FastQCFastQC Report
Thu 14 Aug 2014
lane1_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528098
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTAT619391.367881172183111No Hit
CTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATG326240.720479106238425No Hit
GGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTT279460.6171686213505096No Hit
CTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGT279100.6163735855540229No Hit
TGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGG188400.4160687334947256No Hit
GAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGC170520.376581955602551No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGGAAATC151100.3336941912476276No Hit
TGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTT146100.322652027407534No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGG141700.3129349232282517No Hit
AAAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCT141330.31211780310408477No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGGAAATCTGGCCG122730.27104095361893665No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTGGGAAAT116650.2576136823893829No Hit
CTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTT107970.23844448596298048No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTG95700.2113470158993909No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGGAAATCT92520.20432419969709137No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTGGGAAATCTGGCC83850.18517708759836912No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGG82150.1814227518927373No Hit
ATCAACTAGCCCTGAAAATGGATGGCGCTTGAGCGGGAGACTTATACTCA81480.17994310193816476No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC76070.16799548066318354No Hit
CTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTGGGAAATCTGGCCGC76010.1678629746971024No Hit
GCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTT68160.15052677746815551No Hit
CGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTT68120.15043844015743474No Hit
AAATGGATGGCGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTT64970.1434818769381758No Hit
GCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTT63050.1392416860235799No Hit
GGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTT61800.1364811450635565No Hit
CGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTG61620.13608362716531314No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA60770.13420645931249722No Hit
GAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTT51740.11426431141728824No Hit
GGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTGGG48570.10726357954266891No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA47990.10598268853721805No Hit
CTTATACTCAACCGTTGCATGGCTTGTGTTTTTTTTTTTTTTTTGGGAAA47400.10467971320408702No Hit
CGCTTGAGCGGGAGACTTATACTCAACCGTTGCATGGCTTGTGTTTTTTT46950.10368591845847858No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT46030.10165416031190139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAATC4500.070.75132
GAGCGGC6800.065.6950459
GTCCAAT5250.060.6646541
GATTGGG7400.059.9939081
ATCCGGA5400.058.9594232
TACGTAG250.00475177357.802713
GATCCGG5850.056.0924151
GATCCGA2850.054.1821481
ACGGGAG9050.051.0963173
GGGAGAG43950.046.7215465
CTTGTTA5650.045.2666631
CAATCAA8000.043.926734
TCGGGGG47100.042.3811738-39
CAACTAG146050.042.3463868
AACTAGC147900.041.708089
GATCAGG2450.041.3622631
TCCGGAA4950.040.87063
GGATCAT9200.040.47455250-51
TGGGAGA7800.040.1157384
GATCATT11700.039.52928550-51
Produced by FastQC (version 0.11.2)