##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	brugia_2_CGATGT_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49143805
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.91712422349063	33.0	33.0	33.0	27.0	33.0
2	30.612993255202767	33.0	33.0	33.0	27.0	33.0
3	30.424769083305616	33.0	33.0	33.0	22.0	33.0
4	33.94952291545191	37.0	37.0	37.0	27.0	37.0
5	33.986083495162816	37.0	37.0	37.0	27.0	37.0
6	34.311630448639455	37.0	37.0	37.0	27.0	37.0
7	33.86610039658101	37.0	37.0	37.0	27.0	37.0
8	34.222035920906	37.0	37.0	37.0	27.0	37.0
9	33.811404082366025	37.0	37.0	37.0	27.0	37.0
10-11	33.90904620633262	37.0	37.0	37.0	27.0	37.0
12-13	33.812520275953396	37.0	37.0	37.0	27.0	37.0
14-15	35.469002339155466	40.0	37.0	40.0	27.0	40.0
16-17	35.44607800515243	40.0	37.0	40.0	24.5	40.0
18-19	35.455970513475705	40.0	37.0	40.0	24.5	40.0
20-21	35.365520170853685	40.0	37.0	40.0	22.0	40.0
22-23	35.76886336945216	40.0	37.0	40.0	27.0	40.0
24-25	35.31216060701853	40.0	37.0	40.0	22.0	40.0
26-27	35.29041993187137	40.0	37.0	40.0	24.5	40.0
28-29	34.76796169934339	38.5	37.0	40.0	15.0	40.0
30-31	34.661629497349665	37.0	37.0	40.0	15.0	40.0
32-33	34.554692437836266	37.0	35.0	40.0	15.0	40.0
34-35	34.69584569204603	38.5	37.0	40.0	15.0	40.0
36-37	34.73602960739405	40.0	37.0	40.0	15.0	40.0
38-39	34.74003033546141	38.5	37.0	40.0	15.0	40.0
40-41	34.751281051599484	37.0	37.0	40.0	18.5	40.0
42-43	34.569627870694994	37.0	35.0	40.0	18.5	40.0
44-45	34.57585079136628	37.0	33.0	40.0	22.0	40.0
46-47	34.12223528479327	37.0	33.0	40.0	15.0	40.0
48-49	33.95270868830771	37.0	33.0	40.0	15.0	40.0
50-51	33.690839109832865	37.0	33.0	40.0	15.0	40.0
52-53	33.549836525682124	37.0	33.0	40.0	15.0	40.0
54-55	33.35846172269323	37.0	33.0	40.0	15.0	40.0
56-57	33.36733775498254	37.0	33.0	40.0	18.5	40.0
58-59	32.974420519534455	37.0	33.0	37.0	15.0	40.0
60-61	33.38068490016188	37.0	33.0	37.0	22.0	40.0
62-63	33.356645278077266	37.0	33.0	37.0	22.0	40.0
64-65	33.170966330344186	37.0	33.0	37.0	22.0	40.0
66-67	33.078660026426526	37.0	33.0	37.0	22.0	38.5
68-69	32.69909768891522	37.0	33.0	37.0	22.0	37.0
70-71	32.231978547448655	37.0	33.0	37.0	15.0	37.0
72-73	32.071561237474384	37.0	33.0	37.0	15.0	37.0
74-75	31.974029544517357	37.0	33.0	37.0	15.0	37.0
76-77	31.872756260936654	37.0	33.0	37.0	15.0	37.0
78-79	31.789146180276436	37.0	33.0	37.0	15.0	37.0
80-81	31.70607724615544	37.0	33.0	37.0	15.0	37.0
82-83	31.626517706555283	37.0	33.0	37.0	15.0	37.0
84-85	31.564961555174655	37.0	33.0	37.0	15.0	37.0
86-87	31.48108212215151	37.0	33.0	37.0	15.0	37.0
88-89	31.391201688595338	37.0	33.0	37.0	15.0	37.0
90-91	31.308801567155818	33.0	33.0	37.0	15.0	37.0
92-93	31.234418600676115	33.0	33.0	37.0	15.0	37.0
94-95	31.201595918346165	33.0	33.0	37.0	15.0	37.0
96-97	31.102768477125448	33.0	33.0	37.0	15.0	37.0
98-99	30.990291563707775	33.0	33.0	37.0	15.0	37.0
100-101	30.546917805814996	33.0	30.0	37.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2201	1	0.17403309565221647
2201	2	0.2472390336712138
2201	3	0.17239228175145627
2201	4	0.2501604308861687
2201	5	0.291812508808583
2201	6	0.3748557109183537
2201	7	0.21889218738353122
2201	8	0.34868698971745715
2201	9	0.3695828586881049
2201	10-11	0.28135500878102704
2201	12-13	0.17800686250577513
2201	14-15	0.417436638146917
2201	16-17	0.5475863176410414
2201	18-19	0.4403639821943699
2201	20-21	0.40899388539268955
2201	22-23	0.42651911053919633
2201	24-25	0.5027494018907674
2201	26-27	0.5720074664899641
2201	28-29	0.6911093203630969
2201	30-31	0.9139912363391716
2201	32-33	0.8416129819448841
2201	34-35	0.7155250061739409
2201	36-37	0.6958957954777674
2201	38-39	0.6413212852860752
2201	40-41	0.6223290800242012
2201	42-43	0.6335065368464399
2201	44-45	0.7073677453047935
2201	46-47	0.6395339412788488
2201	48-49	0.696546909552211
2201	50-51	0.627863107083428
2201	52-53	0.6476741363365903
2201	54-55	0.6321052099403275
2201	56-57	0.6632031061381269
2201	58-59	0.6549409774529167
2201	60-61	0.6216350826961019
2201	62-63	0.6107983354672157
2201	64-65	0.6302396271588506
2201	66-67	0.5912988449115062
2201	68-69	0.6482267167690026
2201	70-71	0.6282766477642596
2201	72-73	0.5625059312423701
2201	74-75	0.5256440409815042
2201	76-77	0.5770280637517899
2201	78-79	0.5621539474222033
2201	80-81	0.5654555033783524
2201	82-83	0.5302305076711704
2201	84-85	0.5915420321245577
2201	86-87	0.635277799191428
2201	88-89	0.5834149283040979
2201	90-91	0.6380367390154937
2201	92-93	0.6327509771497439
2201	94-95	0.6013186213307407
2201	96-97	0.6574532283889987
2201	98-99	0.706752497169095
2201	100-101	0.6667853282083627
1101	1	0.31585911583529835
1101	2	0.3473311574882345
1101	3	0.30953487249801626
1101	4	0.4013806641171982
1101	5	0.33231107662034987
1101	6	0.33101560331068214
1101	7	0.36600903223061465
1101	8	0.3759365212094252
1101	9	0.3330076885512767
1101	10-11	0.37075535587682396
1101	12-13	0.4062651828718984
1101	14-15	0.529412257568147
1101	16-17	0.5192404589430453
1101	18-19	0.5659573471468775
1101	20-21	0.5705283758206221
1101	22-23	0.6707775200915904
1101	24-25	0.6696881728123003
1101	26-27	0.6611202933843288
1101	28-29	0.9068984543560319
1101	30-31	0.8935692247116407
1101	32-33	0.8117848449523706
1101	34-35	0.6628217078549099
1101	36-37	0.6788749419560816
1101	38-39	0.6607953803635169
1101	40-41	0.666098647587333
1101	42-43	0.5821462227458838
1101	44-45	0.6824503133335327
1101	46-47	0.6897655760332597
1101	48-49	0.6984034183852259
1101	50-51	0.7171082321164093
1101	52-53	0.7013362774922953
1101	54-55	0.6951784138497885
1101	56-57	0.6915656479858825
1101	58-59	0.6722693269298645
1101	60-61	0.6677350199080934
1101	62-63	0.7206138013147623
1101	64-65	0.7304699159256671
1101	66-67	0.7121850419310221
1101	68-69	0.6398263062861957
1101	70-71	0.6712157345610237
1101	72-73	0.6525476847300169
1101	74-75	0.68592816635757
1101	76-77	0.6675929897324835
1101	78-79	0.6430931862039841
1101	80-81	0.6436678372355473
1101	82-83	0.6485817451606337
1101	84-85	0.6646579067354139
1101	86-87	0.6077182700187294
1101	88-89	0.5958293074676426
1101	90-91	0.6712893847576673
1101	92-93	0.6644219007181107
1101	94-95	0.6972144557241613
1101	96-97	0.7295510734749975
1101	98-99	0.6758618040467788
1101	100-101	0.717031977400719
2202	1	0.27005991214227976
2202	2	0.289436769151024
2202	3	0.2537196220839597
2202	4	0.293217974053654
2202	5	0.2991484178282917
2202	6	0.4325827852838131
2202	7	0.35841328161325947
2202	8	0.38876283226869646
2202	9	0.33841399606112077
2202	10-11	0.36936544345500977
2202	12-13	0.3811414825229562
2202	14-15	0.5459101622079743
2202	16-17	0.5404948335074309
2202	18-19	0.5703940590688745
2202	20-21	0.5691616686535355
2202	22-23	0.6163028989922381
2202	24-25	0.6074162906593727
2202	26-27	0.6149090182232797
2202	28-29	0.8158331375336445
2202	30-31	0.8935111789542844
2202	32-33	0.8139202100655965
2202	34-35	0.6184005267850452
2202	36-37	0.5752539984047615
2202	38-39	0.590409595687376
2202	40-41	0.5835243844161724
2202	42-43	0.5995678768546711
2202	44-45	0.6639515899151291
2202	46-47	0.6318427297608409
2202	48-49	0.6484572872542387
2202	50-51	0.6491929769591707
2202	52-53	0.6333807871696777
2202	54-55	0.6259194389413523
2202	56-57	0.6847372894166739
2202	58-59	0.6966907449376194
2202	60-61	0.6351140146258913
2202	62-63	0.622749478877445
2202	64-65	0.5813816153019573
2202	66-67	0.6193436403769681
2202	68-69	0.6231925206341558
2202	70-71	0.5621895768960385
2202	72-73	0.6272741318270576
2202	74-75	0.6564804922101644
2202	76-77	0.6504671764697676
2202	78-79	0.5458962224504873
2202	80-81	0.5518949453622284
2202	82-83	0.5423572008675066
2202	84-85	0.46586325008073004
2202	86-87	0.5588228928232297
2202	88-89	0.5414424121699319
2202	90-91	0.5753794420603136
2202	92-93	0.565650075387282
2202	94-95	0.6334676836556596
2202	96-97	0.6025251311329711
2202	98-99	0.6200048289804521
2202	100-101	0.69296473349943
1102	1	0.16309609273947245
1102	2	0.2107743218216953
1102	3	0.1392698578765028
1102	4	0.15939680362281905
1102	5	0.15524281030091203
1102	6	0.3184400410219368
1102	7	0.2156347422409226
1102	8	0.20964435753228372
1102	9	0.19068657204054063
1102	10-11	0.1984664976930688
1102	12-13	0.21929573790242074
1102	14-15	0.29602002921155446
1102	16-17	0.3005016219084169
1102	18-19	0.31877726450154853
1102	20-21	0.34949131211494944
1102	22-23	0.44502982008092573
1102	24-25	0.40564468201786497
1102	26-27	0.4808014068879629
1102	28-29	0.6998894519029477
1102	30-31	0.703293559523658
1102	32-33	0.6098215348561453
1102	34-35	0.4589875653845539
1102	36-37	0.44348878205980213
1102	38-39	0.4257276707265092
1102	40-41	0.4214246371700838
1102	42-43	0.345940215219791
1102	44-45	0.49420821071439036
1102	46-47	0.3945506741325957
1102	48-49	0.4089228451873481
1102	50-51	0.38096864716316503
1102	52-53	0.37029729139340617
1102	54-55	0.3180368376593634
1102	56-57	0.3642913911371011
1102	58-59	0.36070303721189845
1102	60-61	0.4547773439403002
1102	62-63	0.5188023632871648
1102	64-65	0.3517417238579412
1102	66-67	0.4286143993818996
1102	68-69	0.3114742349938098
1102	70-71	0.3334082089653734
1102	72-73	0.34008384014305904
1102	74-75	0.31636343629348573
1102	76-77	0.3605785347852475
1102	78-79	0.28932823271507857
1102	80-81	0.3482817114659085
1102	82-83	0.3877632176802379
1102	84-85	0.3848854794516363
1102	86-87	0.32612729462151435
1102	88-89	0.38280560653050344
1102	90-91	0.36249120145084035
1102	92-93	0.3625824514482048
1102	94-95	0.4116902706548409
1102	96-97	0.4221054201949386
1102	98-99	0.3780698003346217
1102	100-101	0.4519692162455229
2203	1	0.12937007579541415
2203	2	0.17348282333193765
2203	3	0.0599665379996992
2203	4	0.1576253768120992
2203	5	0.1559830513954239
2203	6	0.2201200723781227
2203	7	0.1514569761319322
2203	8	0.11181423410864255
2203	9	0.1389790617368547
2203	10-11	0.13485904574005758
2203	12-13	0.09809124813684633
2203	14-15	0.14870053040001352
2203	16-17	0.1838180936093181
2203	18-19	0.2679963920971531
2203	20-21	0.17703967416709077
2203	22-23	0.3136625669882065
2203	24-25	0.2388380193364057
2203	26-27	0.3209846943614707
2203	28-29	0.4854821925001005
2203	30-31	0.4898683389283178
2203	32-33	0.41215381577202237
2203	34-35	0.2617385404543384
2203	36-37	0.21685591268847304
2203	38-39	0.16343740574567533
2203	40-41	0.2144610369233675
2203	42-43	0.170809128447722
2203	44-45	0.24517333202627611
2203	46-47	0.19826576562200415
2203	48-49	0.12073486857340043
2203	50-51	0.1888026484479397
2203	52-53	0.19824260789238224
2203	54-55	0.16256547446455727
2203	56-57	0.21886408967952775
2203	58-59	0.1426469552427605
2203	60-61	0.23103873796422647
2203	62-63	0.2210386854129709
2203	64-65	0.22325843134976964
2203	66-67	0.1921849232119115
2203	68-69	0.2173386231399732
2203	70-71	0.1760395349575461
2203	72-73	0.18458446612667956
2203	74-75	0.14701920618585973
2203	76-77	0.15665782516621363
2203	78-79	0.14653839387387535
2203	80-81	0.1859822731048162
2203	82-83	0.19351419751014376
2203	84-85	0.1911029178471182
2203	86-87	0.20574064201910858
2203	88-89	0.1920752228119298
2203	90-91	0.21426480013935745
2203	92-93	0.22938139604856644
2203	94-95	0.18089392718156105
2203	96-97	0.2965436812173543
2203	98-99	0.261915492699643
2203	100-101	0.23566935381539977
1103	1	0.14923212019159493
1103	2	0.13106891931031583
1103	3	0.11910210096420215
1103	4	0.13643943018789884
1103	5	0.18848763079792263
1103	6	0.1653741232064263
1103	7	0.18342155469839838
1103	8	0.21480956914187743
1103	9	0.16444580375104323
1103	10-11	0.16790039587318972
1103	12-13	0.1297054128232773
1103	14-15	0.1748467363056605
1103	16-17	0.2068192630263468
1103	18-19	0.23353464083129438
1103	20-21	0.2328656600490433
1103	22-23	0.28733575407202494
1103	24-25	0.33831697556650653
1103	26-27	0.3970725165403408
1103	28-29	0.6346692656054245
1103	30-31	0.6556358372687612
1103	32-33	0.5511605873865264
1103	34-35	0.3347339419941804
1103	36-37	0.35972726317621095
1103	38-39	0.3142736355705935
1103	40-41	0.3380457373067003
1103	42-43	0.36810235332837493
1103	44-45	0.35574626321903224
1103	46-47	0.30822982665389276
1103	48-49	0.3043819210677867
1103	50-51	0.37638043801442933
1103	52-53	0.3756881207804881
1103	54-55	0.33162718762526566
1103	56-57	0.3724061317332783
1103	58-59	0.3917663958143507
1103	60-61	0.4147295509743074
1103	62-63	0.41691275518248005
1103	64-65	0.3657675203445052
1103	66-67	0.38388117601564176
1103	68-69	0.3749044206175256
1103	70-71	0.2922076292400959
1103	72-73	0.33871180744436913
1103	74-75	0.29764253764754756
1103	76-77	0.2937467401999285
1103	78-79	0.2558643936114464
1103	80-81	0.3073202564034929
1103	82-83	0.2416579308376292
1103	84-85	0.29971065531795205
1103	86-87	0.3336190203502518
1103	88-89	0.3205958506296973
1103	90-91	0.28533031494783145
1103	92-93	0.23714049645966995
1103	94-95	0.2879183510706582
1103	96-97	0.2974524671461758
1103	98-99	0.28349103200708825
1103	100-101	0.30067578105548876
2204	1	0.17732359919039808
2204	2	0.16670575690830702
2204	3	0.14529464329928388
2204	4	0.18297517357356696
2204	5	0.20410254652188797
2204	6	0.20792959635646469
2204	7	0.18896860615669198
2204	8	0.16552003802455317
2204	9	0.1031734077003108
2204	10-11	0.17561303968244601
2204	12-13	0.16086243639440312
2204	14-15	0.12127447084593967
2204	16-17	0.17089359082132916
2204	18-19	0.10782033043156503
2204	20-21	0.08414445750278077
2204	22-23	0.09635038943007146
2204	24-25	-0.11382534178632397
2204	26-27	-0.13881806244630468
2204	28-29	0.04592424557661445
2204	30-31	0.08461906419503151
2204	32-33	0.0025562091366637674
2204	34-35	-0.22182073928026824
2204	36-37	-0.3607842788920337
2204	38-39	-0.2517116876028851
2204	40-41	-0.05866624960853528
2204	42-43	-0.015769958465845946
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1115	10-11	-0.3420155256458344
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1115	14-15	-0.5114200609391801
1115	16-17	-0.5473320590042405
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1115	22-23	-0.5632337242560652
1115	24-25	-0.4591193201570505
1115	26-27	-0.4625048386787398
1115	28-29	-0.2877914384841631
1115	30-31	-0.21969275119472798
1115	32-33	-0.35726767307323115
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1213	72-73	-1.4041652093602117
1213	74-75	-1.4875472811796868
1213	76-77	-1.5260547874818542
1213	78-79	-1.4833011509205676
1213	80-81	-1.5774032159849867
1213	82-83	-1.4041716122604484
1213	84-85	-1.3201160739515032
1213	86-87	-1.3802477581019872
1213	88-89	-1.6470817077954898
1213	90-91	-1.695056685544504
1213	92-93	-1.840973963792404
1213	94-95	-1.7914556655235963
1213	96-97	-1.777938957081819
1213	98-99	-1.957526646703581
1213	100-101	-1.930185015650693
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5124.0
3	19715.0
4	13856.0
5	3085.0
6	2760.0
7	10127.0
8	54309.0
9	235212.0
10	941869.0
11	1503000.0
12	651910.0
13	252435.0
14	170009.0
15	147729.0
16	146296.0
17	153941.0
18	171466.0
19	192389.0
20	217957.0
21	245661.0
22	276093.0
23	307467.0
24	345899.0
25	391230.0
26	450412.0
27	527110.0
28	625009.0
29	748171.0
30	908022.0
31	1134964.0
32	1478509.0
33	1980600.0
34	2642939.0
35	3633889.0
36	6238494.0
37	1.5615823E7
38	6697316.0
39	3008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.56698036097071	11.77439271563942	12.017721644108391	36.640905279281476
2	21.281892432032024	23.270579806569735	38.05870846093795	17.38881930046029
3	23.035788667905837	24.55603036712956	26.234266239675563	26.173914725289045
4	24.157915180221135	34.83494169830493	19.014769198225437	21.9923739232485
5	26.769281178638977	32.54483109034979	20.737055221814167	19.948832509197075
6	21.677128089208043	34.01624406533425	25.667576813107722	18.639051032349986
7	18.433972593482267	16.309608707896302	41.898514198756644	23.35790449986479
8	18.057430523558697	23.15506795903283	28.154687372732866	30.632814144675603
9	26.759463397391837	22.710492280728044	27.379159350271536	23.150884971608587
10-11	25.403691049599068	33.88027092978268	19.710055346662934	21.005982673955316
12-13	25.762121857252424	22.38390695712489	27.99460215431113	23.85936903131156
14-15	24.668873937784173	25.32687412798808	27.284305956455164	22.719945977772586
16-17	26.817077032035964	25.343978301477755	26.283053286237283	21.555891380248998
18-19	26.16792436656349	25.100566693223335	25.781410025436507	22.950098914776664
20-21	25.59437871389238	26.453015772030376	25.640203795490436	22.31240171858681
22-23	28.150390852847433	25.45003501163704	24.33506024517402	22.064513890341505
24-25	24.73314391337903	28.74142332688271	24.61323060221635	21.912202157521904
26-27	23.972645822783928	29.29174216331114	25.41365382900944	21.321958184895493
28-29	24.670152637034512	27.985765182349454	24.480590170364145	22.86349201025189
30-31	26.876944111925745	25.855868441168546	24.916113958849905	22.351073488055807
32-33	23.695839279298507	27.35458332857942	25.883086681870076	23.066490710252
34-35	23.16026748621856	29.31710928108765	25.806998296434514	21.715624936259275
36-37	22.124011791044886	28.155783243070964	26.894443663016098	22.825761302868052
38-39	22.030827414066138	25.338793175567666	28.616710194401225	24.013669215964974
40-41	26.6122080229589	26.2211462276214	25.770793415234344	21.395852334185356
42-43	26.725244808832038	25.518597546437395	26.153667758096244	21.60248988663432
44-45	27.3564806599262	26.404561743688305	25.89477356840871	20.344184027976798
46-47	26.638068930189068	24.753104217040935	25.614168387483016	22.99465846528698
48-49	23.341530823621344	25.248920765093434	25.13125365974549	26.27829475153974
50-51	25.486051676702587	25.34596212489288	25.701202919658233	23.466783278746302
52-53	23.596379476774402	27.109887827837348	27.474663198524112	21.81906949686414
54-55	22.91602965240624	26.952172379361826	25.943062336495288	24.18873563173664
56-57	21.53082348423812	25.615880315860988	30.49109892018165	22.362197279719247
58-59	22.57727561089327	28.823602471957376	27.44148104914204	21.157640868007316
60-61	22.324394622839097	31.464515524699866	25.289600440235777	20.92148941222526
62-63	21.1697439633892	31.856993084851176	25.441190585575114	21.532072366184508
64-65	21.340651393767278	31.96665791677637	25.010664215271376	21.68202647418497
66-67	22.96112053831855	31.4940699346111	24.070738146726622	21.474071380343723
68-69	21.64509640859114	30.64666712134346	26.121173483314745	21.587062986750652
70-71	22.17365655075211	29.04674835913128	26.037549887687994	22.742045202428613
72-73	23.027738749505016	29.04898631627646	25.208722415151875	22.714552519066654
74-75	23.269626852243537	28.833024512994776	26.36514895512801	21.53219967963367
76-77	23.72530747276957	27.484561024006698	26.030477113397605	22.759654389826125
78-79	23.901725034499908	27.76953410125063	25.141944732108424	23.186796132141037
80-81	24.95572435436857	27.185541704067912	25.629117793887424	22.229616147676097
82-83	24.089016429348693	27.37232435309617	25.837204624955646	22.701454592599493
84-85	23.751115167565942	28.320684313738585	24.84367391262202	23.084526606073453
86-87	22.802070529414166	29.0183285663279	25.744633646583605	22.434967257674327
88-89	23.25354273257274	27.841903169620725	27.02075485447858	21.883799243327957
90-91	24.380640445046716	26.89073253633771	26.647978911931613	22.080648106683963
92-93	23.33947188568182	26.60229441851295	27.7578372520039	22.300396443801336
94-95	23.687992597047753	27.074867697286724	25.62939533100418	23.607744374661337
96-97	22.72387040832033	29.366680403904333	25.38239723276688	22.52705195500846
98-99	24.04726812892921	28.58464285864931	25.46948075306347	21.898608259358017
100-101	23.568814660999955	27.224692380342404	25.898221868258364	23.308271090399284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4264.0
1	4440.5
2	6281.0
3	9446.0
4	10433.5
5	8704.0
6	6221.5
7	4399.5
8	3671.5
9	3681.5
10	4027.0
11	4517.5
12	5013.5
13	5664.5
14	6588.5
15	8110.0
16	11837.0
17	16478.5
18	20170.5
19	20799.0
20	21151.0
21	24101.0
22	29935.5
23	36680.0
24	46188.0
25	73972.5
26	119533.0
27	161959.5
28	168243.0
29	150786.5
30	155021.5
31	179358.5
32	209598.0
33	243767.0
34	268439.5
35	331862.5
36	550190.5
37	750482.0
38	838571.5
39	916024.0
40	1070688.0
41	1350895.5
42	1691820.5
43	1858123.0
44	2023732.0
45	2497934.5
46	3192998.0
47	3659775.0
48	4042270.0
49	4334485.5
50	3841341.5
51	3213056.5
52	2764826.0
53	2332978.5
54	1900578.5
55	1243839.0
56	750060.5
57	475039.5
58	256872.0
59	169556.0
60	138868.5
61	150803.5
62	211489.5
63	208682.5
64	139196.0
65	78604.0
66	42010.0
67	28140.0
68	10724.0
69	5811.0
70	3745.0
71	2625.5
72	1980.5
73	1550.0
74	1280.0
75	1089.0
76	940.0
77	807.5
78	702.5
79	664.0
80	608.0
81	537.5
82	497.5
83	446.0
84	394.5
85	341.0
86	274.0
87	224.5
88	170.0
89	147.0
90	127.5
91	105.5
92	98.5
93	76.5
94	64.0
95	61.0
96	57.5
97	50.0
98	42.5
99	53.5
100	198.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11631781462587197
2	0.07005359068147043
3	0.05067780160693703
4	0.03480194502643822
5	0.028044226530688866
6	0.02770644234812506
7	0.03345284314065628
8	0.04522645326303081
9	0.06178805243102361
10-11	0.07631073743679391
12-13	0.09449817733893418
14-15	0.11065789472345497
16-17	0.13015882673309484
18-19	0.146542377009676
20-21	0.17179581434526692
22-23	0.19208728343277448
24-25	0.2196909661349991
26-27	0.23316061912584912
28-29	0.26375450578155274
30-31	0.29482861573294944
32-33	0.303387171587548
34-35	0.28809226310416947
36-37	0.24485181804705597
38-39	0.2139588092537808
40-41	0.1899995330031934
42-43	0.16810969358192757
44-45	0.15319835328176157
46-47	0.1443182920003854
48-49	0.13591234948128253
50-51	0.1319423679139212
52-53	0.12774244892107967
54-55	0.12009550339050874
56-57	0.11680210761051163
58-59	0.11918796275542766
60-61	0.11676141072104612
62-63	0.11482729104919735
64-65	0.11307732480218004
66-67	0.11091225028261446
68-69	0.10985921826769417
70-71	0.10715389254047382
72-73	0.09981726079207745
74-75	0.09349805128031904
76-77	0.08748508586178869
78-79	0.08517757222909378
80-81	0.07921853018910523
82-83	0.07731086349540904
84-85	0.07951561748220351
86-87	0.0834306582487864
88-89	0.09181828716762978
90-91	0.09880085597767613
92-93	0.10833613717944714
94-95	0.11798536967172159
96-97	0.13086390034308495
98-99	0.14084379506226674
100-101	0.14958039980827698
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4.9143805E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.217779016902114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.3558211783862	13.293615825326988
2	6.937739873943251	2.111539845568374
3	2.0590161042148467	0.9400095619855269
4	0.9061165191365557	0.5515632380713864
5	0.6591025227992688	0.501503827072098
6	0.35020596351855254	0.3197614177935969
7	0.3178863996643111	0.3386267487799079
8	0.14940180727412375	0.1818850950258731
9	0.1328235051902247	0.18191508752116717
>10	0.8293981278054409	2.357877231521798
>50	0.08359984961136749	0.8810489114202187
>100	0.11569725324859549	3.9376417833828703
>500	0.028508166353560974	3.0514271045253576
>1k	0.052012470559888106	18.290292645706643
>5k	0.011356524655516963	12.108189743977672
>10k+	0.011313733638023073	40.95310193232071
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	141318	0.2875601512744078	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	138296	0.28141085127616794	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	132446	0.2695070111075038	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	128423	0.2613208317915147	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	128025	0.2605109636911509	No Hit
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	127658	0.25976417576945865	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	122328	0.24891845472689794	No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG	119885	0.24394732967868482	No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA	118276	0.24067326492118388	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	111279	0.22643545814167218	No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT	109998	0.2238288223714057	No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC	104464	0.2125679930562967	No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA	103487	0.21057995000590612	No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC	102038	0.20763146036412933	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	99157	0.20176907343662137	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	98739	0.20091850844679202	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	92982	0.1892039088141425	No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA	92333	0.18788329475098642	No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC	88790	0.1806738407821698	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	86400	0.17581056249104032	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	83047	0.16898772897214612	No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT	80931	0.16468199806669429	No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG	78600	0.15993877559948808	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	77379	0.15745423049761817	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	76693	0.15605832718895088	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG	70026	0.14249201908562026	No Hit
CTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAGAAT	67201	0.13674358344861576	No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA	66956	0.13624504655266315	No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG	66442	0.13519913649339932	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	66418	0.1351503002260407	No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA	66205	0.13471687835323293	No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC	65250	0.13277360188125442	No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT	64115	0.13046405340408623	No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT	63855	0.1299349938410345	No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC	63336	0.1288789095594043	No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG	62149	0.12646354916962577	No Hit
GCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTCTTCATTAATCA	59482	0.12103661895939885	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	58541	0.1191218303100462	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	58515	0.11906892435374103	No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC	57517	0.11703814956941165	No Hit
GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA	57413	0.11682652574419095	No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC	57200	0.11639310387138319	No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT	57198	0.11638903418243662	No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT	56717	0.1154102739907909	No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG	55907	0.1137620499674374	No Hit
CCAAGATCTGCACCAATGGAAGCTTCAACCGGGCCTACGCCCAAAGTCTT	54994	0.11190423696333647	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	54890	0.11169261313811578	No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG	54280	0.11045135800941747	No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT	54218	0.11032519765207435	No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA	54194	0.11027636138471575	No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA	53990	0.10986125311216745	No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA	53983	0.10984700920085451	No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT	53922	0.10972288368798469	No Hit
GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC	52671	0.1071772932519165	No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA	52330	0.10648341128652941	No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG	51536	0.10486774477474832	No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC	51427	0.10464594672716122	No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG	51022	0.10382183471548448	No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT	50619	0.10300179239275428	No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT	49987	0.10171577068564389	No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA	49400	0.10052131697983094	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.002458092123717323	0.0	2.0348444732759297E-6
2	0.0025761131031673268	0.0	2.0348444732759297E-6
3	0.0027612839502354366	0.0	2.0348444732759297E-6
4	0.0029993607536087205	0.0	4.0696889465518594E-6
5	0.0033452843140656286	0.0	4.0696889465518594E-6
6	0.003501967338507875	4.0696889465518594E-6	4.0696889465518594E-6
7	0.0036586503629501216	4.0696889465518594E-6	4.0696889465518594E-6
8	0.00387434387711737	4.0696889465518594E-6	4.0696889465518594E-6
9	0.004883626735862232	4.0696889465518594E-6	6.104533419827789E-6
10-11	0.005774888615157088	4.0696889465518594E-6	6.104533419827789E-6
12-13	0.006462666047124353	4.0696889465518594E-6	6.104533419827789E-6
14-15	0.007535029084540767	4.0696889465518594E-6	1.1191644603017614E-5
16-17	0.00859416563288089	6.104533419827789E-6	1.933102249612133E-5
18-19	0.009353162621412812	6.104533419827789E-6	2.0348444732759295E-5
20-21	0.010005330275097746	8.139377893103719E-6	2.1365866969397263E-5
22-23	0.010746013663370184	8.139377893103719E-6	2.4418133679311157E-5
24-25	0.011626083898062025	8.139377893103719E-6	2.4418133679311157E-5
26-27	0.01312779911933966	9.156800129741683E-6	2.4418133679311157E-5
28-29	0.015569612487270776	1.2209066839655577E-5	2.4418133679311157E-5
30-31	0.02326132459625379	1.7296178022845402E-5	2.4418133679311157E-5
32-33	0.02785905568362075	2.2383289206035224E-5	2.4418133679311157E-5
34-35	0.03360850874286189	2.4418133679311157E-5	2.4418133679311157E-5
36-37	0.04059616466409144	2.4418133679311157E-5	2.4418133679311157E-5
38-39	0.04878641366902706	2.4418133679311157E-5	2.4418133679311157E-5
40-41	0.05879072652188816	2.6452978152587086E-5	2.4418133679311157E-5
42-43	0.0708888943377502	2.6452978152587086E-5	2.4418133679311157E-5
44-45	0.08503716796043774	2.6452978152587086E-5	2.543555591594912E-5
46-47	0.10209425175767323	2.6452978152587086E-5	2.6452978152587086E-5
48-49	0.1221740970199601	2.8487822625863014E-5	2.6452978152587086E-5
50-51	0.14635822358484452	2.8487822625863014E-5	3.866204499224266E-5
52-53	0.17418166949018293	3.052266709913895E-5	4.171431170215656E-5
54-55	0.20812592757113535	3.052266709913895E-5	4.273173393879453E-5
56-57	0.24392494638947881	3.052266709913895E-5	4.273173393879453E-5
58-59	0.2816529977684878	3.357493380905284E-5	4.4766578412070456E-5
60-61	0.325979032352094	3.76446227556047E-5	4.6801422885346384E-5
62-63	0.3768053776055802	3.866204499224266E-5	4.781884512198435E-5
64-65	0.436294055781802	4.069688946551859E-5	4.883626735862231E-5
66-67	0.5046393945279573	4.171431170215656E-5	4.9853689595260274E-5
68-69	0.5832902031090186	4.4766578412070456E-5	5.9010489725001964E-5
70-71	0.6711273170646839	4.5784000648708417E-5	6.308017867155382E-5
72-73	0.7767510065612543	4.6801422885346384E-5	6.308017867155382E-5
74-75	0.9017799903772206	4.781884512198435E-5	6.308017867155382E-5
76-77	1.0308939244732882	4.883626735862231E-5	6.308017867155382E-5
78-79	1.1809738785997543	4.9853689595260274E-5	6.308017867155382E-5
80-81	1.3571842880297933	5.290595630517417E-5	6.409760090819178E-5
82-83	1.548118832068457	5.392337854181213E-5	6.61324453814677E-5
84-85	1.760691912235937	5.595822301508807E-5	6.714986761810567E-5
86-87	2.0008656228389317	5.901048972500196E-5	6.918471209138161E-5
88-89	2.266161523308991	6.10453341982779E-5	7.223697880129551E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGCGGC	17575	0.0	80.36785	9
GAGAGCG	21550	0.0	77.0986	7
GGAGAGC	23325	0.0	75.64424	6
AACGGGA	33275	0.0	75.3341	2
GATTGGG	14355	0.0	73.80703	1
ACGGGAG	21110	0.0	73.7416	3
AAGGGGC	35250	0.0	73.540115	9
GTCCAAT	14465	0.0	72.195175	1
GGGAGAG	75400	0.0	71.87496	5
AGAGCGG	23310	0.0	71.78851	8
TCCAATC	14725	0.0	71.512505	2
AAGCGGC	12905	0.0	69.75432	9
GAGGGGC	55375	0.0	68.57383	9
GATCCGG	13235	0.0	67.135345	1
ATCCGGA	13005	0.0	66.80615	2
TTGGGAG	11035	0.0	64.91967	3
ATTGGGA	16090	0.0	64.44249	2
CGGGAAA	50820	0.0	56.476364	4
CGGGAGA	78315	0.0	56.324524	4
TGGGAGA	16010	0.0	55.53665	4
>>END_MODULE