FastQCFastQC Report
Thu 14 Aug 2014
brugia_8_TGACCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebrugia_8_TGACCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43216767
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA2282280.5281005865154142No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC1673580.38725247541075897No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA1421480.3289186347511835No Hit
CTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACAAA1410930.3264774526053742No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT1355370.3136213312763539No Hit
GGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATGG1352730.31301045726072013No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG1329070.3075357302872749No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1300240.3008647083665467TruSeq Adapter, Index 4 (100% over 50bp)
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1259160.29135913845660877No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1245740.2882538622104703No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC1200040.27767926277317323No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG1152160.266600229489633No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1138530.263446360992251No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1136970.26308538998301284No Hit
CGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACA1110190.2568887209910913No Hit
CCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTAC1106180.2559608403840111No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC1085650.25121036934576807No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT1031420.23866199894129053No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT1030650.23848382735339735No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA1004650.23246764386609486No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC991010.22931146145198691No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC981750.22716877456381684No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG971100.2247044532507487No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC927320.21457412582482163No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA926910.21447925523906033No Hit
AAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGCA925150.21407200589530448No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC921010.21311404437078782No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA869240.2011348974808782No Hit
CTTTTCTGACACCTCTTGCTAGAAACCTTCTAAAAAATTCAAAAGGATCG841050.19461196623060675No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT840910.19457957139644436No Hit
CGGAACGCTAGTTGGTATCGTTTACGGTCAGAACTAGGGCGGTATCTGAT832000.1925178715936803No Hit
GGACAAGCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGAT818650.18942879276462304No Hit
ATTTTTCGTCACTACCTCCTCATTCTGAGAGTGGGTAATTTGCGCGCCTG793010.18349591027945242No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC791130.18306089393498592No Hit
GGCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCT784250.18146891922757666No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG780700.18064747878988727No Hit
CTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGT777150.17982603835219788No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG775860.17952754309455865No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC769470.17804895030671777No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG767140.1775098077095864No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT763550.1766791116049935No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT754550.17459658655169646No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT751880.17397877078588503No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT751450.17387927236667194No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC748720.17324757310050518No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG743160.1719610354009128No Hit
GTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACCC739990.17122752379880707No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT725340.1678376357953847No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT714230.1652668743129258No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA699840.16193714814437649No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA695110.16084266553303259No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC693240.16040996310529199No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG690830.15985230917435356No Hit
CTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAACAGAG683080.15805902371179223No Hit
CTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATG680930.15756153161572684No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT679920.15732782602641238No Hit
CGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACC677360.15673546334458568No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG638130.14765796802893655No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT636940.14738261193855617No Hit
CAGCAGTCTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGC629210.1455939543094466No Hit
CTGTGATGCCCTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAA628740.14548520022333No Hit
ATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTATCA628550.14544123580553817No Hit
ATCAAACCGTACTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGA622040.14393487601698665No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT620680.1436201833422662No Hit
CACGCTTTCGCGGTCTGTATTCGTACTGGAAATCAAGATCAAGCGAGCTT618370.14308566857858662No Hit
GTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATG615940.14252338681419646No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGT615570.14243777189533868No Hit
CGATAACCGGCCTCAAAGAGAACGGTCTCGTTATTTTTCGTCACTACCTC612650.14176210821138008No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA602630.13944356365204275No Hit
GGAAGTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAA600610.13897615247341386No Hit
CAACGGTTGAGTATAAGTCTCCCGCTCAAGCGCCATCCATTTTCAGGGCT600040.13884425922003837No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA587330.1359032710614378No Hit
CGGGCAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGA585560.13549370780095604No Hit
GAAAAAAGTTACGGTAGGTTATTCCTTGGAGTCGGGTTGTTTGAGATTGC578110.1337698398401713No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG575930.13326540599392825No Hit
GCAGTCTCCGCTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGG571850.13232132796976692No Hit
CCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGG570950.1321130754644372No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC567770.13137724994560562No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG567600.13133791336126555No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC563070.13028970908443938No Hit
CTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTTA561630.12995650507591186No Hit
GCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCAG561380.12989865715776472No Hit
CACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAA556330.1287301292111925No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG554970.12841543653647206No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA554810.1283784138688579No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT554170.12823032319840122No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC553450.12806372119413745No Hit
CTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCA545990.1263375393166268No Hit
CTTTGTTCGATCCATGAAAATCCGAGGGAAGCTATTTGAATTTCGTGCCA540830.12514355828606985No Hit
CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC537790.12444012760140064No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC533270.12339423724130034No Hit
GCGCTTTGTTCGATCCATGAAAATCCGAGGGAAGCTATTTGAATTTCGTG530700.12279956064274776No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA528250.1222326510449058No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT522800.12097156642929814No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG519550.12021954349338533No Hit
TTTGAATTTCGTGCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGT514470.11904407379663545No Hit
GCTGATCGCATGGTCTTGTACCGGCGACGTATCTATCAAGTGTCTGCCTT513360.11878722904006214No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAAACACAGC506310.11715591774831283No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC505850.11704947757892209No Hit
CTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTACTTGTTTGCTATC502360.11624192064158803No Hit
ATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCTCGTTCAAGGG499630.11561022137542126No Hit
GCGGTGTTTAGCCGCATGAAGTTGAGCAATAACAGGTCTGTGATGCCCTT497250.1150595091946605No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG494960.11452962226443268No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC491720.11377991324524575No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA491370.11369892615983976No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT488050.11293070580684575No Hit
GTTGAATACCGATAAGTTTTGTTAACTCTATCCGTTTCCATCTCAGCGGT487740.1128589743883433No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTTACATTCAGAGCAC483330.11183853711222776No Hit
CGGCGACGTATCTATCAAGTGTCTGCCTTATCAACTTTCGATGGTAGTTT482020.11153541402113676No Hit
CTCGGATTTTCATGGATCGAACAAAGCGCACGAGACACCACAGGAACCGT474160.1097166754745907No Hit
CGGTAACGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTT472130.10924695037923592No Hit
TTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAAGCT471310.1090572092077133No Hit
GAAAATCCTCCGTGATCGGGATCGGGAATTGCAATTATTTCCCTTGAACG465650.10774753234086204No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT463630.10728012116223316No Hit
CGGAGGTAGAGCGTATATGCTGGGACCCGAAAGATGGTGAACTATACCTG463360.10721764541063426No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC442790.10245791870548762No Hit
CGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAGACATTCTTGGC442350.10235610636954864No Hit
CGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAGAA441930.10225892186706145No Hit
GACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCTCGTT441850.10224041053325436No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC435720.1008219795802865No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA434810.10061141315823093No Hit
CCCACATTCAACTGCTGTTCACGTGGAACCCTTCTCCACTTCAGTCTTCA433110.10021804731483039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT50750.078.446371
CCGGCAT13300.069.297091
ATCAAAG60500.066.878936
CCAGACG113300.066.8133856
CCAATCA60900.065.903933
CAACCAG113600.064.640263
CAGACGT117550.064.397767
CAATCAA63250.063.6770744
TCCAATC64900.062.793472
CGCGTAT3750.059.5431061
CGCCCTT19050.059.3535731
CTTGTTA69150.058.5345461
CTCCGAT26250.058.095251
CTCCCAC200500.057.9571461
CAAAGGA69500.057.8766868
CTCCATT174950.057.785991
CCGGTAG6450.057.451291
CGCGTAA9800.057.2032661
CTCGACT4000.057.0093571
GCGTAAC59650.056.0695531
Produced by FastQC (version 0.11.2)