##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane1_Undetermined_L001_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4528098 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.9504814162591 33.0 33.0 33.0 22.0 33.0 2 29.840151648661315 33.0 33.0 33.0 15.0 33.0 3 29.80736636000369 33.0 33.0 33.0 15.0 33.0 4 33.22868012132246 37.0 37.0 37.0 15.0 37.0 5 33.25688401620283 37.0 37.0 37.0 15.0 37.0 6 33.32801520638467 37.0 37.0 37.0 22.0 37.0 7 33.21938195683927 37.0 37.0 37.0 15.0 37.0 8 33.394027249410236 37.0 37.0 37.0 22.0 37.0 9 33.306347389124525 37.0 37.0 37.0 15.0 37.0 10-11 33.45551421369414 37.0 37.0 37.0 22.0 37.0 12-13 33.45768388846708 37.0 37.0 37.0 22.0 37.0 14-15 35.076256520949855 40.0 37.0 40.0 22.0 40.0 16-17 35.00722422527074 40.0 37.0 40.0 22.0 40.0 18-19 34.95182900193414 40.0 37.0 40.0 22.0 40.0 20-21 34.84440321300467 40.0 37.0 40.0 22.0 40.0 22-23 34.882075100848084 38.5 33.0 40.0 22.0 40.0 24-25 34.580573123638224 37.0 33.0 40.0 15.0 40.0 26-27 34.436116665319524 37.0 33.0 40.0 18.5 40.0 28-29 33.991528562323516 37.0 33.0 40.0 15.0 40.0 30-31 33.87356126126245 37.0 33.0 40.0 15.0 40.0 32-33 33.73473498144254 37.0 33.0 40.0 15.0 40.0 34-35 33.76525408681526 37.0 33.0 40.0 15.0 40.0 36-37 33.76144542366353 37.0 33.0 40.0 15.0 40.0 38-39 33.70938835687743 37.0 33.0 40.0 15.0 40.0 40-41 33.61541645079237 37.0 33.0 40.0 15.0 40.0 42-43 33.31624624732062 37.0 33.0 40.0 15.0 40.0 44-45 33.04422662672054 37.0 33.0 40.0 15.0 40.0 46-47 32.77557354103202 37.0 33.0 40.0 15.0 40.0 48-49 32.459224380744416 37.0 33.0 40.0 15.0 40.0 50-51 32.00515326744253 37.0 27.0 40.0 15.0 40.0 52-53 31.883445322959 37.0 27.0 40.0 15.0 40.0 54-55 31.593978752226654 37.0 27.0 38.5 15.0 40.0 56-57 31.22711809682564 37.0 27.0 37.0 15.0 40.0 58-59 30.965455363377735 37.0 27.0 37.0 10.5 40.0 60-61 31.07085336933962 37.0 27.0 37.0 15.0 40.0 62-63 30.865692615310003 37.0 27.0 37.0 15.0 40.0 64-65 30.821694230116044 37.0 27.0 37.0 15.0 40.0 66-67 30.774107141674055 37.0 27.0 37.0 15.0 37.0 68-69 30.424637894321194 37.0 27.0 37.0 15.0 37.0 70-71 30.20418330168649 33.0 27.0 37.0 15.0 37.0 72-73 29.936576902708378 33.0 27.0 37.0 15.0 37.0 74-75 29.780513473869163 33.0 27.0 37.0 15.0 37.0 76-77 29.735454930524913 33.0 27.0 37.0 15.0 37.0 78-79 29.501829024018473 33.0 27.0 37.0 15.0 37.0 80-81 29.38603073520052 33.0 27.0 37.0 6.0 37.0 82-83 29.36717745508158 33.0 27.0 37.0 15.0 37.0 84-85 29.215938789310655 33.0 27.0 37.0 6.0 37.0 86-87 29.044940502612796 33.0 27.0 37.0 6.0 37.0 88-89 28.8767747517832 33.0 24.5 37.0 6.0 37.0 90-91 28.708699767540367 33.0 22.0 37.0 6.0 37.0 92-93 28.642143787524034 33.0 22.0 37.0 6.0 37.0 94-95 28.58390266730093 33.0 22.0 37.0 6.0 37.0 96-97 28.481494548042026 33.0 22.0 37.0 6.0 37.0 98-99 28.458975711214734 33.0 22.0 37.0 6.0 37.0 100-101 27.939711552179304 33.0 22.0 37.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.6837682243852079 2201 2 0.8441837936426637 2201 3 0.7738267779858603 2201 4 0.9162852995979804 2201 5 0.9379811413483239 2201 6 0.9153530049656595 2201 7 0.7382050848632886 2201 8 0.8071942154679306 2201 9 0.8237063804946985 2201 10-11 0.520015060948694 2201 12-13 0.30337252651541036 2201 14-15 0.6045800788359017 2201 16-17 0.8034254682292499 2201 18-19 0.6633468015540345 2201 20-21 0.6446613718037497 2201 22-23 0.6020161989503094 2201 24-25 0.7163354404926423 2201 26-27 0.819007047767883 2201 28-29 1.0123845571405639 2201 30-31 1.2742403830833169 2201 32-33 1.1959387437479023 2201 34-35 1.1355120203450824 2201 36-37 1.1044434856772227 2201 38-39 1.0002478602202416 2201 40-41 1.0566235590455406 2201 42-43 1.030762015837496 2201 44-45 1.0586476611424374 2201 46-47 0.9897806335320922 2201 48-49 1.1167703138597886 2201 50-51 1.0372176928405423 2201 52-53 1.0043859105382005 2201 54-55 1.035047952713466 2201 56-57 1.0069700891501014 2201 58-59 0.9427594143651774 2201 60-61 0.8947353051838327 2201 62-63 0.834823144890283 2201 64-65 0.8067837872590573 2201 66-67 0.7724569975086162 2201 68-69 0.8839261559035947 2201 70-71 0.8282841343734937 2201 72-73 0.8732691888702782 2201 74-75 0.8176238678300791 2201 76-77 0.8621191736772218 2201 78-79 0.7463501993412294 2201 80-81 0.8182382039162448 2201 82-83 0.7817563549109359 2201 84-85 0.7245381498834362 2201 86-87 0.8211958247135911 2201 88-89 0.8425597021019371 2201 90-91 0.8357249413264611 2201 92-93 0.7370242659623365 2201 94-95 0.6858970300083307 2201 96-97 0.8089087454806148 2201 98-99 0.805418992227505 2201 100-101 0.9685311596530362 1101 1 1.2251435311694365 1101 2 1.237802681695566 1101 3 1.2737803847587905 1101 4 1.4459788879578426 1101 5 1.3340901197598498 1101 6 1.3282904654532928 1101 7 1.3172464036809117 1101 8 1.2768205937434018 1101 9 1.1368885472266683 1101 10-11 1.0358137576114999 1101 12-13 1.0379711770253763 1101 14-15 1.2705401902757956 1101 16-17 1.2555870801174507 1101 18-19 1.280249080110515 1101 20-21 1.3407555668812634 1101 22-23 1.3787977108887475 1101 24-25 1.431751212747571 1101 26-27 1.4523623357544366 1101 28-29 1.724717102830681 1101 30-31 1.703003823297351 1101 32-33 1.631678595529948 1101 34-35 1.4951822438258233 1101 36-37 1.4775416638416417 1101 38-39 1.5405116525901192 1101 40-41 1.573966693448881 1101 42-43 1.4442747042649131 1101 44-45 1.4931421071760624 1101 46-47 1.546943819905465 1101 48-49 1.556849615028753 1101 50-51 1.4872348799687032 1101 52-53 1.4703775116814448 1101 54-55 1.4615731000059533 1101 56-57 1.3938655649691327 1101 58-59 1.4259437925877663 1101 60-61 1.2792189341437812 1101 62-63 1.3659002369724256 1101 64-65 1.3481595748021178 1101 66-67 1.351077532160513 1101 68-69 1.159583052749575 1101 70-71 1.226366988350211 1101 72-73 1.2265478438993753 1101 74-75 1.2755877580343764 1101 76-77 1.2158825537964049 1101 78-79 1.2397083959187114 1101 80-81 1.2466253301563022 1101 82-83 1.2672015015303586 1101 84-85 1.174434305976213 1101 86-87 1.1624889217379355 1101 88-89 1.0708339702067136 1101 90-91 1.1673057484724616 1101 92-93 1.254994069500242 1101 94-95 1.2161019053836206 1101 96-97 1.2765951416883894 1101 98-99 1.1378781457554332 1101 100-101 1.2930265431663734 2202 1 0.9286320046983754 2202 2 0.9815084244699079 2202 3 0.9772883147410916 2202 4 1.1210841076538287 2202 5 0.9701671124682889 2202 6 1.1332832325226647 2202 7 0.9273959110402004 2202 8 1.0436026594797312 2202 9 0.8945670259069587 2202 10-11 0.8502575382722597 2202 12-13 0.7996480396143539 2202 14-15 0.9676470771274239 2202 16-17 0.9611012588600829 2202 18-19 0.9645771906193872 2202 20-21 0.9237613241474634 2202 22-23 1.0839140725367002 2202 24-25 0.9922260187681502 2202 26-27 1.0161126581570912 2202 28-29 1.2630397201421033 2202 30-31 1.2988118559520387 2202 32-33 1.1872062141191648 2202 34-35 0.9917184750280654 2202 36-37 1.0565234104732326 2202 38-39 1.0467342424544626 2202 40-41 1.0693139769568276 2202 42-43 1.1228305484232166 2202 44-45 1.2003567752142104 2202 46-47 1.1580649442473288 2202 48-49 1.139721694876343 2202 50-51 1.0844625244919754 2202 52-53 1.086738024868449 2202 54-55 1.1070326186443147 2202 56-57 1.1165018363845292 2202 58-59 1.1718479254647036 2202 60-61 1.0682040756073299 2202 62-63 0.9391803325951003 2202 64-65 0.8961347324809452 2202 66-67 0.9490502666480047 2202 68-69 0.9013317907397074 2202 70-71 0.9291592494572356 2202 72-73 1.0092419432984485 2202 74-75 1.0656770000341211 2202 76-77 1.1448108010914702 2202 78-79 0.9815980809014704 2202 80-81 0.9596899366926763 2202 82-83 0.9288124060332166 2202 84-85 0.7903673563911227 2202 86-87 0.9379709535968708 2202 88-89 0.9429358038178677 2202 90-91 0.9339780968332825 2202 92-93 0.836820973732646 2202 94-95 0.9462757912297945 2202 96-97 0.9245090103570952 2202 98-99 1.0043319222577587 2202 100-101 0.9844735761045236 1102 1 0.9113960129562528 1102 2 0.9978248467678732 1102 3 0.8960683536541119 1102 4 1.0709111453807907 1102 5 1.0170392240173172 1102 6 1.1321748713822188 1102 7 1.0247596504156462 1102 8 0.9422715920338831 1102 9 0.8263927876763191 1102 10-11 0.736568956574061 1102 12-13 0.7212763250013765 1102 14-15 0.814699325837978 1102 16-17 0.7973458809042597 1102 18-19 0.8699489319218259 1102 20-21 0.898268928580066 1102 22-23 0.9496949019716823 1102 24-25 0.9299584055894456 1102 26-27 1.020557928721857 1102 28-29 1.2044760691657501 1102 30-31 1.2748433173634766 1102 32-33 1.1815185655473215 1102 34-35 1.0111367272520297 1102 36-37 0.9599703832453912 1102 38-39 0.9038185710127635 1102 40-41 1.0207355708362513 1102 42-43 1.0065508548540478 1102 44-45 1.0511545278939636 1102 46-47 0.9772663975838043 1102 48-49 0.9697447896419362 1102 50-51 1.0271587392664827 1102 52-53 0.9491504312054424 1102 54-55 0.778097460317138 1102 56-57 0.8336465814124523 1102 58-59 0.8847269898994483 1102 60-61 0.8338623903315536 1102 62-63 0.8646449443141435 1102 64-65 0.8216059216464089 1102 66-67 0.771130542092255 1102 68-69 0.6686518208061187 1102 70-71 0.6133112664161011 1102 72-73 0.7508165862039675 1102 74-75 0.7577457945400674 1102 76-77 0.7505930800214315 1102 78-79 0.6303255460284447 1102 80-81 0.6523140396501503 1102 82-83 0.7645492804820506 1102 84-85 0.8379433887124037 1102 86-87 0.7643458213639569 1102 88-89 0.7028162841344923 1102 90-91 0.6767847658055928 1102 92-93 0.6442092328009537 1102 94-95 0.7333345593176332 1102 96-97 0.8273886811676263 1102 98-99 0.7540121455737925 1102 100-101 0.9019605158532151 2203 1 0.6851634688212656 2203 2 0.7346491463841041 2203 3 0.6503893632212367 2203 4 0.8163088189728427 2203 5 0.7137002037569431 2203 6 0.6647444970330127 2203 7 0.6380504650946719 2203 8 0.48551936769551673 2203 9 0.485513419673687 2203 10-11 0.37617002769515295 2203 12-13 0.24504494633714557 2203 14-15 0.3565400789074431 2203 16-17 0.3687891421523375 2203 18-19 0.43553896378943335 2203 20-21 0.3705166319611237 2203 22-23 0.4929184486724125 2203 24-25 0.4551963113176214 2203 26-27 0.5007892351055148 2203 28-29 0.7199553813113297 2203 30-31 0.7258052263575365 2203 32-33 0.5965532880220081 2203 34-35 0.5322248299982206 2203 36-37 0.5105468528826407 2203 38-39 0.5081582174196271 2203 40-41 0.4825809451576575 2203 42-43 0.5013225102630443 2203 44-45 0.5675892266743077 2203 46-47 0.4795890311114732 2203 48-49 0.3658523204648958 2203 50-51 0.5292713635174238 2203 52-53 0.48480799836115196 2203 54-55 0.5119042171392074 2203 56-57 0.5160966126556161 2203 58-59 0.31376534607672113 2203 60-61 0.3713384233342616 2203 62-63 0.37472253472794037 2203 64-65 0.44419680805000183 2203 66-67 0.43431501023769314 2203 68-69 0.4661170929852432 2203 70-71 0.37997656802068747 2203 72-73 0.3648073783434356 2203 74-75 0.3834891617283631 2203 76-77 0.3240432284532204 2203 78-79 0.3652955923607166 2203 80-81 0.30629226181325464 2203 82-83 0.32957672143758643 2203 84-85 0.35285283911916565 2203 86-87 0.2898136456032674 2203 88-89 0.3254770346292304 2203 90-91 0.2926531634137497 2203 92-93 0.3193208613763261 2203 94-95 0.4114147916077151 2203 96-97 0.38268149019166486 2203 98-99 0.4010381956629452 2203 100-101 0.4529293855946399 1103 1 0.8477647721776762 1103 2 0.8708812489123829 1103 3 0.9314569070186458 1103 4 1.0245452307264813 1103 5 0.9568980034873533 1103 6 0.9144755873302302 1103 7 0.9501045316822285 1103 8 0.8826472845038893 1103 9 0.7617228088057217 1103 10-11 0.5674256286083903 1103 12-13 0.48999328549196264 1103 14-15 0.5608779669818063 1103 16-17 0.5550655988358528 1103 18-19 0.6307278563366978 1103 20-21 0.6270786873825784 1103 22-23 0.7097201378447267 1103 24-25 0.771606033488311 1103 26-27 0.7208812693001647 1103 28-29 1.0642344852786039 1103 30-31 1.0402609081792207 1103 32-33 0.9540849441614299 1103 34-35 0.6809428700560716 1103 36-37 0.6537064495510094 1103 38-39 0.6770654543485648 1103 40-41 0.6927429554891589 1103 42-43 0.7490455010442005 1103 44-45 0.7944353946495468 1103 46-47 0.6985115134133792 1103 48-49 0.603529904880773 1103 50-51 0.6372475200375902 1103 52-53 0.6430173878266991 1103 54-55 0.5804485103879777 1103 56-57 0.72988346366526 1103 58-59 0.6805964921277265 1103 60-61 0.696199051360253 1103 62-63 0.7496330510170601 1103 64-65 0.6252418860790492 1103 66-67 0.6587654618584189 1103 68-69 0.5665220583661146 1103 70-71 0.5019006670741746 1103 72-73 0.5490662722905846 1103 74-75 0.5039649816163561 1103 76-77 0.5236080787346538 1103 78-79 0.5131416717068618 1103 80-81 0.5463791737793784 1103 82-83 0.4740566770446648 1103 84-85 0.5129189791082922 1103 86-87 0.5979028313269268 1103 88-89 0.6022949671899056 1103 90-91 0.4175721424064811 1103 92-93 0.45974224190091917 1103 94-95 0.5374721605673365 1103 96-97 0.5424919669020127 1103 98-99 0.4967227650193031 1103 100-101 0.3940712090012788 2204 1 0.9209268407626894 2204 2 1.026412417389345 2204 3 1.0027251947251017 2204 4 1.1601300105244903 2204 5 1.115437651922008 2204 6 1.0747172442635744 2204 7 1.1180266609781597 2204 8 1.0157719604937228 2204 9 0.7923765754434484 2204 10-11 0.7960285488118473 2204 12-13 0.7535288428102689 2204 14-15 0.7229319468156206 2204 16-17 0.7682879098897786 2204 18-19 0.7022763698977528 2204 20-21 0.6198422520877997 2204 22-23 0.6083033519839631 2204 24-25 0.4525597320448611 2204 26-27 0.3278276413231822 2204 28-29 0.6223009279228222 2204 30-31 0.6599501707164634 2204 32-33 0.49724688782686144 2204 34-35 0.2452868132259951 2204 36-37 0.08502442402161847 2204 38-39 0.24103178643895262 2204 40-41 0.33530201642701485 2204 42-43 0.4183477106634328 2204 44-45 0.5526062596551995 2204 46-47 0.6183484413077096 2204 48-49 0.6364126845080591 2204 50-51 0.7162572408631434 2204 52-53 0.711265390674864 2204 54-55 0.7244669027145854 2204 56-57 0.6575497992950332 2204 58-59 0.709513283193477 2204 60-61 0.6512635202750552 2204 62-63 0.6539559244173141 2204 64-65 0.6802806944295057 2204 66-67 0.6279323106994781 2204 68-69 0.7297252671338015 2204 70-71 0.6737976334020992 2204 72-73 0.6771430416203295 2204 74-75 0.6264918028918238 2204 76-77 0.6480421097438551 2204 78-79 0.5606080319041489 2204 80-81 0.6068718878446973 2204 82-83 0.632112532754217 2204 84-85 0.6098876284569386 2204 86-87 0.6042168604206992 2204 88-89 0.6727180281777265 2204 90-91 0.750928710782258 2204 92-93 0.721324192270373 2204 94-95 0.6674342891137535 2204 96-97 0.6497210319413931 2204 98-99 0.6774191708746997 2204 100-101 0.6421066715889054 2205 1 0.7739821091754635 2205 2 0.8579123821019152 2205 3 0.8632680398880268 2205 4 1.0076393824498098 2205 5 0.929951910118028 2205 6 0.7818863623685175 2205 7 0.728626547228103 2205 8 0.474255763564976 2205 9 0.4533238560283124 2205 10-11 0.3033424283945365 2205 12-13 0.29444876526095953 2205 14-15 0.2959798878980777 2205 16-17 0.17537634433196558 2205 18-19 0.26309427017132947 2205 20-21 0.06075957987478375 2205 22-23 0.23137134462757558 2205 24-25 0.274117900253799 2205 26-27 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-0.31813875466404795 1212 70-71 -0.293999327112779 1212 72-73 -0.3697376104361396 1212 74-75 -0.3595596851404963 1212 76-77 -0.29293327371514266 1212 78-79 -0.28273610639946867 1212 80-81 -0.2676592704011398 1212 82-83 -0.23824955119570745 1212 84-85 -0.21284638136148715 1212 86-87 -0.22282011136893587 1212 88-89 -0.3568402599391547 1212 90-91 -0.3672628660387325 1212 92-93 -0.33658528086988326 1212 94-95 -0.4143266861522612 1212 96-97 -0.30969268097524605 1212 98-99 -0.16610947404108956 1212 100-101 -0.2490304340884002 1213 1 -1.2208885035254369 1213 2 -1.1127340026050518 1213 3 -1.3043757242351752 1213 4 -1.505203065227235 1213 5 -1.6760152598865936 1213 6 -1.9839570437884255 1213 7 -1.700632702492225 1213 8 -2.032962783477732 1213 9 -1.870236893044467 1213 10-11 -1.8526249811926263 1213 12-13 -2.14378227032109 1213 14-15 -2.5155365789842037 1213 16-17 -2.3595247297914312 1213 18-19 -2.182878482106453 1213 20-21 -2.1581106559328376 1213 22-23 -2.1335446824410624 1213 24-25 -1.9933501749984828 1213 26-27 -2.049240132944419 1213 28-29 -2.2821348168289575 1213 30-31 -2.2264933848188875 1213 32-33 -2.0015054323016273 1213 34-35 -2.287217145197303 1213 36-37 -2.4158713539127312 1213 38-39 -2.4307957172164407 1213 40-41 -2.5303356495805467 1213 42-43 -2.6507921182720793 1213 44-45 -2.6536385413630015 1213 46-47 -2.3014756077237095 1213 48-49 -2.655799714258581 1213 50-51 -2.566423539760663 1213 52-53 -2.583040843557736 1213 54-55 -2.5419587736265505 1213 56-57 -2.864449147650731 1213 58-59 -2.9359376361455993 1213 60-61 -2.8535830292677353 1213 62-63 -2.554774728552072 1213 64-65 -2.8186119319180634 1213 66-67 -2.5838318203035016 1213 68-69 -2.4336270060239187 1213 70-71 -2.3426830820687066 1213 72-73 -2.1571441012692816 1213 74-75 -2.2137100956361735 1213 76-77 -2.2166302312941966 1213 78-79 -2.1317242559508784 1213 80-81 -2.2720604742544417 1213 82-83 -2.0612068229887157 1213 84-85 -1.9425203429363478 1213 86-87 -1.905389266991289 1213 88-89 -2.214254895449322 1213 90-91 -2.145386394864083 1213 92-93 -2.2084910308028824 1213 94-95 -2.1698998169644135 1213 96-97 -2.1221643518173288 1213 98-99 -2.3471676000367587 1213 100-101 -2.2492777538147024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46193.0 3 2398.0 4 1187.0 5 319.0 6 464.0 7 1375.0 8 6950.0 9 20790.0 10 56439.0 11 81738.0 12 50818.0 13 36380.0 14 32077.0 15 31435.0 16 32373.0 17 34093.0 18 35832.0 19 38567.0 20 42815.0 21 48166.0 22 55009.0 23 62099.0 24 68792.0 25 76177.0 26 87459.0 27 105753.0 28 118898.0 29 121342.0 30 129406.0 31 144170.0 32 164613.0 33 190820.0 34 228668.0 35 289050.0 36 472886.0 37 1126583.0 38 485914.0 39 50.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.60121829334873 18.302095945315617 18.452888772057456 30.643796989278194 2 22.43026169511067 24.882902734150345 34.750321663413395 17.936513907325587 3 22.314227338704825 25.479745631346383 27.150877485008866 25.055149544939926 4 23.314240959363513 34.37425909402697 21.995903412103893 20.31559653450563 5 24.038148176504425 31.937624822512916 24.261374813196753 19.762852187785906 6 21.53653739522648 32.45721524240684 25.245623421799586 20.7606239405671 7 19.292297450020428 20.631001816620024 36.1143430101119 23.962357723247646 8 21.06600018221841 23.449079515838942 30.50882803193996 24.976092270002688 9 24.52108317006962 24.923208626409966 29.020739935899787 21.53496826762062 10-11 23.489229783054547 31.62048812029185 24.3253740693982 20.564908027255402 12-13 23.493062030059864 24.51464807860907 28.446464024499395 23.545825866831667 14-15 24.57059827704342 23.883236102680485 29.14554108062559 22.400624539650504 16-17 24.766490961466538 25.51390084757615 27.238900836405605 22.480707354551708 18-19 23.867956759457446 24.683079732319598 29.171557359814464 22.277406148408488 20-21 25.25331202610508 26.187552034151167 27.804748308366257 20.7543876313775 22-23 26.623848539089035 25.277125207814567 26.63104674159504 21.467979511501365 24-25 24.23122026819875 26.095841424579596 29.023928771914566 20.64900953530709 26-27 24.6301526039824 27.5846379820618 27.364301697791426 20.420907716164376 28-29 24.259307292052895 25.625840892346652 27.959854377494437 22.154997438106015 30-31 24.452195051711882 23.66699294848637 29.85523402618526 22.025577973616485 32-33 23.116348461772223 25.610455267443832 30.04904766785254 21.224148602931404 34-35 22.661791507866567 26.046754940259987 30.615241744026417 20.676211807847025 36-37 21.80343310309027 25.95489812507737 30.57633230149462 21.66533647033774 38-39 22.539587850130125 25.02882942849927 29.905130917187794 22.526451804182805 40-41 24.777249686790988 25.278540580699392 28.954886166729686 20.989323565779927 42-43 25.070540672885944 24.455224859639426 30.11259241247202 20.361642055002616 44-45 25.687056752446384 25.313241120188817 29.279293472030698 19.720408655334097 46-47 24.30540729288128 24.721522198958247 28.739858693428687 22.23321181473178 48-49 23.03904247828519 25.014988925454627 28.58726291552725 23.35870568073293 50-51 24.613432479189683 24.94709240879815 28.627941530814198 21.811533581197974 52-53 23.15365905913961 25.900625919179866 28.903773499336594 22.041941522343933 54-55 22.77719125490676 26.112996026338575 28.20198298840427 22.907829730350393 56-57 22.661037719959378 24.991388596133422 30.415657162923292 21.9319165209839 58-59 23.12294350738916 26.512437609229572 29.072750316177277 21.29186856720399 60-61 23.20031809731343 27.776150184821464 28.504948211456526 20.51858350640858 62-63 23.651801763666384 28.384889573719196 26.870547638135882 21.092761024478538 64-65 21.794795045449032 29.652776676414017 28.22674949120717 20.32567878692978 66-67 23.921188502788617 28.292098512361175 27.179800929647225 20.606912055202983 68-69 23.05530454640944 27.094290203651898 28.51290790103081 21.33749734890785 70-71 24.12386999647646 26.73150127902802 28.23067511794922 20.9139536065463 72-73 24.916178845264763 26.802434007312296 26.84105025472429 21.440336892698646 74-75 24.01002133486628 27.00892902008577 28.014200315478195 20.96684932956975 76-77 23.61691477237136 27.074442207406985 28.394001513880458 20.9146415063412 78-79 25.036986353068546 25.439866833496982 27.769800511522657 21.75334630191181 80-81 25.319123708013723 25.23485026279068 27.884059925699134 21.561966103496456 82-83 23.856730063595663 26.774598757606423 27.697434212414045 21.671236966383876 84-85 24.45009370820785 26.960084747144148 26.90827894654691 21.681542598101096 86-87 24.17888303394515 27.07569566132963 27.495552220294563 21.24986908443066 88-89 24.62278468178353 26.75585949037171 27.487784225635647 21.133571602209116 90-91 25.33894266928261 26.762453303149258 26.531762233868573 21.36684179369956 92-93 24.504159376617295 27.16979901465113 27.06527573306458 21.260765875666994 94-95 24.088762870039822 26.714741323675963 26.943163518392062 22.25333228789216 96-97 24.706509220772404 27.29408952342866 26.94542156171701 21.053979694081928 98-99 26.249842060829398 26.55591169527409 26.049746677300583 21.144499566595922 100-101 25.159818422943154 25.426675917577512 26.176893503916553 23.23661215556278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 38843.0 1 22529.0 2 5264.5 3 3336.0 4 1815.5 5 1097.5 6 835.5 7 677.0 8 608.5 9 648.5 10 703.0 11 766.0 12 883.5 13 973.0 14 1079.5 15 1310.0 16 1662.5 17 2043.0 18 2470.5 19 2950.0 20 3772.5 21 6996.0 22 11753.5 23 14193.0 24 12500.0 25 10229.5 26 8914.0 27 7506.0 28 7256.0 29 6794.5 30 6694.5 31 7624.5 32 8616.0 33 10210.0 34 15762.5 35 22194.0 36 33902.5 37 54045.0 38 76882.0 39 94424.5 40 115727.5 41 159017.5 42 209090.5 43 250394.0 44 303229.5 45 369886.5 46 420482.0 47 431434.5 48 393069.5 49 322186.5 50 245742.0 51 190277.5 52 154484.0 53 133678.0 54 110621.0 55 72152.5 56 43343.5 57 26298.0 58 15325.5 59 10407.5 60 7784.0 61 7139.5 62 8259.5 63 7619.5 64 5377.0 65 3521.5 66 2334.5 67 1803.5 68 1220.0 69 864.0 70 585.5 71 475.5 72 427.5 73 423.5 74 394.5 75 378.0 76 357.5 77 369.0 78 325.0 79 200.5 80 170.5 81 143.5 82 126.0 83 117.0 84 120.5 85 123.0 86 122.5 87 105.0 88 92.0 89 96.5 90 104.5 91 115.0 92 122.0 93 124.0 94 114.0 95 123.0 96 154.5 97 176.0 98 202.5 99 330.0 100 3742.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.797973895441309 2 2.5419061159895393 3 2.3516275486970466 4 2.3814413910652994 5 2.2153009939272517 6 2.3001048122191703 7 1.9430674866135849 8 1.8304153311169504 9 1.299574346668292 10-11 1.1223365748709504 12-13 1.117455938453629 14-15 1.1307286193894213 16-17 1.1492684124769383 18-19 1.1651912127343533 20-21 1.1897489851147214 22-23 1.2093156994393672 24-25 1.2351654049890262 26-27 1.2482945377948975 28-29 1.2758668209036113 30-31 1.3042451819726515 32-33 1.3114557149602328 34-35 1.2979732329114784 36-37 1.257779756533538 38-39 1.229467648447538 40-41 1.2068532969030263 42-43 1.1865688419287745 44-45 1.1711539812080038 46-47 1.162772978853373 48-49 1.154866789543866 50-51 1.1513332971150358 52-53 1.1475237505902036 54-55 1.1400371635066202 56-57 1.1368128516653129 58-59 1.1389881579418113 60-61 1.1369122311398738 62-63 1.1351565270892987 64-65 1.1334781181856046 66-67 1.1315678238412685 68-69 1.1300440052313354 70-71 1.1277472351525961 72-73 1.1198520880069291 74-75 1.114176415793121 76-77 1.1086663760369144 78-79 1.1063143951389744 80-81 1.1002191206992429 82-83 1.0981763203888255 84-85 1.1005724699421258 86-87 1.1046801548906406 88-89 1.1126857236747085 90-91 1.119266853323404 92-93 1.1286195660959635 94-95 1.137762477755561 96-97 1.1497763520135829 98-99 1.1590407274754213 100-101 1.1674438141577326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4528098.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.504692380009908 #Duplication Level Percentage of deduplicated Percentage of total 1 88.83026654942384 11.996254237842532 2 3.9490694821730075 1.0666193708806297 3 1.2971794546972373 0.5255402849206557 4 0.612786782177787 0.33101987951388606 5 0.5412253332228013 0.3654540816721144 6 0.22055776232377827 0.1787138839323579 7 0.22535679319483204 0.21303619174892022 8 0.16585635675431545 0.1791871261788967 9 0.15465246925723153 0.18796806208150663 >10 1.2006280416398774 3.5469902862569307 >50 0.6702032075772486 6.948765941750187 >100 2.0151501075765252 48.278731518559695 >500 0.0692894406255207 6.553710752023948 >1k 0.043134205947605836 10.940278566559783 >5k 0.0024878644381973263 2.4369859522200237 >10k+ 0.0021561489706417775 6.2507438638579345 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCAAATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTAT 61939 1.367881172183111 No Hit CTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATG 32624 0.720479106238425 No Hit GGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTT 27946 0.6171686213505096 No Hit CTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGT 27910 0.6163735855540229 No Hit TGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGG 18840 0.4160687334947256 No Hit GAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGC 17052 0.376581955602551 No Hit CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGGAAATC 15110 0.3336941912476276 No Hit TGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTT 14610 0.322652027407534 No Hit GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGG 14170 0.3129349232282517 No Hit AAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCT 14133 0.31211780310408477 No Hit CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGGAAATCTGGCCG 12273 0.27104095361893665 No Hit CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTGGGAAAT 11665 0.2576136823893829 No Hit CTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTT 10797 0.23844448596298048 No Hit GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTG 9570 0.2113470158993909 No Hit CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGGAAATCT 9252 0.20432419969709137 No Hit CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTGGGAAATCTGGCC 8385 0.18517708759836912 No Hit GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGG 8215 0.1814227518927373 No Hit ATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCA 8148 0.17994310193816476 No Hit CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC 7607 0.16799548066318354 No Hit CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGGAAATCTGGCCGC 7601 0.1678629746971024 No Hit GCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTT 6816 0.15052677746815551 No Hit CGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTT 6812 0.15043844015743474 No Hit AAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTT 6497 0.1434818769381758 No Hit GCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTT 6305 0.1392416860235799 No Hit GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTT 6180 0.1364811450635565 No Hit CGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTG 6162 0.13608362716531314 No Hit GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA 6077 0.13420645931249722 No Hit GAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTT 5174 0.11426431141728824 No Hit GGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGG 4857 0.10726357954266891 No Hit CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA 4799 0.10598268853721805 No Hit CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTTGGGAAA 4740 0.10467971320408702 No Hit CGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTT 4695 0.10368591845847858 No Hit CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT 4603 0.10165416031190139 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002009673818897029 0.0 0.0 2 0.0020317581465772163 0.0 0.0 3 0.002142179784978152 0.0 0.0 4 0.002296770078739462 0.0 0.0 5 0.002495529027861146 0.0 0.0 6 0.002517613355541333 0.0 0.0 7 0.002782625287703579 0.0 0.0 8 0.0029151312537847016 0.0 0.0 9 0.003290564824347883 0.0 0.0 10-11 0.003798504360992187 0.0 0.0 12-13 0.004218106586915743 0.0 0.0 14-15 0.0050241845472425726 0.0 0.0 16-17 0.005532124083886877 0.0 0.0 18-19 0.005830262507569403 0.0 0.0 20-21 0.006294033388853333 0.0 0.0 22-23 0.006735719942457075 0.0 0.0 24-25 0.007354081117502315 0.0 0.0 26-27 0.008115990422468772 0.0 0.0 28-29 0.009683977687762059 0.0 0.0 30-31 0.012378265664744889 0.0 0.0 32-33 0.014763373054205099 0.0 0.0 34-35 0.01814227518927373 0.0 0.0 36-37 0.022426634759230034 0.0 0.0 38-39 0.02703121708054905 0.0 0.0 40-41 0.03297190122651939 0.0 0.0 42-43 0.04080079538914573 0.0 0.0 44-45 0.049126586924576277 0.0 0.0 46-47 0.05937371496818311 0.0 0.0 48-49 0.07115570378556293 0.0 0.0 50-51 0.08522342051784215 0.0 0.0 52-53 0.10059411258325239 0.0 0.0 54-55 0.11949829707749257 0.0 0.0 56-57 0.13999255316470624 0.0 0.0 58-59 0.1619002062234519 0.0 0.0 60-61 0.18814742967135428 0.0 0.0 62-63 0.21706685676855933 0.0 0.0 64-65 0.25255637135062003 0.0 0.0 66-67 0.29225295035575644 0.0 0.0 68-69 0.3343456789141931 0.0 0.0 70-71 0.3839912475392538 0.0 0.0 72-73 0.4452090038687325 0.0 0.0 74-75 0.5135710402027518 0.0 0.0 76-77 0.5853671894910402 0.0 0.0 78-79 0.667730689574298 0.0 0.0 80-81 0.7636870933447112 0.0 0.0 82-83 0.8707960825936187 0.0 0.0 84-85 0.9851487313216278 0.0 0.0 86-87 1.1104441644151695 0.0 0.0 88-89 1.250491928399076 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAATC 450 0.0 70.7513 2 GAGCGGC 680 0.0 65.695045 9 GTCCAAT 525 0.0 60.664654 1 GATTGGG 740 0.0 59.993908 1 ATCCGGA 540 0.0 58.959423 2 TACGTAG 25 0.004751773 57.80271 3 GATCCGG 585 0.0 56.092415 1 GATCCGA 285 0.0 54.182148 1 ACGGGAG 905 0.0 51.096317 3 GGGAGAG 4395 0.0 46.721546 5 CTTGTTA 565 0.0 45.266663 1 CAATCAA 800 0.0 43.92673 4 TCGGGGG 4710 0.0 42.38117 38-39 CAACTAG 14605 0.0 42.346386 8 AACTAGC 14790 0.0 41.70808 9 GATCAGG 245 0.0 41.362263 1 TCCGGAA 495 0.0 40.8706 3 GGATCAT 920 0.0 40.474552 50-51 TGGGAGA 780 0.0 40.115738 4 GATCATT 1170 0.0 39.529285 50-51 >>END_MODULE