##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	brugia_2_CGATGT_L002_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48539493
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89211996919704	33.0	33.0	33.0	22.0	33.0
2	30.5653296172665	33.0	33.0	33.0	22.0	33.0
3	30.372081368876266	33.0	33.0	33.0	22.0	33.0
4	33.89424149939102	37.0	37.0	37.0	27.0	37.0
5	33.937358369194335	37.0	37.0	37.0	27.0	37.0
6	34.33889808037344	37.0	37.0	37.0	27.0	37.0
7	33.864474769029826	37.0	37.0	37.0	27.0	37.0
8	34.22258120825448	37.0	37.0	37.0	27.0	37.0
9	33.78162404786552	37.0	37.0	37.0	27.0	37.0
10-11	33.880465387226025	37.0	37.0	37.0	27.0	37.0
12-13	33.785426982107126	37.0	37.0	37.0	22.0	37.0
14-15	35.47968944587039	40.0	37.0	40.0	22.0	40.0
16-17	35.439437233100065	40.0	37.0	40.0	22.0	40.0
18-19	35.45782790726718	40.0	37.0	40.0	22.0	40.0
20-21	35.38001048960277	40.0	37.0	40.0	22.0	40.0
22-23	35.88071267040222	40.0	37.0	40.0	24.5	40.0
24-25	35.42552728146542	40.0	37.0	40.0	22.0	40.0
26-27	35.41925622296879	40.0	37.0	40.0	22.0	40.0
28-29	35.088738607137905	40.0	37.0	40.0	18.5	40.0
30-31	35.090197511951764	40.0	37.0	40.0	22.0	40.0
32-33	34.879366539737035	40.0	37.0	40.0	15.0	40.0
34-35	34.77313309597197	40.0	37.0	40.0	15.0	40.0
36-37	34.76719441630756	40.0	37.0	40.0	15.0	40.0
38-39	34.78467795285789	40.0	37.0	40.0	15.0	40.0
40-41	34.76330708687048	38.5	37.0	40.0	18.5	40.0
42-43	34.57221404228511	37.0	35.0	40.0	15.0	40.0
44-45	34.610118496705354	37.0	33.0	40.0	22.0	40.0
46-47	34.09273999833496	37.0	33.0	40.0	15.0	40.0
48-49	33.90047526866422	37.0	33.0	40.0	15.0	40.0
50-51	33.6424151463634	37.0	33.0	40.0	15.0	40.0
52-53	33.514589738298255	37.0	33.0	40.0	15.0	40.0
54-55	33.2989581081945	37.0	33.0	40.0	15.0	40.0
56-57	33.29413760048956	37.0	33.0	40.0	15.0	40.0
58-59	32.87686700806702	37.0	33.0	37.0	15.0	40.0
60-61	33.38921842467535	37.0	33.0	37.0	22.0	40.0
62-63	33.392671169845144	37.0	33.0	37.0	22.0	40.0
64-65	33.194193385991895	37.0	33.0	37.0	22.0	40.0
66-67	33.13218220058458	37.0	33.0	37.0	22.0	38.5
68-69	32.747523465067914	37.0	33.0	37.0	22.0	37.0
70-71	32.284250228983645	37.0	33.0	37.0	15.0	37.0
72-73	32.1100329890137	37.0	33.0	37.0	15.0	37.0
74-75	31.99918390165303	37.0	33.0	37.0	15.0	37.0
76-77	31.897727434029854	37.0	33.0	37.0	15.0	37.0
78-79	31.828992023052237	37.0	33.0	37.0	15.0	37.0
80-81	31.758731822765434	37.0	33.0	37.0	15.0	37.0
82-83	31.668622043497653	37.0	33.0	37.0	15.0	37.0
84-85	31.604160554375795	37.0	33.0	37.0	15.0	37.0
86-87	31.52957399039994	37.0	33.0	37.0	15.0	37.0
88-89	31.425803283524203	37.0	33.0	37.0	15.0	37.0
90-91	31.370668869573898	37.0	33.0	37.0	15.0	37.0
92-93	31.2832607254468	37.0	33.0	37.0	15.0	37.0
94-95	31.245827093826463	33.0	33.0	37.0	15.0	37.0
96-97	31.130796164269782	33.0	33.0	37.0	15.0	37.0
98-99	31.060103790124053	33.0	33.0	37.0	15.0	37.0
100-101	30.49135717177763	33.0	30.0	37.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.06779242533292162
2201	2	0.14460646479245298
2201	3	0.2191738402046397
2201	4	0.22918676382630565
2201	5	0.3018631983780793
2201	6	0.2168747781320235
2201	7	0.19084534016099752
2201	8	0.16348859560996232
2201	9	0.21688273761026977
2201	10-11	0.2812170240331753
2201	12-13	0.22100886267973152
2201	14-15	0.4413286325347414
2201	16-17	0.522631298773895
2201	18-19	0.48060255871281043
2201	20-21	0.4815379069790282
2201	22-23	0.5102278118182895
2201	24-25	0.5668032652954764
2201	26-27	0.5274950417417585
2201	28-29	0.6577720569175085
2201	30-31	0.5608717437285549
2201	32-33	0.6058764273036275
2201	34-35	0.6205843906263979
2201	36-37	0.6024062717087375
2201	38-39	0.5583816745715069
2201	40-41	0.5351348284733319
2201	42-43	0.552586270331588
2201	44-45	0.522072931147811
2201	46-47	0.5578328478723122
2201	48-49	0.5467746328039169
2201	50-51	0.6375793767905975
2201	52-53	0.5555495575326006
2201	54-55	0.5662870014844188
2201	56-57	0.5560778916916433
2201	58-59	0.585415930582819
2201	60-61	0.5563477941387234
2201	62-63	0.6402288019374893
2201	64-65	0.6204618234904729
2201	66-67	0.5993423929269213
2201	68-69	0.6238516601968627
2201	70-71	0.5771984626908093
2201	72-73	0.5529592456778047
2201	74-75	0.5493163641124212
2201	76-77	0.5529348710970794
2201	78-79	0.5786271122307234
2201	80-81	0.581620075436831
2201	82-83	0.5499875670864327
2201	84-85	0.5300354044012607
2201	86-87	0.510100886599389
2201	88-89	0.5186875835047786
2201	90-91	0.5111406753204442
2201	92-93	0.552870399190855
2201	94-95	0.5906527417990795
2201	96-97	0.5798078143336944
2201	98-99	0.5665911537715793
2201	100-101	0.6746627500960898
1101	1	0.1855473814963169
1101	2	0.23697568439083838
1101	3	0.26072445882073225
1101	4	0.3559719631456275
1101	5	0.251298766618099
1101	6	0.31188070779613497
1101	7	0.37048768056205716
1101	8	0.28804234580741195
1101	9	0.3528458165736126
1101	10-11	0.3376200283646682
1101	12-13	0.3604790037103456
1101	14-15	0.5655601367938559
1101	16-17	0.33197311977804134
1101	18-19	0.4821784982274764
1101	20-21	0.5005574033272637
1101	22-23	0.5377850368177448
1101	24-25	0.49915957044960635
1101	26-27	0.511079146256229
1101	28-29	0.4771724240434523
1101	30-31	0.43077951785818414
1101	32-33	0.44356516421843395
1101	34-35	0.5203448711630401
1101	36-37	0.5511177477276519
1101	38-39	0.4850783408523611
1101	40-41	0.4391190178507287
1101	42-43	0.4463255137362765
1101	44-45	0.5398741339652418
1101	46-47	0.5382169958246266
1101	48-49	0.5711081813688139
1101	50-51	0.5784967218723409
1101	52-53	0.5345175278503262
1101	54-55	0.5455926541484857
1101	56-57	0.5497452858446437
1101	58-59	0.5955151545085187
1101	60-61	0.5902395182424627
1101	62-63	0.6418671170928363
1101	64-65	0.5976710848912106
1101	66-67	0.5949572483268213
1101	68-69	0.6445177812556366
1101	70-71	0.5759454191100701
1101	72-73	0.5494009744657262
1101	74-75	0.5815027830617936
1101	76-77	0.5324456521262917
1101	78-79	0.5548820391111668
1101	80-81	0.503397303371024
1101	82-83	0.5011849229557654
1101	84-85	0.35349949958860805
1101	86-87	0.47653731667971044
1101	88-89	0.5799370976584974
1101	90-91	0.5543867063757659
1101	92-93	0.6108490324609548
1101	94-95	0.5552731588671982
1101	96-97	0.6201851131061318
1101	98-99	0.6292546708094093
1101	100-101	0.5813328970880391
2202	1	0.17962676999194116
2202	2	0.2613614611682813
2202	3	0.2694679546058545
2202	4	0.3088540174527239
2202	5	0.33539435747825763
2202	6	0.2930243076834671
2202	7	0.3289905886690079
2202	8	0.3530300969902598
2202	9	0.36149780492363703
2202	10-11	0.3642940796089391
2202	12-13	0.39173513149487604
2202	14-15	0.5190936220996392
2202	16-17	0.5850143526032809
2202	18-19	0.560889331281075
2202	20-21	0.5464489566002442
2202	22-23	0.5424632339720645
2202	24-25	0.48969946627489946
2202	26-27	0.4891820290971154
2202	28-29	0.6029706148006753
2202	30-31	0.5258328008337045
2202	32-33	0.5715300956992166
2202	34-35	0.5654089739160355
2202	36-37	0.5955222042178363
2202	38-39	0.5588240702152021
2202	40-41	0.5607440941183768
2202	42-43	0.5091468986496324
2202	44-45	0.4869874882092873
2202	46-47	0.49245577935695906
2202	48-49	0.5312852284318268
2202	50-51	0.5211242831093443
2202	52-53	0.5454258302450228
2202	54-55	0.565075558287738
2202	56-57	0.5804097709130573
2202	58-59	0.6145184226910274
2202	60-61	0.5562500897867508
2202	62-63	0.5565177242736112
2202	64-65	0.6355716506464191
2202	66-67	0.5752387220278976
2202	68-69	0.6162227016869863
2202	70-71	0.5614118456891006
2202	72-73	0.5101886619088987
2202	74-75	0.45673452240881574
2202	76-77	0.42868073802615214
2202	78-79	0.4949017259560726
2202	80-81	0.5485377742868209
2202	82-83	0.4117043826526796
2202	84-85	0.4243735067949217
2202	86-87	0.5120027006665069
2202	88-89	0.3624364125568249
2202	90-91	0.24854447296606352
2202	92-93	0.3660205946494486
2202	94-95	0.4191566188657667
2202	96-97	0.4156967591137608
2202	98-99	0.45182972011609834
2202	100-101	0.5154526769563788
1102	1	0.17804532046393362
1102	2	0.19031443417291172
1102	3	0.17359348788473028
1102	4	0.24853469232333936
1102	5	0.24096427219021876
1102	6	0.2332658553266711
1102	7	0.2403315191984703
1102	8	0.23990843951271756
1102	9	0.25701117487768954
1102	10-11	0.23797270593371422
1102	12-13	0.25375533920421134
1102	14-15	0.3518314832890397
1102	16-17	0.4063996904150571
1102	18-19	0.35076073356127324
1102	20-21	0.3755678728157932
1102	22-23	0.40971199159698557
1102	24-25	0.35709599051405405
1102	26-27	0.4555294408564379
1102	28-29	0.41917432998730675
1102	30-31	0.419725083204078
1102	32-33	0.3737774704539092
1102	34-35	0.36678222130596794
1102	36-37	0.41308418630592314
1102	38-39	0.37803283919421915
1102	40-41	0.3687283841608249
1102	42-43	0.4557034949768095
1102	44-45	0.4236590910192035
1102	46-47	0.4296262567414857
1102	48-49	0.42051104522312244
1102	50-51	0.42967376938898383
1102	52-53	0.3523086978947134
1102	54-55	0.40639711777345866
1102	56-57	0.4129666537802308
1102	58-59	0.4985022154005705
1102	60-61	0.42862839968311306
1102	62-63	0.4693363343929704
1102	64-65	0.4331169128193082
1102	66-67	0.42464789112795387
1102	68-69	0.4035660567249266
1102	70-71	0.42523116467646105
1102	72-73	0.39668790082743044
1102	74-75	0.4279315335261984
1102	76-77	0.42311299695720805
1102	78-79	0.3970567484819476
1102	80-81	0.3684703396888054
1102	82-83	0.2911372908686616
1102	84-85	0.3017113097643218
1102	86-87	0.376304273966344
1102	88-89	0.38471762581814417
1102	90-91	0.3726509845119743
1102	92-93	0.417259738978224
1102	94-95	0.35568888798183806
1102	96-97	0.4149553857286037
1102	98-99	0.4299236607090684
1102	100-101	0.37114388282518007
2203	1	0.16369061567692356
2203	2	0.0954657444619329
2203	3	0.15831732189386827
2203	4	0.18644682048211791
2203	5	0.24449766435927245
2203	6	0.22455359158769994
2203	7	0.19879842709307383
2203	8	0.20334096601670382
2203	9	0.2219123426729226
2203	10-11	0.15645151877399854
2203	12-13	0.2071790911503939
2203	14-15	0.28619574907078515
2203	16-17	0.3385425812585794
2203	18-19	0.32150158247497984
2203	20-21	0.2813296537664769
2203	22-23	0.24795625182437675
2203	24-25	0.2622139115217266
2203	26-27	0.2715605487346835
2203	28-29	0.27413311418803943
2203	30-31	0.23075740318317628
2203	32-33	0.2988864886954872
2203	34-35	0.2595626481922366
2203	36-37	0.2515476806864072
2203	38-39	0.22971501714072673
2203	40-41	0.2266660097606561
2203	42-43	0.19822396485126603
2203	44-45	0.23379681272642472
2203	46-47	0.2577540689657809
2203	48-49	0.18839162312666957
2203	50-51	0.24400390019201268
2203	52-53	0.2443356563397785
2203	54-55	0.2432719634728855
2203	56-57	0.24208863336161102
2203	58-59	0.2490521410174793
2203	60-61	0.2695890196735249
2203	62-63	0.2716602411457245
2203	64-65	0.2183520662569336
2203	66-67	0.19189244168231312
2203	68-69	0.14764991594195465
2203	70-71	0.1863512901805322
2203	72-73	0.1547355299357278
2203	74-75	0.18723850056033342
2203	76-77	0.1950590250559756
2203	78-79	0.2383735126175175
2203	80-81	0.10686301383376673
2203	82-83	0.14996646379106693
2203	84-85	0.08582808496100114
2203	86-87	0.20431779086662516
2203	88-89	0.17410219212802858
2203	90-91	0.20189859583658176
2203	92-93	0.2213492118079614
2203	94-95	0.15599133174431046
2203	96-97	0.02781976974896949
2203	98-99	0.1307648079288235
2203	100-101	0.10405672107354746
1103	1	0.1612833884962832
1103	2	0.15949378587030694
1103	3	0.17708269454098868
1103	4	0.25337014229944543
1103	5	0.26566351080074213
1103	6	0.2749695082914627
1103	7	0.24813547612802012
1103	8	0.23682827601243872
1103	9	0.24213408941881198
1103	10-11	0.25965843625262863
1103	12-13	0.2743750750025029
1103	14-15	0.3546414244186238
1103	16-17	0.3982400807637845
1103	18-19	0.3236970041061298
1103	20-21	0.32414591019344385
1103	22-23	0.35848095414394976
1103	24-25	0.33428046954600177
1103	26-27	0.4078533683078547
1103	28-29	0.3607096598771662
1103	30-31	0.3311154454530225
1103	32-33	0.33282729823493185
1103	34-35	0.39813112128404526
1103	36-37	0.3731123124861142
1103	38-39	0.34582092569252154
1103	40-41	0.36381692057915416
1103	42-43	0.36075119569269987
1103	44-45	0.3506916894854655
1103	46-47	0.31716071019050673
1103	48-49	0.3304229081769776
1103	50-51	0.4251823131367729
1103	52-53	0.40583950779912925
1103	54-55	0.3861092228222063
1103	56-57	0.4107705679366944
1103	58-59	0.38350868291734486
1103	60-61	0.40040248183047566
1103	62-63	0.40020725987613304
1103	64-65	0.4315086055098263
1103	66-67	0.3463401729200868
1103	68-69	0.34713114508325305
1103	70-71	0.2727413907494025
1103	72-73	0.3283939102172724
1103	74-75	0.2656695620559617
1103	76-77	0.2543635948886944
1103	78-79	0.22243483893965177
1103	80-81	0.23404724092325324
1103	82-83	0.25816094614171803
1103	84-85	0.13862949795253243
1103	86-87	0.24222935067176365
1103	88-89	0.3489884084399435
1103	90-91	0.3131958610419403
1103	92-93	0.3490514671873086
1103	94-95	0.35432265789361495
1103	96-97	0.2730972132945304
1103	98-99	0.25995232576811844
1103	100-101	0.27733842060720093
2204	1	0.1457986645465077
2204	2	0.1234541177212094
2204	3	0.07301957181879715
2204	4	0.14334596393206311
2204	5	0.18785452124205904
2204	6	0.1893881247126643
2204	7	0.13657880081433404
2204	8	0.12676830423859542
2204	9	0.1454953114391344
2204	10-11	0.17970278344424173
2204	12-13	0.13278770382810734
2204	14-15	0.2075112444707159
2204	16-17	0.1834757864863974
2204	18-19	0.14680878142149822
2204	20-21	0.12362521329750109
2204	22-23	0.03796910468827974
2204	24-25	-0.18540224467761846
2204	26-27	-0.12242104257512665
2204	28-29	-0.28771846270068124
2204	30-31	-0.11241234104783615
2204	32-33	-0.17661008802364364
2204	34-35	-0.25380795026487846
2204	36-37	-0.16008535788653688
2204	38-39	-0.08655588386233148
2204	40-41	0.029835546127358725
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1213	64-65	-2.0573550386639496
1213	66-67	-1.8028348222850283
1213	68-69	-1.5912956603747617
1213	70-71	-1.4794009517600557
1213	72-73	-1.5078646528746091
1213	74-75	-1.4922988034193203
1213	76-77	-1.3035443381922605
1213	78-79	-1.4056303531082612
1213	80-81	-1.373258783780436
1213	82-83	-1.3965771896748294
1213	84-85	-1.308255635677888
1213	86-87	-1.533895235884195
1213	88-89	-1.486478602026839
1213	90-91	-1.668256972673042
1213	92-93	-1.8397096895726044
1213	94-95	-1.7808540366549828
1213	96-97	-1.7315957001894162
1213	98-99	-1.8122657715346584
1213	100-101	-2.0657184619250444
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4065.0
3	18112.0
4	14218.0
5	3816.0
6	2884.0
7	10347.0
8	57016.0
9	248125.0
10	1039107.0
11	1556854.0
12	621489.0
13	244715.0
14	163160.0
15	140110.0
16	136178.0
17	141780.0
18	155091.0
19	174478.0
20	197269.0
21	223171.0
22	251408.0
23	282523.0
24	316344.0
25	359340.0
26	416140.0
27	488591.0
28	578733.0
29	695507.0
30	845018.0
31	1059519.0
32	1385026.0
33	1864865.0
34	2519027.0
35	3512565.0
36	6159996.0
37	1.5914486E7
38	6735766.0
39	2654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.794207056600186	11.783422318477541	11.967315010461013	36.45505561446126
2	21.30873376193595	23.388872263867682	37.93974996123844	17.362644012957926
3	22.944876589339135	24.862782141812275	26.08835584233856	26.10398542651002
4	24.11191226796089	34.818053429104566	19.073949752079383	21.99608455085516
5	26.80256637403185	32.444901766406076	20.6927191416419	20.059812717920174
6	21.96555962776715	33.84974254236027	25.595989806743418	18.58870802312916
7	18.766048053100857	16.21182023645831	41.74683021296085	23.275301497479987
8	18.020956409362675	23.39678752808318	28.05098022792348	30.531275834630662
9	26.604865185204606	23.080455892131425	27.267099886753144	23.04757903591082
10-11	25.512420997824393	33.92499461725975	19.624136091836185	20.938448293079674
12-13	26.034255826591874	22.351316963036776	27.89017575697297	23.724251453398384
14-15	24.8561531803822	25.27615535037863	27.138957666281648	22.728733802957517
16-17	27.067342301847557	25.273896265909247	26.130595841908182	21.528165590335018
18-19	26.468246370286273	25.01185500326011	25.658160412344603	22.861738214109018
20-21	25.77172845269532	26.337125887825163	25.65698022697343	22.234165432506085
22-23	28.40830797847767	25.353636536767205	24.252216570594687	21.985838914160443
24-25	24.901363978286188	28.74490195248497	24.51721422816776	21.836519841061087
26-27	23.884298448018633	29.587415075960717	25.29369846928593	21.234588006734718
28-29	24.759640765626674	28.06141662489992	24.398881837737974	22.780060771735428
30-31	27.175988075691105	25.716152576126223	24.836913469647936	22.270945878534736
32-33	23.940239484385284	27.34247191734488	25.739834605720702	22.977453992549137
34-35	23.26900981769684	29.40118502094397	25.693546384168346	21.636258777190847
36-37	22.061354853219925	28.275469481735836	26.950928366407656	22.71224729863658
38-39	21.954573036548688	25.26557974562207	28.742731403637343	24.037115814191896
40-41	26.769210271444084	26.16015942451297	25.669860623267116	21.40076968077583
42-43	27.016846553128225	25.42448047081488	26.03982936454149	21.518843611515404
44-45	27.678862325931075	26.301009277582306	25.761428463013576	20.258699933473046
46-47	26.971696357449275	24.66049247048336	25.507891462689635	22.859919709377728
48-49	23.490700816079613	25.169330531336286	25.01760360016033	26.32236505242377
50-51	25.665337116856662	25.258773627556973	25.580993477390145	23.494895778196216
52-53	23.65110102050855	27.059732324565534	27.511082423576656	21.778084231349265
54-55	22.8708841500077	26.940128541937963	25.966964622110574	24.222022685943763
56-57	21.471297794776923	25.58691969352534	30.640897791410755	22.300884720286984
58-59	22.515516961468947	28.89676842476164	27.499757694921417	21.087956918848004
60-61	22.25807656158641	31.723025757777517	25.187376179305538	20.83152150133053
62-63	21.111011101710364	32.12701233311851	25.323686532871825	21.4382900322993
64-65	21.285945122010958	32.22828226224521	24.887598162696882	21.598174453046955
66-67	22.88353060216486	31.759598350997887	23.976499644039468	21.38037140279779
68-69	21.573440131474662	30.9152214615461	25.993361674487108	21.517976732492127
70-71	22.08693102746258	29.33395580663014	25.93131477936277	22.647798386544512
72-73	22.948729974964717	29.30401657644346	25.094872374743627	22.652381073848197
74-75	23.251225494340385	29.041192908245648	26.247514925704905	21.460066671709054
76-77	23.67520579924457	27.678015050097194	25.94023930284259	22.706539847815645
78-79	23.878945499112202	27.880244609079195	25.059736007362172	23.18107388444643
80-81	24.98317966446045	27.28484441250189	25.54532890104808	22.18664702198958
82-83	24.120179655388522	27.458286129818273	25.757859639756276	22.663674575036925
84-85	23.792917806457137	28.370443278411393	24.782233065576065	23.0544058495554
86-87	22.829697592935474	29.097691852093057	25.666881977124884	22.405728577846588
88-89	23.258483495702308	27.94408350324976	26.91902849682901	21.878404504218913
90-91	24.38927401977917	26.98387114486654	26.55700992670652	22.069844908647767
92-93	23.347780952457168	26.7365749712963	27.6660019392375	22.249642137009033
94-95	23.679088532792505	27.188654371522702	25.55272659614231	23.579530499542482
96-97	22.732235662033652	29.44160679411333	25.330211493353872	22.495946050499143
98-99	24.026068643929623	28.684514713967534	25.417880053519216	21.871536588583627
100-101	23.55953406705764	27.406806829295547	25.806085193735743	23.227573909911065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3959.0
1	3898.5
2	5463.0
3	8602.5
4	10127.0
5	9015.5
6	6698.0
7	4794.0
8	3909.0
9	3780.0
10	4105.5
11	4552.0
12	4989.5
13	5602.0
14	6465.5
15	8064.0
16	11849.0
17	16249.5
18	19644.5
19	20311.0
20	20794.0
21	23701.0
22	29224.5
23	36141.5
24	45558.5
25	72522.5
26	116741.5
27	158254.5
28	164926.0
29	148025.0
30	152187.0
31	175789.0
32	205795.0
33	239608.5
34	263536.0
35	326534.5
36	540010.5
37	736614.0
38	822839.0
39	900274.0
40	1053636.0
41	1327216.5
42	1663515.5
43	1831694.5
44	2000268.0
45	2472009.0
46	3157072.0
47	3619368.0
48	4001867.0
49	4286575.0
50	3800298.5
51	3180074.5
52	2730672.0
53	2301507.0
54	1874590.0
55	1229670.0
56	744465.5
57	471641.5
58	255462.5
59	169162.5
60	138600.0
61	149822.5
62	210155.5
63	207385.5
64	138181.5
65	78021.5
66	42013.5
67	28363.5
68	10805.0
69	5806.5
70	3716.5
71	2630.5
72	1988.5
73	1583.0
74	1333.0
75	1151.0
76	996.0
77	871.0
78	775.0
79	673.0
80	611.0
81	581.5
82	520.5
83	453.0
84	404.5
85	336.0
86	273.5
87	241.0
88	198.5
89	172.0
90	136.0
91	111.0
92	93.0
93	74.0
94	69.0
95	60.0
96	47.5
97	43.5
98	42.0
99	42.0
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04770342368429765
2	0.051370540685293106
3	0.02004759299813865
4	0.013636318780667941
5	0.015809806666089405
6	0.020111458518942502
7	0.026716389476915223
8	0.0398623858720568
9	0.05175579398820668
10-11	0.06716386592665893
12-13	0.08837442945685486
14-15	0.1049331108588217
16-17	0.12315641615786962
18-19	0.14308245864867192
20-21	0.1601613350184766
22-23	0.1844601055062524
24-25	0.19539450072129927
26-27	0.21350552631441783
28-29	0.24473370580941173
30-31	0.2625964799426314
32-33	0.2707280028656253
34-35	0.2606310700443451
36-37	0.2286488653682477
38-39	0.206973731678656
40-41	0.18847642269357862
42-43	0.16345968014128207
44-45	0.15436296378291384
46-47	0.14615624435961866
48-49	0.13983252771099197
50-51	0.12939669559383327
52-53	0.12691521108388998
54-55	0.11989206397355653
56-57	0.11753522023808531
58-59	0.11789163104773262
60-61	0.11085303260172084
62-63	0.11364354382523113
64-65	0.11186354995508503
66-67	0.10935116277378505
68-69	0.1045725796929935
70-71	0.10548420849801625
72-73	0.09298407793422976
74-75	0.09056748903413556
76-77	0.08625141593464933
78-79	0.07951257340079758
80-81	0.07508834095156289
82-83	0.07661905327276493
84-85	0.08144605053868198
86-87	0.08669435422409542
88-89	0.09536667389583159
90-91	0.10269060700737026
92-93	0.11219111827146608
94-95	0.12512079596711073
96-97	0.13233450955081053
98-99	0.1522698228430198
100-101	0.15594518055637704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4.8539493E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.434957443857424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.98314830217022	13.580161498205939
2	6.393871048306971	1.973782550642603
3	2.090649687758207	0.9680726688168519
4	0.8302481803006303	0.5125938132312118
5	0.48387894072027504	0.3734325428998127
6	0.33494334153444205	0.31019017336125093
7	0.2637016176836015	0.2849156272775925
8	0.20000678896848964	0.24696770209689684
9	0.15957595257622748	0.22167432333693735
>10	0.9460676465271378	2.666236099112535
>50	0.10051252746801442	1.0621667845394103
>100	0.1099436284702651	3.797642003330663
>500	0.030014774606091792	3.2842805846960554
>1k	0.05079559419406535	17.928077839202867
>5k	0.01157060935893096	12.446424749909434
>10k+	0.011071359356331393	40.343381039339974
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	138298	0.2849185095526235	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	136267	0.28073428785092586	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	129318	0.2664181103004104	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	125950	0.25947943049178535	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	124485	0.25646126958928067	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	124429	0.2563458996162156	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	120454	0.24815669170668922	No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG	116632	0.24028269104500125	No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA	115566	0.23808654120058484	No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT	107851	0.22219226723278712	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	107845	0.22217990616424446	No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC	102091	0.21032564143181307	No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA	101370	0.20884025302860088	No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC	100171	0.20637009949815502	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	97144	0.2001339404183723	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	96727	0.19927484615465596	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	91626	0.18876587771528638	No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA	91520	0.18854749883769903	No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC	86906	0.1790418371283771	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	85101	0.17532321567512046	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	81190	0.16726585916338269	No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT	78833	0.16241001940419938	No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG	76917	0.15846271818290317	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	75741	0.1560399487485376	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	74665	0.15382319712321676	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG	68558	0.14124168952485763	No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG	66008	0.13598823539421806	No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA	65365	0.13466354088206073	No Hit
CTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAGAAT	65175	0.1342721070448758	No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA	64737	0.13336974904126006	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	64439	0.13275581597030692	No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC	63723	0.13128072845754693	No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT	62844	0.1294698319160441	No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC	62823	0.12942656817614473	No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT	62717	0.12920818929855735	No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG	60943	0.12555343336610458	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	57293	0.11803378333597346	No Hit
GCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTCTTCATTAATCA	57245	0.117934894787632	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	57050	0.11753316005999487	No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT	56850	0.11712112444190548	No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC	56016	0.11540293591447279	No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT	56012	0.11539469520211099	No Hit
GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA	55786	0.11492909495367	No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG	55639	0.1146262487743743	No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC	55527	0.11439550882824424	No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT	53782	0.11080049806041445	No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA	53708	0.11064804488172136	No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT	53573	0.11036992083951103	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	53437	0.11008973661921026	No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA	53273	0.10975186741237697	No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT	53175	0.10954996995951319	No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG	53041	0.1092739060953933	No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA	52928	0.10904110597117278	No Hit
GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC	51562	0.10622690269962234	No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA	51218	0.10551820143650861	No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC	50667	0.10438304330867237	No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT	50147	0.10331175070164	No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG	49945	0.10289559472736973	No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG	49679	0.10234758735531085	No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT	49648	0.10228372183450701	No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA	48856	0.10065206078687307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0027626988192892745	0.0	0.0
2	0.002843045764816703	0.0	0.0
3	0.0030552441081327323	0.0	0.0
4	0.0033004053008959117	0.0	0.0
5	0.003669177179085904	0.0	2.0601780904468862E-6
6	0.0038319312482312084	0.0	4.1203561808937725E-6
7	0.004011166742100087	0.0	4.1203561808937725E-6
8	0.004272809359586842	0.0	6.180534271340659E-6
9	0.005243153240187325	0.0	8.240712361787545E-6
10-11	0.006226888278375713	2.0601780904468862E-6	9.270801407010989E-6
12-13	0.006937649719579889	3.0902671356703294E-6	1.030089045223443E-5
14-15	0.008162425594350563	4.1203561808937725E-6	1.2361068542681316E-5
16-17	0.00929243327696068	4.1203561808937725E-6	2.2661958994915748E-5
18-19	0.010168008965400606	5.150445226117216E-6	2.2661958994915748E-5
20-21	0.010961177530222658	8.240712361787545E-6	2.2661958994915748E-5
22-23	0.011725503601778453	9.270801407010989E-6	2.4722137085362635E-5
24-25	0.012710268729012065	1.1330979497457874E-5	2.6782315175809522E-5
26-27	0.014291455413430049	1.4421246633128203E-5	2.8842493266256406E-5
28-29	0.016898610786890585	2.060178090446886E-5	3.090267135670329E-5
30-31	0.025076487716919497	2.060178090446886E-5	3.090267135670329E-5
32-33	0.029811807057811668	2.060178090446886E-5	3.090267135670329E-5
34-35	0.03583576779427836	2.060178090446886E-5	3.090267135670329E-5
36-37	0.04327610096792729	2.2661958994915748E-5	3.296284944715018E-5
38-39	0.051911337434035414	2.4722137085362635E-5	3.296284944715018E-5
40-41	0.06205771452948633	2.6782315175809522E-5	3.296284944715018E-5
42-43	0.07457226633990593	2.6782315175809522E-5	3.502302753759707E-5
44-45	0.08900175368539592	2.6782315175809522E-5	3.7083205628043954E-5
46-47	0.10649884620756134	2.8842493266256406E-5	4.326373989938461E-5
48-49	0.1270789952420805	2.8842493266256406E-5	4.4293828944608055E-5
50-51	0.15233265827477843	3.193276040192673E-5	7.004605507519413E-5
52-53	0.18100518684857297	3.296284944715018E-5	7.004605507519413E-5
54-55	0.21554407253491503	3.296284944715018E-5	7.004605507519413E-5
56-57	0.2522018513872817	3.296284944715018E-5	7.004605507519413E-5
58-59	0.29102075705652714	3.502302753759707E-5	7.313632221086446E-5
60-61	0.33629935112836884	4.017347276371428E-5	7.416641125608791E-5
62-63	0.3881087097469271	4.5323917989831496E-5	7.416641125608791E-5
64-65	0.4480825541379264	4.944427417072527E-5	7.622658934653479E-5
66-67	0.5182429490971403	5.2534541306395604E-5	7.828676743698168E-5
68-69	0.5983787263702981	5.3564630351619044E-5	8.240712361787544E-5
70-71	0.6870745436092627	5.3564630351619044E-5	8.240712361787544E-5
72-73	0.7950052135896846	5.3564630351619044E-5	8.240712361787544E-5
74-75	0.9224931541827188	5.3564630351619044E-5	8.343721266309889E-5
76-77	1.0537738826402658	5.3564630351619044E-5	8.549739075354578E-5
78-79	1.2069069201031828	5.4594719396842484E-5	8.755756884399266E-5
80-81	1.3864905840693473	5.6654897487289364E-5	8.858765788921611E-5
82-83	1.5807694983546696	6.077525366818314E-5	9.167792502488644E-5
84-85	1.7968430366588295	6.180534271340659E-5	9.476819216055677E-5
86-87	2.0403550568606064	6.386552080385347E-5	9.476819216055677E-5
88-89	2.3109985924245233	6.592569889430036E-5	9.682837025100365E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGC	14475	0.0	78.62435	9
AAGGGGC	41905	0.0	77.21605	9
AACGGGA	38090	0.0	76.784645	2
GAGAGCG	18845	0.0	74.992874	7
ACGGGAG	22255	0.0	74.77342	3
GGAGAGC	20785	0.0	72.85609	6
GATTGGG	15620	0.0	72.686966	1
GTCCAAT	14110	0.0	70.94568	1
AAGCGGC	12690	0.0	70.7518	9
GAGGGGC	55795	0.0	69.8388	9
GGGAGAG	73385	0.0	69.792244	5
ATCCGGA	14090	0.0	69.77554	2
TCCAATC	14560	0.0	69.05557	2
AGAGCGG	20190	0.0	68.75592	8
GATCCGG	14855	0.0	67.51546	1
ATTGGGA	16890	0.0	65.65644	2
TTGGGAG	11325	0.0	62.614563	3
CGGGAAA	55140	0.0	59.183567	4
TGGGAGA	16075	0.0	55.130066	4
CGGGAGA	74175	0.0	54.98984	4
>>END_MODULE