FastQCFastQC Report
Thu 14 Aug 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4530637
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT684311.5104057111615872No Hit
CACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAAC319680.7055961446480925No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC295420.6520495903776886TruSeq Adapter, Index 2 (100% over 50bp)
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA242990.5363263488114364No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA227310.5017175289037722No Hit
GGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAACCATTGCG175280.3868771653963891No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA150840.3329333159994941No Hit
CTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCT144860.3197342890194028No Hit
CCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTAC141010.3112365877027888No Hit
CTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCTT135660.2994280936654162No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC130140.2872443764530242TruSeq Adapter, Index 3 (100% over 50bp)
CAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTC123180.2718822982287038No Hit
AAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCA118950.2625458627561643No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG113370.25022971383494197No Hit
CTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACT111260.24557253207440807No Hit
CTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAAC99910.22052086715400065No Hit
CTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCAAGATC95130.20997047435051627No Hit
CTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAACCATTGCGACCCT94750.20913174019459072No Hit
ATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACG94650.20891102067987347No Hit
GTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGC93860.20716733651360725No Hit
ACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAG75880.16748196776744637No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA65290.14410777115889004No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC63980.1412163455160941No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA63940.1411280577102072No Hit
TACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAA61530.13580871740552156No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC60760.13410917714219878No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG59510.1313501832082332No Hit
CAAGGATCTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATG57380.12664885754475583No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA57190.12622949046679308No Hit
CACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTC55380.12223446725041093No Hit
CTTCAACGCTAACCATTGCGACCCTCTTACTCGTTGCGGCCAGATTTCCC53950.11907817818995431No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC53360.11777593305312255No Hit
GGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTA52420.11570116961478044No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG49890.1101169658924341No Hit
TTAACGTTCACATCGAGATGTAACTTTATTGCATACAATATTATAATATT48350.10671788536578852No Hit
TTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCA48190.10636473414224093No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC47470.10477555363627676TruSeq Adapter, Index 2 (98% over 50bp)
CCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCAAG47420.10466519387891814No Hit
TCTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCT47140.10404717923770984No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG46930.10358366825680362No Hit
CCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAA46920.1035615963053319No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTATCTCGTATGCC46690.10305394142148223TruSeq Adapter, Index 9 (97% over 36bp)
TTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCAC46560.10276700605234981No Hit
AAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCACATTCAACT46450.10252421458616084No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC46420.10245799873174566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT4950.073.434581
TCCAATC5500.064.385812
CGGAAGA223700.063.4498634
TCGGAAG226850.062.705173
AGAGCAC225450.062.3416188
AAGAGCA227050.062.2668087
GCATTCC4250.061.3923723
GAGCACA232750.060.326289
GATCGGA236400.060.2444571
GAAGAGC246600.057.3656586
GGAAGAG253950.055.8551645
ATCGGAA268700.052.907082
GGCCCTC3450.052.67262
CCAGACG2850.052.3004466
TCAAAGG7400.050.988567
CAATCAA7700.047.7925954
CTTGTTA6800.046.602711
GATCTAC1250.044.719355
GTCACCG72100.043.91710328-29
GCTTCAG3300.043.7594645
Produced by FastQC (version 0.11.2)