	33.0	22.0	37.0	6.0	37.0
98-99	28.628074374530556	33.0	22.0	37.0	6.0	37.0
100-101	27.992998225194384	33.0	22.0	37.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	-0.28932186810490634
2201	2	-0.06145003081755718
2201	3	-0.07253568185888781
2201	4	-0.0013050015324651554
2201	5	0.07775994176500944
2201	6	-0.24850510770805556
2201	7	-0.3521227773451798
2201	8	-0.47227665181727474
2201	9	-0.24124032564815678
2201	10-11	0.1398212949956914
2201	12-13	0.2041369817558376
2201	14-15	0.5773760128128131
2201	16-17	0.8074847770126823
2201	18-19	0.8161930827438653
2201	20-21	0.6813229205054085
2201	22-23	0.6833122058464483
2201	24-25	0.9520539179279197
2201	26-27	0.8818208421756069
2201	28-29	0.9642174689952014
2201	30-31	0.8575718121233677
2201	32-33	0.9652938377289786
2201	34-35	0.9743267771015738
2201	36-37	0.9896784138441106
2201	38-39	0.9770797248513006
2201	40-41	0.9871834969235849
2201	42-43	0.9511075484512403
2201	44-45	0.8282882912183211
2201	46-47	0.939286169721143
2201	48-49	0.9022421979947595
2201	50-51	1.0015509851020923
2201	52-53	0.983905780743001
2201	54-55	0.9119868278466647
2201	56-57	0.9709121715891023
2201	58-59	0.9749165291869311
2201	60-61	0.8293866642359617
2201	62-63	0.8664660063925176
2201	64-65	0.906697067638035
2201	66-67	0.9051541377698982
2201	68-69	0.9035997230377717
2201	70-71	0.8395438540557478
2201	72-73	0.9040989086037214
2201	74-75	0.8701720703749309
2201	76-77	0.8461772447621634
2201	78-79	0.8971238588156218
2201	80-81	0.8312647383124165
2201	82-83	0.7978653596066536
2201	84-85	0.7695795960366851
2201	86-87	0.732950022842779
2201	88-89	0.7062120987309051
2201	90-91	0.6553715647790987
2201	92-93	0.786282069801505
2201	94-95	0.7784176504766975
2201	96-97	0.8112803730022762
2201	98-99	0.7093523375464699
2201	100-101	0.8502155028687994
1101	1	0.054531142802428434
1101	2	0.1603969506617453
1101	3	0.20965435307931912
1101	4	0.30338193155438375
1101	5	0.19175766310872433
1101	6	0.08627937458908264
1101	7	0.014743561708883135
1101	8	-0.04367090574915977
1101	9	0.2009913359568074
1101	10-11	0.2451812673734537
1101	12-13	0.4670104827259536
1101	14-15	0.8379144264017171
1101	16-17	0.639421013298211
1101	18-19	0.7927370418957906
1101	20-21	0.8539018035891033
1101	22-23	0.8767578422583213
1101	24-25	0.8831544907741389
1101	26-27	0.8352666778435136
1101	28-29	0.8548823759634558
1101	30-31	0.815860647421303
1101	32-33	0.8435443504415119
1101	34-35	1.0128869827575855
1101	36-37	0.9678834842855792
1101	38-39	0.8857708342850898
1101	40-41	0.8857097234782927
1101	42-43	0.8433855742581713
1101	44-45	0.8959737063905706
1101	46-47	0.8828741849764228
1101	48-49	1.0628934886385224
1101	50-51	1.045163483570498
1101	52-53	1.0077447574829748
1101	54-55	0.9990620853627767
1101	56-57	0.9428310859827711
1101	58-59	0.9545774325959364
1101	60-61	1.0042384043656511
1101	62-63	0.8782680972723007
1101	64-65	0.8854597947453016
1101	66-67	0.8513502137346265
1101	68-69	0.8941428310393
1101	70-71	0.8666903322202586
1101	72-73	0.843938940211082
1101	74-75	0.8146203809811823
1101	76-77	0.8699904594816523
1101	78-79	0.9615615493727887
1101	80-81	0.8349482239725283
1101	82-83	0.7412676503967432
1101	84-85	0.5258046741628952
1101	86-87	0.7075118677513998
1101	88-89	0.8656374443360271
1101	90-91	0.7986977293562525
1101	92-93	0.8058766328478981
1101	94-95	0.7663928463721419
1101	96-97	0.8288734909963438
1101	98-99	0.8504288420484656
1101	100-101	0.8799988149085038
2202	1	-0.05236926044412016
2202	2	0.07477230434339788
2202	3	0.140056283834884
2202	4	0.21904735761464167
2202	5	0.14814268046138324
2202	6	0.018424832228411958
2202	7	-0.0045479669477401785
2202	8	-0.04385120919317842
2202	9	0.22697095993633098
2202	10-11	0.2916061147214819
2202	12-13	0.5502768886080531
2202	14-15	0.6379273864846553
2202	16-17	0.7292398788259788
2202	18-19	0.7028820414802794
2202	20-21	0.7437980091538279
2202	22-23	0.6493826844618908
2202	24-25	0.715809225260621
2202	26-27	0.6753769968396313
2202	28-29	0.7306032187447826
2202	30-31	0.6716387915770028
2202	32-33	0.7762180303927622
2202	34-35	0.8104322836887405
2202	36-37	0.7813756478873302
2202	38-39	0.7563610176653199
2202	40-41	0.7858125879534228
2202	42-43	0.7025532005645658
2202	44-45	0.6270732061907225
2202	46-47	0.7110028578243544
2202	48-49	0.8125044388203619
2202	50-51	0.7125175540258653
2202	52-53	0.7902059563796868
2202	54-55	0.8855695013003135
2202	56-57	0.7783819538136605
2202	58-59	0.8307082987647796
2202	60-61	0.7194567322973029
2202	62-63	0.6920868846438388
2202	64-65	0.8048260170097343
2202	66-67	0.769718435413381
2202	68-69	0.634019200751883
2202	70-71	0.6951280684202459
2202	72-73	0.6660001424714785
2202	74-75	0.5254827112634572
2202	76-77	0.5986968206069427
2202	78-79	0.5643596906396731
2202	80-81	0.5764971121754883
2202	82-83	0.4422979769908366
2202	84-85	0.4841625673480685
2202	86-87	0.5969154664993184
2202	88-89	0.45634041506405154
2202	90-91	0.3593246263760257
2202	92-93	0.5066887898014301
2202	94-95	0.4229828012196073
2202	96-97	0.4651043427211228
2202	98-99	0.632089557959489
2202	100-101	0.6065501417835577
1102	1	-0.034997346817114305
1102	2	-0.034373462354441386
1102	3	0.016146404740066345
1102	4	0.15240866640567674
1102	5	0.22324628478553166
1102	6	-0.052632524976374384
1102	7	-0.01911672092179373
1102	8	-0.1306222236123986
1102	9	0.2166397009209362
1102	10-11	0.30891245866740036
1102	12-13	0.4494013342958496
1102	14-15	0.5603317349653736
1102	16-17	0.6362708324602409
1102	18-19	0.7007462057175857
1102	20-21	0.6429058011056767
1102	22-23	0.7028650064859434
1102	24-25	0.6402036482086757
1102	26-27	0.6773720509854115
1102	28-29	0.7730601451134262
1102	30-31	0.6359743110361507
1102	32-33	0.6014396906786317
1102	34-35	0.5268614290890028
1102	36-37	0.6075562133768031
1102	38-39	0.6200962040027704
1102	40-41	0.7094047659120903
1102	42-43	0.6678920312749952
1102	44-45	0.5767535319744397
1102	46-47	0.6807987708707159
1102	48-49	0.6141633176927428
1102	50-51	0.5582943051755933
1102	52-53	0.5571060454736525
1102	54-55	0.4940144604681258
1102	56-57	0.47552758803326256
1102	58-59	0.5994117831068593
1102	60-61	0.5173516437001311
1102	62-63	0.4374280783526281
1102	64-65	0.482803459536143
1102	66-67	0.48636753227118135
1102	68-69	0.3804953500886832
1102	70-71	0.4961600480561792
1102	72-73	0.4277426353126046
1102	74-75	0.4159654535066082
1102	76-77	0.48546573275625704
1102	78-79	0.4198439373564291
1102	80-81	0.4181991944562178
1102	82-83	0.2445809716473093
1102	84-85	0.23317764802238017
1102	86-87	0.3838597361460856
1102	88-89	0.39384718871863456
1102	90-91	0.35647771836877595
1102	92-93	0.373201344815719
1102	94-95	0.3001898616520009
1102	96-97	0.35098478158112556
1102	98-99	0.4092973465524068
1102	100-101	0.33259409496236714
2203	1	-0.34964662135499935
2203	2	-0.3348373039026207
2203	3	-0.31951574963051144
2203	4	-0.2527386364142643
2203	5	-0.10381313795366509
2203	6	-0.4043089317202373
2203	7	-0.4340844689740564
2203	8	-0.36747223744804813
2203	9	-0.16376498357663394
2203	10-11	-0.2264136602839102
2203	12-13	-0.028139635213904057
2203	14-15	0.04705067944307473
2203	16-17	0.18383331061810537
2203	18-19	0.10947249907525958
2203	20-21	0.042182180067996455
2203	22-23	0.019059155384006488
2203	24-25	-0.01442121825801479
2203	26-27	0.05392202414094527
2203	28-29	0.07229204053134453
2203	30-31	-0.01647486362046635
2203	32-33	0.04200386549509716
2203	34-35	-0.04042796310151431
2203	36-37	0.07523733636856633
2203	38-39	-0.0773370899733763
2203	40-41	0.015612794557952725
2203	42-43	-0.05814040037083146
2203	44-45	-0.07834312464897408
2203	46-47	0.13085142351211232
2203	48-49	-0.05064911829671104
2203	50-51	0.01608227686929098
2203	52-53	-0.020351535305046298
2203	54-55	0.033240546015438355
2203	56-57	-0.026030074656894442
2203	58-59	0.11893547293693985
2203	60-61	-0.019027902909471095
2203	62-63	0.014134653428389043
2203	64-65	-0.0847499896197732
2203	66-67	-0.11782989529009313
2203	68-69	-0.12286175189935378
2203	70-71	-0.020169635090898197
2203	72-73	-0.1432475672832041
2203	74-75	-0.05982418728464012
2203	76-77	-0.05351418846322176
2203	78-79	0.009833916156470224
2203	80-81	-0.1770898603869746
2203	82-83	-0.1684586269755215
2203	84-85	-0.2340446274845469
2203	86-87	-0.11348514196238568
2203	88-89	-0.14275188721427767
2203	90-91	-0.19477919807822985
2203	92-93	-0.06130147027054278
2203	94-95	-0.20844538100105225
2203	96-97	-0.27245170727263357
2203	98-99	-0.2783877893291091
2203	100-101	-0.24328434755133088
1103	1	-0.022720754365352036
1103	2	0.011462402107948577
1103	3	0.06416940465488352
1103	4	0.22217498137370484
1103	5	0.2373628197668296
1103	6	0.03897735041942241
1103	7	0.016064673216753533
1103	8	0.03402047294552091
1103	9	0.20761401797599177
1103	10-11	0.3038232062912769
1103	12-13	0.48498234355614755
1103	14-15	0.537200981270793
1103	16-17	0.5388684540587505
1103	18-19	0.5160053780027241
1103	20-21	0.47097085342190326
1103	22-23	0.5501898013661801
1103	24-25	0.5934718073849936
1103	26-27	0.5775591179190585
1103	28-29	0.5592997478218109
1103	30-31	0.4781186729070228
1103	32-33	0.48956471958915415
1103	34-35	0.5254883540634765
1103	36-37	0.5239717000607627
1103	38-39	0.4598143478690204
1103	40-41	0.5021144277132592
1103	42-43	0.561282508470974
1103	44-45	0.606212208284937
1103	46-47	0.5444889897348943
1103	48-49	0.5205490013735243
1103	50-51	0.528908913299496
1103	52-53	0.5254322396566735
1103	54-55	0.4228049520343866
1103	56-57	0.48934741936775694
1103	58-59	0.4158646685140006
1103	60-61	0.5379418370097397
1103	62-63	0.4853335032403798
1103	64-65	0.5233065233520549
1103	66-67	0.4839958026932081
1103	68-69	0.5214799716217335
1103	70-71	0.303626908761629
1103	72-73	0.44176835624947586
1103	74-75	0.3383885974974916
1103	76-77	0.27487836151390965
1103	78-79	0.15201622396275738
1103	80-81	0.24909267934669543
1103	82-83	0.19237459361533737
1103	84-85	0.17618448670317832
1103	86-87	0.2713771941046801
1103	88-89	0.3432077445365813
1103	90-91	0.37437635003274394
1103	92-93	0.3961955608442338
1103	94-95	0.4082753376302364
1103	96-97	0.2685593887345661
1103	98-99	0.24453604185352518
1103	100-101	0.35949751400949737
2204	1	0.03083243050644313
2204	2	0.056653382501316685
2204	3	-0.00257761030558612
2204	4	0.31722597447587475
2204	5	0.34435723229052684
2204	6	0.10681338428620535
2204	7	0.1255693906108135
2204	8	0.04710543547310664
2204	9	0.21385538624169698
2204	10-11	0.31942967370240893
2204	12-13	0.3331294966243661
2204	14-15	0.3767611786381977
2204	16-17	0.3170232917950173
2204	18-19	0.32569057009196456
2204	20-21	0.289524675106712
2204	22-23	0.14556048833122048
2204	24-25	-0.010678681484854735
2204	26-27	0.01038881970176675
2204	28-29	-0.0850184297051797
2204	30-31	0.09643443208940283
2204	32-33	-0.0036162317717227666
2204	34-35	-0.10797574478775829
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1213	82-83	-2.701520101693763
1213	84-85	-2.5689454428629226
1213	86-87	-2.8668056276708285
1213	88-89	-2.755151347219087
1213	90-91	-2.819323002674654
1213	92-93	-2.9258373400972317
1213	94-95	-2.9164064862677996
1213	96-97	-2.927759745086661
1213	98-99	-2.9762292088270996
1213	100-101	-3.129802527509039
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54043.0
3	5140.0
4	1279.0
5	374.0
6	358.0
7	1178.0
8	6731.0
9	21052.0
10	59005.0
11	81192.0
12	48719.0
13	35318.0
14	31094.0
15	29630.0
16	29831.0
17	31519.0
18	33528.0
19	35774.0
20	39498.0
21	45108.0
22	51047.0
23	57700.0
24	64273.0
25	71172.0
26	80994.0
27	95079.0
28	111046.0
29	121895.0
30	128199.0
31	141883.0
32	163029.0
33	189978.0
34	226943.0
35	290071.0
36	483914.0
37	1175086.0
38	487916.0
39	41.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.66960923821333	18.293550043794376	18.396401161932317	30.640439556059974
2	22.524512839811845	24.836782244037988	34.82812076613581	17.81058415001436
3	22.468850589330177	25.64614215554326	26.983498575263003	24.90150867986355
4	23.403233109862487	34.39961151650516	21.960480519223825	20.23667485440853
5	24.10378757384109	31.86446263848045	24.28666584798743	19.74508393969103
6	21.584270151984622	32.338708458848274	25.322283131941848	20.754738257225252
7	19.413903309568372	20.540436023924073	36.101804713393854	23.943855953113697
8	21.112650320635414	23.473899929445626	30.568753498274276	24.84469625164468
9	24.467933945065806	25.032339833074772	29.00698978976479	21.49273643209463
10-11	23.569335334453818	31.592779141093313	24.364727029624817	20.473158494828052
12-13	23.632324167950358	24.494829999093863	28.40728397920589	23.465561853749886
14-15	24.690040675990087	23.9151773301983	29.130419945612434	22.264362048199175
16-17	24.866203491550326	25.54921613290742	27.24871854697971	22.33586182856254
18-19	23.940155654931992	24.69753350485059	29.10161177420521	22.260699066012208
20-21	25.394807897769965	26.07234428215891	27.84641627985911	20.686431540212016
22-23	26.70749444911606	25.158979779442635	26.685548443009242	21.447977328432057
24-25	24.31520543976783	26.06048518900208	29.004655833700166	20.619653537529924
26-27	24.604902251765402	27.616847080432994	27.410116984280762	20.36813368352084
28-29	24.27706077967275	25.645553167159363	27.99263322864697	22.08475282452092
30-31	24.509468647340256	23.587994674439123	29.95011712693117	21.95241955128945
32-33	23.17880765017329	25.60465110024581	30.075660389537042	21.140880860043858
34-35	22.699299339048995	26.10570465211442	30.573230565544048	20.621765443292535
36-37	21.759975016113398	25.953972476055053	30.65712416019349	21.62892834763806
38-39	22.52571522517383	25.016367746702766	30.032524946954613	22.425392081168795
40-41	24.734976278481778	25.335790771394436	28.998388705937156	20.930844244186634
42-43	25.15335809333092	24.401826191623393	30.118526472658623	20.326289242387062
44-45	25.77172002060562	25.25607584765902	29.30297310590737	19.66923102582799
46-47	24.450388905261715	24.739133894869603	28.712234507001817	22.09824269286687
48-49	23.189268902636528	24.998360326573703	28.57763870461994	23.234732066169833
50-51	24.713650629371006	24.89013593733283	28.591011582002213	21.805201851293948
52-53	23.251869023698454	25.916281838869757	28.891779876821534	21.94006926061025
54-55	22.842863852176663	26.08060049256988	28.213283272889512	22.863252382363942
56-57	22.73625111255784	24.9656861088551	30.462712163689737	21.83535061489732
58-59	23.13782914414968	26.5475316860376	29.08723756477026	21.227401605042463
60-61	23.290248580342528	27.82069891982839	28.419858096352645	20.46919440347644
62-63	23.631862645984015	28.488860328545645	26.897111040219997	20.982165985250344
64-65	21.887809667075373	29.685978602897716	28.1678571724289	20.258354557598004
66-67	23.887998489030476	28.37766137185828	27.17387607096007	20.56046406815117
68-69	23.148284275059304	27.198928856495908	28.42141937926576	21.23136748917903
70-71	24.1134311371611	26.86952452015829	28.18421589388843	20.832828448792178
72-73	24.876259901473315	26.936717272487765	26.79997471666725	21.38704810937167
74-75	24.0970446353728	27.153003329157947	27.888452009235593	20.861500026233664
76-77	23.665804141975492	27.197147504698655	28.264338629334123	20.872709723991726
78-79	25.11221662282552	25.542604061006823	27.625871211290747	21.7193081048769
80-81	25.408335850973312	25.269737952547416	27.801836883550585	21.520089312928686
82-83	23.989877099033848	26.829602494372285	27.579424225052783	21.601096181541084
84-85	24.52655538321558	27.037715161167707	26.781263430269536	21.654466025347176
86-87	24.251896644959295	27.10505832397195	27.41872152851159	21.224323502557162
88-89	24.620805936389527	26.808981420478183	27.46946158845273	21.100751054679552
90-91	25.351421208578596	26.767667783145544	26.54533420020159	21.335576808074272
92-93	24.5741669388458	27.191472787289968	27.039388152504007	21.194972121360227
94-95	24.13606064895818	26.756624737089187	26.922213089391633	22.185101524561006
96-97	24.753285240089443	27.374818419279677	26.874606901522256	20.997289439108627
98-99	26.280190651938874	26.58343339377443	26.099695682683468	21.036680271603235
100-101	25.18935817415568	25.48004039193294	26.191902874886086	23.138698559025293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	42461.0
1	25572.5
2	7773.5
3	5259.5
4	2661.0
5	1321.5
6	854.5
7	664.5
8	609.0
9	666.0
10	702.0
11	755.0
12	849.5
13	958.0
14	1100.5
15	1249.5
16	1517.5
17	1900.5
18	2289.0
19	2780.0
20	3648.5
21	6640.0
22	10997.5
23	13265.0
24	11751.0
25	9693.0
26	8412.0
27	7063.0
28	6923.0
29	6538.5
30	6493.5
31	7396.5
32	8366.0
33	9978.5
34	15265.5
35	21641.0
36	33630.0
37	53482.5
38	76541.0
39	94245.5
40	114347.5
41	157048.0
42	207436.5
43	248398.5
44	301491.0
45	369696.5
46	420976.5
47	433066.5
48	395517.0
49	323945.0
50	247092.0
51	191803.5
52	155862.5
53	134247.5
54	111375.0
55	72995.5
56	43689.0
57	26603.0
58	15641.5
59	10565.0
60	7972.5
61	7288.5
62	8237.0
63	7654.5
64	5436.5
65	3526.0
66	2389.0
67	1804.0
68	1118.0
69	805.5
70	554.5
71	454.0
72	428.5
73	440.0
74	452.5
75	423.5
76	342.0
77	344.5
78	309.0
79	181.0
80	145.5
81	117.5
82	116.0
83	119.0
84	106.5
85	97.0
86	89.0
87	79.5
88	69.5
89	76.0
90	81.0
91	83.0
92	77.5
93	76.5
94	81.5
95	83.0
96	95.5
97	133.5
98	169.5
99	247.5
100	2004.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.528231681328696
2	2.171173722370607
3	1.7655574701747236
4	1.7316108088112112
5	1.6842664729043622
6	2.131488353624446
7	1.6009448560986017
8	1.6130623574565783
9	1.3616186862906914
10-11	1.3495011849327148
12-13	1.3486403788253176
14-15	1.3637044857047695
16-17	1.3809206078527148
18-19	1.4004211769779835
20-21	1.4161584783773231
22-23	1.4375903432563677
24-25	1.4477765488605685
26-27	1.464738843566589
28-29	1.4925826103481694
30-31	1.5093683294424163
32-33	1.5168948648942744
34-35	1.5070507745378852
36-37	1.4779378705466801
38-39	1.4581614020280151
40-41	1.4409011359771264
42-43	1.4190719759715908
44-45	1.4102652673343727
46-47	1.4025952641979482
48-49	1.3968013769366205
50-51	1.38706764633759
52-53	1.3845624798455494
54-55	1.3781395419672775
56-57	1.3757447352325953
58-59	1.3760537425531996
60-61	1.369376977233003
62-63	1.3712310211566276
64-65	1.370403322976438
66-67	1.368085768071907
68-69	1.363340298505486
70-71	1.363903133268015
72-73	1.3527788697262657
74-75	1.3505496026276216
76-77	1.3465325074597678
78-79	1.340010245799873
80-81	1.3356068914812642
82-83	1.3369974244239828
84-85	1.3417539299661394
86-87	1.3471394861252401
88-89	1.3549639929219666
90-91	1.3613207149458233
92-93	1.370061207728626
94-95	1.3823552846983769
96-97	1.388921690261215
98-99	1.4071641581525953
100-101	1.4104749508733538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4530637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.037992819561143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.48577911768218	13.15610518185417
2	4.911259102895006	1.477109582486788
3	1.7829833909314707	0.804374742906727
4	0.97902359058612	0.5889019890166016
5	0.4036460126538913	0.3035012919966852
6	0.39454212949405243	0.3559873026207554
7	0.1816951479248375	0.19126312308899643
8	0.20983811238700473	0.2524435221876833
9	0.06440080281821593	0.08716129293188739
>10	1.1039986013276988	3.411507376960983
>50	0.5899700402193052	6.896399416664152
>100	1.791811284448264	47.050062544588926
>500	0.058436705885812394	6.134655168633128
>1k	0.03862999608531406	10.945443538883378
>5k	0.0020667965999847525	2.257818665591123
>10k+	0.001919168060746479	6.087265259588073
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCAAATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTAT	57476	1.268607482788844	No Hit
CTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATG	32611	0.7197884094444115	No Hit
GGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTT	27868	0.6151011436140217	No Hit
CTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGT	27699	0.6113709838153001	No Hit
TGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGG	18639	0.41139910348147507	No Hit
GAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGC	17051	0.37634884454437645	No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGGAAATC	15191	0.33529501480696866	No Hit
TGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTT	14437	0.31865276339728826	No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGG	14397	0.31776988533841927	No Hit
AAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCT	14127	0.31181045844105365	No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGGAAATCTGGCCG	12497	0.27583317754214254	No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTGGGAAAT	11568	0.25532833462491034	No Hit
CTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTT	10636	0.234757275853263	No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTG	9715	0.21442900854780467	No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGGAAATCT	9155	0.20206871572363885	No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTGGGAAATCTGGCC	8594	0.18968635094800135	No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGG	8167	0.18026162766957493	No Hit
ATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCA	7879	0.17390490564571826	No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGGAAATCTGGCCGC	7631	0.16843106168073055	No Hit
GCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTT	6994	0.15437122859324198	No Hit
CGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTT	6800	0.1500892700077274	No Hit
AAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTT	6474	0.14289381382794517	No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTT	6375	0.14070869063224442	No Hit
GCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTT	6307	0.13920779793216714	No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA	6232	0.1375524015717878	No Hit
CGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTG	6016	0.13278486005389528	No Hit
GAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTT	5363	0.11837187574285912	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	4840	0.10682824512314715	No Hit
GGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGG	4825	0.10649716585107129	No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTTGGGAAA	4789	0.10570257559808918	No Hit
CGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTT	4663	0.10292150971265189	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	4575	0.10097917798314011	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0017657561177379694	0.0	0.0
2	0.001831971972153143	0.0	0.0
3	0.001964403680983491	0.0	0.0
4	0.0021189073412855633	0.0	0.0
5	0.00229548295305936	0.0	0.0
6	0.0023396268560028095	0.0	0.0
7	0.0023396268560028095	0.0	0.0
8	0.0024941305163048814	0.0	0.0
9	0.0028472817398524754	0.0	0.0
10-11	0.003498404308268352	0.0	0.0
12-13	0.003763267725929047	0.0	0.0
14-15	0.004436462245816648	0.0	0.0
16-17	0.004933081153930452	0.0	0.0
18-19	0.005396592134836668	0.0	0.0
20-21	0.0057607793341201245	0.0	0.0
22-23	0.006268434217969791	0.0	0.0
24-25	0.006809197029027044	0.0	0.0
26-27	0.007703111063631891	0.0	0.0
28-29	0.009722694623294694	0.0	0.0
30-31	0.012592048314618893	0.0	0.0
32-33	0.01482131541326308	0.0	0.0
34-35	0.0186507989936073	0.0	0.0
36-37	0.023429376487235677	0.0	0.0
38-39	0.02879286069486476	0.0	0.0
40-41	0.035414446136382144	0.0	0.0
42-43	0.04270922609778713	0.0	0.0
44-45	0.05110760363277835	0.0	0.0
46-47	0.061404168994337885	0.0	0.0
48-49	0.07396310938174919	0.0	0.0
50-51	0.08952383516931503	0.0	0.0
52-53	0.10694964085624163	1.1035975735862308E-5	0.0
54-55	0.12792903073011586	2.2071951471724616E-5	0.0
56-57	0.14893049255546187	2.2071951471724616E-5	0.0
58-59	0.1710245159786582	2.2071951471724616E-5	0.0
60-61	0.19634104431672633	2.2071951471724616E-5	0.0
62-63	0.2267672294204987	2.2071951471724616E-5	0.0
64-65	0.2625458627561643	2.2071951471724616E-5	0.0
66-67	0.30127110161330517	2.2071951471724616E-5	0.0
68-69	0.3448521697942254	2.2071951471724616E-5	0.0
70-71	0.3935428947408499	2.2071951471724616E-5	0.0
72-73	0.4537441423799788	2.2071951471724616E-5	0.0
74-75	0.5254338407601404	2.2071951471724616E-5	0.0
76-77	0.5972007909704529	2.2071951471724616E-5	0.0
78-79	0.6790656589790796	2.2071951471724616E-5	2.2071951471724616E-5
80-81	0.7778928216937265	2.2071951471724616E-5	2.2071951471724616E-5
82-83	0.8870055137941971	2.2071951471724616E-5	3.310792720758692E-5
84-85	1.003556453540639	2.2071951471724616E-5	4.414390294344923E-5
86-87	1.1325780458686054	3.310792720758692E-5	4.414390294344923E-5
88-89	1.2785950408297995	4.414390294344923E-5	4.414390294344923E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAATC	540	0.0	68.554	2
GTCCAAT	605	0.0	62.802227	1
GAGCGGC	650	0.0	62.796135	9
ACGGGAG	920	0.0	61.604385	3
ATCCGGA	585	0.0	55.884182	2
GATTGGG	645	0.0	55.179195	1
GATCCGG	640	0.0	51.10134	1
CCAGACG	335	0.0	45.710064	6
TCCGGAA	535	0.0	44.888355	3
CAGACGT	340	0.0	44.799698	7
GGGAGAG	4140	0.0	44.79256	5
GAACCGG	595	0.0	43.649586	1
GCATTCC	235	0.0	42.920906	3
CAACTAG	13565	0.0	42.693108	8
TCGGGGG	4160	0.0	42.4645	38-39
AACTAGC	13715	0.0	42.01174	9
TCATTTA	1465	0.0	39.517754	52-53
GCCAAAT	15445	0.0	38.955853	1
TCAAAGG	930	0.0	38.89866	7
CCAAATC	15210	0.0	38.594223	2
>>END_MODULE