##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	lane1_Undetermined_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4528098
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51552196971002	33.0	33.0	33.0	33.0	33.0
2	32.479368379394614	33.0	33.0	33.0	33.0	33.0
3	32.46238663562494	33.0	33.0	33.0	33.0	33.0
4	36.27417803236591	37.0	37.0	37.0	33.0	37.0
5	36.086928330614754	37.0	37.0	37.0	33.0	37.0
6	36.01662464019109	37.0	37.0	37.0	33.0	37.0
7	35.846583929941445	37.0	37.0	37.0	33.0	37.0
8	35.83710379059817	37.0	37.0	37.0	33.0	37.0
9	35.808466380365445	37.0	37.0	37.0	33.0	37.0
10-11	35.7881132431321	37.0	37.0	37.0	33.0	37.0
12-13	35.85104209317025	37.0	37.0	37.0	33.0	37.0
14-15	37.53313974211689	40.0	37.0	40.0	33.0	40.0
16-17	37.566324977948796	40.0	37.0	40.0	33.0	40.0
18-19	37.682376684426885	40.0	37.0	40.0	33.0	40.0
20-21	37.797413947312975	40.0	37.0	40.0	33.0	40.0
22-23	37.77127283905958	40.0	37.0	40.0	33.0	40.0
24-25	37.74820255657011	40.0	37.0	40.0	33.0	40.0
26-27	37.63154673330833	40.0	37.0	40.0	33.0	40.0
28-29	37.53024790982881	40.0	37.0	40.0	33.0	40.0
30-31	37.19495161102962	40.0	37.0	40.0	33.0	40.0
32-33	37.02879553401892	40.0	37.0	40.0	33.0	40.0
34-35	36.77563581883608	40.0	37.0	40.0	33.0	40.0
36-37	36.45937775640015	40.0	37.0	40.0	33.0	40.0
38-39	36.14727198925465	40.0	37.0	40.0	30.0	40.0
40-41	36.10726037731516	40.0	37.0	40.0	27.0	40.0
42-43	35.93391264941704	37.0	37.0	40.0	27.0	40.0
44-45	35.91167516692439	37.0	37.0	40.0	27.0	40.0
46-47	35.70310691155536	37.0	37.0	40.0	27.0	40.0
48-49	35.235809936092366	37.0	37.0	40.0	27.0	40.0
50-51	34.77379862361636	37.0	33.0	40.0	24.5	40.0
52-53	34.56363046912854	37.0	33.0	40.0	22.0	40.0
54-55	34.594497181818944	37.0	33.0	40.0	27.0	40.0
56-57	34.4765086798033	37.0	33.0	40.0	27.0	40.0
58-59	33.831715325065844	37.0	33.0	38.5	22.0	40.0
60-61	33.8722461395491	37.0	33.0	37.0	24.5	40.0
62-63	33.98477131016158	37.0	33.0	37.0	27.0	40.0
64-65	33.71252157086706	37.0	33.0	37.0	27.0	40.0
66-67	33.73114285512372	37.0	33.0	37.0	27.0	40.0
68-69	33.266907540428676	37.0	33.0	37.0	24.5	37.0
70-71	33.21074643260813	37.0	33.0	37.0	27.0	37.0
72-73	32.88951884433597	37.0	33.0	37.0	22.0	37.0
74-75	32.33565561081054	37.0	33.0	37.0	22.0	37.0
76-77	31.999456946382345	37.0	33.0	37.0	18.5	37.0
78-79	31.91495226030885	37.0	33.0	37.0	18.5	37.0
80-81	31.745087341307542	37.0	33.0	37.0	15.0	37.0
82-83	31.819119749616725	37.0	33.0	37.0	15.0	37.0
84-85	31.73512697825886	37.0	33.0	37.0	15.0	37.0
86-87	31.624955996977096	37.0	33.0	37.0	15.0	37.0
88-89	31.615008994946663	37.0	33.0	37.0	15.0	37.0
90-91	31.579278982036165	33.0	33.0	37.0	15.0	37.0
92-93	31.459462781061717	33.0	33.0	37.0	15.0	37.0
94-95	31.341285568466056	33.0	33.0	37.0	15.0	37.0
96-97	31.29719906680465	35.0	33.0	37.0	15.0	37.0
98-99	31.102299353945078	33.0	33.0	37.0	15.0	37.0
100-101	30.750306861733115	33.0	30.0	37.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.10058399526811002
2201	2	0.11788847421689042
2201	3	0.15071055451097948
2201	4	0.1447773488631583
2201	5	0.19466778825844955
2201	6	0.2708022183729568
2201	7	0.27573662845092173
2201	8	0.30095100737071334
2201	9	0.2530081886911404
2201	10-11	0.27190938682606713
2201	12-13	0.2964501609401893
2201	14-15	0.4743855856619703
2201	16-17	0.5076870106961238
2201	18-19	0.5048503406607239
2201	20-21	0.5129634114319757
2201	22-23	0.32924628132553124
2201	24-25	-0.044577446685607924
2201	26-27	-0.010167162910597938
2201	28-29	0.05526010495589162
2201	30-31	-0.327926413334545
2201	32-33	-0.1037742303707887
2201	34-35	0.10634548256007292
2201	36-37	0.0391310863550558
2201	38-39	0.23316731809202906
2201	40-41	0.28589753308327914
2201	42-43	0.3995868186875029
2201	44-45	0.13946605645751475
2201	46-47	0.20973520723942585
2201	48-49	0.03823178545994921
2201	50-51	0.37695535463966934
2201	52-53	0.6070689906453381
2201	54-55	0.4000652045504438
2201	56-57	0.1684819224630445
2201	58-59	0.5382070141884299
2201	60-61	0.7814000916102088
2201	62-63	0.7795442451607997
2201	64-65	0.8477433628132829
2201	66-67	0.7205149752613167
2201	68-69	0.46369014954717613
2201	70-71	0.5335718564409788
2201	72-73	0.43857340278291446
2201	74-75	0.447737780115375
2201	76-77	0.4206949076665225
2201	78-79	0.334480762276403
2201	80-81	0.13971983112717723
2201	82-83	0.17566924097102543
2201	84-85	0.15930763152190153
2201	86-87	0.4121190451115453
2201	88-89	0.3514428743319833
2201	90-91	0.3370547272224975
2201	92-93	0.48323259299720434
2201	94-95	0.4681343652763239
2201	96-97	0.40586320347482996
2201	98-99	0.4071056774171993
2201	100-101	0.5943750115279833
1101	1	0.18067264719631737
1101	2	0.19489377939057562
1101	3	0.17280911487866746
1101	4	0.229931275821464
1101	5	0.27714124436156595
1101	6	0.3769790078174182
1101	7	0.3210007934088921
1101	8	0.32293731055165154
1101	9	0.3606972050345405
1101	10-11	0.34738215304026454
1101	12-13	0.325462273658907
1101	14-15	0.33708711375391687
1101	16-17	0.325109710685787
1101	18-19	0.4527420930026196
1101	20-21	0.356922917115746
1101	22-23	0.4689340044276733
1101	24-25	0.6061241006366842
1101	26-27	0.6994533020312659
1101	28-29	0.5405308563098288
1101	30-31	0.49732267983411305
1101	32-33	0.6147116677708411
1101	34-35	0.8637640720617412
1101	36-37	0.5799471504305913
1101	38-39	0.7135821648862049
1101	40-41	0.8258106118868582
1101	42-43	0.8625465142710098
1101	44-45	0.4982498808459255
1101	46-47	0.7368447168894576
1101	48-49	0.5468177775899292
1101	50-51	0.8781883808482434
1101	52-53	0.7350856922070861
1101	54-55	0.7542585032531193
1101	56-57	0.6433382356678123
1101	58-59	1.0471309797757087
1101	60-61	1.3856944698568299
1101	62-63	1.2872242472761357
1101	64-65	1.293561121563691
1101	66-67	1.1679930346302214
1101	68-69	0.8360680307949409
1101	70-71	0.8532191477255395
1101	72-73	0.7424913468658545
1101	74-75	0.8946730627655128
1101	76-77	0.9599493468733655
1101	78-79	0.8113108308566446
1101	80-81	0.9024596997357897
1101	82-83	0.8803660143581382
1101	84-85	0.6933260061243764
1101	86-87	0.8155351971659499
1101	88-89	0.8098157701106921
1101	90-91	0.6471477780939843
1101	92-93	0.7951587772864883
1101	94-95	0.9204695311020643
1101	96-97	0.585505350639913
1101	98-99	0.8222390038596536
1101	100-101	0.8376244364202314
2202	1	0.09099704986783053
2202	2	0.09482089452337306
2202	3	0.09423979541244876
2202	4	0.15749102285447236
2202	5	0.14204924938005092
2202	6	0.2121348804866301
2202	7	0.19842449893417324
2202	8	0.1883191499500967
2202	9	0.208370291227574
2202	10-11	0.18294786419727416
2202	12-13	0.27899775709975927
2202	14-15	0.27699027829995515
2202	16-17	0.24977470152849435
2202	18-19	0.25138108690303795
2202	20-21	0.3001573669059141
2202	22-23	0.2492362130306347
2202	24-25	0.29880476255326727
2202	26-27	0.40271410195864377
2202	28-29	0.26548926607219414
2202	30-31	0.010729464628006724
2202	32-33	0.02020494796575889
2202	34-35	0.3654428944805872
2202	36-37	0.18721928947496025
2202	38-39	0.28143969021919446
2202	40-41	0.47106030579642066
2202	42-43	0.4553844590359617
2202	44-45	0.1349546337761538
2202	46-47	0.18871091701101506
2202	48-49	0.03604500177815595
2202	50-51	0.3661014315421127
2202	52-53	0.46049566663000263
2202	54-55	0.26137124358937314
2202	56-57	0.2276265703774314
2202	58-59	0.7074663991024721
2202	60-61	0.9462291582545248
2202	62-63	0.8781454713352801
2202	64-65	0.9558688176903019
2202	66-67	0.8781762775410797
2202	68-69	0.3839485185254361
2202	70-71	0.48842322417166883
2202	72-73	0.27702917571951247
2202	74-75	0.5186231176005549
2202	76-77	0.5133212826297111
2202	78-79	0.30518010949504415
2202	80-81	0.37153863170652457
2202	82-83	0.3740731874063883
2202	84-85	0.09390605902796167
2202	86-87	0.28880996176769
2202	88-89	0.343975722961293
2202	90-91	0.25772468912315105
2202	92-93	0.35163934837476774
2202	94-95	0.4432384397368736
2202	96-97	0.2725462770413287
2202	98-99	0.19217632476513202
2202	100-101	0.2883846329138642
1102	1	0.09807176910076265
1102	2	0.09869506629835456
1102	3	0.09631248390067526
1102	4	0.14304223799024385
1102	5	0.17252670163689032
1102	6	0.22304982338400947
1102	7	0.2008521908546257
1102	8	0.13101733014153183
1102	9	0.14996002094142824
1102	10-11	0.12265970504137869
1102	12-13	0.18033841712026089
1102	14-15	0.253447017965847
1102	16-17	0.24713901042815678
1102	18-19	0.24572928485581258
1102	20-21	0.2623504282471387
1102	22-23	0.3211401648922205
1102	24-25	0.2846983622512198
1102	26-27	0.31466368052746674
1102	28-29	0.2196434371726781
1102	30-31	0.10743233256214779
1102	32-33	0.24863750674987273
1102	34-35	0.4185448528287097
1102	36-37	0.2582106066873351
1102	38-39	0.40051172755139675
1102	40-41	0.4683889915143098
1102	42-43	0.5452691393145557
1102	44-45	0.170403284299681
1102	46-47	0.3118119808710915
1102	48-49	0.15415260009115883
1102	50-51	0.4450299845050196
1102	52-53	0.6666891420488383
1102	54-55	0.5292070280916761
1102	56-57	0.2894573361429522
1102	58-59	0.6883778455678069
1102	60-61	0.8597982962059092
1102	62-63	0.8528677528984545
1102	64-65	0.8412048755083958
1102	66-67	0.7584596392929299
1102	68-69	0.5005260844302697
1102	70-71	0.5654168353382758
1102	72-73	0.38897200933480036
1102	74-75	0.5618363933665336
1102	76-77	0.6479274393205685
1102	78-79	0.46015906334158885
1102	80-81	0.4945734520824949
1102	82-83	0.5335978267551766
1102	84-85	0.27295069442804376
1102	86-87	0.43944536654250754
1102	88-89	0.4699161107492209
1102	90-91	0.39257157588599156
1102	92-93	0.4902668262657812
1102	94-95	0.4782335144690606
1102	96-97	0.2537964787478231
1102	98-99	0.16039166924408832
1102	100-101	0.26283484940565316
2203	1	0.030346691035610718
2203	2	0.052706360030775556
2203	3	0.03992991694812531
2203	4	-0.011204844376678125
2203	5	0.05278362699245065
2203	6	0.11735533325929026
2203	7	0.07434442454241719
2203	8	0.0622743791011402
2203	9	0.0661843148265362
2203	10-11	0.07660190453490401
2203	12-13	0.09467093811691285
2203	14-15	0.11017608258254796
2203	16-17	0.14885219898079072
2203	18-19	0.15642385838118855
2203	20-21	0.1464429285604254
2203	22-23	0.18331781599604113
2203	24-25	0.2023124960541054
2203	26-27	0.24569070405201643
2203	28-29	0.16870435643613746
2203	30-31	0.08058822635846496
2203	32-33	0.11700922862278418
2203	34-35	0.3458108594468783
2203	36-37	0.1125157166671471
2203	38-39	0.22647008667121327
2203	40-41	0.2981493835572806
2203	42-43	0.32789535979767237
2203	44-45	0.14524207510131504
2203	46-47	0.15652074362872526
2203	48-49	-0.026378553049134723
2203	50-51	0.2796137147043183
2203	52-53	0.4429185319281004
2203	54-55	0.21521977513405943
2203	56-57	-6.294475897306029E-4
2203	58-59	0.45059374335880165
2203	60-61	0.6421084495733069
2203	62-63	0.5583113246535945
2203	64-65	0.5632611422981313
2203	66-67	0.44415758092187474
2203	68-69	0.25827123639269445
2203	70-71	0.2790330195143085
2203	72-73	0.36148069262656435
2203	74-75	0.41575545452316476
2203	76-77	0.5118810219349115
2203	78-79	0.4115243321419122
2203	80-81	0.44446745839784896
2203	82-83	0.2595187411172404
2203	84-85	0.18699874432815378
2203	86-87	0.24654336146234357
2203	88-89	0.2115135263552368
2203	90-91	0.1107198909146625
2203	92-93	0.2544607738585185
2203	94-95	0.23038715473678195
2203	96-97	0.08490688154734727
2203	98-99	0.05609751896636439
2203	100-101	0.10744272944214828
1103	1	0.031246885077479192
1103	2	0.020178937327564483
1103	3	0.059441367986629245
1103	4	0.06156898724286464
1103	5	0.052408349859746295
1103	6	0.09325515836063403
1103	7	-0.027324162143557373
1103	8	0.0881581834290941
1103	9	0.03852573981075125
1103	10-11	0.022164229512235067
1103	12-13	0.06087380763211314
1103	14-15	0.11512899451548009
1103	16-17	0.1308427215031287
1103	18-19	0.07136293150086459
1103	20-21	0.1386312694894869
1103	22-23	0.10129393666435504
1103	24-25	0.12077855723586595
1103	26-27	0.206871980332771
1103	28-29	0.06336997042028969
1103	30-31	-0.04421051345741489
1103	32-33	0.15502845613552552
1103	34-35	0.2276579057923911
1103	36-37	-0.08885822410876898
1103	38-39	-0.03574208448083738
1103	40-41	0.2577047889612274
1103	42-43	0.34103157063922396
1103	44-45	0.11697497750099473
1103	46-47	0.16166633839961975
1103	48-49	0.07817476294246717
1103	50-51	0.5250947351946493
1103	52-53	0.36511111129596685
1103	54-55	0.2355045630366135
1103	56-57	-0.11614751971413995
1103	58-59	0.49840861283765037
1103	60-61	0.7449071302753296
1103	62-63	0.5790409568644961
1103	64-65	0.7061278377074132
1103	66-67	0.49158507326938405
1103	68-69	0.2655517982795246
1103	70-71	0.3063777866411286
1103	72-73	0.27756258508386367
1103	74-75	0.3903864994052597
1103	76-77	0.4104243467669093
1103	78-79	0.2739822197693833
1103	80-81	0.27216117447772703
1103	82-83	0.23984271550634162
1103	84-85	-0.01206989136646186
1103	86-87	0.2566578347449209
1103	88-89	0.30506539018024625
1103	90-91	0.10302938816086638
1103	92-93	0.13481199565197244
1103	94-95	0.2812922763233239
1103	96-97	0.18157268679694383
1103	98-99	0.2053437588787581
1103	100-101	0.22204296299609183
2204	1	0.0334807595208062
2204	2	0.08686013630572376
2204	3	0.058759057224186506
2204	4	0.055442139734282136
2204	5	0.07927197306897682
2204	6	0.08501739891827498
2204	7	0.04659033872983542
2204	8	0.10005933456304916
2204	9	0.039063435335926044
2204	10-11	0.06978080550809551
2204	12-13	0.10297781775893355
2204	14-15	0.05854158932424269
2204	16-17	0.15640331895185966
2204	18-19	0.16940774223728283
2204	20-21	0.19655946779516142
2204	22-23	0.18435804747635132
2204	24-25	0.22864947991272544
2204	26-27	0.27848583724571796
2204	28-29	0.2595353556202298
2204	30-31	0.19176173565721655
2204	32-33	0.2539924563936893
2204	34-35	0.4775107088837345
2204	36-37	0.20586351752005783
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1115	10-11	-0.4342124814405892
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1115	14-15	-0.6788788766750358
1115	16-17	-0.738948284449684
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1115	24-25	-0.7099170487743152
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1213	56-57	-1.037724396800897
1213	58-59	-0.8035344320995179
1213	60-61	-0.7725079272406248
1213	62-63	-1.207342976081911
1213	64-65	-1.1122963500587417
1213	66-67	-1.2809005484000835
1213	68-69	-1.1325524312216615
1213	70-71	-1.4562286489003853
1213	72-73	-1.7386229071136086
1213	74-75	-1.5229948682068226
1213	76-77	-1.4359366103631501
1213	78-79	-1.5516382579380554
1213	80-81	-1.2259981055915823
1213	82-83	-1.1790248599699886
1213	84-85	-1.0090080731248356
1213	86-87	-1.3009525951214869
1213	88-89	-1.3535702666376324
1213	90-91	-1.4623462169297952
1213	92-93	-1.4775541777555006
1213	94-95	-1.5509460970860545
1213	96-97	-1.6222457252159792
1213	98-99	-1.5673705564767566
1213	100-101	-1.6086909865050991
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	26.0
9	294.0
10	2118.0
11	4433.0
12	5708.0
13	5576.0
14	5557.0
15	7896.0
16	9513.0
17	13011.0
18	17217.0
19	23508.0
20	31048.0
21	39724.0
22	45037.0
23	48360.0
24	54911.0
25	62449.0
26	71574.0
27	87897.0
28	106849.0
29	107052.0
30	107566.0
31	121293.0
32	148744.0
33	192677.0
34	255785.0
35	329293.0
36	516939.0
37	1363338.0
38	742608.0
39	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.46141493198407	17.920563292049373	18.327976336291147	35.29004543967541
2	19.823836320931623	24.943953542511597	34.70593224419513	20.526277892361648
3	19.974080401992293	24.844738604299437	28.54203586339471	26.639145130313562
4	22.35269643015677	29.554263180699714	21.802354984366502	26.290685404777015
5	25.905628694057548	31.099158051386226	22.538960635959317	20.456252618596903
6	20.72441895029657	32.6704943223402	24.668414862045832	21.936671865317404
7	16.1541115055372	22.989475934487285	38.0077462987771	22.84866626119841
8	19.105637731338852	25.37842599696385	27.300469203625894	28.21546706807141
9	24.954760254747136	22.766115044329872	27.901670856063625	24.377453844859364
10-11	23.283628137023534	32.39841540532029	22.231873073418466	22.08608338423771
12-13	20.256312177936866	26.599441744351243	27.03686125962421	26.107384818087674
14-15	21.386204805267344	28.155274620938144	26.928073380960342	23.530447192834174
16-17	23.272967908076893	26.912577571110486	25.865302686112674	23.94915183469994
18-19	21.851178050001906	25.99786546935657	26.859076255085796	25.29188022555573
20-21	22.94520789965235	27.823669010697206	26.382357448977473	22.848765640672973
22-23	22.054663335909915	29.373603199826604	24.944448683455363	23.627284780808118
24-25	21.463207842346975	25.85503881929934	27.275518474521743	25.40623486383194
26-27	20.905420826063928	28.714011193592338	24.5508381920167	25.82972978832704
28-29	22.77168247324681	28.29087557738224	25.053996277461078	23.88344567190987
30-31	21.90556861091428	26.10185448368169	26.427782515071492	25.56479439033254
32-33	21.875630281235864	28.065090639928464	24.932639430849722	25.12663964798595
34-35	20.515655196757475	27.939706929509	26.312323667709425	25.232314206024093
36-37	21.706423851036913	29.306315662768395	25.980083158874738	23.007177327319955
38-39	23.185455377137718	26.413004177019307	25.789215452430174	24.612324993412802
40-41	20.246296231604486	28.29499252276347	27.116056822756512	24.342654422875533
42-43	20.548440640714364	28.33609234456252	26.926256541930844	24.189210472792272
44-45	22.76684286830132	27.93372825048789	25.53398877058556	23.76544011062523
46-47	21.086647714610198	27.736314582742754	27.428911959407298	23.74812574323975
48-49	21.724080049028004	26.941517885306922	26.336688987625838	24.99771307803924
50-51	20.684360005246045	27.156825058000443	25.12558848402896	27.033226452724552
52-53	22.619611414829787	28.329284167003287	25.842630240195618	23.208474177971308
54-55	21.483967120215926	25.730304905460322	27.16297460136083	25.622753372962926
56-57	20.811954387814257	25.75398856705242	28.15993719867346	25.274119846459858
58-59	22.14836441831136	28.806756662304757	25.939558549162182	23.105320370221698
60-61	21.205409174481275	25.465902267254283	26.514263267576748	26.814425290687694
62-63	22.167313165495116	27.805453066692397	27.405509571443698	22.62172419636879
64-65	21.518746092376418	29.650365467742894	26.10437991888877	22.72650852099192
66-67	21.871298327598467	29.850041277231337	23.845668362666856	24.432992032503343
68-69	20.824940471730084	30.31396478968542	25.457173757679396	23.4039209809051
70-71	21.069092465039773	28.288561830907256	25.593991814597576	25.048353889455388
72-73	21.34717895219019	28.7466827339236	25.701163711295965	24.204974602590244
74-75	21.511828254428842	28.33097498055549	26.658506224763435	23.498690540252237
76-77	21.820015184188243	27.222410200901514	25.465903753793945	25.4916708611163
78-79	22.386178904551905	26.95773448059987	25.090912061675386	25.56517455317284
80-81	23.45993260771709	26.976940604943678	25.273965701570166	24.289161085769067
82-83	22.159697590651884	27.332051119862154	26.31269592034672	24.195555369139246
84-85	22.902316872294488	28.375873602866175	24.349629923984388	24.372179600854952
86-87	21.79628228904446	28.316138752853952	24.809227120282053	25.078351837819536
88-89	21.43246146549386	29.205305781773227	25.324354465959587	24.037878286773328
90-91	22.78215421531923	29.006276225721578	24.663440312142175	23.54812924681702
92-93	21.16099260998261	27.790589066239228	27.115517818368602	23.93290050540956
94-95	20.88109729020638	28.54335935665948	25.231135445557772	25.344407907576365
96-97	20.500502603911222	29.836493993500795	24.71428744984085	24.948715952747133
98-99	21.206306880629118	30.506369088858015	25.046667506991625	23.24065652352124
100-101	20.9841981202675	30.53734808676596	24.43993230820339	24.038521484763155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1565.0
1	1007.5
2	360.0
3	248.0
4	218.0
5	227.5
6	253.0
7	296.0
8	382.0
9	529.5
10	713.5
11	989.0
12	1352.0
13	1806.0
14	2314.0
15	2752.5
16	3097.0
17	3304.5
18	3453.5
19	3509.5
20	3572.5
21	3924.0
22	4407.0
23	4822.5
24	5507.0
25	6740.0
26	8607.0
27	10222.5
28	22073.5
29	24133.5
30	13552.0
31	13892.5
32	15033.5
33	18195.0
34	22778.5
35	24229.5
36	35697.0
37	57336.5
38	80258.5
39	95171.0
40	115072.0
41	160406.5
42	215326.5
43	232262.5
44	244300.5
45	290113.5
46	336721.0
47	386493.5
48	399892.0
49	360306.0
50	292091.5
51	219867.5
52	171943.0
53	192188.0
54	174671.5
55	90777.5
56	48929.0
57	26429.0
58	14250.0
59	9257.5
60	6747.5
61	6413.0
62	8440.5
63	8097.5
64	5268.0
65	3101.0
66	2054.0
67	1506.5
68	758.0
69	563.5
70	430.5
71	375.5
72	342.5
73	385.5
74	425.5
75	408.5
76	332.5
77	337.5
78	320.0
79	179.0
80	170.0
81	155.5
82	170.5
83	197.5
84	195.5
85	203.5
86	195.0
87	206.5
88	203.5
89	157.0
90	135.5
91	133.5
92	83.5
93	41.0
94	29.0
95	21.0
96	18.5
97	11.5
98	9.5
99	24.0
100	110.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02201807469714657
2	0.01333893391883303
3	0.005521081920046783
4	0.0
5	0.008546634812232421
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0014354812992121637
14-15	0.006073190112051462
16-17	0.03191185349787041
18-19	0.0037653778694719062
20-21	0.0
22-23	0.14582281567227565
24-25	0.05551999978799045
26-27	0.1769396333736593
28-29	0.1484398085023778
30-31	0.7036066798907621
32-33	0.6320203317154355
34-35	0.4281599028996281
36-37	0.8342906889382695
38-39	0.9302802192002028
40-41	1.0637468535354138
42-43	0.8650651995606101
44-45	0.886740967178714
46-47	0.9051482543001499
48-49	1.983570143579048
50-51	2.334710953694023
52-53	1.699963207510085
54-55	1.3560218882188504
56-57	1.0305430668682523
58-59	2.2083113042164726
60-61	1.895508886954302
62-63	1.3519362875980159
64-65	1.679811258501914
66-67	0.8332195990457804
68-69	1.2705776244242064
70-71	0.645646361894111
72-73	0.779952200681169
74-75	1.4017033200253175
76-77	1.6078605189198643
78-79	1.3321045613412077
80-81	2.0248015833579576
82-83	2.066629299984232
84-85	1.9657260068134568
86-87	2.102626754102937
88-89	2.0083156327446976
90-91	1.5600810759837795
92-93	2.101511495555087
94-95	2.399804509531375
96-97	1.838431942064858
98-99	2.5862072773159945
100-101	1.87758745504183
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4528098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.732204700255663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53418883219979	7.119732269520676
2	6.173156243397941	1.0781052792802415
3	2.2861982835502475	0.5989065419200169
4	1.4233335705141201	0.4971536037790029
5	0.886862939066568	0.38721343624998183
6	0.5135256164831947	0.26905264811737434
7	0.42781310057826366	0.26150260973903233
8	0.3294197572572587	0.23012486021431305
9	0.26289095337759266	0.2066055856864656
>10	1.9228631167733161	3.4723749172402485
>50	0.9339428599466617	6.400200573024625
>100	3.0927781324461283	48.250143142349586
>500	0.12031105534949404	7.405145758052085
>1k	0.08419387871436398	14.253279581935294
>5k	0.004762104637983967	2.8270330495652503
>10k+	0.0037595557070969495	6.743426143325612
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT	68338	1.5091987850086284	No Hit
CACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAAC	32355	0.7145384220924547	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	27192	0.6005170382796485	TruSeq Adapter, Index 2 (100% over 50bp)
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA	23982	0.5296263464262478	No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA	22653	0.5002762749392792	No Hit
GGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAACCATTGCG	17552	0.3876241194426446	No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA	14977	0.33075697566616274	No Hit
CTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCT	14664	0.32384458110226416	No Hit
CCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTAC	14017	0.30955602109318303	No Hit
CTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCTT	13393	0.2957754006207463	No Hit
CAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTC	12464	0.27525906020585245	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	12344	0.27260894088423	TruSeq Adapter, Index 3 (100% over 50bp)
AAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCA	11849	0.26167719868253736	No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG	11380	0.2513196490005296	No Hit
CTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACT	10954	0.24191172540876985	No Hit
CTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAAC	9827	0.21702268811319897	No Hit
ATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACG	9553	0.21097158232882768	No Hit
CTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAACCATTGCGACCCT	9447	0.20863064359472788	No Hit
CTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCAAGATC	9167	0.20244703184427543	No Hit
GTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGC	9120	0.20140906844330667	No Hit
ACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAG	7508	0.16580913222284502	No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA	6671	0.14732454995452837	No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA	6318	0.1395287822834223	No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC	6258	0.13820372262261107	No Hit
TACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAA	6115	0.13504566376434432	No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC	6073	0.13411812200177647	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	5810	0.12830994382188723	No Hit
CTTCAACGCTAACCATTGCGACCCTCTTACTCGTTGCGGCCAGATTTCCC	5645	0.12466602975465636	No Hit
CAAGGATCTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATG	5451	0.12038167018470006	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	5380	0.11881368291940678	No Hit
CACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTC	5368	0.11854867098724453	No Hit
TTAACGTTCACATCGAGATGTAACTTTATTGCATACAATATTATAATATT	5209	0.11503726288609478	No Hit
GGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTA	5169	0.11415388977888728	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	5081	0.11221046894303083	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	4947	0.10925116903388575	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC	4809	0.10620353181401992	TruSeq Adapter, Index 2 (98% over 50bp)
TTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCA	4769	0.10532015870681245	No Hit
TCTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCT	4706	0.10392884606296064	No Hit
CCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAA	4691	0.10359758114775784	No Hit
CCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCAAG	4667	0.10306755728343335	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	4654	0.10278046102359091	No Hit
TTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCAC	4548	0.10043952228949107	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0020538424742574035	0.0	0.0
2	0.0020980111296177778	0.0	0.0
3	0.0022305170956989006	0.0	0.0
4	0.002429276044820585	0.0	0.0
5	0.00269428797698283	0.0	0.0
6	0.0027163723046630174	0.0	0.0
7	0.0030255528921856374	0.0	0.0
8	0.0031580588582667602	0.0	0.0
9	0.0037543357056318127	0.0	0.0
10-11	0.004306443897636491	0.0	0.0
12-13	0.0047370882874001406	0.0	0.0
14-15	0.005443786773166128	0.0	0.0
16-17	0.0060842322758915554	0.0	0.0
18-19	0.006503834501815111	0.0	0.0
20-21	0.007055942693819789	0.0	0.0
22-23	0.00758596655814428	0.0	0.0
24-25	0.008160159077829146	0.0	0.0
26-27	0.008899984055115415	0.0	0.0
28-29	0.010788194071771414	0.0	0.0
30-31	0.013537692867954712	0.0	0.0
32-33	0.01587863160205455	0.0	0.0
34-35	0.019246491573283085	0.0	0.0
36-37	0.02353085114323939	0.0	0.0
38-39	0.028091264809198033	0.0	0.0
40-41	0.033954653808287716	0.0	0.0
42-43	0.04172833715171359	0.0	0.0
44-45	0.049811201082662085	0.0	0.0
46-47	0.05966081122802554	0.0	0.0
48-49	0.07117778811324313	0.0	0.0
50-51	0.085322799992403	0.0	0.0
52-53	0.10036222714261042	1.1042163840093567E-5	0.0
54-55	0.11843824934884359	2.2084327680187133E-5	0.0
56-57	0.13842456589941296	2.2084327680187133E-5	0.0
58-59	0.15920591824646907	2.2084327680187133E-5	0.0
60-61	0.18391828092059845	2.2084327680187133E-5	0.0
62-63	0.21357753299508977	2.2084327680187133E-5	0.0
64-65	0.2500277158312386	2.2084327680187133E-5	0.0
66-67	0.28633435053746625	2.2084327680187133E-5	0.0
68-69	0.32420897250898717	2.2084327680187133E-5	0.0
70-71	0.36864263980152373	3.31264915202807E-5	0.0
72-73	0.42317988700774584	4.416865536037427E-5	0.0
74-75	0.4834921859023369	4.416865536037427E-5	0.0
76-77	0.546288971660949	4.416865536037427E-5	0.0
78-79	0.6186041026497218	4.416865536037427E-5	0.0
80-81	0.7030876981902776	4.416865536037427E-5	0.0
82-83	0.7955216516957009	4.416865536037427E-5	0.0
84-85	0.8958617945106311	4.416865536037427E-5	0.0
86-87	1.0061178004539655	4.416865536037427E-5	0.0
88-89	1.1297789932991733	4.416865536037427E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCAAT	530	0.0	72.11941	1
CGGAAGA	21450	0.0	63.754066	4
TCCAATC	600	0.0	63.691414	2
TCGGAAG	21785	0.0	63.057304	3
AGAGCAC	21770	0.0	62.52352	8
AAGAGCA	21935	0.0	62.127888	7
GATCGGA	22825	0.0	60.51111	1
GAGCACA	22595	0.0	60.467487	9
GCATTCC	455	0.0	59.399403	3
GAAGAGC	23945	0.0	56.95038	6
CCAGACG	360	0.0	55.64866	6
GGAAGAG	24780	0.0	55.299454	5
ATCGGAA	26350	0.0	52.29847	2
TCAAAGG	795	0.0	52.15803	7
CTTGTTA	650	0.0	51.633713	1
CAGACGT	405	0.0	50.61695	7
GGCCCTC	385	0.0	48.41919	2
CAATCAA	845	0.0	45.766586	4
CCGGCAT	145	0.0	45.006397	1
GTCACCG	6945	0.0	43.911724	28-29
>>END_MODULE