FastQCFastQC Report
Thu 14 Aug 2014
brugia_4_TTAGGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebrugia_4_TTAGGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39291846
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC7234161.841135181075483TruSeq Adapter, Index 3 (100% over 50bp)
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA2475030.6299093201169526No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC1336690.3401952659592527No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG1226960.31226835206470066No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT1155530.2940890076785906No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG1133750.28854587285107447No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1115000.28377389038936984No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1107990.28198980521302053No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC976510.2485273916628911No Hit
CTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAACAGAG969330.2467000405122223No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA932070.23721715696432283No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA870060.22143525656697322No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC862520.21951628335303972No Hit
CTCCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAG838210.21332924902535758No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC819560.20858271713678203No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT817850.20814751233627454No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT793500.20195029777934076No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA749110.1906527883673371No Hit
GTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACCC744370.18944643120101815No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA738920.18805937496548267No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT733470.18667231872994716No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC728280.18535143398454731No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC717110.1825086049660278No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT711130.18098666069290814No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA692590.1762681244347746No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC687950.17508721784158474No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT672100.17105330200062374No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG667110.16978331840148206No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA663860.1689561747747866No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC655930.16693794432564965No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC654200.16649764941051637No Hit
GTTAATATTCCCGTACCTGGACACGGAGATAAATGCTCATCGTGAGAATT653350.1662813195389191No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC645600.1643089001214145No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG643780.16384569969046503No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT636140.16190127590340248No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG606240.1542915545378041No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA594880.1514003694303393No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC582980.1483717512279774No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTTACATTCAGAGCAC582130.14815542135638016No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA574360.14617791182424975No Hit
CTCCATTCCTCTATTTTATTTTAAAAAACGGCACTAGCCAATATCTTAGG571310.14540166934381243No Hit
GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC557330.14184367922036545No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT548440.13958112326918923No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT545890.1389321336543974No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA539400.13728039145831936No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA534250.13596968694217115No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG520080.1323633407297789No Hit
GGGGAATCCGACTGTCTAATTAAAACAGAGGAGACAGATGGCCCTTGCGG516280.13139621895087342No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT508410.12939325884561392No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT503390.12811564007453352No Hit
GGAAAACTGGCAATATTAGCCTAGGAGCCTAGTTCTCATCCAATGGATGG502860.12798075203694934No Hit
GTTTACCCGCGCTTGGTTGAATTTCTTCACTTTTACATTCAGAGCACTGG494920.12595997653049948No Hit
GCCGCAACGAGTAAGAGGGTCGCAATGGTTAGCGTTGAAGACTTTGGGCG486760.12388320976316562No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG481240.12247833812643978No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT479360.12199986735161285No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC468980.11935809786081317No Hit
GTTGGATCTGCGTCTCAGGACCTGGTCCATCCATTGGATGAGAACTAGGC468960.11935300774618735No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT465750.1185360443487435No Hit
CTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACAAA464640.11825354298701059No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG464430.1182000967834395No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG463980.11808556920435859No Hit
CGTTCATGTTGCATGCAATAGGGCGGAGGTAGAGCGTATATGCTGGGACC461650.11749257085045076No Hit
TTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTCAAGCT460190.117120992482766No Hit
CCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTTACATTCAGAGC458310.1166425217079391No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA456640.11621749713668326No Hit
TTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCCACTTAT453870.11551251626100743No Hit
GTTCGATTAGTCTTTCGCCCCTATACCAAAGTCAGACGATCGATTTGCAC452650.11520201926883253No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC451800.11498568939723525No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG446790.11371061568346776No Hit
GCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTCTTCATTAATCA444320.11308198652717921No Hit
CGATAACCGGCCTCAAAGAGAACGGTCTCGTTATTTTTCGTCACTACCTC429650.10934838744914148No Hit
GCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGT429300.10925931044318968No Hit
CTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAGAAT428250.1089920794253342No Hit
GTTGGAAACCGCTAAGGAGTGTGTAACAACTCACCTGCCAAATCAACTAG427750.10886482655968874No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG426200.10847034267618783No Hit
GTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTTACAT425400.1082667380911551No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC423540.10779335743095399No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC422540.10753885169966308No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC422110.10742941423520799No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA420780.10709092161259107No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT414590.10551553113590031No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC414460.1054824453908325No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC412930.10509305162195738No Hit
CCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGG410170.10439061580359446No Hit
GTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCTCGTGCGCTTTGTTCGA409410.10419719144781336No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC405020.10307991128744626No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG404250.10288394187435224No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA403690.10274141866482933No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG401510.10218659617061514No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA397850.10125510519409041No Hit
CTAGAGTCTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAAC397420.1011456677296353No Hit
CTCACGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTGCCC397130.10107186106756093No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAGTCGAACCCTT393940.10025998778474292No Hit
GTTAAATCACTCGGTGATTTAACGACTCGCACCGAAGACAGTGTCAGGCG393170.10006401837164892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAATC108600.080.836452
GTCCAAT111650.078.6466141
CCAATCA131100.067.506443
ATCAAAG133050.067.102256
CAATCAA142200.062.6865354
CTTCCGA42450.062.2348861
GGCGGTT21650.061.891071
CTTGTTA133100.061.3312641
CAAAGGA148250.059.7097638
CCAGACG56500.053.5408336
CCGGCAT12400.053.2635271
TCAAAGG179750.052.627727
CAGACGT59450.050.804177
TGGCTCA44900.050.678563
GTTACGA161100.050.5566374
TTACGAC164450.049.5267525
GCGAGCG23050.048.649441
GCGTAAC71500.046.319471
CGCGGAT13700.044.7410241
CTGCTTG841800.044.46970456-57
Produced by FastQC (version 0.11.2)