FastQCFastQC Report
Thu 14 Aug 2014
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4530637
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTAT574761.268607482788844No Hit
CTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATG326110.7197884094444115No Hit
GGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTT278680.6151011436140217No Hit
CTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGT276990.6113709838153001No Hit
TGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGG186390.41139910348147507No Hit
GAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGC170510.37634884454437645No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGGAAATC151910.33529501480696866No Hit
TGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTT144370.31865276339728826No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGG143970.31776988533841927No Hit
AAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCT141270.31181045844105365No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGGAAATCTGGCCG124970.27583317754214254No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTGGGAAAT115680.25532833462491034No Hit
CTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTT106360.234757275853263No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTG97150.21442900854780467No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGGAAATCT91550.20206871572363885No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTGGGAAATCTGGCC85940.18968635094800135No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGG81670.18026162766957493No Hit
ATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCA78790.17390490564571826No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGGAAATCTGGCCGC76310.16843106168073055No Hit
GCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTT69940.15437122859324198No Hit
CGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTT68000.1500892700077274No Hit
AAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTT64740.14289381382794517No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTT63750.14070869063224442No Hit
GCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTT63070.13920779793216714No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA62320.1375524015717878No Hit
CGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTG60160.13278486005389528No Hit
GAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTT53630.11837187574285912No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA48400.10682824512314715No Hit
GGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGG48250.10649716585107129No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTTGGGAAA47890.10570257559808918No Hit
CGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTT46630.10292150971265189No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG45750.10097917798314011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAATC5400.068.5542
GTCCAAT6050.062.8022271
GAGCGGC6500.062.7961359
ACGGGAG9200.061.6043853
ATCCGGA5850.055.8841822
GATTGGG6450.055.1791951
GATCCGG6400.051.101341
CCAGACG3350.045.7100646
TCCGGAA5350.044.8883553
CAGACGT3400.044.7996987
GGGAGAG41400.044.792565
GAACCGG5950.043.6495861
GCATTCC2350.042.9209063
CAACTAG135650.042.6931088
TCGGGGG41600.042.464538-39
AACTAGC137150.042.011749
TCATTTA14650.039.51775452-53
GCCAAAT154450.038.9558531
TCAAAGG9300.038.898667
CCAAATC152100.038.5942232
Produced by FastQC (version 0.11.2)