FastQCFastQC Report
Thu 14 Aug 2014
brugia_8_TGACCA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebrugia_8_TGACCA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42584535
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA2272040.5335364117513554No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC1690940.3970784229533092No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA1579520.3709139949514536No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT1371570.32208171346710723No Hit
GGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATGG1346720.3162462617004037No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT1279750.3005198953094122No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG1247400.2929232407962186No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC1224660.2875832740688609No Hit
CTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACAAA1222040.2869680272427537No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1206640.2833516909366276No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1186600.27864575719800627No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA1176270.2762199939485074No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1142860.2683744227804765No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG1133300.2661294763462839No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG1123010.2637131061780996No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1108250.2602470591730073No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA1100570.25844358756060154No Hit
CCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTAC1099050.2580866504706462No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC1055520.24786462973001822No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1039350.24406747660858574No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC985580.23144082705141666No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA974140.22875440579543724No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC940340.22081725208458894No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT916180.21514383097056244No Hit
CGGAACGCTAGTTGGTATCGTTTACGGTCAGAACTAGGGCGGTATCTGAT909700.21362215179759506No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA895360.21025473214630616No Hit
CGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACA880620.20679338168187114No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC871780.2047175107113416No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC823440.1933659719426313No Hit
GGACAAGCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGAT816170.1916587794137003No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT808480.18985295953096587No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT807920.1897214563925613No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG805100.18905924415988104No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT804260.18886198945227417No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC775760.18216941901561212No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC774310.1818289198179574No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC755600.17743530603304697No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT753480.17693747272337246No Hit
CTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAACAGAG750110.1761461056226163No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG749060.1758995372381077No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT746910.17539465911744723No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT746730.17535239025153146No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT725830.17044450526464597No Hit
GTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACCC724950.17023785747572448No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT722720.16971419319243478No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT715530.168025786826133No Hit
CTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCG714660.16782148730754015No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA709500.16660977981795502No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT704170.16535815173278282No Hit
CTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATG702300.16491902518132462No Hit
CTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTA698540.16403607553775096No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC693040.16274452685699162No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG685570.16099036892148758No Hit
ATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTG681880.1601238571702145No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA664930.15614353896314706No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG655280.1538774580959966No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT637260.14964587496376325No Hit
CCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGG636730.1495214166363446No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA636000.14934999290235293No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA632120.14843886401483544No Hit
CAGCAGTCTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGC626770.14718253938900588No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA622830.14625732087951648No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT613610.1440922156364981No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT611200.14352628248729263No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA608780.14295800106775852No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG603320.1416758454683138No Hit
ATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCA594470.13959762622745558No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG593240.13930878897703122No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG593210.13930174416604527No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT593070.13926886838144412No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC588750.13825441559946586No Hit
GTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATG582440.1367726570220856No Hit
CTGTGATGCCCTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAA574410.13488699594817696No Hit
CGATAACCGGCCTCAAAGAGAACGGTCTCGTTATTTTTCGTCACTACCTC573380.13464512410432566No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT569550.13374573656845143No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC562230.1320268026878772No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC554540.13022098280514277No Hit
GCAGTCTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGG551650.12954233268016194No Hit
GAAAATCCTCCGTGATCGGGATCGGGAATTGCAATTATTTCCCTTGAACG551580.12952589478786136No Hit
CGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACC541980.12727155527235418No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT533410.12525908760069823No Hit
CACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAA531850.1248927574294283No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC529320.12429864503627902No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC528170.12402859394848387No Hit
CGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTT521050.1223566254744827No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT517720.12157465145504114No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG516850.12137035193644828No Hit
CGGAGGTAGAGCGTATATGCTGGGACCCGAAAGATGGTGAACTATACCTG514210.12075040856968382No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA512750.12040756110170041No Hit
CGGGCAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGA509220.11957862167568578No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG504270.11841622786300239No Hit
ATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCTCGTTCAAGGG502790.11806868385436167No Hit
CTTTGTTCGATCCATGAAAATCCGAGGGAAGCTATTTGAATTTCGTGCCA494060.11601864385744731No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG489100.11485390177443525No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT483700.11358583579696244No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA479370.11256903474465554No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA477020.11201719121742201No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA476970.11200544986577875No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC473220.11112484849253373No Hit
CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC472850.11103796249037357No Hit
GCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCAG471700.11076791140257844No Hit
GAAAATCCGAGGGAAGCTATTTGAATTTCGTGCCAGATCGTACCCATATC470570.1105025568554406No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA469960.11035931236539273No Hit
GCGCTTTGTTCGATCCATGAAAATCCGAGGGAAGCTATTTGAATTTCGTG468940.1101197887918701No Hit
GCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTT460530.10814489344547264No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC451460.10601501225738405No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGC451220.10595865376949636No Hit
GCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTT449850.10563694073447087No Hit
GGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTC436230.102438596546845No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC435280.10221551086562293No Hit
GGGAATTGCAATTATTTCCCTTGAACGAGGAATTCCTAGTAAGTGTGAGT434050.10192667361519857No Hit
TTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAAGCT431240.10126680965284698No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG430460.101083644567212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT52350.078.33981
CCAGACG114650.067.364576
CAACCAG115350.065.3865053
CAGACGT120450.064.280167
TCCAATC68300.060.9581342
CCAATCA67650.060.691813
ATCAAAG69450.059.9439546
CAATCAA70500.059.251364
CTCCATT193900.058.1639671
CTCCCAC202200.057.5839921
CTTGTTA68750.057.235731
CCGGCAT15200.056.522371
CATTCCT207500.054.194074
CTCCGAT27200.053.923111
GTCAACT295450.053.274071
GCGTAAC56950.052.6755071
CAAAGGA79850.051.6662988
CCGGTAG11600.051.5582241
GATTGGG18300.051.09761
CGCGTAA10700.049.6843571
Produced by FastQC (version 0.11.2)