##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	brugia_2_CGATGT_L002_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48539493
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73225200353865	33.0	33.0	33.0	33.0	33.0
2	32.69086879420022	33.0	33.0	33.0	33.0	33.0
3	32.69121391523393	33.0	33.0	33.0	33.0	33.0
4	36.5695034762724	37.0	37.0	37.0	37.0	37.0
5	36.48248771366442	37.0	37.0	37.0	37.0	37.0
6	36.432601840320004	37.0	37.0	37.0	37.0	37.0
7	36.41989183941414	37.0	37.0	37.0	37.0	37.0
8	36.411787366629476	37.0	37.0	37.0	37.0	37.0
9	36.421940089073445	37.0	37.0	37.0	37.0	37.0
10-11	36.40520065794672	37.0	37.0	37.0	37.0	37.0
12-13	36.40976031620272	37.0	37.0	37.0	37.0	37.0
14-15	38.424373715646354	40.0	37.0	40.0	37.0	40.0
16-17	38.433605105846496	40.0	37.0	40.0	37.0	40.0
18-19	38.405516081513255	40.0	37.0	40.0	37.0	40.0
20-21	38.416814747117364	40.0	37.0	40.0	37.0	40.0
22-23	38.35858127937183	40.0	37.0	40.0	37.0	40.0
24-25	38.34157254176512	40.0	37.0	40.0	37.0	40.0
26-27	38.2869798413428	40.0	37.0	40.0	37.0	40.0
28-29	38.17756149616149	40.0	37.0	40.0	37.0	40.0
30-31	38.11792823011151	40.0	37.0	40.0	35.0	40.0
32-33	38.08475379007358	40.0	37.0	40.0	33.0	40.0
34-35	38.029140662841286	40.0	37.0	40.0	33.0	40.0
36-37	37.84692356593011	40.0	37.0	40.0	33.0	40.0
38-39	37.6956906513218	40.0	37.0	40.0	33.0	40.0
40-41	37.61111358332482	40.0	37.0	40.0	33.0	40.0
42-43	37.4563246880226	40.0	37.0	40.0	33.0	40.0
44-45	37.50656837309776	40.0	37.0	40.0	33.0	40.0
46-47	37.329040756564964	40.0	37.0	40.0	33.0	40.0
48-49	37.272103624980176	40.0	37.0	40.0	33.0	40.0
50-51	37.04998517392838	38.5	37.0	40.0	33.0	40.0
52-53	36.776893765660056	37.0	37.0	40.0	33.0	40.0
54-55	36.58137074072859	37.0	37.0	40.0	33.0	40.0
56-57	36.370061735090644	37.0	37.0	40.0	33.0	40.0
58-59	36.02072329020824	37.0	37.0	40.0	33.0	40.0
60-61	35.84653428497904	37.0	37.0	37.0	33.0	40.0
62-63	35.79591506034066	37.0	37.0	37.0	33.0	40.0
64-65	35.382191322023075	37.0	33.0	37.0	33.0	40.0
66-67	35.29483335353338	37.0	33.0	37.0	33.0	40.0
68-69	35.0207779364321	37.0	33.0	37.0	33.0	37.0
70-71	34.694547303986056	37.0	33.0	37.0	27.0	37.0
72-73	34.21820061037721	37.0	33.0	37.0	27.0	37.0
74-75	33.34341594791688	37.0	33.0	37.0	27.0	37.0
76-77	33.087307926763884	37.0	33.0	37.0	24.5	37.0
78-79	33.0542058298796	37.0	33.0	37.0	24.5	37.0
80-81	33.08751833275226	37.0	33.0	37.0	24.5	37.0
82-83	33.16444102537288	37.0	33.0	37.0	27.0	37.0
84-85	33.148254473939396	37.0	33.0	37.0	27.0	37.0
86-87	33.12883127971691	37.0	33.0	37.0	27.0	37.0
88-89	33.0768825912541	37.0	33.0	37.0	27.0	37.0
90-91	33.05589323934636	37.0	33.0	37.0	24.5	37.0
92-93	32.985399167642726	37.0	33.0	37.0	22.0	37.0
94-95	32.9748814125438	37.0	33.0	37.0	22.0	37.0
96-97	32.930991646328074	37.0	33.0	37.0	22.0	37.0
98-99	32.83621148453281	37.0	33.0	37.0	22.0	37.0
100-101	32.43687892454913	35.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.06721657359998545
2201	2	0.10080875508554499
2201	3	0.10144815571134558
2201	4	0.1412335684085093
2201	5	0.1433819646679595
2201	6	0.17485609461483165
2201	7	0.19562420730794372
2201	8	0.18305403014723254
2201	9	0.16635964381133306
2201	10-11	0.17304019361885992
2201	12-13	0.1579963434056424
2201	14-15	0.3743877960477775
2201	16-17	0.3433042682888683
2201	18-19	0.38234343127700754
2201	20-21	0.3597435753685403
2201	22-23	0.3530186672371869
2201	24-25	0.10432346754821964
2201	26-27	0.18365254792288255
2201	28-29	0.17944816825903587
2201	30-31	0.08512701695971003
2201	32-33	0.21359930308566533
2201	34-35	0.2225634433059085
2201	36-37	0.37949355780610716
2201	38-39	0.4226625260775734
2201	40-41	0.47816746691931655
2201	42-43	0.47977680529976396
2201	44-45	0.423187688610696
2201	46-47	0.46559652727677303
2201	48-49	0.4422701483319429
2201	50-51	0.40026958001669755
2201	52-53	0.40938419237839696
2201	54-55	0.4447879768303622
2201	56-57	0.42556422484695133
2201	58-59	0.3956343382578211
2201	60-61	0.4180544429559987
2201	62-63	0.35370046585949666
2201	64-65	0.34943116382714834
2201	66-67	0.399922808424634
2201	68-69	0.38711055583349463
2201	70-71	0.434187037361383
2201	72-73	0.4087594964524044
2201	74-75	0.417392324835248
2201	76-77	0.32030044285342285
2201	78-79	0.3593842184974534
2201	80-81	0.24517742864422587
2201	82-83	0.35294616965062175
2201	84-85	0.37578507864202493
2201	86-87	0.4057432651264534
2201	88-89	0.4485655269180313
2201	90-91	0.4283424357547574
2201	92-93	0.4731648026599231
2201	94-95	0.5092086797215885
2201	96-97	0.46126865671299555
2201	98-99	0.47509391508610577
2201	100-101	0.53481662360673
1101	1	0.07917924693160927
1101	2	0.09908418332447155
1101	3	0.1011410829992343
1101	4	0.13546599316479302
1101	5	0.14977054194567074
1101	6	0.1666144891642105
1101	7	0.15071843254609973
1101	8	0.15254776088836763
1101	9	0.16366978514606956
1101	10-11	0.16522209171542102
1101	12-13	0.14006190465617863
1101	14-15	-0.032485389772745066
1101	16-17	0.11961021646048664
1101	18-19	0.17930256306975423
1101	20-21	0.19424548405242348
1101	22-23	0.2454484367677594
1101	24-25	0.27693555683877946
1101	26-27	0.07897782965062561
1101	28-29	0.11661483649082527
1101	30-31	0.2811994644237217
1101	32-33	0.2997232455585035
1101	34-35	0.3543604727413481
1101	36-37	0.28245720251527473
1101	38-39	0.2875422641246601
1101	40-41	0.29301996219206927
1101	42-43	0.2586634001902439
1101	44-45	0.2186256352682605
1101	46-47	0.15903390492357516
1101	48-49	0.34513604595902336
1101	50-51	0.29167235219766496
1101	52-53	0.2843165856555103
1101	54-55	0.2587452585778962
1101	56-57	0.33077360505697584
1101	58-59	0.32335289754981744
1101	60-61	0.3133688703198061
1101	62-63	0.27748842423071807
1101	64-65	0.34702076102006885
1101	66-67	0.3468222825899474
1101	68-69	0.2284183766674346
1101	70-71	0.2875169433504752
1101	72-73	0.12873195882349364
1101	74-75	0.33770480439048356
1101	76-77	0.30503744614756556
1101	78-79	0.27603651492760406
1101	80-81	0.30987417726471733
1101	82-83	0.30633586755089937
1101	84-85	0.14276459355461668
1101	86-87	-0.1108686263519374
1101	88-89	0.25530129344875974
1101	90-91	0.31150774685032445
1101	92-93	0.3386509061710683
1101	94-95	0.39736758144169215
1101	96-97	0.030755098527279756
1101	98-99	0.12324914526820407
1101	100-101	0.11914233309788358
2202	1	0.07375894832519236
2202	2	0.0701047068941989
2202	3	0.0615769136522033
2202	4	0.09523514048699155
2202	5	0.09676103523745638
2202	6	0.1037158336447419
2202	7	0.14264019033754494
2202	8	0.16235157985190085
2202	9	0.15153300618712962
2202	10-11	0.1369262325994569
2202	12-13	0.11957087071606054
2202	14-15	0.19231078260840206
2202	16-17	0.14866957303008377
2202	18-19	0.09961054507866862
2202	20-21	0.0719998746722581
2202	22-23	0.14572474996288065
2202	24-25	0.21201207020202162
2202	26-27	0.23816320045999362
2202	28-29	0.1834018924190488
2202	30-31	0.27806346464939224
2202	32-33	0.21238015150687062
2202	34-35	0.2807202020535371
2202	36-37	0.21095737427662442
2202	38-39	0.20897832717973586
2202	40-41	0.14714892929509205
2202	42-43	0.2152208458366971
2202	44-45	0.268829859184315
2202	46-47	0.2308314269601368
2202	48-49	0.19987826014892107
2202	50-51	0.23589827507843353
2202	52-53	0.23859917861602753
2202	54-55	0.3071467340601757
2202	56-57	0.31106927827730146
2202	58-59	0.23318269281605097
2202	60-61	0.30289059245092176
2202	62-63	0.31413487123330697
2202	64-65	0.30227964241127125
2202	66-67	0.31195861517343104
2202	68-69	0.27463828532952306
2202	70-71	0.29042543436238333
2202	72-73	0.270658783412415
2202	74-75	0.22848310287767504
2202	76-77	0.14789532853207987
2202	78-79	0.16709850023428885
2202	80-81	0.18581772312555245
2202	82-83	0.18050184780439338
2202	84-85	0.2092803260914664
2202	86-87	0.19114014048301442
2202	88-89	0.23391513026277266
2202	90-91	0.2543622175197484
2202	92-93	0.23782943291911351
2202	94-95	0.2571787294840391
2202	96-97	0.2957124902825541
2202	98-99	0.36789571757542205
2202	100-101	0.42385857695463613
1102	1	0.05732570888415722
1102	2	0.05388507539480969
1102	3	0.0470854717955973
1102	4	0.07124104158199174
1102	5	0.10245687453782892
1102	6	0.1091675165588768
1102	7	0.11933761651835084
1102	8	0.11224565922773166
1102	9	0.10801361966039735
1102	10-11	0.12198892176988352
1102	12-13	0.10641540733908528
1102	14-15	0.2501880293449261
1102	16-17	0.2305037117482982
1102	18-19	0.2366178985077596
1102	20-21	0.24602717033281607
1102	22-23	0.2114916209510227
1102	24-25	0.179106914767047
1102	26-27	0.22012723676137114
1102	28-29	0.25021656609164467
1102	30-31	0.23295392832335438
1102	32-33	0.28433997313465653
1102	34-35	0.20834868626445768
1102	36-37	0.2263933675723706
1102	38-39	0.2847920231990102
1102	40-41	0.24653321891067748
1102	42-43	0.25294509380863417
1102	44-45	0.25284239042075995
1102	46-47	0.27442484183585236
1102	48-49	0.2823141036493837
1102	50-51	0.26175834892598715
1102	52-53	0.2470441246667221
1102	54-55	0.3043781875361091
1102	56-57	0.2529285532073118
1102	58-59	0.2515092538368151
1102	60-61	0.24136570326081852
1102	62-63	0.2320539874675518
1102	64-65	0.19791347460624564
1102	66-67	0.23954297463593832
1102	68-69	0.19560886779596132
1102	70-71	0.20184011340288777
1102	72-73	0.18898968217784073
1102	74-75	0.21247341068899317
1102	76-77	0.22333280065302574
1102	78-79	0.23804467766009907
1102	80-81	0.2416017194225475
1102	82-83	0.24137126554096966
1102	84-85	0.2124077699330158
1102	86-87	0.2181510236422355
1102	88-89	0.2933157175647949
1102	90-91	0.27160227528960235
1102	92-93	0.32867252951260895
1102	94-95	0.3135816005757519
1102	96-97	0.28562635345993215
1102	98-99	0.2806759882921881
1102	100-101	0.3004390679415465
2203	1	-0.004327037264161504
2203	2	0.02593841207973213
2203	3	0.0443055328398998
2203	4	0.06549661499294501
2203	5	0.03891889105199198
2203	6	0.07405074920250598
2203	7	0.09613588055794509
2203	8	0.10477469562733432
2203	9	0.08428878431595166
2203	10-11	0.056453922582093696
2203	12-13	0.08734979145984312
2203	14-15	0.1596991541036985
2203	16-17	0.14084096348912567
2203	18-19	0.1726381571968858
2203	20-21	0.1407590882662717
2203	22-23	0.15832492374187268
2203	24-25	0.18438011724688863
2203	26-27	0.16717879739747588
2203	28-29	0.23862529723668757
2203	30-31	0.1794998091122224
2203	32-33	0.19357163589157977
2203	34-35	0.21490370631799038
2203	36-37	0.22658935489884158
2203	38-39	0.19815617419630627
2203	40-41	0.16714304740401786
2203	42-43	0.13132281764215037
2203	44-45	0.1280372505060754
2203	46-47	0.10329836411788307
2203	48-49	0.11954334543901268
2203	50-51	0.1263922860282065
2203	52-53	0.16234580186460335
2203	54-55	0.15703143331710123
2203	56-57	0.19100135349501812
2203	58-59	0.17990151422095124
2203	60-61	0.15038174340165256
2203	62-63	0.12400958214458768
2203	64-65	0.17224657394547904
2203	66-67	0.1678265718664349
2203	68-69	0.16953840967654088
2203	70-71	0.12802886882523268
2203	72-73	0.13072632253880556
2203	74-75	0.18668184312120673
2203	76-77	0.12936407474826694
2203	78-79	0.15691580219626644
2203	80-81	0.19302055404004648
2203	82-83	0.1753213188400906
2203	84-85	0.12219965755760143
2203	86-87	0.13245681842172274
2203	88-89	0.1784388422922376
2203	90-91	0.09282998857727875
2203	92-93	0.1776902063218131
2203	94-95	0.1564541348529218
2203	96-97	0.15446583489162435
2203	98-99	0.17248986554680812
2203	100-101	0.1990502522266766
1103	1	0.02241405208535241
1103	2	0.03656726965554924
1103	3	0.030495155489127512
1103	4	0.048946644237013004
1103	5	0.056790848463492694
1103	6	0.06820506077416155
1103	7	0.06747791425610217
1103	8	0.07267629769130224
1103	9	0.04099238978278663
1103	10-11	0.05530362339117545
1103	12-13	0.04942795345078821
1103	14-15	0.12226421070145221
1103	16-17	0.08426424814783928
1103	18-19	0.09047880963816368
1103	20-21	0.1291178936866828
1103	22-23	0.1383353656223676
1103	24-25	0.12991095681525167
1103	26-27	0.11197963417505008
1103	28-29	0.1648240895308959
1103	30-31	0.16860980934293224
1103	32-33	0.12652547440386996
1103	34-35	0.11439253408941141
1103	36-37	0.11331104652042256
1103	38-39	0.12731411774713308
1103	40-41	0.13446839667603427
1103	42-43	0.1442283115941052
1103	44-45	0.07122810975334204
1103	46-47	0.10210005673184241
1103	48-49	0.1379514541023923
1103	50-51	0.13174169730259422
1103	52-53	0.08760824199639927
1103	54-55	0.14513109525129408
1103	56-57	0.10301087613950699
1103	58-59	0.1468694175166192
1103	60-61	0.1396623443966547
1103	62-63	0.04176168524603696
1103	64-65	0.10091470623867593
1103	66-67	0.1025620738165145
1103	68-69	0.12273486238448328
1103	70-71	0.1352390179712657
1103	72-73	0.059101343833155795
1103	74-75	0.08181822739333455
1103	76-77	0.14650044044352484
1103	78-79	0.1057611196107402
1103	80-81	0.0689381005734262
1103	82-83	0.12501467790598042
1103	84-85	0.12680667905854648
1103	86-87	0.11216433104446111
1103	88-89	0.13734042127720159
1103	90-91	0.14525337874695055
1103	92-93	0.15427363000863892
1103	94-95	0.13484297522698796
1103	96-97	0.17019501286652883
1103	98-99	0.15645652417057931
1103	100-101	0.21654193078899198
2204	1	0.021485194797065787
2204	2	0.026424636427371695
2204	3	0.03726610979654055
2204	4	0.03866765208853451
2204	5	0.06409323117056687
2204	6	0.043979661274946125
2204	7	0.04331166592411506
2204	8	0.030001247126627106
2204	9	0.053134338460083086
2204	10-11	0.06347518321922507
2204	12-13	0.054758719324453864
2204	14-15	0.11547336390506757
2204	16-17	0.08963916648595216
2204	18-19	0.09890765216267994
2204	20-21	0.061303871000887966
2204	22-23	0.021255206380317304
2204	24-25	0.058905496552931425
2204	26-27	0.05301155991766393
2204	28-29	0.08030354405878626
2204	30-31	0.08451479094203052
2204	32-33	0.12370724177691983
2204	34-35	0.13657282587721653
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1213	42-43	-0.5781500341659154
1213	44-45	-0.6317686481855915
1213	46-47	-0.5474467017187123
1213	48-49	-0.6993740945645186
1213	50-51	-0.5972042147338925
1213	52-53	-0.6152181686351028
1213	54-55	-0.6280880460958898
1213	56-57	-0.6044293918724435
1213	58-59	-0.6406780958425955
1213	60-61	-0.6556723132886901
1213	62-63	-0.6221466960613355
1213	64-65	-0.5504323333587777
1213	66-67	-0.5590436392970588
1213	68-69	-0.6241457750350534
1213	70-71	-0.6862096514566005
1213	72-73	-0.6719773580366422
1213	74-75	-0.43224246325449656
1213	76-77	-0.4997418216131564
1213	78-79	-0.5791742416624714
1213	80-81	-0.5354686098361015
1213	82-83	-0.5250374631243844
1213	84-85	-0.41427068082587937
1213	86-87	-0.4951592370482629
1213	88-89	-0.43434808910080847
1213	90-91	-0.4920863931645627
1213	92-93	-0.6844503533143893
1213	94-95	-0.6092821201132637
1213	96-97	-0.6766233676328568
1213	98-99	-0.6169022669539999
1213	100-101	-0.6650835499588368
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	44.0
11	114.0
12	268.0
13	922.0
14	2981.0
15	7083.0
16	12956.0
17	21003.0
18	33354.0
19	50833.0
20	73681.0
21	103602.0
22	141997.0
23	190831.0
24	251924.0
25	329606.0
26	435070.0
27	579688.0
28	826556.0
29	1511479.0
30	1379124.0
31	929271.0
32	1199811.0
33	1642269.0
34	2269260.0
35	3138678.0
36	5497433.0
37	1.7673345E7
38	1.0230467E7
39	5832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.28722347046683	11.045699874666795	13.198244045178805	36.468832609687574
2	20.814461329458055	23.124872771126803	38.838582430187316	17.22208346922783
3	21.71479667013543	21.17796124989073	31.01258988454495	26.094652195428893
4	24.940145130893722	31.056469831689427	18.94486001326796	25.058525024148892
5	28.821844054034795	31.92483114255754	20.848206089838683	18.40511871356898
6	22.207279750532212	34.19480710274415	24.88508687142653	18.712826275297108
7	14.013630097042833	20.777722173571117	42.369227053937294	22.83942067544875
8	18.19389007627253	23.80871798557929	27.95488613776827	30.04250580037991
9	29.209849802098265	19.709091316631593	27.95943295081389	23.121625930456258
10-11	25.277747544664305	34.125506832137695	19.657215002225094	20.939530620972903
12-13	21.320295825916435	24.64530583374655	28.095844552805694	25.938553787531323
14-15	22.16974536590236	27.672088581559763	26.54617241263727	23.611993639900604
16-17	25.479575981562064	24.910361136240134	25.58834102366912	24.021721858528682
18-19	21.67924168470404	24.81798584093163	27.805212139319213	25.697560335045118
20-21	25.473926973238058	24.695665857078485	27.85586367785094	21.974543491832517
22-23	22.220966634668756	31.40713761212574	24.383256652758696	21.988639100446807
24-25	21.75958657005338	25.64720958251459	27.68492864150847	24.908275205923555
26-27	22.708923844754622	24.506148014360182	25.482432418484468	27.302495722400728
28-29	24.846398369356365	27.76209909314628	24.71546133456516	22.676041202932193
30-31	21.638213365288376	25.150293998511085	27.933832125588825	25.277660510611717
32-33	21.049761998000456	27.91265258218284	25.10227530438973	25.935310115426972
34-35	23.563915932820017	26.320847482337033	25.64327958166927	24.471957003173674
36-37	21.670904127357446	28.891812179370568	27.877050735599962	21.56023295767202
38-39	24.626078191628412	24.78611179560528	28.820583272264503	21.7672267405018
40-41	21.848493892382372	28.90030228777198	28.7052666874244	20.545937132421248
42-43	21.49978158498605	25.154919013389815	28.959025916112875	24.38627348551126
44-45	24.902387216941058	26.102885953093907	25.715791881056525	23.27893494890851
46-47	22.400779483343104	27.549856424679607	28.24262452236929	21.806739569607995
48-49	24.84045604010904	24.862974209495594	28.112257462653705	22.18431228774166
50-51	21.55289214259849	24.872575810942347	25.396162405243732	28.178369641215433
52-53	25.474682080658102	24.86389424383062	28.25783128166495	21.403592393846328
54-55	22.52946255779495	24.62233069655925	27.841977281631458	25.00622946401434
56-57	21.297686687684607	24.905134973606028	28.616205073436085	25.180973265273288
58-59	25.255206905666327	24.931856057894677	28.669495603197344	21.143441433241655
60-61	22.05293816127318	25.712399659233725	28.36302393309429	23.87163824639881
62-63	24.009749637604084	26.096165945411553	28.443512669381416	21.450571747602947
64-65	21.08206271233854	32.36274390780026	24.984642114894072	21.570551264967133
66-67	22.60594220116446	32.11045846494591	23.85161263879149	21.43198669509814
68-69	21.2609307764985	31.567673265599517	25.86345412387987	21.307941834022117
70-71	21.49106196723383	30.896740697691893	25.425511699812215	22.186685635262066
72-73	21.90043862437697	31.243319362462852	24.644324619041157	22.211917394119016
74-75	22.3396245276227	30.306292012900876	25.95006493127619	21.404018528200233
76-77	23.043387220918248	28.329214358896003	25.90672729152597	22.720671128659774
78-79	23.37746236838794	28.29962802881726	25.0754080553871	23.2475015474077
80-81	24.431156123453462	27.622953168134785	25.436588145944803	22.509302562466953
82-83	23.520337561825784	27.750968024357114	25.8637848916354	22.864909522181705
84-85	23.11198458365373	28.615181977671888	24.857876630393324	23.414956808281058
86-87	22.258456622048875	29.22443542679478	25.672727310188947	22.844380640967398
88-89	22.896872342423524	28.04572814675082	26.904325124787505	22.153074386038156
90-91	23.58200859255568	27.132491191154294	26.718857764519342	22.566642451770683
92-93	22.784321528291887	26.748488083852255	27.69022237598194	22.776968011873922
94-95	23.162773313430172	27.18085718461839	25.703276281426046	23.953093220525393
96-97	22.069002957590428	29.44791834225772	25.289479551956035	23.193599148195826
98-99	23.50356695555308	28.36170086493886	25.578378303538194	22.55635387596987
100-101	22.998527360654162	27.013372125696613	26.016966696736894	23.97113381691233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2377.0
1	1984.5
2	1439.0
3	1371.5
4	1464.5
5	1504.0
6	1592.5
7	1703.0
8	1853.5
9	2027.0
10	2212.0
11	2365.5
12	2584.0
13	3042.5
14	3614.0
15	4827.5
16	8490.0
17	12983.0
18	16941.0
19	17291.5
20	16489.0
21	19562.0
22	25228.0
23	31842.0
24	41864.0
25	69381.5
26	113514.5
27	154959.5
28	157503.5
29	135940.0
30	139965.0
31	164112.0
32	192163.0
33	225731.0
34	252415.5
35	318223.5
36	579921.5
37	817463.0
38	918167.5
39	1023189.5
40	1230550.0
41	1585401.0
42	1976169.0
43	2122499.5
44	2220644.5
45	2643356.5
46	3230910.0
47	3586478.0
48	3880003.0
49	4098444.5
50	3603845.5
51	2996827.0
52	2549014.0
53	2124494.0
54	1743579.0
55	1123287.5
56	659881.5
57	412339.5
58	205609.0
59	135371.5
60	112564.0
61	131543.5
62	211764.5
63	209910.5
64	128514.0
65	67656.5
66	35424.5
67	21486.0
68	2058.0
69	797.0
70	382.0
71	182.0
72	91.5
73	68.5
74	59.0
75	50.0
76	37.0
77	25.5
78	16.0
79	11.5
80	11.0
81	6.5
82	3.5
83	2.5
84	3.0
85	1.5
86	0.5
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02153710175753175
2	0.0
3	0.02693682853259304
4	0.0
5	0.0028018422030077652
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.001868581528035326
24-25	0.0
26-27	0.0
28-29	2.7194350793898896E-4
30-31	9.57982812057802E-5
32-33	8.34372126630989E-4
34-35	7.210623316564102E-6
36-37	4.182161523607179E-4
38-39	0.0
40-41	3.079966245218095E-4
42-43	3.8113294673267394E-5
44-45	0.0
46-47	3.3374885065239556E-4
48-49	0.0018778523294423366
50-51	0.06759032279138144
52-53	4.449984675365274E-4
54-55	8.240712361787545E-6
56-57	6.180534271340659E-6
58-59	0.028264613311886056
60-61	0.018271719484173436
62-63	0.0022116011800947326
64-65	0.006902626692042293
66-67	1.8438593909499634E-4
68-69	0.0018799125075327835
70-71	6.520463656264395E-4
72-73	0.003780426795970036
74-75	0.004163619920793157
76-77	0.0027163448122542195
78-79	5.129843445212746E-4
80-81	0.015594518055637706
82-83	0.010984869578262797
84-85	0.005563510933251816
86-87	0.010389478110123647
88-89	0.002840985586726256
90-91	0.0010300890452234431
92-93	0.010074270862285272
94-95	0.017537265994929118
96-97	0.005182377986519143
98-99	0.007487717269729207
100-101	0.012125178151325149
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4.8539493E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.236572291538202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19692352474355	6.60547758170511
2	10.548293476343682	1.7376356354053113
3	3.3503123235179233	0.8278526895566012
4	1.6461187117169336	0.5423350307804103
5	0.905558508932389	0.3729349061519583
6	0.6371630303387843	0.31488236165285677
7	0.428601079254309	0.24711426414565874
8	0.2942007552837985	0.1938564631296117
9	0.2027148720363397	0.15027081282875052
>10	1.2738045508663332	1.8862694099568917
>50	0.14689315775550119	0.8501938049030403
>100	0.1789409126689731	3.350707049733325
>500	0.050166848694837715	2.930884060085695
>1k	0.09572184654497733	18.378546879783052
>5k	0.022812321413153182	13.161997378902013
>10k+	0.021774079888682164	48.449041671279794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	2669569	5.499787564736203	TruSeq Adapter, Index 2 (100% over 50bp)
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	141174	0.2908435817407487	No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	139483	0.28735982058980303	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	130807	0.2694857154770859	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	130792	0.26945481280572914	No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG	122616	0.2526107967382354	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	120399	0.24804338191171468	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	119033	0.24522917864016422	No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC	114645	0.23618911717928326	No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT	112868	0.23252818071255915	No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA	111379	0.22946057553588375	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	101739	0.20960045874397576	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	100125	0.20627533130599449	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	97663	0.20120317284731426	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	96506	0.1988195467966672	No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC	95730	0.1972208485984804	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	95414	0.1965698323218992	No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA	92740	0.19106091610804424	No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA	91183	0.18785321882121842	No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA	89708	0.18481445613780925	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	89226	0.18382145029821387	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	88832	0.1830097401305778	No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT	86795	0.17881315736033748	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	84435	0.17395113706688284	No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG	83951	0.17295401087110654	No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC	74700	0.1538953033563824	No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA	74542	0.15356979521809178	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	73542	0.1515096171276449	No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG	73226	0.1508586008510637	No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC	71027	0.14632826923017098	No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG	70488	0.1452178332394201	No Hit
CTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAGAAT	70394	0.1450241764989181	No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA	69080	0.1423171024880709	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG	67306	0.13866234655561813	No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT	66149	0.13627872050497108	No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC	64582	0.1330504214372408	No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA	63688	0.1312086222243813	No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG	63541	0.1309057760450856	No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT	63411	0.1306379528933275	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	63127	0.1300528623156406	No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT	60014	0.12363952792007944	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	59881	0.12336552423404998	No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC	58687	0.12090567159405641	No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT	58184	0.11986940201456162	No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA	58089	0.11967368509596917	No Hit
GCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTCTTCATTAATCA	57779	0.11903502988793065	No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT	57092	0.11761968753979363	No Hit
GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA	57058	0.11754964148471844	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	56350	0.11609103539668203	No Hit
TTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCCACTTAT	55161	0.1136414836471407	No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC	54948	0.11320266571387551	No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG	54938	0.11318206393297105	No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC	54893	0.11308935591890093	No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA	54791	0.11287921775367535	No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA	53902	0.11104771943126807	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	53708	0.11064804488172136	No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA	52594	0.10835300648896354	No Hit
GGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTCGT	52474	0.10810578511810989	No Hit
CGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAA	52423	0.1080007160354971	No Hit
CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC	52332	0.10781323982926645	No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA	51838	0.10679551185258569	No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG	51729	0.10657095244072698	No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA	51325	0.10573864049218643	No Hit
TTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAGAATC	51283	0.10565211301238767	No Hit
CTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACAAA	50685	0.10442012651430044	No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC	50420	0.103874179320332	No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC	50345	0.1037196659635485	No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA	50228	0.10347862512696619	No Hit
GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC	50147	0.10331175070164	No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG	49206	0.10137312311852947	No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT	49158	0.10127423457018804	No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT	48908	0.10075919004757632	No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC	48861	0.1006623616773253	No Hit
GTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCT	48570	0.10006284985300527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.002173487885421465	2.0601780904468862E-6	0.0
2	0.002245594118587106	2.0601780904468862E-6	2.0601780904468862E-6
3	0.00244131103717956	2.0601780904468862E-6	2.0601780904468862E-6
4	0.0026720509833096113	2.0601780904468862E-6	2.0601780904468862E-6
5	0.003020221080595135	2.0601780904468862E-6	2.0601780904468862E-6
6	0.003180914971649992	8.240712361787545E-6	4.1203561808937725E-6
7	0.0033580902874284246	8.240712361787545E-6	4.1203561808937725E-6
8	0.0035950107678298163	8.240712361787545E-6	6.180534271340659E-6
9	0.004511790018078681	8.240712361787545E-6	6.180534271340659E-6
10-11	0.005462562206819919	8.240712361787545E-6	8.240712361787545E-6
12-13	0.006143451065712615	8.240712361787545E-6	1.2361068542681317E-5
14-15	0.007288910084001083	8.240712361787545E-6	1.339115758790476E-5
16-17	0.008377714204802263	8.240712361787545E-6	1.9571691859245417E-5
18-19	0.00929861381123202	8.240712361787545E-6	2.060178090446886E-5
20-21	0.01011547442409421	8.240712361787545E-6	2.060178090446886E-5
22-23	0.010894221742283133	8.240712361787545E-6	2.2661958994915748E-5
24-25	0.011833662951526915	8.240712361787545E-6	2.2661958994915748E-5
26-27	0.013347893848005376	8.240712361787545E-6	2.2661958994915748E-5
28-29	0.015748001323375997	1.030089045223443E-5	2.2661958994915748E-5
30-31	0.023708529464862765	1.1330979497457874E-5	2.2661958994915748E-5
32-33	0.02824504162002681	1.2361068542681317E-5	2.2661958994915748E-5
34-35	0.034053713746041804	1.4421246633128203E-5	2.2661958994915748E-5
36-37	0.04124064501456577	1.4421246633128203E-5	2.2661958994915748E-5
38-39	0.049502989246303	1.4421246633128203E-5	2.2661958994915748E-5
40-41	0.059238360812709764	1.4421246633128203E-5	2.2661958994915748E-5
42-43	0.07122344685388453	1.4421246633128203E-5	2.2661958994915748E-5
44-45	0.08513067905344623	1.4421246633128203E-5	2.2661958994915748E-5
46-47	0.10193967209340238	1.5451335678351646E-5	2.2661958994915748E-5
48-49	0.12181214995385305	1.648142472357509E-5	2.2661958994915748E-5
50-51	0.14607795759218167	2.0601780904468864E-5	2.2661958994915748E-5
52-53	0.1737296679221598	2.2661958994915748E-5	2.2661958994915748E-5
54-55	0.20701287506237448	2.4722137085362635E-5	2.369204804013919E-5
56-57	0.2422841540598704	2.4722137085362635E-5	2.4722137085362635E-5
58-59	0.2797041988057024	2.4722137085362635E-5	2.678231517580952E-5
60-61	0.32326563443915657	2.678231517580952E-5	3.090267135670329E-5
62-63	0.373083830933298	2.8842493266256406E-5	3.193276040192673E-5
64-65	0.4304412491494297	3.090267135670329E-5	3.296284944715018E-5
66-67	0.49743515038362673	3.090267135670329E-5	3.399293849237363E-5
68-69	0.5740016691150853	3.090267135670329E-5	3.81132946732674E-5
70-71	0.6584308575287343	3.090267135670329E-5	4.223365085416116E-5
72-73	0.7611791495226372	3.090267135670329E-5	4.326373989938461E-5
74-75	0.8827760108660385	3.399293849237363E-5	4.326373989938461E-5
76-77	1.008093553840787	3.7083205628043954E-5	4.6354007035054936E-5
78-79	1.1543661982625157	3.7083205628043954E-5	4.738409608027838E-5
80-81	1.3258595428674955	3.81132946732674E-5	4.738409608027838E-5
82-83	1.5119574899556532	3.914338371849084E-5	4.738409608027838E-5
84-85	1.7192845421768208	4.017347276371428E-5	4.738409608027838E-5
86-87	1.9528283906879702	4.120356180893772E-5	4.738409608027838E-5
88-89	2.212669382434629	4.120356180893772E-5	5.3564630351619044E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCAAT	13185	0.0	71.961494	1
TCCAATC	13805	0.0	68.613556	2
AAGAGCA	416850	0.0	67.169655	7
GAGCACA	435530	0.0	64.25603	9
CGGAAGA	437400	0.0	64.18741	4
TCGGAAG	444935	0.0	63.225613	3
AGAGCAC	442890	0.0	63.157112	8
GATCGGA	466795	0.0	60.549793	1
GAAGAGC	474660	0.0	59.042927	6
GGAAGAG	484890	0.0	57.823536	5
TCAAAGG	18745	0.0	53.480846	7
CCAATCA	18195	0.0	52.424324	3
ATCGGAA	542185	0.0	51.973522	2
ATCAAAG	19115	0.0	51.1786	6
CTTGTTA	17845	0.0	50.533794	1
CCGGCAT	2975	0.0	50.4664	1
CAAAGGA	19250	0.0	49.586193	8
CGCGGAT	2825	0.0	47.59597	1
CAATCAA	20940	0.0	46.515522	4
GCCGTCT	302575	0.0	45.97047	48-49
>>END_MODULE