FastQCFastQC Report
Thu 14 Aug 2014
brugia_8_TGACCA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebrugia_8_TGACCA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43216767
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA2312980.535204310863883No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC1716230.39712133024666096No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA1614620.3736096223949376No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT1393600.3224674349194145No Hit
GGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATGG1368260.3166039699360204No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT1300830.301001229453374No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG1268410.2934995114280529No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC1256010.2906302546879548No Hit
CTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACAAA1250970.2894640406581085No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1237670.2863865314126806No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1217000.2816036655402751No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA1199570.27757050868705657No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1175820.27207495646307833No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG1154430.26712548858640905No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG1149470.2659777858903698No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1128020.2610144345133453No Hit
CCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTAC1122130.25965153756179865No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA1113900.2577471840963948No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC1065710.2465964193943522No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1051280.24325743755889928No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC1007610.233152563216957No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA1000380.2314796014241417No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC962040.22260804469709639No Hit
CGGAACGCTAGTTGGTATCGTTTACGGTCAGAACTAGGGCGGTATCTGAT935990.21658029162616443No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT933940.2161059386973579No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA913940.21147810524558674No Hit
CGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACA896600.2074657736429011No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC885860.20498062707930004No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC835490.19332542853101436No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT833440.19285107560220782No Hit
GGACAAGCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGAT831190.19233044433888358No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG820410.1898360421083789No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT818950.18949821026639962No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT813740.18829265965221323No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC785300.18171188048379464No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC774810.17928458183834065No Hit
CTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAACAGAG773170.17890509949529543No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT768560.17783838388466217No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT765430.17711412794946No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG762300.1763898720142578No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC761060.17610294634024798No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT755290.174767816389412No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT735460.1701793195219809No Hit
GTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACCC732820.16956844550634712No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT732300.16944812183660107No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT728770.16863130923236344No Hit
CTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCG728380.1685410664800539No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA720330.16667836351571602No Hit
CTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATG714980.16544041806736723No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT713030.16498920430581954No Hit
CTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTA711330.16459583846241901No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC704320.1629737828375732No Hit
ATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTG698350.16159237455221953No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG693160.1603914517714849No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG675010.1561916929140026No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA670280.1550972103026587No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT648740.15011303367510115No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA648680.15009915017474584No Hit
CCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGG647970.14993486208720797No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA645540.14937258032281775No Hit
CAGCAGTCTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGC637270.14745897119051038No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT627220.1451334848809954No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA624080.1444069150290673No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT622070.1439418177671643No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG617680.14292600832450053No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA613600.14198193030033923No Hit
ATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCA611810.1415677392064057No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG606060.14023723708902147No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT602900.13950603940364165No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC602750.13947133065275338No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG591570.1368843717532133No Hit
GTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATG591140.13678487333400022No Hit
CGATAACCGGCCTCAAAGAGAACGGTCTCGTTATTTTTCGTCACTACCTC588170.1360976400664122No Hit
CTGTGATGCCCTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAA587270.1358893875610825No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT584050.13514430637534733No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC566330.13104404593707808No Hit
GCAGTCTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGG562030.13004906174494726No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC561330.12988708757413528No Hit
GAAAATCCTCCGTGATCGGGATCGGGAATTGCAATTATTTCCCTTGAACG561190.12985469273997288No Hit
CGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACC552180.12776985376994998No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT551940.12771431976852873No Hit
CACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAA545980.1263352253999009No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC541290.1252499984554606No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC539840.12491448053020718No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG531850.12306566106622459No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT529070.12242239221642842No Hit
CGGAGGTAGAGCGTATATGCTGGGACCCGAAAGATGGTGAACTATACCTG526800.12189713311965239No Hit
CGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTT525400.12157318477802839No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA519480.12020334607630413No Hit
CGGGCAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGA518930.12007608065638042No Hit
ATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCTCGTTCAAGGG513030.11871086978810794No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG507190.11735954242019075No Hit
CTTTGTTCGATCCATGAAAATCCGAGGGAAGCTATTTGAATTTCGTGCCA503450.11649413756470955No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG497450.1151057875291782No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT492350.11392568999897656No Hit
CTCCACTTCAGTCTTCAAGGATCTCACTTGAATATTTGCTACTACCACCA491650.11376371582816455No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA487590.11282426563745503No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA486020.11246098071149098No Hit
GCGCTTTGTTCGATCCATGAAAATCCGAGGGAAGCTATTTGAATTTCGTG485720.11239156320971443No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC483020.11176680569372531No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA480410.11116287342826917No Hit
GCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCAG480340.11114667601118797No Hit
CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC480010.11107031675923376No Hit
GAAAATCCGAGGGAAGCTATTTGAATTTCGTGCCAGATCGTACCCATATC479420.1109337956724065No Hit
GCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTT472490.1093302513813678No Hit
GCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTT462460.10700939290530455No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC461880.1068751857352032No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGC456280.10557939236870728No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC446380.10328861481008055No Hit
GGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTC445540.10309424580510615No Hit
TTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAAGCT442540.10240007078734048No Hit
GGGAATTGCAATTATTTCCCTTGAACGAGGAATTCCTAGTAAGTGTGAGT440520.10193265960871159No Hit
GTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTTCCATCTCAGCGGT437560.10124774025784945No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG432220.10001210872622657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT53000.077.778781
CCAGACG117850.067.066126
CAACCAG117400.065.836653
CAGACGT124800.063.4102827
TCCAATC67400.063.257562
CCAATCA66350.062.4675833
ATCAAAG68850.061.5695656
CAATCAA68650.060.8542444
CCGGCAT15150.060.187441
CTCCATT195700.059.285691
CTCCCAC202450.058.083141
CGCGTAA10100.055.955511
CTTGTTA66400.055.6453361
CATTCCT208500.055.4195824
CCGGTAG11250.054.0349431
CGCGTAT3900.053.5803221
CTCCGAT27500.053.3633461
GCGTAAC61450.052.553821
CAAAGGA80250.052.2358178
GTCAACT307650.051.4203381
Produced by FastQC (version 0.11.2)