FastQCFastQC Report
Thu 14 Aug 2014
brugia_8_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebrugia_8_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42584535
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA2262900.5313900926709662No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC1649880.3874364249838586No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA1406440.33027013210312145No Hit
CTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACAAA1383600.3249066826724772No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1374280.3227180947261723TruSeq Adapter, Index 4 (100% over 50bp)
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT1337340.31404358413212685No Hit
GGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATGG1334120.31328744108630047No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG1310180.30766568191950433No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1243540.29201680844935846No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1235680.2901710679710369No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC1196620.2809987240673169No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG1140070.2677192553587822No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1126980.264645369498575No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1122720.2636450063385687No Hit
CCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTAC1090780.25614463090884987No Hit
CGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACA1089800.2559145004166419No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC1079350.2534605579231991No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT1033180.24261859381580658No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT1008760.23688411767323514No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC993130.23321377114954994No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA992910.23316210920231958No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC980180.23017276107394385No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG970600.22792311809909396No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA922170.21655044489742578No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC913510.214516842792812No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC911210.21397674061722172No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA908570.21335679725045723No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA866440.2034635343558407No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT835890.1962895685018047No Hit
CTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCG827160.19423952850489032No Hit
CGGAACGCTAGTTGGTATCGTTTACGGTCAGAACTAGGGCGGTATCTGAT821760.19297146252741754No Hit
GGACAAGCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGAT809300.19004551769791544No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC791670.18590551710849962No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT780080.18318387179759038No Hit
ATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTG777820.18265316270331472No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG770230.18087082552386682No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC770220.18086847725353816No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG768330.18042465416142267No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT764380.1794970873816046No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG763510.17929278786301178No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT760450.17857421714244384No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT747830.1756106999876833No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT743710.17464321261227816No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT742040.17425105146739303No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC738660.1734573360963082No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG736350.1729148856503893No Hit
GTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACCC731340.17173840221573394No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT718430.1687067852214425No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT709020.1664970628421797No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA691800.1624533413362386No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA686000.16109134454561969No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC680980.1599125128406357No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG675730.15867967091809268No Hit
CTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAACAGAG675660.15866323302579213No Hit
CTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATG671870.1577732385712325No Hit
CGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACC669570.15723313639564224No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT669140.15713216077151013No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT640010.15029164930414293No Hit
CAGCAGTCTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGC627190.14728116674280933No Hit
ATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCA623450.146402913639893No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA621040.14583698049068752No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG619460.1454659537787603No Hit
CTGTGATGCCCTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAA617680.14504796166026No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT613810.14413918104307114No Hit
GTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATG610270.14330789334672786No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT609820.14320222118193848No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT607760.14271847749423588No Hit
CGATAACCGGCCTCAAAGAGAACGGTCTCGTTATTTTTCGTCACTACCTC600650.14104885729056335No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT598410.14052284473694499No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA595240.13977844304276188No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA594260.13954831255055386No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA580560.13633118220029877No Hit
CGGGCAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGA578360.13581456272799503No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC577060.1355092875852701No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG565750.13285339384356315No Hit
GCAGTCTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGG563450.1323132916679729No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG561230.13179197565501183No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC560530.1316275967320061No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC559770.13144912818702845No Hit
CTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTA558590.13117203228824736No Hit
CCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGG557250.1308573640642078No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA549950.12914312672429087No Hit
GCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCAG548930.1289036031507682No Hit
CACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAA548860.12888716525846766No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG548350.12876740347170634No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC547040.12845978005865274No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT546570.12834941135320604No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA538520.12645905373864008No Hit
CTTTGTTCGATCCATGAAAATCCGAGGGAAGCTATTTGAATTTCGTGCCA533970.12539059073910283No Hit
CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC529860.12442545163402631No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC524950.1232724509026575No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA524270.12311276852030907No Hit
GCGCTTTGTTCGATCCATGAAAATCCGAGGGAAGCTATTTGAATTTCGTG519200.12192219546368183No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT517760.12158404453635574No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC510930.1199801759018855No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG510760.11994025530629841No Hit
GCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTT507370.11914419166488492No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGC504610.1184960690541766No Hit
TTTGAATTTCGTGCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGT503850.11831760050919893No Hit
ATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCTCGTTCAAGGG496070.11649064619350664No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG493400.1158636580157562No Hit
GCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTT493280.11583547877181234No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC492340.11561474136091894No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT485320.11396625559020428No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA484800.11384414553311431No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC480870.11292127529395354No Hit
CGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTT480330.11279446869620627No Hit
GTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTTCCATCTCAGCGGT479560.11261365188089995No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTTACATTCAGAGCAC479370.11256903474465554No Hit
GAAAATCCTCCGTGATCGGGATCGGGAATTGCAATTATTTCCCTTGAACG472060.11085244913440993No Hit
TTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAAGCT471030.11061057729055866No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT468110.10992488235459187No Hit
CGGAGGTAGAGCGTATATGCTGGGACCCGAAAGATGGTGAACTATACCTG463330.10880240913749557No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT462530.1086145475112033No Hit
CGGTAACGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTT461410.10835154123439412No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC436320.10245973097980288No Hit
GACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCTCGTT435650.1023023968677831No Hit
CGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGC435450.10225543146121005No Hit
CGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAGAA434440.10201825615801605No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC433970.10190788745256935No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA429290.10080889693875957No Hit
CTCGTTATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCG425860.10000344021603147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT50000.079.440311
CCGGCAT11750.071.9758451
CCAGACG114950.068.454976
ATCAAAG58750.067.6564256
CAACCAG112350.067.480183
CCGGTAG8150.066.458711
CGCGTAT3500.065.1595151
CAGACGT120850.065.152247
CGCGTAA10150.064.12971
CCAATCA61700.063.362323
TCCAATC64350.063.041382
CAATCAA62450.062.737064
CTTGTTA68250.058.963791
CTCCCAC196150.057.939871
CAAAGGA67950.057.657528
CTCCATT177600.056.6881981
ACATAAC58200.055.8932086
GCGTAAC60950.055.8141061
TCAAAGG74650.054.645557
CTCCGAT21850.053.926831
Produced by FastQC (version 0.11.2)