FastQCFastQC Report
Thu 14 Aug 2014
brugia_2_CGATGT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebrugia_2_CGATGT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48539493
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1382980.2849185095526235No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT1362670.28073428785092586No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC1293180.2664181103004104No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT1259500.25947943049178535No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG1244850.25646126958928067No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG1244290.2563458996162156No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC1204540.24815669170668922No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG1166320.24028269104500125No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA1155660.23808654120058484No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT1078510.22219226723278712No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG1078450.22217990616424446No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC1020910.21032564143181307No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA1013700.20884025302860088No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC1001710.20637009949815502No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA971440.2001339404183723No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG967270.19927484615465596No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA916260.18876587771528638No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA915200.18854749883769903No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC869060.1790418371283771No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC851010.17532321567512046No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC811900.16726585916338269No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT788330.16241001940419938No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG769170.15846271818290317No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC757410.1560399487485376No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG746650.15382319712321676No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG685580.14124168952485763No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG660080.13598823539421806No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA653650.13466354088206073No Hit
CTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAGAAT651750.1342721070448758No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA647370.13336974904126006No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT644390.13275581597030692No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC637230.13128072845754693No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT628440.1294698319160441No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC628230.12942656817614473No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT627170.12920818929855735No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG609430.12555343336610458No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT572930.11803378333597346No Hit
GCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTCTTCATTAATCA572450.117934894787632No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC570500.11753316005999487No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT568500.11712112444190548No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC560160.11540293591447279No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT560120.11539469520211099No Hit
GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA557860.11492909495367No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG556390.1146262487743743No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC555270.11439550882824424No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT537820.11080049806041445No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA537080.11064804488172136No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT535730.11036992083951103No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG534370.11008973661921026No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA532730.10975186741237697No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT531750.10954996995951319No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG530410.1092739060953933No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA529280.10904110597117278No Hit
GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC515620.10622690269962234No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA512180.10551820143650861No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC506670.10438304330867237No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT501470.10331175070164No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG499450.10289559472736973No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG496790.10234758735531085No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT496480.10228372183450701No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA488560.10065206078687307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC144750.078.624359
AAGGGGC419050.077.216059
AACGGGA380900.076.7846452
GAGAGCG188450.074.9928747
ACGGGAG222550.074.773423
GGAGAGC207850.072.856096
GATTGGG156200.072.6869661
GTCCAAT141100.070.945681
AAGCGGC126900.070.75189
GAGGGGC557950.069.83889
GGGAGAG733850.069.7922445
ATCCGGA140900.069.775542
TCCAATC145600.069.055572
AGAGCGG201900.068.755928
GATCCGG148550.067.515461
ATTGGGA168900.065.656442
TTGGGAG113250.062.6145633
CGGGAAA551400.059.1835674
TGGGAGA160750.055.1300664
CGGGAGA741750.054.989844
Produced by FastQC (version 0.11.2)