##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	brugia_2_CGATGT_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49143805
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.705393772419534	33.0	33.0	33.0	33.0	33.0
2	32.66068473940917	33.0	33.0	33.0	33.0	33.0
3	32.655434067427215	33.0	33.0	33.0	33.0	33.0
4	36.539390570184786	37.0	37.0	37.0	37.0	37.0
5	36.431000367187686	37.0	37.0	37.0	37.0	37.0
6	36.37671212068337	37.0	37.0	37.0	37.0	37.0
7	36.37269338424243	37.0	37.0	37.0	37.0	37.0
8	36.367529579771045	37.0	37.0	37.0	37.0	37.0
9	36.36886669642288	37.0	37.0	37.0	37.0	37.0
10-11	36.34852571956934	37.0	37.0	37.0	37.0	37.0
12-13	36.335782780759445	37.0	37.0	37.0	37.0	37.0
14-15	38.29635540023814	40.0	37.0	40.0	37.0	40.0
16-17	38.30701039530822	40.0	37.0	40.0	37.0	40.0
18-19	38.29703629989579	40.0	37.0	40.0	37.0	40.0
20-21	38.305177987337366	40.0	37.0	40.0	37.0	40.0
22-23	38.252559351071824	40.0	37.0	40.0	37.0	40.0
24-25	38.223128367858365	40.0	37.0	40.0	37.0	40.0
26-27	38.16089945619799	40.0	37.0	40.0	37.0	40.0
28-29	38.07867147242669	40.0	37.0	40.0	33.0	40.0
30-31	37.99508595233927	40.0	37.0	40.0	33.0	40.0
32-33	37.96884101668562	40.0	37.0	40.0	33.0	40.0
34-35	37.924317134173876	40.0	37.0	40.0	33.0	40.0
36-37	37.75155504340781	40.0	37.0	40.0	33.0	40.0
38-39	37.590104927772686	40.0	37.0	40.0	33.0	40.0
40-41	37.507551755506114	40.0	37.0	40.0	33.0	40.0
42-43	37.36923249023961	40.0	37.0	40.0	33.0	40.0
44-45	37.405019127029334	40.0	37.0	40.0	33.0	40.0
46-47	37.22432116316594	40.0	37.0	40.0	33.0	40.0
48-49	37.15933439626826	40.0	37.0	40.0	33.0	40.0
50-51	36.91539714924394	37.0	37.0	40.0	33.0	40.0
52-53	36.64513321668926	37.0	37.0	40.0	33.0	40.0
54-55	36.49081861894902	37.0	37.0	40.0	33.0	40.0
56-57	36.27325936605845	37.0	37.0	40.0	33.0	40.0
58-59	35.92552402077943	37.0	37.0	40.0	33.0	40.0
60-61	35.73893889575706	37.0	37.0	37.0	33.0	40.0
62-63	35.66218121490593	37.0	37.0	37.0	33.0	40.0
64-65	35.258208120026524	37.0	33.0	37.0	33.0	40.0
66-67	35.16297620829319	37.0	33.0	37.0	33.0	38.5
68-69	34.88586630603796	37.0	33.0	37.0	30.0	37.0
70-71	34.54337297447766	37.0	33.0	37.0	27.0	37.0
72-73	34.09727802924499	37.0	33.0	37.0	27.0	37.0
74-75	33.29606816159229	37.0	33.0	37.0	27.0	37.0
76-77	33.03918036057648	37.0	33.0	37.0	27.0	37.0
78-79	33.00314245101697	37.0	33.0	37.0	24.5	37.0
80-81	33.02044050109673	37.0	33.0	37.0	24.5	37.0
82-83	33.12402771824445	37.0	33.0	37.0	27.0	37.0
84-85	33.07919155832561	37.0	33.0	37.0	27.0	37.0
86-87	33.04563697296129	37.0	33.0	37.0	27.0	37.0
88-89	33.035015695264136	37.0	33.0	37.0	27.0	37.0
90-91	32.99031240051518	37.0	33.0	37.0	27.0	37.0
92-93	32.96375190118063	37.0	33.0	37.0	22.0	37.0
94-95	32.93836255658266	37.0	33.0	37.0	22.0	37.0
96-97	32.87352973584361	37.0	33.0	37.0	22.0	37.0
98-99	32.808599832674744	37.0	33.0	37.0	22.0	37.0
100-101	32.43957923079013	35.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.07856883963274441
2201	2	0.11368495947591839
2201	3	0.10871606669999778
2201	4	0.13472919585540666
2201	5	0.12445519129691718
2201	6	0.2084639127389778
2201	7	0.16899042922278085
2201	8	0.14647243273306287
2201	9	0.19294831615722785
2201	10-11	0.2013106313777513
2201	12-13	0.2092565540431366
2201	14-15	0.39380093570432706
2201	16-17	0.4440864626774754
2201	18-19	0.45281438611668534
2201	20-21	0.4476858597539959
2201	22-23	0.3503308145992605
2201	24-25	0.11302316404525214
2201	26-27	0.17349823077019977
2201	28-29	0.2098774663488996
2201	30-31	0.12832956694178677
2201	32-33	0.2968861950283781
2201	34-35	0.16806491374887855
2201	36-37	0.3904333645894411
2201	38-39	0.48300340122388974
2201	40-41	0.49790697753226
2201	42-43	0.4459680665078807
2201	44-45	0.49389475804604643
2201	46-47	0.5207103064856327
2201	48-49	0.51012953973121
2201	50-51	0.4977310291116055
2201	52-53	0.5642162081024225
2201	54-55	0.5003465896963064
2201	56-57	0.4464511947412646
2201	58-59	0.513327567048897
2201	60-61	0.5105342769277925
2201	62-63	0.45718191052672097
2201	64-65	0.47931635520943416
2201	66-67	0.4617699089556666
2201	68-69	0.3774407462680145
2201	70-71	0.44018556222986405
2201	72-73	0.4894970920699677
2201	74-75	0.4752137224798503
2201	76-77	0.36681492255896586
2201	78-79	0.3748689344556695
2201	80-81	0.24587951117378282
2201	82-83	0.2194659907806198
2201	84-85	0.33774519968213923
2201	86-87	0.3910002053627153
2201	88-89	0.3895307376547663
2201	90-91	0.4085090596406147
2201	92-93	0.39219870200485474
2201	94-95	0.42206017056155787
2201	96-97	0.4759139241864787
2201	98-99	0.48564561348177193
2201	100-101	0.5186040888985701
1101	1	0.08553475799894272
1101	2	0.08833594052696725
1101	3	0.10605221228369999
1101	4	0.12592717229681227
1101	5	0.15215920532839533
1101	6	0.16093126743921005
1101	7	0.17484483868864942
1101	8	0.19250269115983087
1101	9	0.19049207670940405
1101	10-11	0.20273476048701156
1101	12-13	0.09716931569422371
1101	14-15	0.09559441141998803
1101	16-17	0.0582345029277036
1101	18-19	0.2331787715939555
1101	20-21	0.12863636622140007
1101	22-23	0.2022809269953001
1101	24-25	0.3470635944313045
1101	26-27	0.3505887995163519
1101	28-29	0.36125548359450477
1101	30-31	0.41702396116770046
1101	32-33	0.3889771321119895
1101	34-35	0.3719049107315868
1101	36-37	0.44078962715062175
1101	38-39	0.45551685697972744
1101	40-41	0.39501928591560187
1101	42-43	0.4104649959170672
1101	44-45	0.3154215068510169
1101	46-47	0.4366395355876449
1101	48-49	0.4481154994371934
1101	50-51	0.4275036196050763
1101	52-53	0.29861990830869445
1101	54-55	0.3797258197500355
1101	56-57	0.41402591573935155
1101	58-59	0.3507725935750088
1101	60-61	0.40436801257042276
1101	62-63	0.3861700638376675
1101	64-65	0.4440174351747004
1101	66-67	0.40628052707737794
1101	68-69	0.34372247460498073
1101	70-71	0.3903327262248979
1101	72-73	0.29262021748286315
1101	74-75	0.3717176842703793
1101	76-77	0.3995486302066382
1101	78-79	0.351818331956288
1101	80-81	0.4064987900291044
1101	82-83	0.4088432792600756
1101	84-85	0.4197956729611221
1101	86-87	0.38173386498809236
1101	88-89	0.3518801096558377
1101	90-91	0.3421217397905778
1101	92-93	0.3390135883307437
1101	94-95	0.41637400067468633
1101	96-97	0.3164053158488045
1101	98-99	0.35836506720016814
1101	100-101	0.41051546070891476
2202	1	0.06403027260036964
2202	2	0.06829731855439292
2202	3	0.08502564893334608
2202	4	0.10463810053900602
2202	5	0.1360827954003696
2202	6	0.1661975835280316
2202	7	0.148069261996973
2202	8	0.113013752453476
2202	9	0.135630074460515
2202	10-11	0.16081462826802806
2202	12-13	0.170653724504497
2202	14-15	0.2284734354622131
2202	16-17	0.22445325003614158
2202	18-19	0.2027726686978113
2202	20-21	0.26989450482861344
2202	22-23	0.2133779448844706
2202	24-25	0.24845641103186722
2202	26-27	0.2889248940642304
2202	28-29	0.2757384209447764
2202	30-31	0.26419935821262186
2202	32-33	0.18126725353719308
2202	34-35	0.21379636133799806
2202	36-37	0.36397074241686767
2202	38-39	0.3759529001059647
2202	40-41	0.2717720437566413
2202	42-43	0.2413776812872399
2202	44-45	0.34934841439841335
2202	46-47	0.30845395439076384
2202	48-49	0.2830435650096703
2202	50-51	0.2538993620134704
2202	52-53	0.341100604218056
2202	54-55	0.30773163228651157
2202	56-57	0.3410351023159208
2202	58-59	0.3942091178787166
2202	60-61	0.3731910463586985
2202	62-63	0.3494406881497696
2202	64-65	0.3466340714811267
2202	66-67	0.38216540264149046
2202	68-69	0.2386388524978429
2202	70-71	0.3210965423456571
2202	72-73	0.28938617145674783
2202	74-75	0.34734236162452703
2202	76-77	0.3586751048024155
2202	78-79	0.29757670552955773
2202	80-81	0.28939355685300683
2202	82-83	0.306810989470236
2202	84-85	0.26432660623644466
2202	86-87	0.2691427946193983
2202	88-89	0.2943417451754158
2202	90-91	0.2643204593914703
2202	92-93	0.24272471176538346
2202	94-95	0.294578793709114
2202	96-97	0.3508938977329308
2202	98-99	0.28417382244292355
2202	100-101	0.330796939327513
1102	1	0.05947439670940469
1102	2	0.05118586321895435
1102	3	0.06783116634561281
1102	4	0.07772434038827214
1102	5	0.10831123953867206
1102	6	0.13387165979350613
1102	7	0.12227153101139976
1102	8	0.12421171484380977
1102	9	0.08841131114655099
1102	10-11	0.131521179186457
1102	12-13	0.13772633801417555
1102	14-15	0.25755404536555915
1102	16-17	0.2464981796758039
1102	18-19	0.26516569237184484
1102	20-21	0.23166054037326944
1102	22-23	0.261619413574806
1102	24-25	0.23092069518241942
1102	26-27	0.2589311158317429
1102	28-29	0.25796757106172663
1102	30-31	0.2726285332793026
1102	32-33	0.324822756124739
1102	34-35	0.23777171328428892
1102	36-37	0.3228625676242416
1102	38-39	0.34240190416497995
1102	40-41	0.31840177390870394
1102	42-43	0.2824029689403744
1102	44-45	0.2368461173533305
1102	46-47	0.26823697105985644
1102	48-49	0.32290386862329257
1102	50-51	0.3549485405367321
1102	52-53	0.35809213579407384
1102	54-55	0.3195956127009154
1102	56-57	0.26907648356168323
1102	58-59	0.32143208377591037
1102	60-61	0.26436027058696965
1102	62-63	0.24764284981510087
1102	64-65	0.2567998845212216
1102	66-67	0.2887689008800507
1102	68-69	0.2229956531817976
1102	70-71	0.2961555629315171
1102	72-73	0.28030433303450053
1102	74-75	0.18204769389921438
1102	76-77	0.19372578829310072
1102	78-79	0.1797701536267553
1102	80-81	0.21883277371586018
1102	82-83	0.18740358696959447
1102	84-85	0.24454722105991067
1102	86-87	0.2446574952399061
1102	88-89	0.21580601779832875
1102	90-91	0.19208248750050672
1102	92-93	0.15490755126455014
1102	94-95	0.17678128681982486
1102	96-97	0.23715021431697636
1102	98-99	0.15601756484129226
1102	100-101	0.21136846068456805
2203	1	0.028466925010867783
2203	2	0.04282584892526131
2203	3	0.051331913809804064
2203	4	0.03420603108077103
2203	5	0.05570477697314402
2203	6	0.0842118267079357
2203	7	0.07302363059739747
2203	8	0.059778975832962544
2203	9	0.08396842262022375
2203	10-11	0.10908100166560786
2203	12-13	0.1105937860213757
2203	14-15	0.18311594700366385
2203	16-17	0.16021086447418043
2203	18-19	0.18605972257653747
2203	20-21	0.17507132939743997
2203	22-23	0.20818571568295852
2203	24-25	0.18703240078029637
2203	26-27	0.18683230992243693
2203	28-29	0.18355210551407453
2203	30-31	0.21387361254343773
2203	32-33	0.2024815583494899
2203	34-35	0.20480159800820275
2203	36-37	0.20890201499638295
2203	38-39	0.156154366911899
2203	40-41	0.18159464176113715
2203	42-43	0.12581569093715927
2203	44-45	0.18008803068831014
2203	46-47	0.18279312936324743
2203	48-49	0.15347819001772933
2203	50-51	0.21036474779208447
2203	52-53	0.2153777674421491
2203	54-55	0.17833950810123156
2203	56-57	0.09140218035307868
2203	58-59	0.19683909888129136
2203	60-61	0.1367404713848046
2203	62-63	0.1196319566195001
2203	64-65	0.15606702394224925
2203	66-67	0.14677728429195014
2203	68-69	0.08720073594940914
2203	70-71	0.10098968023885391
2203	72-73	0.1347295426355828
2203	74-75	0.1106936325665302
2203	76-77	0.12474165244102409
2203	78-79	0.12003971615197173
2203	80-81	0.12193229651130366
2203	82-83	0.0669659054004299
2203	84-85	0.11372632118855819
2203	86-87	0.051490798603879284
2203	88-89	0.045263842378133745
2203	90-91	0.04818371352445894
2203	92-93	0.02713323611239815
2203	94-95	0.10532436608480822
2203	96-97	0.11134421796309368
2203	98-99	0.07415458897961003
2203	100-101	0.12815404380714313
1103	1	0.037829789901770994
1103	2	0.04674217080191312
1103	3	0.04268693198215345
1103	4	0.04974634513644105
1103	5	0.0731073900602297
1103	6	0.10374568452000688
1103	7	0.0017378871943378726
1103	8	0.0724128228786114
1103	9	0.06322740620397127
1103	10-11	0.08231297767434143
1103	12-13	0.09274884139107797
1103	14-15	0.1740331996157778
1103	16-17	0.1669907751101931
1103	18-19	0.09110602968477366
1103	20-21	0.15786690250133262
1103	22-23	0.12677165661081347
1103	24-25	0.07842382663172032
1103	26-27	0.14345028112524716
1103	28-29	0.09987299826886442
1103	30-31	0.01166272171134608
1103	32-33	0.1510987476697565
1103	34-35	0.10153195333222698
1103	36-37	0.11601757742018037
1103	38-39	0.14272624171118053
1103	40-41	0.1138503971788154
1103	42-43	0.12112813425177649
1103	44-45	0.12181310677171098
1103	46-47	0.17001081605211965
1103	48-49	0.2097712406277168
1103	50-51	0.2693679553647499
1103	52-53	0.23347572886482482
1103	54-55	0.18086996395975774
1103	56-57	0.04478331203166874
1103	58-59	0.1429833528779625
1103	60-61	0.17017755558342884
1103	62-63	0.12481520507054711
1103	64-65	0.16973464487269752
1103	66-67	0.08121247332366721
1103	68-69	0.01219009665902604
1103	70-71	0.09744941481842773
1103	72-73	0.11417038367832788
1103	74-75	0.16634717344921057
1103	76-77	0.1885461574946774
1103	78-79	0.1837361369091255
1103	80-81	0.15941400475843182
1103	82-83	0.15660952012039786
1103	84-85	0.160228603463743
1103	86-87	0.17815644839109268
1103	88-89	0.1937449471896997
1103	90-91	0.12664426287914665
1103	92-93	0.03561071567834517
1103	94-95	0.14386296578373958
1103	96-97	0.2243171185136532
1103	98-99	0.2009152204808231
1103	100-101	0.25952457044139976
2204	1	0.019916683692720483
2204	2	0.022258940460091026
2204	3	0.03000583274330637
2204	4	0.009643769379295009
2204	5	0.07647523964501346
2204	6	0.05440206315042673
2204	7	0.02748145078628994
2204	8	0.07458324780915149
2204	9	0.0688500106388048
2204	10-11	0.06705832031384062
2204	12-13	0.06273711595174092
2204	14-15	0.03787417793331116
2204	16-17	0.0986023977015833
2204	18-19	0.10784631282198376
2204	20-21	0.11275314807849668
2204	22-23	0.13653046131686608
2204	24-25	0.12923382472428102
2204	26-27	0.11628019564859216
2204	28-29	0.16496000084144669
2204	30-31	0.13214567236539665
2204	32-33	0.14609813358837442
2204	34-35	0.18132307984306806
2204	36-37	0.14800889273743678
2204	38-39	0.1378395990762442
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1213	50-51	-0.7395207065724705
1213	52-53	-0.6685588857489222
1213	54-55	-0.6558952606133559
1213	56-57	-0.6790094062931118
1213	58-59	-0.6264732357909963
1213	60-61	-0.6616625417947333
1213	62-63	-0.8297330697958074
1213	64-65	-0.7612837668049295
1213	66-67	-0.7585451948800284
1213	68-69	-0.7741183844231756
1213	70-71	-0.8779986854194988
1213	72-73	-0.9052718338968972
1213	74-75	-0.5126250364910732
1213	76-77	-0.5402237122936455
1213	78-79	-0.5994982739081323
1213	80-81	-0.48537000752345705
1213	82-83	-0.4863112891088832
1213	84-85	-0.3844382449774031
1213	86-87	-0.5093450448928323
1213	88-89	-0.5471090679534427
1213	90-91	-0.5428348076869582
1213	92-93	-0.6100082752531577
1213	94-95	-0.6671790249546703
1213	96-97	-0.7050458693716664
1213	98-99	-0.7528689984487613
1213	100-101	-0.6835598237394684
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	14.0
10	43.0
11	129.0
12	363.0
13	1118.0
14	3592.0
15	8181.0
16	14411.0
17	22956.0
18	36463.0
19	55471.0
20	79946.0
21	111572.0
22	151642.0
23	202738.0
24	268425.0
25	353374.0
26	463479.0
27	616694.0
28	864558.0
29	1501174.0
30	1359567.0
31	1011222.0
32	1311001.0
33	1787285.0
34	2447408.0
35	3354062.0
36	5743782.0
37	1.7536402E7
38	9831129.0
39	5601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.01421110060596	11.055448483424628	13.29971824967124	36.630622166298174
2	20.888451759077263	22.757505244048563	39.01113273585551	17.342910261018655
3	21.817219240165237	21.171948429046576	30.783170786338825	26.22766154444936
4	25.059937870093695	31.056174832209265	19.087781257474873	24.79610604022216
5	28.5366870885182	32.03873560945686	20.943414895109104	18.481162406915846
6	21.896306157001884	34.34358816945493	24.9846933911609	18.775412282382288
7	14.035583935757517	20.464742198940435	42.576497688772776	22.92317617652927
8	18.245217276114456	23.506122490922305	28.07155652680943	30.177103706153808
9	28.915689373258747	19.779896977859163	28.079349981142077	23.225063667740013
10-11	25.179317108229615	34.063226280504736	19.746492970985866	21.010963640279787
12-13	21.400835759818577	24.563655904071204	28.204002361722896	25.83150597438732
14-15	22.244425314645458	27.590116394121296	26.660272846190885	23.50518544504236
16-17	25.394462796709362	25.01922216105479	25.681955839066404	23.90435920316944
18-19	21.75227929038623	24.913524195237308	27.72366787072486	25.610528643651598
20-21	25.368530173843883	24.78113263716556	27.803036415271464	22.04730077371909
22-23	22.310267944464066	31.136626240089427	24.481864029010627	22.071241786435884
24-25	21.838465702849017	25.762803673830305	27.59933830927418	24.7993923140465
26-27	22.798170593424746	24.606140082152773	25.591167594776188	27.004521729646292
28-29	24.729452116397358	27.66874479944528	24.822504553779282	22.779298530378075
30-31	21.729126452569535	25.25984248956273	27.8410000872242	25.170030970643538
32-33	21.115822202457544	27.83662357382819	25.212162410666465	25.835391813047803
34-35	23.46512964115082	26.41973211996239	25.74404398085075	24.371094258036038
36-37	21.742992621923353	28.8264740413736	27.79331316961022	21.637220167092824
38-39	24.515018275260548	24.872068699506684	28.754391783501948	21.858521241730816
40-41	21.931770090041606	28.830648697658784	28.624676321856423	20.612904890443186
42-43	21.575598891115078	25.247763282692336	28.8902046877425	24.286433138450086
44-45	24.793797820671344	26.20855429900172	25.8165329969271	23.181114883399843
46-47	22.488219949102394	27.47655519813228	28.16261647416556	21.872608378599768
48-49	24.744950107829382	24.963812930578204	28.029906049707975	22.26133091188444
50-51	21.629350300699475	24.97019473708348	25.49348018304159	27.906974779175453
52-53	25.389717934552024	24.97305101352486	28.17144304817486	21.46578800374826
54-55	22.602835865616616	24.71290688689146	27.761869903024245	24.92238734446768
56-57	21.37358801140098	25.00904217436576	28.527070416798328	25.090299397434933
58-59	25.138635601950334	25.020487054776936	28.624963848020467	21.215913495252263
60-61	22.125923431572282	25.822382776773157	28.283946453998137	23.76774733765642
62-63	23.89978439854643	26.1891608679088	28.379115232199958	21.531939501344812
64-65	21.133441913487804	32.091233177974686	25.11336631498097	21.661958593556534
66-67	22.673047213649816	31.83151269766259	23.96748277521438	21.527957313473216
68-69	21.33355244796554	31.304437245283438	25.971165120792385	21.390845185958636
70-71	21.577104254397085	30.627229140439425	25.529473426481395	22.26619317868209
72-73	21.969719329868177	30.980942690148655	24.76181147879844	22.287526501184733
74-75	22.403007725066317	30.058565671681286	26.060289839064993	21.4781367641874
76-77	23.117717075743403	28.09656627009777	26.014917619212692	22.770799034946133
78-79	23.42461086185025	28.151861590326156	25.143047560197235	23.28047998762636
80-81	24.47467981354535	27.47846575043414	25.512434973374187	22.534419462646323
82-83	23.542545686565617	27.632903331853083	25.929719581703836	22.894831399877464
84-85	23.13993266781219	28.51905561964309	24.923431321549987	23.417580390994733
86-87	22.275209468488164	29.11670260813833	25.7399370162198	22.86815090715371
88-89	22.907005441597008	27.926115986469753	26.99482184654846	22.172056725384774
90-91	23.59721760973153	27.023681772148194	26.80082288518346	22.57827773293681
92-93	22.773526050721024	26.63663333429018	27.78820537749786	22.80163523749093
94-95	23.172186370661592	27.07217138404535	25.780430483021654	23.9752117622714
96-97	22.067584224702184	29.345462251662745	25.366817235278898	23.220136288356173
98-99	23.472005302716976	28.258278393129586	25.66717111559335	22.602545188560086
100-101	22.995717609791477	26.881975404910197	26.11071783897177	24.011589146326557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2301.0
1	1936.5
2	1427.0
3	1351.0
4	1434.0
5	1509.0
6	1637.5
7	1752.0
8	1889.0
9	2043.0
10	2220.5
11	2477.5
12	2765.5
13	3146.5
14	3711.5
15	5006.0
16	8647.5
17	13306.5
18	17262.5
19	17430.0
20	16887.0
21	20327.5
22	26063.5
23	32406.5
24	42652.0
25	71264.5
26	116712.0
27	159306.5
28	161711.5
29	139703.5
30	143985.0
31	167486.5
32	195630.5
33	230511.0
34	256691.0
35	321927.0
36	586791.0
37	824875.5
38	921281.0
39	1021512.5
40	1228438.0
41	1590040.5
42	1990336.0
43	2137476.0
44	2239534.0
45	2673355.5
46	3271929.5
47	3632138.0
48	3929003.0
49	4157568.0
50	3661493.0
51	3045986.5
52	2591571.0
53	2160171.5
54	1772919.0
55	1142763.0
56	671373.5
57	420344.5
58	209116.0
59	136841.0
60	113711.5
61	132764.5
62	213104.0
63	211653.0
64	130181.5
65	68636.5
66	35931.5
67	21675.5
68	2081.5
69	862.0
70	397.0
71	177.5
72	106.5
73	78.0
74	56.5
75	41.5
76	33.5
77	26.0
78	16.0
79	9.0
80	8.5
81	5.5
82	6.0
83	7.0
84	3.5
85	2.0
86	1.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022989672859071452
2	0.0
3	0.006189996887705378
4	0.0
5	0.010046027164563265
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.273173393879453E-5
14-15	0.0
16-17	1.4243911312931507E-5
18-19	2.0348444732759297E-6
20-21	0.0
22-23	4.171431170215656E-5
24-25	0.0
26-27	0.0
28-29	1.8313600259483367E-5
30-31	6.745509428909707E-4
32-33	0.0023319317663742156
34-35	1.0886417932026225E-4
36-37	2.0958898074742078E-4
38-39	9.360284577069277E-5
40-41	1.6075271338879843E-4
42-43	4.476657841207045E-5
44-45	9.563769024396869E-5
46-47	4.792058734564814E-4
48-49	0.0060607842636523565
50-51	0.05847634305076703
52-53	0.0019432764719785128
54-55	1.6278755786207436E-4
56-57	9.797776138823601E-4
58-59	0.04338695385918937
60-61	0.009510863068091695
62-63	0.013928510419573738
64-65	0.022025156578738662
66-67	0.005641606302157515
68-69	0.0011771575277901253
70-71	5.087111183189824E-6
72-73	2.3400711442673192E-5
74-75	1.7194435799181606E-4
76-77	0.001305352729606509
78-79	0.0012646558401409904
80-81	0.004484797219100149
82-83	0.004695403622084208
84-85	0.006343627645437711
86-87	0.00428436503848247
88-89	0.004779849667725158
90-91	7.640840997151117E-4
92-93	0.013927492997337101
94-95	0.013488984013346137
96-97	8.719308567987358E-4
98-99	0.007555377529273527
100-101	0.0025893395922436204
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4.9143805E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.644932945710929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.04229672210971	6.833153550376253
2	11.7357070313513	2.029088007130805
3	3.435549167634327	0.8910027655767525
4	1.6465399803087988	0.5693691088880702
5	0.8477848554216731	0.36645216137547987
6	0.5538474789460888	0.287278459058399
7	0.4175178727804494	0.25265898096759953
8	0.35823865140416483	0.24775592959607337
9	0.1974699840714227	0.15364032939792488
>10	1.2736988408906278	2.028036029152369
>50	0.1328895885391638	0.8187182650069301
>100	0.18254897168145337	3.5555627501009344
>500	0.0462321463571859	2.8593946412589353
>1k	0.08734692455073154	17.733050606663724
>5k	0.0216224195995678	13.08204449756074
>10k+	0.02070936435259913	48.292793917889156
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC	2528543	5.145191748990539	TruSeq Adapter, Index 2 (100% over 50bp)
CTCAACTTCATGCGGCTAAACACCGCTTGTCCCTCTAAGAAGTTGTCTGG	142483	0.28993074508577427	No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA	142349	0.28965807592635534	No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG	133787	0.2722357375461668	No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC	132985	0.27060379227859954	No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG	123390	0.251079459557517	No Hit
GAACAATCCAACGCTTGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCG	122798	0.24987483162933763	No Hit
GTCAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTC	119682	0.24353425625060982	No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC	117124	0.23832912408797	No Hit
GTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTGGAT	113774	0.23151239510249563	No Hit
GTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAATTAAAA	113321	0.23059061055610164	No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG	102435	0.20843929362001987	No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT	100640	0.20478674779048955	No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA	99084	0.2016205297900722	No Hit
CGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGT	97106	0.19759560742193244	No Hit
CTCGGTTCTATTTTGTTGGTTTTCTGATCTGAGATAATGGTTAAGAGGGA	96617	0.1966005684745005	No Hit
CTGGAATCGGTTCAGCCGGAGATAGGGATGTTGTTTCCGTAAAGCGCCAC	96588	0.19654155798477552	No Hit
CTCACTTCACATTACAGCCCGACCGGCCCAGTCCTTAGAGCCAATCCTTA	92849	0.18893327449919678	No Hit
GCCAGATCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTA	91828	0.1868556982919821	No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC	90446	0.18404354322991473	No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC	90391	0.18393162678388456	No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA	89901	0.18293455299197936	No Hit
CTTAGATGTCCAGGGCTGCACGCGCGCTACACTGGAGGAATCAGCGTGCT	87679	0.17841312857236025	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC	86837	0.1766997895258619	No Hit
CGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAG	84339	0.17161674803161864	No Hit
CTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTGGATC	75874	0.1543917895653379	No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA	75566	0.1537650574675689	No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG	75436	0.15350052768604303	No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG	74184	0.15095290240550158	No Hit
CGAAATTCAAATAGCTTCCCTCGGATTTTCATGGATCGAACAAAGCGCAC	72467	0.1474590744448868	No Hit
CTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAGAAT	71678	0.1458535821554721	No Hit
GTTGAAGCTTCCATTGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAG	71001	0.1444759924470643	No Hit
ATTTTTTCAAGGTAAACTTGCTAGCCACCATATGCAACCAATTAAGGTAA	69738	0.1419059838773168	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG	68447	0.13927899966231758	No Hit
CTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAATCTCGTT	67005	0.13634475393185366	No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT	64991	0.13224657716267596	No Hit
CTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGCACATGAACCA	64597	0.13144484844020524	No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT	64525	0.13129833963812937	No Hit
CTCGCTCGTTATCGGAATAAACCAGACAAATCACTCCACCAACTAAGAAC	64497	0.13124136399287764	No Hit
GCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAG	64481	0.13120880648130523	No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT	61107	0.12434324122847225	No Hit
CGGAGATAGGGATGTTGTTTCCGTAAAGCGCCACGGTTCCTGTGGTGTCT	60564	0.1232383206794834	No Hit
CTTACCTACATTATTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGC	59765	0.12161247994533594	No Hit
GCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTCTTCATTAATCA	59069	0.1201962281919359	No Hit
GTCGCAATGGTTAGCGTTGAAGACTTTGGGCGTAGGCCCGGTTGAAGCTT	58918	0.11988896667647123	No Hit
CGAGACTCTAGCCTACTAAATAGTTACTGGATAATTTTATCGTCCAGACA	58687	0.11941891760314449	No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT	57815	0.11764453322244788	No Hit
CCCACTTATGCTACACCTCTCATGACTCTTCACAGAGTCGGACTAGAGTC	57809	0.11763232415560822	No Hit
GATAAATCGAAAGGGAAACGGGTTAATATTCCCGTACCTGGACACGGAGA	57000	0.115986134976728	No Hit
TTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCCACTTAT	56689	0.11535329834553919	No Hit
GTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCG	56096	0.11414663557288654	No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCC	55712	0.1133652552951486	No Hit
TTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAGTGAGAGGA	55555	0.11304578471284428	No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA	55423	0.11277718524237185	No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG	54885	0.1116824389157494	No Hit
GGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGAC	54704	0.11131413206608647	No Hit
GGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTCGT	53314	0.10848569824823291	No Hit
GTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGGCAACCA	53307	0.10847145433691997	No Hit
CTTGGACCGTAGCGAGACGTACGACTGCGAAAGCATTTGCCAAGAATGTC	53279	0.10841447869166826	No Hit
CGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAA	53156	0.10816419282145531	No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG	52405	0.1066360246220251	No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA	52224	0.10626771777236216	No Hit
TTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCCACCAAAGAATC	52122	0.106060163636088	No Hit
CTTTTCTCGAAACGGCTCAGTCCCGGGCAGCGACGGGCGGTGTGTACAAA	51612	0.10502239295471728	No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA	51439	0.10467036486084054	No Hit
CTCGCACCGAAGACAGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC	50950	0.10367532591340863	No Hit
GGTAATTCCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGC	50584	0.10293057283618963	No Hit
GGGCATTCGTATCGCTGCGTGAGAGGTGAAATTCTTGGACCGTAGCGAGA	50473	0.102704705099656	No Hit
CTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATAGAACCGAGATC	50283	0.10231808464973358	No Hit
CCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTG	49840	0.10141664854807232	No Hit
GGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTTC	49789	0.10131287147993526	No Hit
GGGAGTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCT	49477	0.10067800000427317	No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT	49251	0.10021812515331281	No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT	49203	0.10012045261859556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0019290325606655812	4.0696889465518594E-6	0.0
2	0.0020328096288026536	4.0696889465518594E-6	6.104533419827789E-6
3	0.0022139107869242115	6.104533419827789E-6	6.104533419827789E-6
4	0.0024397785234578397	6.104533419827789E-6	6.104533419827789E-6
5	0.0027673884836552642	6.104533419827789E-6	6.104533419827789E-6
6	0.0029138972857311314	1.2209066839655578E-5	1.0174222366379648E-5
7	0.0030624409322802743	1.2209066839655578E-5	1.0174222366379648E-5
8	0.0032598208461880394	1.2209066839655578E-5	1.0174222366379648E-5
9	0.004210093215207898	1.2209066839655578E-5	1.2209066839655578E-5
10-11	0.005073884694113531	1.5261333549569473E-5	1.4243911312931509E-5
12-13	0.00573419172569157	1.8313600259483367E-5	1.6278755786207438E-5
14-15	0.006735335206543328	1.933102249612133E-5	1.7296178022845402E-5
16-17	0.007787349799226984	2.0348444732759295E-5	2.0348444732759295E-5
18-19	0.008585008832751147	2.0348444732759295E-5	2.0348444732759295E-5
20-21	0.0092778733759016	2.0348444732759295E-5	2.0348444732759295E-5
22-23	0.010035852942196884	2.0348444732759295E-5	2.0348444732759295E-5
24-25	0.01092304513254519	2.0348444732759295E-5	2.0348444732759295E-5
26-27	0.012399324797906877	2.0348444732759295E-5	2.1365866969397263E-5
28-29	0.014679368030212556	2.1365866969397263E-5	2.2383289206035228E-5
30-31	0.02221134484804341	2.4418133679311157E-5	2.2383289206035228E-5
32-33	0.026614748288212524	2.543555591594912E-5	2.3400711442673192E-5
34-35	0.032072201165538564	2.6452978152587086E-5	2.4418133679311157E-5
36-37	0.03879837957195215	2.6452978152587086E-5	2.4418133679311157E-5
38-39	0.046662036038926984	2.8487822625863014E-5	2.4418133679311157E-5
40-41	0.05624513608581998	2.950524486250098E-5	2.4418133679311157E-5
42-43	0.06782136629428673	3.052266709913895E-5	2.4418133679311157E-5
44-45	0.0813591865749915	3.052266709913895E-5	2.6452978152587086E-5
46-47	0.09774578911828255	3.154008933577691E-5	2.6452978152587086E-5
48-49	0.11698626103534311	3.357493380905284E-5	2.6452978152587086E-5
50-51	0.14016313958595594	3.4592356045690804E-5	2.6452978152587086E-5
52-53	0.16672599934009996	3.4592356045690804E-5	2.747040038922505E-5
54-55	0.19914310664385876	3.4592356045690804E-5	2.8487822625863014E-5
56-57	0.23337326037330647	3.4592356045690804E-5	2.8487822625863014E-5
58-59	0.2695629693305189	3.4592356045690804E-5	3.4592356045690804E-5
60-61	0.31216752548973364	3.967946722888063E-5	4.069688946551859E-5
62-63	0.3607758902673491	4.4766578412070456E-5	4.273173393879453E-5
64-65	0.4176782811180372	4.4766578412070456E-5	4.3749156175432494E-5
66-67	0.48261932506040184	4.781884512198435E-5	4.4766578412070456E-5
68-69	0.5572981172296285	4.883626735862231E-5	5.087111183189824E-5
70-71	0.6407877859681398	4.883626735862231E-5	5.087111183189824E-5
72-73	0.741156489612475	4.883626735862231E-5	5.087111183189824E-5
74-75	0.8602101526326664	4.9853689595260274E-5	5.087111183189824E-5
76-77	0.9833029819323107	5.188853406853621E-5	5.290595630517417E-5
78-79	1.1264624300051653	5.290595630517417E-5	5.595822301508807E-5
80-81	1.294996388659771	5.49408007784501E-5	5.7993067488363997E-5
82-83	1.4777376314267892	5.49408007784501E-5	6.0027911961639925E-5
84-85	1.68201566809896	5.697564525172603E-5	6.206275643491586E-5
86-87	1.91265206265571	5.697564525172603E-5	6.308017867155382E-5
88-89	2.167342353731055	5.697564525172603E-5	6.816728985474364E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCAAT	14355	0.0	70.461716	1
TCCAATC	14765	0.0	68.489494	2
AAGAGCA	402345	0.0	66.56595	7
CGGAAGA	423115	0.0	63.52454	4
GAGCACA	422845	0.0	63.285973	9
TCGGAAG	430460	0.0	62.58163	3
AGAGCAC	427835	0.0	62.50233	8
GATCGGA	452415	0.0	59.8071	1
GAAGAGC	460670	0.0	58.192734	6
TCAAAGG	18585	0.0	57.086143	7
GGAAGAG	470995	0.0	57.00934	5
ATCAAAG	19310	0.0	54.623108	6
CCAATCA	18740	0.0	54.39286	3
CAAAGGA	19610	0.0	52.068016	8
CTTGTTA	17805	0.0	51.578724	1
ATCGGAA	527655	0.0	51.1503	2
CAATCAA	21210	0.0	49.264557	4
CCGGCAT	3105	0.0	47.280014	1
CGCGGAT	2745	0.0	47.07684	1
GCCGTCT	289335	0.0	45.94852	48-49
>>END_MODULE