FastQCFastQC Report
Thu 14 Aug 2014
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4528098
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT683381.5091987850086284No Hit
CACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAAC323550.7145384220924547No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC271920.6005170382796485TruSeq Adapter, Index 2 (100% over 50bp)
CTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAA239820.5296263464262478No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA226530.5002762749392792No Hit
GGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAACCATTGCG175520.3876241194426446No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA149770.33075697566616274No Hit
CTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCT146640.32384458110226416No Hit
CCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTAC140170.30955602109318303No Hit
CTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCTT133930.2957754006207463No Hit
CAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTC124640.27525906020585245No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC123440.27260894088423TruSeq Adapter, Index 3 (100% over 50bp)
AAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCA118490.26167719868253736No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG113800.2513196490005296No Hit
CTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACT109540.24191172540876985No Hit
CTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAAC98270.21702268811319897No Hit
ATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTTCAACG95530.21097158232882768No Hit
CTTCAACCGGGCCTACGCCCAAAGTCTTCAACGCTAACCATTGCGACCCT94470.20863064359472788No Hit
CTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCAAGATC91670.20244703184427543No Hit
GTGGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGC91200.20140906844330667No Hit
ACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAG75080.16580913222284502No Hit
GTCAGATAGGTGATTAACGTTCACATCGAGATGTAACTTTATTGCATACA66710.14732454995452837No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA63180.1395287822834223No Hit
GCTACTACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGC62580.13820372262261107No Hit
TACCACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAA61150.13504566376434432No Hit
ATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCC60730.13411812200177647No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG58100.12830994382188723No Hit
CTTCAACGCTAACCATTGCGACCCTCTTACTCGTTGCGGCCAGATTTCCC56450.12466602975465636No Hit
CAAGGATCTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATG54510.12038167018470006No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA53800.11881368291940678No Hit
CACCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTC53680.11854867098724453No Hit
TTAACGTTCACATCGAGATGTAACTTTATTGCATACAATATTATAATATT52090.11503726288609478No Hit
GGAACCCTTCTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTA51690.11415388977888728No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC50810.11221046894303083No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG49470.10925116903388575No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGGATCTCGTATGC48090.10620353181401992TruSeq Adapter, Index 2 (98% over 50bp)
TTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCA47690.10532015870681245No Hit
TCTCACTTGAATATTTGCTACTACCACCAAGATCTGCACCAATGGAAGCT47060.10392884606296064No Hit
CCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCAA46910.10359758114775784No Hit
CCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCAAG46670.10306755728343335No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG46540.10278046102359091No Hit
TTTATTTTAAAAAACGGCACTAGCCAATATCTTAGGATCGACTGACCCAC45480.10043952228949107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT5300.072.119411
CGGAAGA214500.063.7540664
TCCAATC6000.063.6914142
TCGGAAG217850.063.0573043
AGAGCAC217700.062.523528
AAGAGCA219350.062.1278887
GATCGGA228250.060.511111
GAGCACA225950.060.4674879
GCATTCC4550.059.3994033
GAAGAGC239450.056.950386
CCAGACG3600.055.648666
GGAAGAG247800.055.2994545
ATCGGAA263500.052.298472
TCAAAGG7950.052.158037
CTTGTTA6500.051.6337131
CAGACGT4050.050.616957
GGCCCTC3850.048.419192
CAATCAA8450.045.7665864
CCGGCAT1450.045.0063971
GTCACCG69450.043.91172428-29
Produced by FastQC (version 0.11.2)