##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	lane2_Undetermined_L002_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4530637
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.556320446771615	33.0	33.0	33.0	33.0	33.0
2	32.52845129724584	33.0	33.0	33.0	33.0	33.0
3	32.519336464166074	33.0	33.0	33.0	33.0	33.0
4	36.33230978337042	37.0	37.0	37.0	37.0	37.0
5	36.1706876979992	37.0	37.0	37.0	33.0	37.0
6	36.0975981964567	37.0	37.0	37.0	33.0	37.0
7	35.948431092581465	37.0	37.0	37.0	33.0	37.0
8	35.93559713567871	37.0	37.0	37.0	33.0	37.0
9	35.9241629819383	37.0	37.0	37.0	33.0	37.0
10-11	35.89461007359451	37.0	37.0	37.0	33.0	37.0
12-13	35.955659215249426	37.0	37.0	37.0	33.0	37.0
14-15	37.692441151211185	40.0	37.0	40.0	33.0	40.0
16-17	37.73127608766714	40.0	37.0	40.0	33.0	40.0
18-19	37.816318102730364	40.0	37.0	40.0	33.0	40.0
20-21	37.92032599389446	40.0	37.0	40.0	33.0	40.0
22-23	37.8242453544612	40.0	37.0	40.0	33.0	40.0
24-25	37.84904848920803	40.0	37.0	40.0	33.0	40.0
26-27	37.79136267151837	40.0	37.0	40.0	33.0	40.0
28-29	37.63740595417377	40.0	37.0	40.0	33.0	40.0
30-31	37.488231566554546	40.0	37.0	40.0	33.0	40.0
32-33	37.20244272494133	40.0	37.0	40.0	33.0	40.0
34-35	36.839345328261786	40.0	37.0	40.0	33.0	40.0
36-37	36.45420754300113	40.0	37.0	40.0	33.0	40.0
38-39	36.333731327404955	40.0	37.0	40.0	33.0	40.0
40-41	36.185235321214215	40.0	37.0	40.0	33.0	40.0
42-43	35.97856217569406	38.5	37.0	40.0	27.0	40.0
44-45	36.0366175882111	37.0	37.0	40.0	27.0	40.0
46-47	35.72217482883754	37.0	37.0	40.0	27.0	40.0
48-49	35.588005284908064	37.0	37.0	40.0	27.0	40.0
50-51	35.07901670339072	37.0	37.0	40.0	27.0	40.0
52-53	34.78189534937361	37.0	33.0	40.0	27.0	40.0
54-55	34.80915089864847	37.0	33.0	40.0	27.0	40.0
56-57	34.735842443347366	37.0	33.0	40.0	27.0	40.0
58-59	33.945843045911644	37.0	33.0	38.5	22.0	40.0
60-61	34.11266539340936	37.0	33.0	37.0	27.0	40.0
62-63	34.275623383643406	37.0	33.0	37.0	27.0	40.0
64-65	33.987775118598115	37.0	33.0	37.0	27.0	40.0
66-67	33.96579873867626	37.0	33.0	37.0	27.0	40.0
68-69	33.50317129798746	37.0	33.0	37.0	27.0	37.0
70-71	33.282263178444886	37.0	33.0	37.0	27.0	37.0
72-73	32.940177286328606	37.0	33.0	37.0	22.0	37.0
74-75	32.428395940791546	37.0	33.0	37.0	22.0	37.0
76-77	32.11860517626992	37.0	33.0	37.0	18.5	37.0
78-79	32.04914022023834	37.0	33.0	37.0	18.5	37.0
80-81	31.805123319303668	37.0	33.0	37.0	15.0	37.0
82-83	31.921586854122282	37.0	33.0	37.0	15.0	37.0
84-85	31.92464823379141	37.0	33.0	37.0	15.0	37.0
86-87	31.7375225602934	37.0	33.0	37.0	15.0	37.0
88-89	31.74728332903298	37.0	33.0	37.0	15.0	37.0
90-91	31.784970634372165	37.0	33.0	37.0	15.0	37.0
92-93	31.552336569890723	37.0	33.0	37.0	15.0	37.0
94-95	31.425638160814913	37.0	33.0	37.0	15.0	37.0
96-97	31.51516729325258	37.0	33.0	37.0	15.0	37.0
98-99	31.30299315526713	37.0	33.0	37.0	15.0	37.0
100-101	30.80791354504896	35.0	30.0	37.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.12273240123643347
2201	2	0.14323705929739106
2201	3	0.1662114331672484
2201	4	0.17773633149731438
2201	5	0.1911321963862136
2201	6	0.2451638725233778
2201	7	0.36207630364361876
2201	8	0.2984288606941021
2201	9	0.21036578049348265
2201	10-11	0.26855159110675686
2201	12-13	0.2960540494527706
2201	14-15	0.4182757951644902
2201	16-17	0.400688584937285
2201	18-19	0.45893824190869736
2201	20-21	0.4469559458116734
2201	22-23	0.3427020313988791
2201	24-25	-0.02166386674819165
2201	26-27	0.11861451825221536
2201	28-29	0.03810076973218912
2201	30-31	-0.1370133205357007
2201	32-33	-0.08228162730780753
2201	34-35	0.029101775989850864
2201	36-37	0.3977580488982966
2201	38-39	0.580024790482895
2201	40-41	0.41629967565118875
2201	42-43	0.3869213815735435
2201	44-45	0.1896063299276065
2201	46-47	0.12066362861484237
2201	48-49	-0.2657222893024027
2201	50-51	-0.0419376888128582
2201	52-53	0.18165053358680439
2201	54-55	0.036987396395929295
2201	56-57	0.18946778507060458
2201	58-59	0.13118508950724106
2201	60-61	-0.08274948965622997
2201	62-63	-0.07037751190031116
2201	64-65	-0.342023996163455
2201	66-67	-0.030794803439164298
2201	68-69	0.2430276927709798
2201	70-71	0.18892739122119195
2201	72-73	-0.10999939609463638
2201	74-75	-0.041478385977619325
2201	76-77	-0.059210616818816675
2201	78-79	0.29916065106527867
2201	80-81	-0.057027784532085235
2201	82-83	0.15779264062011578
2201	84-85	0.21973300832128118
2201	86-87	0.33292024998576863
2201	88-89	0.19534937539245334
2201	90-91	0.13222789488697728
2201	92-93	0.19853111382960265
2201	94-95	0.31450231941147777
2201	96-97	0.18596255068159806
2201	98-99	0.33960157517488554
2201	100-101	0.2927913548735006
1101	1	0.09645201766559808
1101	2	0.1091320846772561
1101	3	0.14900396481327505
1101	4	0.22787375867964244
1101	5	0.17767921724087898
1101	6	0.24971352712643125
1101	7	0.2694830666907251
1101	8	0.26998171647912983
1101	9	0.28540976551894204
1101	10-11	0.2737929169266522
1101	12-13	0.21810810107138678
1101	14-15	0.14825632606583383
1101	16-17	0.272398815812565
1101	18-19	0.2832889200334705
1101	20-21	0.3207345732528637
1101	22-23	0.3732724047630427
1101	24-25	0.3652268856306833
1101	26-27	0.16393916878092796
1101	28-29	0.06602631667512782
1101	30-31	0.3022668982771606
1101	32-33	0.18645665524795874
1101	34-35	0.37327950731420145
1101	36-37	0.4562228168631606
1101	38-39	0.5055304695771881
1101	40-41	0.36196103169852023
1101	42-43	0.29719531252032994
1101	44-45	0.04321258900565539
1101	46-47	0.01480443809395382
1101	48-49	-0.05197859709409158
1101	50-51	0.10712080413347991
1101	52-53	0.20126911144595283
1101	54-55	0.07958677459815533
1101	56-57	0.2615332641713266
1101	58-59	0.2836073662720935
1101	60-61	-4.001688516410695E-4
1101	62-63	0.0869730802767279
1101	64-65	-0.009309992211647966
1101	66-67	0.0811396514379652
1101	68-69	0.29386021123636397
1101	70-71	0.1867832699283909
1101	72-73	-0.11290864567857284
1101	74-75	0.07993961510966585
1101	76-77	0.1900352214067027
1101	78-79	0.32430401131900055
1101	80-81	0.4323040995853624
1101	82-83	0.44184597967537087
1101	84-85	0.23335754839672163
1101	86-87	0.03891269669519204
1101	88-89	0.2763922680785029
1101	90-91	0.19924346035180207
1101	92-93	0.43804162205738706
1101	94-95	0.446536837991065
1101	96-97	0.013005898979951525
1101	98-99	0.13506949673819335
1101	100-101	0.08695734644883757
2202	1	0.08885325391900523
2202	2	0.10842793665926109
2202	3	0.10352876506970432
2202	4	0.15148772232809904
2202	5	0.14402826294347904
2202	6	0.1517742297805782
2202	7	0.27277463096284293
2202	8	0.2758414052788467
2202	9	0.21006993433638854
2202	10-11	0.21134886293356203
2202	12-13	0.1707874498222708
2202	14-15	0.21901840367595327
2202	16-17	0.2110233998037927
2202	18-19	0.10086956934352287
2202	20-21	0.0936711252376341
2202	22-23	0.17357422135115286
2202	24-25	0.256561388339442
2202	26-27	0.3152641641311291
2202	28-29	0.06384281476178444
2202	30-31	0.2385222356548269
2202	32-33	0.12372191450896963
2202	34-35	0.275943175354044
2202	36-37	0.4003938958939486
2202	38-39	0.40259036098949963
2202	40-41	0.22279341033872413
2202	42-43	0.1762417521579991
2202	44-45	0.1438407325677602
2202	46-47	0.059952395743174236
2202	48-49	-0.1830686241618622
2202	50-51	0.03982218502537194
2202	52-53	0.08564199677593365
2202	54-55	-0.028303986742415077
2202	56-57	0.059520444571717235
2202	58-59	0.06992475870340087
2202	60-61	0.01898648285651916
2202	62-63	0.02247398748252749
2202	64-65	-0.11765044864400664
2202	66-67	0.022287321195264553
2202	68-69	0.28849557781874324
2202	70-71	0.08112755403715255
2202	72-73	0.0037862907873318363
2202	74-75	-0.09406641578083708
2202	76-77	-0.07450393881948969
2202	78-79	0.10963502766651345
2202	80-81	0.16595502914978866
2202	82-83	0.17948401445192275
2202	84-85	0.2585533099549444
2202	86-87	0.26256430394334274
2202	88-89	0.13232326206740197
2202	90-91	0.023909787681944294
2202	92-93	0.15478565349399886
2202	94-95	0.25193582552168436
2202	96-97	0.23847881834251083
2202	98-99	0.4265520016155371
2202	100-101	0.3296675471223658
1102	1	0.11023190608447209
1102	2	0.09936938480142743
1102	3	0.10896805374612484
1102	4	0.10382435422862102
1102	5	0.19210611880668438
1102	6	0.17877764015564424
1102	7	0.13989524699604772
1102	8	0.16947533267372705
1102	9	0.18818615016598983
1102	10-11	0.14775340120579017
1102	12-13	0.15508412778951453
1102	14-15	0.31239002885743616
1102	16-17	0.30468732271461363
1102	18-19	0.3045943805679059
1102	20-21	0.36941984455593513
1102	22-23	0.23922787578919014
1102	24-25	0.24683068559171772
1102	26-27	0.3391281926017342
1102	28-29	0.19035937935678504
1102	30-31	0.24874062978839362
1102	32-33	0.22442855906773929
1102	34-35	0.32711974183286685
1102	36-37	0.488111507254942
1102	38-39	0.44392183305057387
1102	40-41	0.22080238456489099
1102	42-43	0.25210345380338595
1102	44-45	0.18237817047151594
1102	46-47	0.199356849689714
1102	48-49	-0.12936750164368505
1102	50-51	0.0029493455774840527
1102	52-53	0.17048770778123412
1102	54-55	0.11617584003994352
1102	56-57	0.14791555058132388
1102	58-59	0.12030593899524433
1102	60-61	-0.06797005981849935
1102	62-63	-0.01623546072917037
1102	64-65	-0.30724805304284786
1102	66-67	-0.10178240087995505
1102	68-69	0.11153040761590916
1102	70-71	-0.03934631169427405
1102	72-73	-0.22527409086742978
1102	74-75	-0.12915710582569062
1102	76-77	-0.0030205959613525124
1102	78-79	0.27509909024321644
1102	80-81	0.10837270531706977
1102	82-83	0.10917122487598618
1102	84-85	0.10752437215088761
1102	86-87	0.11561283020515489
1102	88-89	0.12123654824881669
1102	90-91	0.0052946221651879455
1102	92-93	0.20240230187443942
1102	94-95	0.3356968709534982
1102	96-97	0.16230982120982063
1102	98-99	0.2252987551353094
1102	100-101	0.1868259405935362
2203	1	0.039442303878708174
2203	2	0.030556417434823402
2203	3	0.049069853262594165
2203	4	0.08098040603017864
2203	5	0.015586991985038878
2203	6	0.05498409230909829
2203	7	0.09576721817379763
2203	8	0.0901327630793034
2203	9	0.062137909498439114
2203	10-11	0.034236445092915346
2203	12-13	0.07366197729954393
2203	14-15	0.05076387143088823
2203	16-17	0.06277828963363419
2203	18-19	0.1246629194019846
2203	20-21	0.060374647731414655
2203	22-23	0.04445433396197984
2203	24-25	0.050194336086661906
2203	26-27	0.13494778674865415
2203	28-29	-0.05198070864015136
2203	30-31	-0.06184386303856115
2203	32-33	0.00936914294320701
2203	34-35	0.1454729797608465
2203	36-37	0.25553119709384475
2203	38-39	0.2143856635743262
2203	40-41	-0.0218946962782951
2203	42-43	-0.28975034692136603
2203	44-45	-0.3277672637088145
2203	46-47	-0.2724481900147353
2203	48-49	-0.5959580226248136
2203	50-51	-0.4526842004169538
2203	52-53	-0.24134225814228216
2203	54-55	-0.35369330006614064
2203	56-57	-0.2895674304665974
2203	58-59	-0.24622535878873464
2203	60-61	-0.45733368605971947
2203	62-63	-0.3120202287257072
2203	64-65	-0.4690134709731879
2203	66-67	-0.3515110236980803
2203	68-69	-0.09789054562179444
2203	70-71	-0.3232761875482524
2203	72-73	-0.49661397711744115
2203	74-75	-0.3167907576545801
2203	76-77	-0.3303786592790203
2203	78-79	-0.17653665843081967
2203	80-81	-0.15833019862603592
2203	82-83	-0.12275866641218514
2203	84-85	-0.16427490178065085
2203	86-87	-0.15149661033848005
2203	88-89	-0.24055376282730734
2203	90-91	-0.41994157057258974
2203	92-93	-0.2929306852920277
2203	94-95	-0.21865890038675317
2203	96-97	-0.34850384731005235
2203	98-99	-0.19962910655669575
2203	100-101	-0.2157226743531524
1103	1	0.019589491866582875
1103	2	0.04253512306529217
1103	3	0.034081159722418874
1103	4	0.05201349218905449
1103	5	0.058156103123351954
1103	6	0.07956371319373545
1103	7	0.11680148544433422
1103	8	0.0648485554012197
1103	9	0.07962854113548445
1103	10-11	0.052413817999934054
1103	12-13	0.07319015540259244
1103	14-15	0.16311890071172286
1103	16-17	0.13318388337846443
1103	18-19	0.1965434639159369
1103	20-21	0.20694472093886418
1103	22-23	0.13256236487703887
1103	24-25	0.11738807920131222
1103	26-27	0.0843476746504166
1103	28-29	0.058673949342122
1103	30-31	0.11271252215846062
1103	32-33	0.13865051165834785
1103	34-35	0.13492436413109488
1103	36-37	0.2653591198620262
1103	38-39	0.23190556530584416
1103	40-41	0.24821342912569833
1103	42-43	0.111469591102491
1103	44-45	-0.03897824675879491
1103	46-47	0.046403103732025386
1103	48-49	-0.2190860787512463
1103	50-51	0.09773904557854252
1103	52-53	0.12425702717779075
1103	54-55	0.10790996237902561
1103	56-57	0.14082912240533574
1103	58-59	0.13219334744302103
1103	60-61	-0.011780227872144167
1103	62-63	-0.0372638276373678
1103	64-65	-0.20113925721773995
1103	66-67	0.021326650757472976
1103	68-69	0.18194738438338476
1103	70-71	0.05197789104506256
1103	72-73	-0.18713645796732692
1103	74-75	-0.18531857712918054
1103	76-77	-0.019148370422058747
1103	78-79	0.09837695935715374
1103	80-81	0.08984783999846613
1103	82-83	0.16056130962198267
1103	84-85	0.07992536751281065
1103	86-87	0.12003402447682276
1103	88-89	0.06539867487978768
1103	90-91	-0.04818091962973625
1103	92-93	0.13558905967375168
1103	94-95	0.1561551389507123
1103	96-97	0.0994939966242967
1103	98-99	0.25772526774403204
1103	100-101	0.12711369014220253
2204	1	0.03658617700597944
2204	2	0.03268653522817999
2204	3	0.039228273826608984
2204	4	0.06529370398859413
2204	5	0.10613567223953169
2204	6	0.08606143731672233
2204	7	0.06902493265905463
2204	8	0.081929090447737
2204	9	0.11129359603013711
2204	10-11	0.10648529313694155
2204	12-13	0.1301432308661319
2204	14-15	0.22957205478991227
2204	16-17	0.09692102996870489
2204	18-19	0.1615961400239172
2204	20-21	0.19266107807321475
2204	22-23	0.0470271776111062
2204	24-25	0.12268362566411639
2204	26-27	0.1724104173817409
2204	28-29	-0.0018937050884630935
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1213	58-59	-1.2640080214963945
1213	60-61	-1.3081567119715842
1213	62-63	-1.5078631633697768
1213	64-65	-1.4027141793578295
1213	66-67	-1.5544101946762154
1213	68-69	-1.5182115806604415
1213	70-71	-1.6972848619390533
1213	72-73	-1.7232417358860843
1213	74-75	-1.742066463974755
1213	76-77	-1.7278969433581395
1213	78-79	-2.011079385909177
1213	80-81	-1.5959260711096164
1213	82-83	-1.7819653869108194
1213	84-85	-1.6217816606964952
1213	86-87	-1.7046112228811623
1213	88-89	-1.7238049000844633
1213	90-91	-1.9269969272602339
1213	92-93	-2.188164150632165
1213	94-95	-1.9098145583084154
1213	96-97	-2.1095412915243124
1213	98-99	-2.068387943412805
1213	100-101	-2.122336286971233
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	45.0
9	492.0
10	3949.0
11	5296.0
12	4705.0
13	6677.0
14	8290.0
15	6089.0
16	7515.0
17	12722.0
18	17775.0
19	23227.0
20	28396.0
21	34829.0
22	40292.0
23	46833.0
24	53585.0
25	59039.0
26	68426.0
27	84120.0
28	104182.0
29	104262.0
30	101789.0
31	112737.0
32	138170.0
33	179112.0
34	240341.0
35	318294.0
36	520737.0
37	1411873.0
38	786745.0
39	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.57568301758499	17.928920472921586	18.224590288793003	35.270806220700415
2	19.857981635175705	24.95419830478545	34.70578756842663	20.48203249161222
3	20.01863724680929	24.801711571275327	28.61080099319173	26.568850188723648
4	22.35780531523492	29.632742592266826	21.741688861853202	26.267763230645052
5	25.987911818919642	31.072205284093002	22.533909305753994	20.405973591233366
6	20.766241038511804	32.69407811749209	24.635917642486035	21.903763201510074
7	16.17498819702395	23.02857192046063	37.93532785787076	22.861112024644658
8	19.106982086624903	25.433884904043296	27.257888901715145	28.201244107616656
9	25.01767411514098	22.737840175675075	27.896673249258324	24.347812459925613
10-11	23.305585947406513	32.37056952477102	22.26273038427047	22.061114143552
12-13	20.245682327550245	26.587009216949937	27.0489934370231	26.118315018476718
14-15	21.398353415059635	28.165886165377835	26.868719620680015	23.567040798882513
16-17	23.282417431667024	26.892151531709523	25.869669079932443	23.955761956691006
18-19	21.85454274972813	25.960819637503512	26.89148347130878	25.293154141459578
20-21	22.94714676634623	27.813919712262454	26.376324558949392	22.86260896244193
22-23	22.057475193003587	29.416750342981544	24.903433159204667	23.622341304810202
24-25	21.47112639685747	25.82469794014401	27.273618330650944	25.430557332347576
26-27	20.909543886504213	28.66135268940948	24.538741860207352	25.89036156387895
28-29	22.80229472811912	28.32573558966695	25.03676398979373	23.8352056924202
30-31	21.902736258920456	26.074265176139384	26.490978944418032	25.532019620522128
32-33	21.865616133595545	28.078542488856208	24.908670748198787	25.147170629349464
34-35	20.557830763449576	27.888097800670188	26.34024122999285	25.21383020588739
36-37	21.756412828849356	29.242318766958462	26.021871100404802	22.97939730378738
38-39	23.21634734481454	26.424245242208038	25.80606515929978	24.55334225367764
40-41	20.27205313323033	28.28168449521548	27.12514538285572	24.32111698869847
42-43	20.597181788344756	28.30461692603185	26.910007589733446	24.188193695889947
44-45	22.80899063873712	27.933837138941787	25.54697093737208	23.71020128494901
46-47	21.102869139885115	27.751419967980944	27.464856471339676	23.68085442079427
48-49	21.80425566490411	26.910582553398942	26.367764735715195	24.917397045981755
50-51	20.75451270955394	27.102775778952815	25.141211621337682	27.001499890155568
52-53	22.658366170469385	28.306582258094267	25.857506998932443	23.1775445725039
54-55	21.53907023146722	25.71328842605924	27.187507211507107	25.560134130966432
56-57	20.82671271204981	25.708666064016917	28.173560007208614	25.291061216724657
58-59	22.221498856679876	28.76614042389573	25.960601763643187	23.051758955781203
60-61	21.249320990828068	25.45092915279774	26.542585890468835	26.757163965905356
62-63	22.204460664899972	27.807654891876176	27.406604327092836	22.581280116131012
64-65	21.550203433051472	29.73639188770898	26.0586173257242	22.654787353515353
66-67	21.896219287255477	29.883940528334048	23.868747660450033	24.35109252396044
68-69	20.836439501635244	30.378666726248493	25.457423663109445	23.327470109006818
70-71	21.06975265336186	28.361114214589122	25.56339868754754	25.00573444450148
72-73	21.349907891152125	28.77694106879829	25.693831644158323	24.17931939589127
74-75	21.530658845856184	28.42815845744057	26.59967584798662	23.441506848716624
76-77	21.82010030692134	27.32649574035707	25.44346597218562	25.409937980535968
78-79	22.394471410684005	27.037012563753937	25.090672269472254	25.4778437560898
80-81	23.466179002287316	27.028385360722453	25.313544709512197	24.19189092747803
82-83	22.13286865006059	27.367471025848367	26.320939268297867	24.178721055793183
84-85	22.913868361418245	28.38724650365195	24.377349647860928	24.321535487068875
86-87	21.756484782980557	28.3374258553178	24.843611857519168	25.06247750418248
88-89	21.492409048944687	29.215159764722127	25.335824521447876	23.956606664885317
90-91	22.840503812700323	29.020601034595334	24.639153745443775	23.499741407260565
92-93	21.224207190403884	27.796116421566968	27.12400550294765	23.855670885081494
94-95	20.946086007550395	28.567313577935405	25.224859811534273	25.261740602979927
96-97	20.544287274902725	29.87477391621515	24.722269590999826	24.858669217882294
98-99	21.297417031660938	30.484174612430557	25.059799976178233	23.15860837973027
100-101	21.034435983410948	30.565347657291664	24.475741521343792	23.924474837953596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1536.0
1	985.5
2	346.0
3	265.0
4	257.0
5	240.5
6	281.0
7	377.5
8	510.5
9	722.0
10	1003.0
11	1354.0
12	1907.5
13	2482.0
14	2848.0
15	3217.5
16	3534.5
17	3725.5
18	3879.5
19	3864.0
20	3835.0
21	3993.0
22	4144.5
23	4364.5
24	4847.0
25	5850.0
26	7612.5
27	9144.0
28	20174.0
29	22365.0
30	12998.5
31	13613.0
32	15076.5
33	18516.0
34	23429.0
35	25168.5
36	36672.5
37	58349.0
38	81415.5
39	96471.0
40	115663.5
41	160576.5
42	215258.0
43	232419.5
44	244938.5
45	290557.5
46	337077.5
47	387542.0
48	402085.0
49	361325.0
50	291304.0
51	218946.0
52	171525.5
53	192203.5
54	174680.0
55	90350.5
56	48215.0
57	25866.0
58	13866.5
59	8997.5
60	6525.0
61	6273.0
62	8327.0
63	8006.0
64	5220.5
65	3039.5
66	1989.0
67	1467.5
68	775.0
69	601.0
70	434.0
71	352.0
72	314.0
73	376.0
74	406.0
75	351.5
76	272.0
77	278.0
78	235.0
79	97.5
80	76.0
81	53.0
82	52.0
83	59.5
84	56.5
85	55.5
86	43.0
87	41.0
88	47.5
89	49.0
90	52.5
91	46.5
92	39.5
93	35.5
94	25.0
95	14.0
96	11.5
97	13.5
98	21.5
99	35.5
100	242.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020350339256930097
2	0.005231052498798734
3	0.022027807568781168
4	0.0
5	0.0021189073412855633
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0015671085544924476
14-15	0.002394806734682121
16-17	0.004248850658306989
18-19	0.0
20-21	0.0026375982008710916
22-23	0.3249763774939374
24-25	0.017249230075152788
26-27	0.13306075944729184
28-29	0.27874667513641016
30-31	0.22663479771166836
32-33	0.8288679936176746
34-35	0.5586410917493501
36-37	1.1081002516864626
38-39	0.7872734010692094
40-41	1.5450586749721948
42-43	1.108950021818124
44-45	0.7839295004212432
46-47	1.445348634198679
48-49	1.120659192073874
50-51	2.2435255792949205
52-53	1.8752109250862519
54-55	1.0068672462613977
56-57	0.9786372203290619
58-59	2.5283641130375267
60-61	1.26267012784295
62-63	0.9859320002904669
64-65	1.2402670970991496
66-67	0.7632480818922373
68-69	1.213946294969118
70-71	1.3209290437525671
72-73	1.362711247888542
74-75	1.478611065066568
76-77	2.009938116869659
78-79	1.4613176910884718
80-81	2.701915867459697
82-83	2.335896696204088
84-85	1.9470992710296589
86-87	2.7733738103494057
88-89	1.9905699794532203
90-91	1.6293956015456548
92-93	2.2295098901103754
94-95	2.8388171464630694
96-97	1.695401772421847
98-99	2.2494518982650784
100-101	2.289777353603919
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4530637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.135926013555904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.57385458868481	7.635245521899041
2	6.595863139528394	1.2051863527654396
3	1.6012252968038032	0.4388602752790093
4	1.0093179932004632	0.36884218040120614
5	0.5849291417870844	0.26719346812697775
6	0.25841606970544323	0.1416522056125701
7	0.34503399625289577	0.22065435433495867
8	0.1511622331144008	0.1104805582221645
9	0.16338596012596193	0.1343413839028125
>10	1.6835651782053078	3.364445397523329
>50	0.8801459274954003	6.27291222185488
>100	2.947499559762765	48.19452544970341
>500	0.11329014928741911	7.23886366403453
>1k	0.08445022853608432	14.890165462867285
>5k	0.004287566097211429	2.7395029484138433
>10k+	0.003572971412494536	6.777128555058501
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT	68431	1.5104057111615872	No Hit
CACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAAC	31968	0.7055961446480925	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	29542	0.6520495903776886	TruSeq Adapter, Index 2 (100% over 50bp)
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA	24299	0.5363263488114364	No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA	22731	0.5017175289037722	No Hit
GGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAACCATTGCG	17528	0.3868771653963891	No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA	15084	0.3329333159994941	No Hit
CTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCT	14486	0.3197342890194028	No Hit
CCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTAC	14101	0.3112365877027888	No Hit
CTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCTT	13566	0.2994280936654162	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	13014	0.2872443764530242	TruSeq Adapter, Index 3 (100% over 50bp)
CAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTC	12318	0.2718822982287038	No Hit
AAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCA	11895	0.2625458627561643	No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG	11337	0.25022971383494197	No Hit
CTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACT	11126	0.24557253207440807	No Hit
CTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAAC	9991	0.22052086715400065	No Hit
CTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCAAGATC	9513	0.20997047435051627	No Hit
CTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAACCATTGCGACCCT	9475	0.20913174019459072	No Hit
ATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACG	9465	0.20891102067987347	No Hit
GTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGC	9386	0.20716733651360725	No Hit
ACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAG	7588	0.16748196776744637	No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA	6529	0.14410777115889004	No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC	6398	0.1412163455160941	No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA	6394	0.1411280577102072	No Hit
TACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAA	6153	0.13580871740552156	No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC	6076	0.13410917714219878	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	5951	0.1313501832082332	No Hit
CAAGGATCTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATG	5738	0.12664885754475583	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	5719	0.12622949046679308	No Hit
CACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTC	5538	0.12223446725041093	No Hit
CTTCAACGCTAACCATTGCGACCCTCTTACTCGTTGCGGCCAGATTTCCC	5395	0.11907817818995431	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	5336	0.11777593305312255	No Hit
GGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTA	5242	0.11570116961478044	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	4989	0.1101169658924341	No Hit
TTAACGTTCACATCGAGATGTAACTTTATTGCATACAATATTATAATATT	4835	0.10671788536578852	No Hit
TTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCA	4819	0.10636473414224093	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC	4747	0.10477555363627676	TruSeq Adapter, Index 2 (98% over 50bp)
CCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCAAG	4742	0.10466519387891814	No Hit
TCTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCT	4714	0.10404717923770984	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	4693	0.10358366825680362	No Hit
CCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAA	4692	0.1035615963053319	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTATCTCGTATGCC	4669	0.10305394142148223	TruSeq Adapter, Index 9 (97% over 36bp)
TTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCAC	4656	0.10276700605234981	No Hit
AAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCACATTCAACT	4645	0.10252421458616084	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	4642	0.10245799873174566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0019202597780400416	2.2071951471724616E-5	0.0
2	0.001964403680983491	2.2071951471724616E-5	0.0
3	0.002052691486870389	2.2071951471724616E-5	0.0
4	0.002185123195700737	2.2071951471724616E-5	0.0
5	0.0023837707589462586	2.2071951471724616E-5	0.0
6	0.0024279146618897077	2.2071951471724616E-5	0.0
7	0.002472058564833157	2.2071951471724616E-5	0.0
8	0.0026265622251352293	2.2071951471724616E-5	0.0
9	0.0032004329634000694	2.2071951471724616E-5	0.0
10-11	0.003983987240646293	2.2071951471724616E-5	0.0
12-13	0.004292994561250438	2.2071951471724616E-5	0.0
14-15	0.0050103329840814875	2.2071951471724616E-5	0.0
16-17	0.005462807989251842	2.2071951471724616E-5	0.0
18-19	0.005893211042950472	2.2071951471724616E-5	0.0
20-21	0.006301542145177377	2.2071951471724616E-5	0.0
22-23	0.006754017150347733	2.2071951471724616E-5	0.0
24-25	0.007283743985669123	2.2071951471724616E-5	0.0
26-27	0.008199729971745695	2.2071951471724616E-5	0.0
28-29	0.010362781215974707	2.2071951471724616E-5	0.0
30-31	0.013265242834506495	2.2071951471724616E-5	0.0
32-33	0.01553865383609413	3.310792720758692E-5	0.0
34-35	0.019335029489230764	4.414390294344923E-5	0.0
36-37	0.024223966740217765	4.414390294344923E-5	0.0
38-39	0.029775062535356507	4.414390294344923E-5	0.0
40-41	0.03625318029230769	4.414390294344923E-5	0.0
42-43	0.043393456593410595	4.414390294344923E-5	0.0
44-45	0.05181390607987354	4.414390294344923E-5	0.0
46-47	0.06203321961128204	4.414390294344923E-5	0.0
48-49	0.07447076426559884	4.414390294344923E-5	0.0
50-51	0.08933622358180537	4.414390294344923E-5	0.0
52-53	0.1065744176812223	4.414390294344923E-5	0.0
54-55	0.1274544837734738	4.414390294344923E-5	0.0
56-57	0.1477717151031963	4.414390294344923E-5	0.0
58-59	0.16901596839473124	4.414390294344923E-5	0.0
60-61	0.19317371928053384	4.414390294344923E-5	0.0
62-63	0.22355576048136278	4.414390294344923E-5	0.0
64-65	0.2603497035847277	4.414390294344923E-5	0.0
66-67	0.2969449991248471	4.414390294344923E-5	0.0
68-69	0.33676279957983835	4.414390294344923E-5	0.0
70-71	0.38035490373649444	4.414390294344923E-5	0.0
72-73	0.43482847996871077	4.414390294344923E-5	0.0
74-75	0.49968690936837357	4.414390294344923E-5	0.0
76-77	0.5634858850973936	4.414390294344923E-5	0.0
78-79	0.6365550804445379	4.414390294344923E-5	0.0
80-81	0.7237282527821143	4.414390294344923E-5	0.0
82-83	0.8202820044951736	4.414390294344923E-5	0.0
84-85	0.925653500821187	4.414390294344923E-5	0.0
86-87	1.040262108838117	6.621585441517385E-5	0.0
88-89	1.1699017158072915	6.621585441517385E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCAAT	495	0.0	73.43458	1
TCCAATC	550	0.0	64.38581	2
CGGAAGA	22370	0.0	63.449863	4
TCGGAAG	22685	0.0	62.70517	3
AGAGCAC	22545	0.0	62.341618	8
AAGAGCA	22705	0.0	62.266808	7
GCATTCC	425	0.0	61.392372	3
GAGCACA	23275	0.0	60.32628	9
GATCGGA	23640	0.0	60.244457	1
GAAGAGC	24660	0.0	57.365658	6
GGAAGAG	25395	0.0	55.855164	5
ATCGGAA	26870	0.0	52.90708	2
GGCCCTC	345	0.0	52.6726	2
CCAGACG	285	0.0	52.300446	6
TCAAAGG	740	0.0	50.98856	7
CAATCAA	770	0.0	47.792595	4
CTTGTTA	680	0.0	46.60271	1
GATCTAC	125	0.0	44.71935	5
GTCACCG	7210	0.0	43.917103	28-29
GCTTCAG	330	0.0	43.759464	5
>>END_MODULE